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Extremely low frequency magnetic fields interact with an animal by inducing internal electric fields, which are in addition to the normal endogenous fields present in living animals. Male rats weighing about 560 g each were anesthetized with ketamine and xylazine. Small incisions were made in the ventral body wall at the chest and upper abdomen to position a miniature probe for measuring internal electric fields. The calibration constant for the probe size was 5.7 mm, with a flat response from at least 12 Hz to 20 kHz. A cardiac signal, similar to the normal electrocardiogram with a heart rate of about 250 bpm, was readily obtained at the chest. Upon analysis of its spectrum, the cardiac field detected by the probe had a broad maximum at 32–95 Hz. When the rats were exposed to a 1 mT, 60 Hz magnetic field, a spike appeared in the spectrum at 60 Hz. The peak-to-peak magnitudes of electric fields associated with normal heart function were comparable to fields induced by a 1 mT magnetic field at 60 Hz for those positions measured on the body surface (where induced fields were maximal). Within the body, or in different directions relative to the applied field, the induced fields were reduced (reaching zero at the center of the animal). The cardiac field increased near the heart, becoming much larger than the induced field. Thus, the cardiac electric field, together with the other endogenous fields, combine with induced electric fields and help to provide reference levels for the induced-field dosimetry of ELF magnetic field exposures of living animals. Bioelectromagnetics 18:317–323, 1997. © 1997 Wiley-Liss, Inc.  相似文献   

3.
  总被引:1,自引:0,他引:1  
A suppression in melatonin secretion is one of the mechanisms proposed to explain the possible adverse effects of extremely low frequency magnetic fields (ELF-MF), but the results of research are inconclusive. This study investigated the effect of occupational ELF-MF exposure on 6-sulfatoximelatonin (6-OHMS). Exposure was monitored for three complete work shifts in 59 workers using personal exposure meters. Environmental exposure was also evaluated. Urinary 6-OHMS in morning samples, an indicator of night-time melatonin production, was measured. Urine was collected twice on Friday and the following Monday. Workers were classified according to ELF exposure as low exposed (0.2 microT): 6-OHMS did not differ between groups (P > .05) in either Friday or Monday urine samples. In addition, 6-OHMS was not related to exposure under multivariate analysis. The ratio between 6-OHMS in Monday versus Friday samples was also calculated to test the hypothesis of a possible variation in pineal function after 2 days, interruption of occupational ELF-MF exposure: again no exposure-related difference was observed. Our results do not support the hypothesis that occupational exposure to ELF-MF significantly influences melatonin secretion.  相似文献   

4.
    
The ongoing Childhood Leukemia Survival Study is examining the possible association between magnetic field exposure and survival of children with newly diagnosed acute lymphocytic leukemia (ALL). We report the results of the first year 24 h personal magnetic field monitoring for 356 US and Canadian children by time weighted average TWA and alternative exposure metrics. The mean TWA of 0.12 microT was similar to earlier personal exposure studies involving children. A high correlation was found between 24 h TWA and alternative metrics: 12 h day TWA, 12 night TWA, geometric mean, 95th percentile value, percentage time over 0.2 and 0.3 microT, and an estimate of field stability (Constant Field Metric). Two measures of field intermittency, rate of change metric (RCM) and standardized rate of change metric (RCMS), were not highly correlated with TWA. The strongest predictor of TWA was location of residence, with highest TWAs associated with urban areas. Residence in an apartment, lower paternal educational level, and residential mobility were also associated with higher TWAs. There were no significant differences in the appliance use patterns of children with higher TWA values. Children with the highest field intermittency (high RCM) were more likely to sit within 3 feet of a video game attached to the TV. Our results suggest that 24 h TWA is a representative metric for certain patterns of exposure, but is not highly correlated with two metrics that estimate field intermittency.  相似文献   

