Factors Affecting the Abscission of Reproductive Organs in Yellow Lupins (Lupinus luteus L.): III. ENDOGENOUS GROWTH SUBSTANCES IN VIRUS-INFECTED AND HEALTHY PLANTS AND THEIR EFFECT ON ABSCISSION

Extracts of small and mature-size lupin pods yielded four substancesaffecting the growth of wheat-coleoptile sections: one acidpromotor (A), two acid inhibitors(B and X), and one neutralinhibitor(Y). Inhibitor B was extremely active, however, coleoptile sectionsshowed no signs of toxic effects; they resumed growth at a rapidrate after rinsing them and adding ß-indolylaceticand (IAA) to the medium. 1 µg of IAA was required to counteractthe effect of ‘B’ extracted from 230 mg. Of tissue.On an equal fresh weight basis the inhibiting action of ‘B’in lupin pods was 500–1,500 times more potent than thatof ‘inhibitor ß’ in etiolated pea seedlings. Small pods of plants infected with pea-mosaic virus yielded3 times the amount of ‘A’ of healthy plants (equivalentto 1 µg. IAA 0.3 µg. IAA per 25 g. of tissue respectively),and approximately the amount of ‘B’. Mature podsof virus-infected plants again yielded more‘A’,but also 2? times more ‘B’ than pods of healthyplants. Healthy pods yielded more ‘A’ than virus-infectedpods, and there was no difference in ‘X’. A lupin abscission test was developed and the effects of proximaland distal application of -naphthyl acetic acid (NAA) are presented,and discussed with respect to results of other abscission tests. ‘A’ accelerated abscission when applied proximally,and delayed or prevented it when applied distally. ‘B’strongly accelerated abscission when applied in either way.A possible mechanism explaining the abscission-inducing effectof developing pods on later flowers is discussed in terms ofthe substances ‘A’ and ‘B’. The partlyprevented abscission observed on virus-infected plants was foundto agree well with the proposed mechanism.     

Predator-prey interactions between Isochrysis galbana and Oxyrrhis marina. II. Release of non-protein amines and faeces during predation of Isochrysis

During the growth of Isochrysis galbana, several non-proteinamines may be detected in the growth medium. Of these, one (termed‘TTl’) accumulates in proportion to the numbersof cells present. The concentrations of ‘TTl’, andof another (termed ‘TA’), are 3–5 times higherin cultures in which Isochrysis is predated by Oxyrrhis marina.The lowest estimates of the concentration of extracellular ‘TTl’are an order of magnitude higher than those of any protein aminoacid. Of the protein amino acids, some like glycine are utilizedduring predation while others, like histidine, accumulate inthe medium Because of the unknown N-content and reactivity ofthe non-protein amines during HPLC, it is not possible to sayif these compounds (together with other components of dissolvedorganic N) form a significant proportion of the unaccountedfor N in the system after predatory activity. During predationin the absence of detectable free ammonium (when Isochrysismay be expected to be N-deprived), particles accumulate in themedium. Most of these are <2.5 µ.m in diameter andare suggested to be remains of digested prey. There is evidenceof a reassimilation of these particles by prey-deplete Oxyrrhis.     

The Influence of the Plant Growth-regulator, 2-Methyl-4-Chlorophenoxyacetic Acid, on the Metabolism of Carbohydrate, Nitrogen and Minerals in Solanum lycopersicum (Tomato)

