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1.
Environmental interactions and the effects of such interactions on the evolution of genome attributes is an intriguing area of ongoing research. Several earlier studies have delved into how the genome size (GS) and the guanine-cytosine content (GC) of genomes are shaped by species’ ecology while largely disregarding other genome attributes, such as number of chromosomes (CR), number of genes (GE), and protein count (PC) from such comparisons. The present study was designed at understanding the influence of ecology––climate, habitat, and depth––on genome attributes by using the most current data on 579 whole fish genomes available at NCBI. Given the diverse and intricate roles of GS and GC in species adaptations to temperature, salinity, and hydrostatic pressure, the focus was on finding if and how the genomes responded to these stressors and if any common patterns existed in the genome-level responses. Our analyses exhibited some significant and intriguing trends for fishes as a whole while indicating strong effects of ecology on GS, GC, CR, and PC. Also, some very unique trends were observed on regressing GS and GC values across temperature, salinity, and depth clines. Accordingly, a very strong decline in the GS and a concomitant increase in GC were observed in species through the tropics/sub tropics to the temperate/poles, from freshwater to the marine habitats, and from the pelagic to bathydemersal depths. Observed patterns strongly support the notion that smaller GS and larger GC are associated with species inhabiting more stable environments and vice versa. The results also signify the effect of these patterns on protein flexibility and its role in tolerating stressful conditions. Observed patterns are discussed in the light of latitudinal biodiversity gradient, habitat complexity, and energy and metabolic expenditure hypothesis. 相似文献
2.
The genomes of several vertebrates, including six mammals, the chicken, Xenopus and four ray-finned fishes have been sequenced or are currently being sequenced to provide a better understanding of the human genome through comparative analysis. However, this list does not include cartilaginous fishes, which are the most basal living jawed vertebrates [1]. The genomes of the current ‘popular’ cartilaginous fishes such as the nurse shark, dogfish, and horn shark are larger than the human genome (∼3800 Mb to 7000 Mb) [2], and are not attractive for whole-genome sequencing. Here, we report the characterization of the relatively small genome (1200 Mb) of a cartilaginous fish, the elephant fish (Callorhinchus milii), and propose it as a model for whole-genome sequencing. 相似文献
3.
昆虫种类繁多,它与生态系统中的生物多样性,以及人类的日常生活和生产密切相关。自2000年黑腹果蝇Drosophila melanogaster全基因组测序完成以来,至今已先后开展了88种昆虫全基因组测序工作,这标志着昆虫学研究进入了基因组时代。本文综述了近年来昆虫基因组测序进展,以及基于基因组的昆虫学研究方法及应用等两方面的研究成果。同时,着重介绍了昆虫全基因组测序进程,昆虫基因组在个体生物学、多物种间及种群,及系统生物学研究中的应用等方面的内容。最后,还探讨了基因组时代昆虫学研究所面临的挑战。 相似文献
4.
Alexandra Schmidt Clment Schneider Peter Decker Karin Hohberg Jrg Rmbke Ricarda Lehmitz Mikls Blint 《Ecology and evolution》2022,12(6)
- Metagenomics – shotgun sequencing of all DNA fragments from a community DNA extract – is routinely used to describe the composition, structure, and function of microorganism communities. Advances in DNA sequencing and the availability of genome databases increasingly allow the use of shotgun metagenomics on eukaryotic communities. Metagenomics offers major advances in the recovery of biomass relationships in a sample, in comparison to taxonomic marker gene‐based approaches (metabarcoding). However, little is known about the factors which influence metagenomics data from eukaryotic communities, such as differences among organism groups, the properties of reference genomes, and genome assemblies.
- We evaluated how shotgun metagenomics records composition and biomass in artificial soil invertebrate communities at different sequencing efforts. We generated mock communities of controlled biomass ratios from 28 species from all major soil mesofauna groups: mites, springtails, nematodes, tardigrades, and potworms. We shotgun sequenced these communities and taxonomically assigned them with a database of over 270 soil invertebrate genomes.