5.
Residential magnetic and electric fields   总被引:1,自引:0,他引:1  
A magnetic flux density (MFD) and electric-field (E-field) data-acquisition system was built for characterizing extremely low-frequency fields in residences. Every 2 min during 24-h periods, MFD and E-field measurements were made in 43 homes in King, Pierce, and Snohomish counties of Washington State. The total electrical energy used in each residence during the 24-h measurement period was also recorded, and maps were drawn to scale of the distribution wiring within 43 m (140 ft) of these homes. Finally, on a separate date, field measurements were made in each home during an epidemiological interview. The results of this study can be summarized as follows: 1) 24-h-average MFD measured at two separate points in the family room were correlated, as were a 24-h-average bedroom measurement and the mean of the two family-room measurements. 2) The 24-h-average family-room MFD and E-field measurements were uncorrelated. 3) The 24-h-average total harmonic distortions of family-room MFD and E-fields were less than about 24% and 7%, respectively. 4) Residential MFD exhibited a definite 24-h (diurnal) cycle. 5) The 24-h-average and interviewer-measured MFD were correlated. 6) Residential 24-h-average MFD were correlated with the wiring code developed by Wertheimer and Leeper. 7) An improved prediction of 24-h-average residential MFD was obtained using the total number of service drops, the distance to neighboring transmission lines, and the number of primary phase conductors.  相似文献   

6.
A commercially available spreadsheet program is used on a microcomputer to calculate the induced current density and electric field patterns produced in a nonhomogeneous, anisotropic model of tissue by a localized, low-frequency magnetic field source. Specific application is made to coils used to promote the healing of bone fractures in limbs. The variation of the conductivity of the fracture gap during healing causes the induced current density pattern to change correspondingly, whereas the induced electric field remains relatively unchanged. Use of more simplified, isotropic models for the bone and for the soft tissue leads to results that differ significantly from those obtained from the full model. The magnetic field beyond the region of the coils contributes little to the induced currents in the fracture gap if the gap is located near the center of the coils. © 1994 Wiley-Liss, Inc.  相似文献   

7.
Considerable interest has developed during the past ten years regarding the hypothesis that living organisms may respond to temporal variability in ELF magnetic fields to which they are exposed. Consequently, methods to measure various aspects of temporal variability are of interest. In this paper, five measures of temporal variability were examined: Arithmetic means (D(mean)) and rms values (D(rms)) of the first differences (i.e., absolute value of the difference between consecutive measurements) of magnetic field recordings; "standardized" forms of D(rms), denoted RCMS, obtained by dividing D(rms) by the standard deviations of the magnetic field data; and mean (F(mean)) and rms (F(rms)) values of fractional first differences. Theoretical investigations showed that D(mean) and D(rms) are virtually unaffected by long-term systematic trends (changes) in exposure. These measures thus provide rather specific measures of short-term temporal variability. This was also true to a lesser extent for F(mean) and F(rms). In contrast, the RCMS metric was affected by both short-term and long-term exposure variabilities. The metrics were also investigated using a data set consisting of twice-repeated two-calendar-day recordings of bedroom magnetic fields and personal exposures of 203 women residing in the western portion of Washington State. The predominant source of short-term temporal variability in magnetic field exposures arose from the movement of subjects through spatially varying magnetic fields. Spearman correlations between TWA bedroom magnetic fields or TWA personal exposures and five measures of temporal variability were relatively low. Weak to moderate levels of correlation were observed between temporal variability measured during two different sessions separated in time by 3 or 6 months. We conclude that first difference and fractional difference metrics provide specific and fairly independent measures of short-term temporal variability. The RCMS metric does not provide an easily interpreted measure of short-term or long-term temporal variability. This last result raises uncertainties about the interpretation of published studies that use the RCMS metric.  相似文献   

8.
The development of a wire code protocol based on a study of electrical installations in Melbourne, Australia, is described. Because of very significant differences between the Melbourne power distribution system and that used in Denver, Colorado, an approach different from that used by Wertheimer and Leeper was required. A combined practical and theoretical approach was used to determine a continuous exposure index, defined as a measure of the potential for exposure due to external electrical installations. The protocol was tested on a convenient sample of 41 homes in which the field was monitored over a 12 hour overnight period. A correlation of 0.85 (95% CI 0.74–0.92, P < .0001) was obtained between the measured time-weighted average and the wire coding exposure index. To assess the efficacy of the wiring configuration index, a computer simulation of a case-control study was then performed. It was concluded that, using the same basic reasoning of the Wertheimer and Leeper code, it is possible to develop a location-specific code that provides a good correlation with the residential time-weighted average and an acceptable degree of exposure misclassification. © 1994 Wiley-Liss, Inc.  相似文献   

9.
Pregnant CD1 mice were exposed or sham-exposed from day 0 to day 17 of gestation to a 50 Hz sinusoidal magnetic field at 20 mT (rms). Preimplantation and postimplantation survival were assessed and fetuses examined for the presence of gross external, internal, and skeletal abnormalities. There were no statistically significant field-dependent effects on preimplantation or postimplantation survival, sex ratio, or the incidence of fetuses with internal or skeletal abnormalities. Magnetic field exposure was, however, associated with longer and heavier fetuses at term, with fewer external abnormalities. The results lend no support to suggestions of increased rates of spontaneous abortion or congenital malformation following prenatal exposure to power frequency magnetic fields. © 1994 Wiley-Liss, Inc.  相似文献   

10.
    