The effects of the foliar application of phytocidal concentrationsof 2-methyl-4-chlorophenoxyacetic acid (MCPA) on change in totaldry weight, and in ‘available carbohydrate’ (starch,‘total’ and ‘reducing’ sugars), totalnitrogen, phosphorus, potassium, calcium, and magnesium of ‘tops’and roots of tomato plants have been followed over a periodof 14 days following spraying. There were two main treatments—‘nutrient’(nutrient supply to roots continued after spraying) and ‘water’(distilled water only supplied to roots after spraying) and‘water’ (distilled water only supplied to rootsafter spraying)—the sub-treatments consisting of ‘MCPA’versus ‘no-MCPA’ for each of the main treatments.Twelve different times of sampling were used. In analysing the present data, the quantity ‘residualdry weight’ (total dry weight less ‘available carbohydrate’),which was originally introduced by Mason and Maskell as a basisof reference for analyses of plant organs in short-period experimentsnot involving appreciable growth, has been used as an estimateof the permanent structure of plant growth. This new use ofthe ‘residual dry weight’ basis has brought outimportant features which were obscured when the data were leftin their primary form (as percentages of total dry weight oramounts per plant). Growth, as measured by increase in ‘residual dry weight’,was greatly inhibited by 2-methyl-4-chlorophenoxyacetic acidshortly after spraying, in both the presence and the absenceof nutrient. In the presence of 2-methyl-4-chlorophenoxyacetic acid, netassimilation rate (estimated as rate of increase in total dryweight per gram ‘residual dry weight’ of the ‘tops’)was greatly diminished while uptake of total nitrogen and ofP₂O₅ (estimated as increase in total nitrogen or of P₂O₅ ofthe whole plant per day per 1 g. ‘residual dry weight’of the roots) appeared to undergo a similar but much smallerdiminution. It seemed probable, however, that in the presenceof MCPA a larger proportion of the carbohydrate actually formedwas utilized for synthesis of aminoacids and protein. In the plant as a whole there was no evidence of actual depletionof ‘available carbohydrate’ as a result of MCPAtreatment, this fraction showing a steady increase in all treatmentsthroughout the experiment. The rate of increase was, however,much reduced by MCPA treatment. The ‘tops’ presentedmuch the same picture as the whole plant, but for the rootsthe situation was quite different. While the roots of the ‘no-MCPA’plants and also of the ‘MCPA-water’ plants showeda steady increase in available carbohydrate, those of the ‘MCPA-nutrient’plants rose only very slightly (from the initial value of 8mg. per plant to about 10 mg.) during the first 2 days, andthen in the next 2 days declined to a value (about 6 mg.) belowthe initial and remained at this low level for the rest of theexperiment. It is suggested that the phytocidal effect of 2-methyl-4-chlorophenoxyaceticacid in the presence of nutrient may be due to depletion ofthe ‘available carbohydrate’ supplies in the roots,which is shown to be brought about, in part, by reduced transportfrom the tops, and partly by the relatively greater utilizationof the carbohydrate present. These results offer an explanationfor the facts that plants showing vigorous growth are more easilykilled by MCPA and that perennial plants, particularly thosewith storage tissues in their roots, are more resistant. Further,they suggest the useful practical application that MCPA treatmentshould be given when the carbohydrate reserves of the rootsare at a minimum. For perennial plants, conditions might beexpected to be optimal for the application of MCPA in late spring,at a time when the first ‘flush’ of growth is slowingdown and before any appreciable new reserves of carbohydratehave been accumulated. It was also shown that 2-methyl-4-chlorophenoxyacetic acid preventedthe net synthesis of starch, but still permitted an appreciablenet formation of sucrose. 2-methyl-4-chlorophenoxyacetic acid appeared to have no effecton the uptake of potassium, calcium, or of magnesium. The lackof effect on potassium is contrasted with the previous observationby Rhodes, Templeman, and Thruston (1950) that sub-lethal concentrationsof MCPA, applied over a relatively long period to the rootsof tomato plants, specifically depressed the uptake of potassium.     

Improved confidence intervals for a difference of two cause-specific cumulative incidence functions estimated in the presence of competing risks and random censoring

A cause-specific cumulative incidence function (CIF) is the probability of failure from a specific cause as a function of time. In randomized trials, a difference of cause-specific CIFs (treatment minus control) represents a treatment effect. Cause-specific CIF in each intervention arm can be estimated based on the usual non-parametric Aalen–Johansen estimator which generalizes the Kaplan–Meier estimator of CIF in the presence of competing risks. Under random censoring, asymptotically valid Wald-type confidence intervals (CIs) for a difference of cause-specific CIFs at a specific time point can be constructed using one of the published variance estimators. Unfortunately, these intervals can suffer from substantial under-coverage when the outcome of interest is a rare event, as may be the case for example in the analysis of uncommon adverse events. We propose two new approximate interval estimators for a difference of cause-specific CIFs estimated in the presence of competing risks and random censoring. Theoretical analysis and simulations indicate that the new interval estimators are superior to the Wald CIs in the sense of avoiding substantial under-coverage with rare events, while being equivalent to the Wald CIs asymptotically. In the absence of censoring, one of the two proposed interval estimators reduces to the well-known Agresti–Caffo CI for a difference of two binomial parameters. The new methods can be easily implemented with any software package producing point and variance estimates for the Aalen–Johansen estimator, as illustrated in a real data example.     