- We recovered over 95% of the species, and observed relatively high false‐positive detection rates. We found strong differences in reads assigned to different taxa, with some groups (e.g., springtails) consistently attracting more hits than others (e.g., enchytraeids). Original biomass could be predicted from read counts after considering these taxon‐specific differences. Species with larger genomes, and with more complete assemblies, consistently attracted more reads than species with smaller genomes. The GC content of the genome assemblies had no effect on the biomass–read relationships. Results were similar among different sequencing efforts.
- The results show considerable differences in taxon recovery and taxon specificity of biomass recovery from metagenomic sequence data. The properties of reference genomes and genome assemblies also influence biomass recovery, and they should be considered in metagenomic studies of eukaryotes. We show that low‐ and high‐sequencing efforts yield similar results, suggesting high cost‐efficiency of metagenomics for eukaryotic communities. We provide a brief roadmap for investigating factors which influence metagenomics‐based eukaryotic community reconstructions. Understanding these factors is timely as accessibility of DNA sequencing and momentum for reference genomes projects show a future where the taxonomic assignment of DNA from any community sample becomes a reality.
5.
随着高通量DNA测序技术的飞速发展,越来越多的物种完成了基因组测序.定位编码基因、确定编码基因结构是基因组注释的基本任务,然而以往的基因组注释方法主要依赖于DNA及RNA序列信息.为了更加精确地解读完成测序的基因组,我们需要整合多种类型的组学数据进行基因组注释.近年来,基于串联质谱技术的蛋白质组学已经发展成熟,实现了对蛋白质组的高覆盖,使得利用串联质谱数据进行基因组注释成为可能.串联质谱数据一方面可以对已注释的基因进行表达验证,另一方面还可以校正原注释基因,进而发现新基因,实现对基因组序列的重新注释.这正是当前进展较快的蛋白质基因组学的研究内容.利用该方法系统地注释已完成测序的基因组已成为解读基因组的一个重要补充.本文综述了蛋白质基因组学的主要研究内容和研究方法,并展望了该研究方向未来的发展. 相似文献
6.
鱼类特异的基因组复制 总被引:2,自引:0,他引:2
辐鳍鱼类是脊椎动物中种类最多、分布最广的类群,其基因组大小不等。过去的观点认为,在脊椎动物进化历程中曾发生了两次基因组复制。近期的系统基因组学研究资料进一步提出,在大约350百万年,辐鳍鱼还发生了第三次基因组复制,即鱼类特异的基因组复制(fish-specificgenomeduplication,FSGD),且发生的时间正处在“物种极度丰富”的硬骨鱼谱系(真骨总目)和“物种贫乏”的谱系(辐鳍鱼纲基部的类群)出现分歧的时间点,表明FSGD与硬骨鱼物种和生物多样性的增加有关。进一步开展鱼类比较基因组学和功能基因组学研究将进一步验证FSGD这一假说。 相似文献
7.
Kuraku S 《基因组蛋白质组与生物信息学报(英文版)》2011,9(3):97-103
Hox genes,responsible for regional specification along the anteroposterior axis in embryogenesis,are found as clusters in most eumetazoan genomes sequenced to date.Invertebrates possess a single Hox gene cluster with some exceptions of secondary cluster breakages, while osteichthyans (bony vertebrates) have multiple Hox clusters. In tetrapods, four Hox clusters,derived from the so-called two-round whole genome duplications (2R-WGDs),are observed.Overall,the number of Hox gene clusters has been regarded as a reliable marker of ploidy levels in animal genomes. In fact, this scheme also fits the situations in teleost fishes that experienced an additional WGD. In this review, I focus on cyclostomes and cartilaginous fishes as lineages that would fill the gap between invertebrates and osteichthyans.A recent study highlighted a possible loss of the HoxC cluster in the galeomorph shark lineage, while other aspects of cartilaginous fish Hox clusters usually mark their conserved nature. In contrast,existing resources suggest that the cyclostomes exhibit a different mode of Hox cluster organization.For this group of species,whose genomes could have differently responded to the 2R-WGDs from jawed vertebrates,therefore the number of Hox clusters may not serve as a good indicator of their ploidy level. 相似文献
8.