A programmable system has been developed for the study of both transient and persistent effects of extremely low frequency (ELF) magnetic field exposure of cell cultures. This high‐precision exposure system enables experimental blinding and fully characterized exposure while simultaneously allowing live cell imaging. It is based on a live imaging cell around which two asymmetrical coils are wound in good thermal contact to a temperature‐controlled water jacket, and is mounted on a microscope stage insert. The applied B‐field uniformity of the active volume is better than 1.2% with an overall exposure uncertainty of less than 4.3% with very low transient field levels. The computer‐controlled apparatus allows signal waveforms that are sinusoidal or composed of several harmonics, blind protocols, and monitoring of exposure and environmental conditions. B‐fields up to 4 mT root mean square amplitude are possible with minimal temperature variation and no recognizable temperature differences between exposure and sham states. Sources of artifacts have been identified and quantified. There are no visible vibrations observable even at the highest magnifications and exposure levels. Bioelectromagnetics 34:231–239, 2013. © 2012 Wiley Periodicals, Inc.  相似文献   

11.
    
Zeng Q  Ke X  Gao X  Fu Y  Lu D  Chiang H  Xu Z 《Bioelectromagnetics》2006,27(4):274-279
Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) at 0.2 mT. In the present study, we further explored the effects of incoherent noise MF on MF-induced GJIC inhibition. GJIC was determined by fluorescence recovery after photobleaching (FRAP) with a laser-scanning confocal microscope. The rate of fluorescence recovery (R) at 10 min after photobleaching was adopted as the functional index of GJIC. The R-value of NIH3T3 cells exposed to 50 Hz sinusoidal MF at 0.4 mT for 24 h was 30.85 +/- 14.70%, while the cells in sham exposure group had an R-value of 46.36 +/- 20.68%, demonstrating that the GJIC of NIH3T3 cells was significantly inhibited by MF exposure (P < .05). However, there were no significant differences in the R-values of the sham exposure, MF-plus-noise MF exposure (R: 49.58 +/- 19.38%), and noise MF exposure groups (R: 46.74 +/- 21.14%) (P > .05), indicating that the superposition of a noise MF alleviated the suppression of GJIC induced by the 50 Hz MF. In addition, although MF at an intensity of 0.2 mT synergistically enhanced TPA-induced GJIC inhibition (R: 24.90 +/- 13.50% vs. 35.82 +/- 17.18%, P < .05), further imposition of a noise MF abolished the synergistic effect of coherent MF (R: 32.51 +/- 18.37%). Overall, the present data clearly showed that although noise MF itself had no effect on GJIC of NIH3T3 cells, its superposition onto a coherent sinusoidal MF at the same intensity abolished MF-induced GJIC suppression. This is the first report showing that noise MF neutralizes 50 Hz MF-induced biological effect by using a signaling component as the test endpoint.  相似文献   

12.
We consider the possibility that DC magnetic fields can interact in a resonant manner with endogenous AC electric fields in biological systems. Intrinsic electric-field ion cyclotron resonance (ICR) interactions would be more physically credible than models based on external AC magnetic fields and might be expected as an evolutionary response to the long-term constancy of the geomagnetic field. Bioelectromagnetics 17:85–87, 1997. © 1997 Wiley-Liss, Inc.  相似文献   

13.
Short-circuit currents, surface electric fields, and axial current densities were measured in electrically grounded guinea pigs exposed to a uniform, vertical, ELF electric field. These data are 70–110% of corresponding values obtained in grounded rats exposed to the same electric field.  相似文献   

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测定在各种温度条件下和在ELF(极低频率)磁场作用下细胞生长周期分布谱的变化。实验结果表明温度不仅能使周期分布谱的离散性发生改变,而且也能使谱的峰值产生位移,而ELF磁场只能使周期分布谱的离散性发生变化,对谱的峰值没有显著影响。这一差异是由于温度和ELF磁场对细胞生长产生影响的机理不同。用计算模拟不同机理对细胞生长周期分布谱的影响与我们早先提出的机理相吻合,即温度影响细胞内各种生长因子、生物离子的  相似文献   