Density distribution and size sorting in fish schools: an individual-based model

In fish schools the density varies per location and often individualsare sorted according to familiarity and/or body size. High densityis considered advantageous for protection against predatorsand this sorting is believed to be advantageous not only toavoid predators but also for finding food. In this paper, welist a number of mechanisms and we study, with the help of anindividual-based model of schooling agents, which spatial patternsmay result from them. In our model, schooling is regulated bythe following rules: avoiding those that are close by, aligningto those at intermediate distances, and moving towards othersfurther off. Regarding kinship/familiarity, we study patternsthat come about when agents actively choose to be close to relatedagents (i.e., ‘active sorting’). Regarding bodysize, we study what happens when agents merely differ in sizebut behave according to the usual schooling rules (‘sizedifference model’), when agents choose to be close tothose of similar size, and when small agents avoid larger ones(‘risk avoidance’). Several spatial configurationsresult: during ‘active sorting’ familiar agentsgroup together anywhere in the shoal, but agents of differentsize group concentrically, whereby the small agents occupy thecenter and the large ones the periphery (‘size differencemodel’ and ‘active sorting’). If small agentsavoid the risk of being close to large ones, however, smallagents end up at the periphery and large ones occupy the center(‘risk avoidance’). Spatial configurations are alsoinfluenced by the composition of the group, namely the percentageof agents of each type. Furthermore, schools are usually oblongand their density is always greatest near the front. We explainthe way in which these patterns emerge and indicate how resultsof our model may guide the study of spatial patterns in realanimals.     

Sieve Estimation for the Cox Model with Clustered Interval-Censored Failure Time Data

Clustered interval-censored failure time data occur when the failure times of interest are clustered into small groups and known only to lie in certain intervals. A number of methods have been proposed for regression analysis of clustered failure time data, but most of them apply only to clustered right-censored data. In this paper, a sieve estimation procedure is proposed for fitting a Cox frailty model to clustered interval-censored failure time data. In particular, a two-step algorithm for parameter estimation is developed and the asymptotic properties of the resulting sieve maximum likelihood estimators are established. The finite sample properties of the proposed estimators are investigated through a simulation study and the method is illustrated by the data arising from a lymphatic filariasis study.     

Evaluating the 'Labeled Magnitude Scale' for Measuring Sensations of Taste and Smell

The Labeled Magnitude Scale (LMS) is a semantic scale of perceptualintensity characterized by a quasi-logarithmic spacing of itsverbal labels. The LMS had previously been shown to yield psychophysicalfunctions equivalent to magnitude estimation (ME) when gustatory,thermal and nociceptive stimuli were presented and rated together,and the upper bound of the LMS was defined as the ‘strongestimaginable oral sensation’. The present study comparedthe LMS to ME within the more limited contexts of taste andsmell. In Experiment 1, subjects used both methods to rate eithertaste intensity produced by sucrose and NaCl or odor intensityproduced by acetic acid and phenyl ethyl alcohol, with the upperbound of the LMS defined as either the ‘strongest imaginabletaste’ or the ‘strongest imaginable odor’.The LMS produced psychophysical functions equivalent to thoseproduced by ME. In Experiment 2 a new group of subjects usedboth methods to rate the intensity of three different tastequalities, with the upper bound of the LMS defined as the ‘strongestimaginable [sweetness, saltiness, or bitterness]’. Inall three cases the LMS produced steeper functions than didME. Experiment 3 tested the hypothesis that the LMS yields datacomparable to ME only when the perceptual domain under studyincludes painful sensations. This hypothesis was supported whenthe LMS again produced steeper functions than ME after subjectshad been explicitly instructed to omit painful sensations (e.g.the ‘burn’ of hot peppers) from the concept of ‘strongestimaginable taste’. We conclude that the LMS can be usedto scale sensations of taste and smell when they are broadlydefined, but that it should be modified for use in scaling specifictaste (and probably odor) qualities. The implications of theseresults for theoretical issues related to ME, category-ratioscales and the size of the perceptual range in different sensorymodalities are discussed. Chem. Senses 21: 323–334, 1996.     

