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Identification of a pancreatic beta-cell insulin gene transcription factor that binds to and appears to activate cell-type-specific expression: its possible relationship to other cellular factors that bind to a common insulin gene sequence. 总被引:21,自引:13,他引:8
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J Whelan S R Cordle E Henderson P A Weil R Stein 《Molecular and cellular biology》1990,10(4):1564-1572
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D J Drucker J Philippe L Jepeal J F Habener 《The Journal of biological chemistry》1987,262(32):15659-15665
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Cooperativity of sequence elements mediates tissue specificity of the rat insulin II gene. 总被引:9,自引:5,他引:4
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The 5'-flanking region of the rat insulin II gene (-448 to +50) is sufficient for tissue-specific expression. To further determine the tissue-specific cis-acting element(s), important sequences defined by linker-scanning mutagenesis were placed upstream of a heterologous promoter and transfected into insulin-producing and -nonproducing cells. Rat insulin promoter element 3 (RIPE3), which spans from -125 to -86, was shown to confer beta-cell-specific expression in either orientation. However, two subregions of RIPE3, RIPE3a and RIPE3b (defined by linker-scanning mutations), displayed only marginal activities. These results suggest that the two subregions cooperate to confer tissue specificity, presumably via their cognate binding factors. 相似文献
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