【目的】本研究旨在通过针尾部(Aculeata)昆虫线粒体基因组系统发育分析认知土蜂科(Scoliidae)的单系性及系统发育位置。【方法】利用Illumina Hiseq2500二代测序技术测序土蜂科3属5种的线粒体基因组,并进行注释和分析;基于针尾部昆虫36个线粒体基因组13个蛋白质编码基因(protein-coding genes, PCGs)和2个rRNA基因序列采用最大似然法(maximum likelihood, ML)和贝叶斯法(Bayesian inference, BI)法构建系统发育树。【结果】新测序的土蜂科5个线粒体基因组为五带波壁土蜂Colpa quinquecincta线粒体基因组(GenBank登录号:OM103696),齿石波壁土蜂Colpa tartara线粒体基因组(GenBank登录号:OM103697),厚大长腹土蜂Megacampsomeris grossa线粒体基因组(GenBank登录号:OM103796),台湾大长腹土蜂Megacampsomeris formosensis线粒体基因组(GenBank登录号:OM142776)和斯式土蜂Sc... 相似文献
9.
Survey Sequencing and Comparative Analysis of the Elephant Shark (Callorhinchus milii) Genome
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Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh Byrappa Venkatesh 《PLoS biology》2007,5(4):e101
Owing to their phylogenetic position, cartilaginous fishes (sharks, rays, skates, and chimaeras) provide a critical reference for our understanding of vertebrate genome evolution. The relatively small genome of the elephant shark, Callorhinchus milii, a chimaera, makes it an attractive model cartilaginous fish genome for whole-genome sequencing and comparative analysis. Here, the authors describe survey sequencing (1.4× coverage) and comparative analysis of the elephant shark genome, one of the first cartilaginous fish genomes to be sequenced to this depth. Repetitive sequences, represented mainly by a novel family of short interspersed element–like and long interspersed element–like sequences, account for about 28% of the elephant shark genome. Fragments of approximately 15,000 elephant shark genes reveal specific examples of genes that have been lost differentially during the evolution of tetrapod and teleost fish lineages. Interestingly, the degree of conserved synteny and conserved sequences between the human and elephant shark genomes are higher than that between human and teleost fish genomes. Elephant shark contains putative four Hox clusters indicating that, unlike teleost fish genomes, the elephant shark genome has not experienced an additional whole-genome duplication. These findings underscore the importance of the elephant shark as a critical reference vertebrate genome for comparative analysis of the human and other vertebrate genomes. This study also demonstrates that a survey-sequencing approach can be applied productively for comparative analysis of distantly related vertebrate genomes. 相似文献
10.
11.
Survey sequencing and comparative analysis of the elephant shark (Callorhinchus milii) genome
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Venkatesh B Kirkness EF Loh YH Halpern AL Lee AP Johnson J Dandona N Viswanathan LD Tay A Venter JC Strausberg RL Brenner S 《PLoS biology》2007,5(4):e101
Owing to their phylogenetic position, cartilaginous fishes (sharks, rays, skates, and chimaeras) provide a critical reference for our understanding of vertebrate genome evolution. The relatively small genome of the elephant shark, Callorhinchus milii, a chimaera, makes it an attractive model cartilaginous fish genome for whole-genome sequencing and comparative analysis. Here, the authors describe survey sequencing (1.4× coverage) and comparative analysis of the elephant shark genome, one of the first cartilaginous fish genomes to be sequenced to this depth. Repetitive sequences, represented mainly by a novel family of short interspersed element–like and long interspersed element–like sequences, account for about 28% of the elephant shark genome. Fragments of approximately 15,000 elephant shark genes reveal specific examples of genes that have been lost differentially during the evolution of tetrapod and teleost fish lineages. Interestingly, the degree of conserved synteny and conserved sequences between the human and elephant shark genomes are higher than that between human and teleost fish genomes. Elephant shark contains putative four Hox clusters indicating that, unlike teleost fish genomes, the elephant shark genome has not experienced an additional whole-genome duplication. These findings underscore the importance of the elephant shark as a critical reference vertebrate genome for comparative analysis of the human and other vertebrate genomes. This study also demonstrates that a survey-sequencing approach can be applied productively for comparative analysis of distantly related vertebrate genomes. 相似文献
12.