Semiparametric regression estimation in the presence of dependent censoring

We propose a semiparametric estimation procedure for estimatingthe regression of an outcome Y, measured at the end of a fixedfollow-up period, on baseline explanatory variables X, measuredprior to start of follow-up, in the presence of dependent censoringgiven X. The proposed estimators are consistent when the dataare ‘missing at random’ but not ‘missing completelyat random’ (Rubin, 1976), and do not require full specificationof the complete data likelihood. Specifically, we assume thatthe probability of censoring at time t is independent of theoutcome Y conditional on the recorded history up to t of a vectorof time-dependent covariates that are correlated with Y. Ourestimators can be used to adjust for dependent censoring andnonrandom noncompliance in randomised trials studying the effectof a treatment on the mean of a response variable of interest.Even with independent censoring, our methods allow the investigatorto increase efficiency by exploiting the correlation of theoutcome with a vector of time-dependent covariates.     

Growth and Maintenance of an Embryogenic Cell Culture of Daylily (Hemerocallis) on Hormone-free Medium

Callus cultures of the diploid daylily (Hemerocallis) clone‘Autumn Blaze’ were initiated and maintained inhormone-containing nutrient medium. At various times (from 6weeks to 1 year) after being initiated, hormone-derived cultureswere evaluated for their ability to be maintained and to multiplyon hormone-free medium at low pH (between pH 4 and 4.5). Cultureshad to be exposed to hormone-containing medium for at least12 weeks before they could be maintained on hormone-free mediumat low pH. The transition to maintainability on low pH hormone-freemedium included the production of many aberrant embryonal forms('neomorphs'). However, all hormone-derived cultures testedconsisted entirely of preglobular stage proembryos (PGSPs) after12–24 weeks on low pH hormone-free medium. PGSP cultureshave been maintained and multiplied as such for over 1 yearon low pH hormone-free medium. PGSPs continue their developmentinto various somatic embryo stages when cultured on hormone-freemedium buffered at pH 5.8. The production of well-formed somaticembryos was greatly enhanced when PGSPs were plated on activatedcharcoal impregnated filter papers that were placed on top ofthe agar surface. The gross morphology and histology of thePGSPs and stages of somatic embryo development are presented.The work shows that the ability of hormone-free medium at lowpH to permit PGSP multiplication without development into laterstages of embryo development is not restricted to carrot. Hemerocallis cv, ‘Autumn Blaze’, daylily, somatic embryogenesis, hormone-free medium, tissue culture     

Male attractiveness covaries with fighting ability but not with prior fight outcome in house crickets

In many species males that tend to win fights against othermales are more attractive to females. There are three ways inwhich male fighting ability and attractiveness may be associated:(1) attractiveness and fighting ability are influenced by thesame underlying traits (e.g., body size), (2) females prefermales that have directly observed winning fights, or (3) winningprevious fights indirectly improves a male's chance of beingpreferred by females. The last possibility may arise as a consequenceof the "loser effect"; in many species when a male loses a fighthis probability of losing subsequent fights increases. Thereare, however, no studies testing whether such a "loser effect"also influences male attractiveness. Here we show that maleattractiveness and fighting ability are positively correlatedin the house cricket, Acheta domesticus. Our experiment wasdesigned so that females could not directly observe the outcomeof fights, thus eliminating possibility (2) above. We then testedbetween possibilities (1) and (3) by making use of the factthat in some cricket species the "loser effect" can be eliminatedexperimentally by ‘shaking’ a male and stimulatingthe motor program for flying. We showed that in A. domesticus‘shaking’ does affect the outcome of subsequentfights. Males that had won two previous fights were less likelyto win a fight after being ‘shaken’ than when subjectto a control treatment. In contrast, males that had lost twoprevious fights were more likely to win a fight after being‘shaken’ than when they were not shaken. There was,however, no effect of ‘shaking’ on male attractiveness.We conclude that the "loser effect" does not alter the tendencyfor large, dominant males to be attractive to females. Instead,it appears that there are traits correlated with both fightingability and attractiveness. One such trait is body size. Fightwinners were significantly larger than losers and attractivenesswas positively correlated with male body size.     