16.
The possibility of an association of early pregnancy loss (EPL) with residential exposure to ELF magnetic fields was investigated in a case-control study. Eighty-nine cases and 102 controls were obtained from the data of an earlier study aimed at investigating the occurrence of EPL in a group of women attempting to get pregnant. Magnetic-field exposure was characterized by measurements in residences. Strong magnetic fields were measured more often in case than in control residences. In an analysis based on fields measured at the front door, a cutoff score of 0.5 A/m (0.63 μT) resulted in an odds ratio of 5.1 (95% confidence interval 1.0–25). The results should be interpreted cautiously due to the small number of highly exposed subjects and other limitations of the data. © 1993 Wiley-Liss. Inc.  相似文献   

17.
Low-voltage electric fields were measured in conductive tissue culture media using three techniques: voltage slope, current density-conductivity, and dipole methods. All three methods tested yielded comparable results. However, all three techniques have associated errors. These errors fall into three major categories: those associated with the measurement equipment, those associated with electrodes, and errors in cross-sectional area measurements. Each source of error is discussed so that all can be taken into account during construction and/or testing of exposure equipment.  相似文献   

18.
    
To improve the assessment of magnetic field exposures for occupational health studies, the Multiwave® System III (MW3) was developed to capture personal exposures to the three‐dimensional magnetic field vector B (t) in the 0–3000 Hz band. To process hundreds of full‐shift MW3 measurements from epidemiologic studies, new computer programs were developed to calculate the magnetic field's physical properties and its interaction with biological systems through various mechanisms (magnetic induction, radical pair interactions, ion resonance, etc.). For automated calculations in the frequency domain, the software uses new algorithms that remove artifacts in the magnetic field's Fourier transform due to electronic noise and the person's motion through perturbations in the geomagnetic field from steel objects. These algorithms correctly removed the Fourier transform artifacts in 92% of samples and have improved the accuracy of frequency‐dependent metrics by as much as 3300%. The output of the MwBatch software is a matrix of 41 exposure metrics calculated for each 2/15 s sample combined with 8 summary metrics for the person's full‐period exposure, giving 294 summary‐exposure metrics for each person monitored. In addition, the MwVisualizer software graphically explores the magnetic field's vector trace, its component waveforms, and the metrics over time. The output was validated against spreadsheet calculations with pilot data. This software successfully analyzed full‐shift MW3 monitoring with 507 electric utility workers, comprising over 1 million vector waveforms. The software's output can be used to test hypotheses about magnetic field biology and disease with biophysical models and also assess compliance with exposure limits. Bioelectromagnetics 31:391–405, 2010. © 2010 Wiley‐Liss, Inc.  相似文献   

19.
测定在各种温度条件下和在ELF(极低频率)磁场作用下细胞生长周期分布谱的变化。实验结果表明温度不仅能使周期分布谱的离散性发生改变,而且也能使谱的峰值产生位移,而ELF磁场只能使周期分布谱的离散性发生变化,对谱的峰值没有显著影响。这一差异是由于温度和ELF磁场对细胞生长产生影响的机理不同。用计算机模拟不同机理对细胞生长周期分布谱的影响与我们早先提出的机理相吻合,即温度影响细胞内各种生长因子、生物离子的活性,而ELF磁场则可能通过对细胞膜的影响使细胞内的细胞生长必不可少的生物离子的浓度发生变化。  相似文献   

20.
    
Low frequency magnetic fields can influence biochemical reactions and consequently physiological rhythms and oscillations. To test this for a model reaction we used the chemical Belousov-Zhabotinsky (BZ) reaction, which is one of the simplest chemical oscillators. The oscillation frequency of the reaction was tracked optically by the absorption of blue light. Field treatment was carried out at room temperature in the middle of two Helmholtz coils. After starting the reaction, for 5 min the oscillations were monitored as control measurement, then during the next 10 min monitoring was with a magnetic field switched on, followed by a period of 5 min with the field switched off. A variety of exposure conditions have been tested: the frequency was varied between 5 and 1000 Hz, the field strength was varied up to 2.7 mT, different pulse shapes were used, the influence of the exposure temperature was tested, and the influence of the optimum exposure conditions (static magnetic field and the frequency of the dynamic field) as predicted by the ion parametric resonance (IPR) model has been measured. In conclusion, in no case any statistical significant influence of the magnetic treatment on the oscillation frequency of the BZ reaction could be detected (P > .05, t-test).  相似文献   

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