Sirijovski N Woolnough C Rock J Joss JM 《Journal of experimental zoology. Part B. Molecular and developmental evolution》2005,304(1):40-49
The genomes of lungfish, together with those of some urodele amphibians, are the largest of all vertebrate genomes. It has been assumed that the bulk of the DNA making up these large genomes has been derived from repeat elements, like the noncoding DNA of those genomes that have been sequenced (e.g., human). In an attempt to characterize repeat sequences in the lungfish genome, we have isolated, by restriction enzyme digestion of genomic DNA, sequences of a repeat element in Neoceratodus forsteri, the most primitive of the living lungfishes. The fragments sequenced from the EcoRI and BglII digests were used to perform genome walking PCR in order to obtain the full sequence of the repeat element. This element shares homology with the non-LTR (LINE) element, Chicken Repeat 1 (CR1), described for several vertebrates and some invertebrates; we have called it N. forsteri CR1 (NfCR1). NfCR1 shares all the domains of other CR1 elements but it also has several unique features that suggest it may no longer be active in the lungfish genome. It occurs in both full-length and 5'-truncated versions and in its present "inactive" form represents approximately 0.05% of the lungfish genome. 相似文献
13.
KS Lee RN Kim BH Yoon DS Kim SH Choi DW Kim SH Nam A Kim A Kang KH Park JE Jung SH Chae HS Park 《Bioinformation》2012,8(11):532-534
Recently, next generation sequencing (NGS) technologies have led to a revolutionary increase in sequencing speed and costefficacy. Consequently, a vast number of contigs from many recently sequenced bacterial genomes remain to be accurately mapped and annotated, requiring the development of more convenient bioinformatics programs. In this paper, we present a newly developed web-based bioinformatics program, Bacterial Genome Mapper, which is suitable for mapping and annotating contigs that have been assembled from bacterial genome sequence raw data. By constructing a multiple alignment map between target contig sequences and two reference bacterial genome sequences, this program also provides very useful comparative genomics analysis of draft bacterial genomes. AVAILABILITY: The database is available for free at http://mbgm.kribb.re.kr. 相似文献
14.
Isheng J. Tsai Martin Hunt Nancy Holroyd Thomas Huckvale Matthew Berriman Taisei Kikuchi 《DNA research》2014,21(3):243-254
Advances in both high-throughput sequencing and whole-genome amplification (WGA) protocols have allowed genomes to be sequenced from femtograms of DNA, for example from individual cells or from precious clinical and archived samples. Using the highly curated Caenorhabditis elegans genome as a reference, we have sequenced and identified errors and biases associated with Illumina library construction, library insert size, different WGA methods and genome features such as GC bias and simple repeat content. Detailed analysis of the reads from amplified libraries revealed characteristics suggesting that majority of amplified fragment ends are identical but inverted versions of each other. Read coverage in amplified libraries is correlated with both tandem and inverted repeat content, while GC content only influences sequencing in long-insert libraries. Nevertheless, single nucleotide polymorphism (SNP) calls and assembly metrics from reads in amplified libraries show comparable results with unamplified libraries. To utilize the full potential of WGA to reveal the real biological interest, this article highlights the importance of recognizing additional sources of errors from amplified sequence reads and discusses the potential implications in downstream analyses. 相似文献
15.