Fitting semi-Markov models to interval-censored data with unknown initiation times

In a semi-Markov model, the hazard of making a transition between stages depends on the time spent in the current stage but is independent of time spent in other stages. If the initiation time (time of entry into the network) is not known for some persons and if transition time data are interval censored (i.e., if transition times are not known exactly but are known only to have occurred in some interval), then the length of time these persons spent in any stage is not known. We show how a semi-Markov model can still be fit to interval-censored data with missing initiation times. For the special case of models in which all persons enter the network at the same initial stage and proceed through the same succession of stages to a unique absorbing stage, we present discrete-time nonparametric maximum likelihood estimators of the waiting-time distributions for this type of data.     

Mycorrhizal Response of Two Tomato Genotypes Relates to their Ability to Acquire and Utilize Phosphorus

The purpose of this study was to determine how a plant's responseto colonization by mycorrhizal fungi relates to its abilityto acquire and utilize phosphorus for growth and reproduction.Two tomato genotypes previously found to be either responsive(‘LA1709’) or unresponsive (‘large cherry’)to mycorrhizal colonization during early vegetative growth wereexamined in detail. Plants were grown at four levels of addedphosphate or with mycorrhizal inoculum. Vegetative and reproductivegrowth, phosphorus uptake and root length density were measuredduring the course of plant development. Mycorrhizal symbiosissignificantly increased above-ground dry mass, root length,phosphorus content and yield under low phosphorus conditionsin ‘LA1709’, while it had less effect on these characteristicsin ‘large cherry’. When uninfected, however, ‘LA1709’grew and reproduced poorly unless high amounts of phosphoruswere added to the soil, while ‘large cherry’ grewwell under very low phosphorus conditions. This was because‘large cherry’ had significantly higher root lengthdensities than ‘LA1709’, enabling plants from thisgenotype to explore more soil volume and acquire greater amountsof phosphorus when grown without mycorrhizal fungi in low phosphorussoil. ‘Large cherry’ also had higher phosphorususe efficiency and allocated a greater proportion of phosphorusto reproduction when uninfected than ‘LA1709’. Itappears traits that affect a plant's ability to acquire andutilize phosphorus efficiently for growth and reproduction canalso affect its response to mycorrhizal colonization in tomato.Copyright1998 Annals of Botany Company. Tomato,Lycopersicon esculentum,mycorrhiza,Glomus etunicatum,phosphorus, reproduction, lifespan.     

Physiology of Flowering in Saccharum: I. DAYLENGTH CONTROL OF FLORAL INITIATION AND DEVELOPMENT IN S. SPONTANEUM L.

Flowering in two clones of Saccharum spontaneum L. is controlledby photoperiod. The earliest stages of development, ‘induction’and ‘initiation of the inflorescence axis primordium’(IAP) were optimally promoted under intermediate days of 12h 30 min, while the subsequent stage ‘initiation of inflorescencebranch primordia’ (IBP) was inhibited by days longer than13 h. The following stage ‘initiation of spikelet primordia’(ISP) showed a quantitatively intermediate response with anoptimum photoperiod of 9 h to 11 h. The elongation of the differentiatedinflorescence was found to be only slightly sensitive to photoperiodsof 13 h or longer in one of the clones. Unfavourable photoperiodsat stages following induction resulted in the arrest or delayof inflorescence development and when these were given duringthe IAP and IBP stages, reversion to the vegetative conditioncommonly occurred.     

Coastal eutrophication: recent developments in definitions and implications for monitoring strategies

The word ‘eutrophication’ has its root in two Greekwords: ‘eu’ which means ‘well’ and ‘trope’which means ‘nourishment’. The modern use of theword eutrophication is related to inputs and effects of nutrientsin aquatic systems. Despite a common understanding of its causesand effects, there is no agreed definition of coastal eutrophication.This communication aims to review recent developments in thedefinitions of coastal eutrophication, all of which focus on‘accelerated growth’, and to discuss the implicationsin relation to monitoring and assessment of ecological status.It is recommended that measurements of primary production, beinga sensitive and accurate indicator of eutrophication, shouldbe mandatory when monitoring and assessing the ecological statusof coastal waters. Written responses to this article should be submitted to KevinFlynn at k.j.flynn@swansea.ac.uk within two months of publication.For further information, please see the Editorial ‘Horizons’in Journal of Plankton Research, Volume 26, Number 3, Page 257.     

Developmental Genetics and Morphological Evolution of Flowering Plants, Especially Bladderworts (Utricularia): Fuzzy Arberian Morphology Complements Classical Morphology

This review compares new developmental models on flowering andother vascular plants with evolutionary hypotheses formulatedby Agnes Arber (1879–1960) and like-minded botanists.Special emphasis is laid on philosophical basics such as perspectivism,pluralism about evolutionary modelling, continuum way of thinking,and fuzzy logic. Arber's perspective is best labelled as F uzzyA rberian M orphology (FAM Approach). Its proponents (‘FAMmers’)treat structural categories (e.g. ‘roots’, ‘shoots’,‘stems’, ‘leaves’, ‘stipules’)in vascular plants as concepts with fuzzy borderlines allowingintermediates (including transitional forms, developmental mosaics).The FAM Approach complements Cla ssical Plant M orphology (ClaMApproach), which is the traditional approach in botany. ClaMproponents (‘ClaMmers’) postulate that the structuralcategories of vascular plants are regarded as concepts withclear-cut borderlines and without intermediates. However, duringthe evolution of vascular plants, the root-shoot distinctionand the stem-leaf distinction have become blurred several timesdue to developmental changes, resulting in organs with uniquecombinations of features. This happened, for example, in thebladderworts (Utricularia, Lentibulariaceae). When focusingon the ‘leaf’, the FAM Approach is identical toArber's ‘partial-shoot theory of the leaf’ and Sinha's‘leaf shoot continuum model’. A compound leaf canrepeat the developmental pathway of the whole shoot, at leastto some degree. For example, compound leaves of Chisocheton(Meliaceae)with indeterminate apical growth and three-dimensional branchingmay be seen as developmental mosaics sharing some growth processeswith whole shoots! We focus here on the FAM Approach becausethis perspective is especially promising for developmental geneticistsstudying flowering and other vascular plants. Copyright 2001Annals of Botany Company Review, body plan, developmental mosaics, leaf development, history of botany, homeosis, homeotic genes, Lentibulariaceae, morphological evolution, process morphology, stipules, Utricularia, flowering plants     

Dimorphism and Monomorphism in the Plumbaginaceae: II. Pollen and Stigmata In the Genus Limonium

A survey of pollen- and stigma-dimorphism and monomorphism inthe genus Limonium (as understood at present) reveals considerableheterogeneity which is useful for the solution of taxonomic,phylogenetic, and phytogeographic problems. Two main pollen types (A and B) and two main stigma types (coband papillate) occur in the genus. Capitate stigmata also occur. ‘Type A’ pollen has frequently been found in conjunctionwith ‘papillate’ stigmata as a secondarily monomorphic(self-compatible) combination and this must have arisen at leastfour times in the genus as well as in Armeria. ‘Type B’pollen and ‘cob’ stigmata are recorded togetherfor the first time (also as a secondarily monomorphic, self-compatiblecombination) in L. echioides. Apomixis, already discovered insubsections Densiflorae and Dissitiflorae, has been found inL. cosyrense of the subsection Steirocladae. The taxonomic and phylogenetic significance of the facts indiscussed.     

Effects of Inorganic Phosphate on the Utilization of Sucrose by Suspension-cultured Catharanthus roseus Cells

The metabolic fate of [U-¹⁴C]sucrose in suspension culturesof Catharanthus roseus cells was monitored for 96 h after thecells were transferred to fresh complete (‘+Pi’)or to phosphate-deficient Murashige and Skoog (‘–Pi’)medium. Sucrose was hydrolysed extracellularly to glucose andfructose. The rate of uptake of sugars by the cells was 1.5–3times higher in ‘+Pi’ culture than in ‘–Pi’culture. Little difference in the rate of incorporation of radioactivityinto the ethanol-soluble fraction was found between the ‘+Pi’and ‘– Pi’ cultures during the initial 24h of culture, but after 48 h the rate in ‘ +Pi’cultures was higher than that in ‘–Pi’ cultures.Incorporation of radioactivity into ethanol-insoluble macromoleculeswas always significantly higher in the cells in ‘+Pi’cultures than in those in ‘–Pi’ cultures.The results suggest that Pi strongly affects the utilizationof sugars by cultured plant cells through the stimulation oftransport of sugars as well as through the activation of metabolism. Catharanthus roseus, Madagascar periwinkle, suspension culture, inorganic phosphate, sucrose, transport, metabolism     