DNA sequencing has revolutionized yeast taxonomy. Although initially rDNA sequences proved to be universal and convenient for assigning phylogenetic relationships, it was eventually supplanted by multigene analysis, which provided more discriminating and robust results. This led to a new classification of the major yeast clades, which is still used as a reference today. More recently, the availability of a large number of complete genome sequences has given a new perspective on the molecular taxonomy of yeasts by providing a high number of genes to compare. It also highlighted an unexpected aspect of yeast genome evolution: the existence of interspecific hybrids outside of the industrial Saccharomyces clade. Together with the loss of heterozygosity in interspecific hybrids and a reduced sexuality leading to clonal propagation, this observation obliges us to reexamine the present concept of species. In parallel, the ongoing challenge is to find a universal molecular marker, to improve fast authentication and, if possible, phylogeny of yeasts. The future of yeast taxonomy will involve the sequencing of more genomes, thorough analysis of populations to obtain a good representation of the biodiversity and integration of these data into dedicated databases. 相似文献
16.
Berkman PJ Skarshewski A Lorenc MT Lai K Duran C Ling EY Stiller J Smits L Imelfort M Manoli S McKenzie M Kubaláková M Šimková H Batley J Fleury D Doležel J Edwards D 《Plant biotechnology journal》2011,9(7):768-775
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating chromosome arm 7DS and applied second‐generation technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions of this chromosome arm. The assembly represents approximately 40% of the chromosome arm and all known 7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce an annotated draft 7DS syntenic build, which is publicly available at http://www.wheatgenome.info . Our results suggest that the sequencing of isolated chromosome arms can provide valuable information of the gene content of wheat and is a step towards whole‐genome sequencing and variation discovery in this important crop. 相似文献
17.
复杂基因组测序技术研究进展 总被引:1,自引:0,他引:1
复杂基因组指的是无法使用常规测序和组装手段直接解析的一类基因组,通常指包含高比例重复序列、高杂合度、极端GC含量、存在难消除异源DNA污染的基因组。为了解决复杂基因组的测序和组装问题,需要分别从基因组测序实验方法、测序技术平台、组装算法与策略3个方面进行深入研究。本文详细介绍了复杂基因组测序组装相关的现有技术与方法,并结合复杂基因组经典实例介绍了复杂基因组测序的技术解决途径和发展历程,可为制订合适的复杂基因组测序策略提供参考。 相似文献
18.
昆虫基因组及其大小 总被引:5,自引:0,他引:5
昆虫基因组大小是由于基因组各种重复序列在扩增、缺失和分化过程中所致的数量差异造成的。这些差异使得昆虫不同类群间、种间和同种的不同种群间表现出基因组大小的不同。目前有59种昆虫已经列入基因组测序计划, 其中6种昆虫(黑腹果蝇Drosophila melanogaster、冈比亚按蚊Anopheles gambiae、家蚕Bombyx mori、意大利蜜蜂Apis mellifera、埃及伊蚊Aedes aegypti和赤拟谷盗Tribolium castaneum)的全基因组序列已经报道。有725种昆虫的基因组大小得到了估计, 大小在0.09~16.93 pg (88~16 558 Mb)之间。本文还介绍了昆虫基因组大小的估计方法, 讨论了昆虫基因组大小的变化及其意义。 相似文献
19.
Michael J Moore Amit Dhingra Pamela S Soltis Regina Shaw William G Farmerie Kevin M Folta Douglas E Soltis 《BMC plant biology》2006,6(1):17-13
Background
Plastid genome sequence information is vital to several disciplines in plant biology, including phylogenetics and molecular biology. The past five years have witnessed a dramatic increase in the number of completely sequenced plastid genomes, fuelled largely by advances in conventional Sanger sequencing technology. Here we report a further significant reduction in time and cost for plastid genome sequencing through the successful use of a newly available pyrosequencing platform, the Genome Sequencer 20 (GS 20) System (454 Life Sciences Corporation), to rapidly and accurately sequence the whole plastid genomes of the basal eudicot angiosperms Nandina domestica (Berberidaceae) and Platanus occidentalis (Platanaceae). 相似文献20.