Plant Morphology: The Historic Concepts of Wilhelm Troll, Walter Zimmermann and Agnes Arber

Recent molecular systematic and developmental genetic findingshave drawn attention to plant morphology as a discipline dealingwith the phenotypic appearance of plant forms. However, sincedifferent terms and conceptual frameworks have evolved overa period of more than 200 years, it is reasonable to surveythe history of plant morphology; this is the first of two paperswith this aim. The present paper deals with the historic conceptsof Troll, Zimmermann and Arber, which are based on Goethe'smorphology. Included are contrasting views of ‘unity anddiversity’, ‘position and process’, and ‘morphologyand phylogeny’, which, in part, are basic views of currentplant morphology, phylogenetic systematics and developmentalgenetics. Wilhelm Troll established the ‘type concept’and the ‘principle of variable proportions’. Hehas provided the most comprehensive overview of the positionalrelations of plant forms. Agnes Arber started from the universaldynamics of life and attempted to describe all structures asprocesses. She paid attention to ‘repetitive branching’,‘differential growth’, and ‘parallelism’.As a result she has recently been rediscovered by developmentalbotanists. Walter Zimmermann rejected any metaphysical influenceon plant form and instead called for objective procedures. Hewas mainly interested in phylogenetic ‘character transformation’and the ‘reconstruction of genealogical lines’.Guided by the example of flower-like inflorescences, a futurepaper will deal with functional and developmental constraintsinfluencing plant forms. Recent morphological concepts (‘trialectical’,‘continuum’/‘fuzzy’, ‘processmorphology’) will be discussed and related to currentmorphological and developmental genetic research. Copyright2001 Annals of Botany Company Plant form, plant morphology, natural philosophy, homology, phylogeny, Goethe, Troll, Arber, Zimmermann, typology, character transformation, differential growth, complementarity     

Changes in the Pollen Grain Wall of Linum grandiflorum following Compatible and Incompatible Intraspecific Pollinations

The exine surrounding the colpal furrows in the pollen grainsof Linum grandiflorum is rapidly degraded following the twocompatible pollinations between ‘pin’ and ‘thrum’types of flower and also following the incompatible pollinationbetween ‘thrum’ and ‘thrum’. In allthese pollinations the pollen germinates normally. Exine isnot degraded in the incompatible pollination between ‘pin’and ‘pin’. In this pollination the pollen does notswell or germinate. The exine degradation is restricted to thecolpal regions. Hydration of the pollen grain by buffer solutionwithout contact with the stigma also stimulates the degradationof the exine. The erosion of the exine reveals a fibrous, PAS-positivematrix which follows the original contours of the exine. Vesicles are discharged into the intinc following pollination;these appear to contribute their contents to the intine-likewall which eventually invests the pollen tube. The observations are interpreted by assuming:

1. 1. an enzyme situatedwithin the pollen and localized underthe colpi; this enzymeis active after hydration;
2. 2. the degradation is a means bywhich colpal furrows are enlargedto permit the emergence ofthe pollen tube;
3. 3. the degradation process both by bufferand on the stigmais purely the consequence of hydration andis only secondarilyrelated to incompatibility through the ‘pin’by‘pin’ pollination not permitting hydration ofthepollen.

     

Theodoropoulos, D.I. Invasion biology. Critique of a pseudoscience.

The back cover of this book states that ‘contrary to theclaims of the nativists, research shows that man-dispersed speciesincrease biological diversity, benefit ecosystems, and act asan important force for healing the planet’. This is anuncompromising statement, and David Theodoropoulos divides hisdevelopment of the arguments supporting this statement intothree parts. Part I (Chapters 1–6) is ‘Nature, Dispersaland Reaction’. Part II (Chapters 7 and 8) is ‘Why?Psychology, Politics and Pseudoscience’. Part III (Chapters9–11) is ‘Humanity and Diversity’. There isalso an ‘Introduction’ including a summary of findingsand ‘An outline for a new theory of anthropogenic dispersal’,