毛喉鞘蕊花化学及生理活性研究进展

本文简要综述了唇形科鞘蕊花属植物毛喉鞘蕊花(Coleus forskohlii(wild．)Briq．)化学及药理研究进展,尤其是二萜成分的研究状况。     

毛喉鞘蕊花的化学成分

从毛喉鞘蕊花分离得到两个新二萜醌 :鞘蕊酮S(1) ,鞘蕊酮T(2 )和六个已知成分 :α 雪松醇 ,愈创木酚甘油醚 ,芫花素 ,鞘蕊醇B ,β 谷甾醇 ,豆甾醇。依据光谱和化学方法 ,它们的化学结构已被鉴定     

毛喉鞘蕊花的微量成分

从唇形科毛喉鞘蕊花全草的氯仿提取物分离到２个新的微量成分,鞘蕊花戊素和已素。基于光谱分析,鞘蕊花戊素和已 素的化学结构分别鉴定为１α,７β－二乙酰氧基－８,１３－环氧－６β羟基勒丹－１４烯－１１－酮（１）和７β－乙酰氧基－８,１３环氧－６β,α－二羟基勒丹－１４烯－１１－酮（２）。     

毛喉鞘蕊花愈伤组织的诱导及其活性成分的产生

本文简要报道了毛喉鞘蕊花(Coleus jorskohlii)无菌苗愈伤组织的诱导,初步培养及细胞悬浮培养,同时对培养细胞中的有效成分进行了初步分析。     

佛司可林类成分的光谱特征(一)

活性二萜佛司可林和异佛司可林已从毛喉鞘蕊花分离得到。本文详细描述了它们的光谱特征(包括一维和二维的核磁共振谱)。     

佛司可林类成分的光谱特征（二）

活性二萜佛司可林A、B、C、D,已从毛喉鞘蕊花分离得到。本文根据一维和二维的核磁共振谱详细描述了它们的光谱特征,改正了原先错误的指定。     

佛司可林类成分的光谱特征（三）

二萜佛司可林E、F、G、H,已从毛喉鞘蕊花分离得到。本文根据一维和二维的核磁共振谱详细描述了它们的光谱特征,改正了原先错误的指定。     

佛司可林类成分的光谱特征(四)

二萜佛司可林I、J、K、L,已从毛喉鞘蕊花分离得到。本文根据一维和二维的核磁共振谱详细描述了它们的光谱特征,二萜佛司可林K、L是首次报道。     

黄鞘蕊花中两个新的醌式二萜

从黄鞘蕊花中得到２个新的醌式二萜（二聚体）,黄鞘蕊花乙素,黄鞘蕊花丙素和２个已知化合物蒲公英萜醇,齐墩果酸。     

毛喉鞘蕊花的化学成分研究

本文报导了,从产于滇东北的毛喉鞘蕊花(Coleus forskohlii Brig)的根茎中分离到四个二萜化合物,经光谱分析鉴定,其结构分别为1α,6β,7α-triacetyl-coleonol—B(1),I-acetyl—forskolin(2),coleonol-B(3),和deacetyl-forskolin(4)。化合物(1)和(2)是首次从该植物中分离到。     

毛喉鞘蕊花毛状根培养及二萜化合物的形成

发根农杆菌ｐＲｉ１５８３４菌株感染毛喉鞘蕊花的根、茎、叶及小苗,均诱导出毛状根。通过纸电泳,在毛状根中检测到甘露碱。该毛状根不仅具有典型的形态特征,并能合成二萜化合物,对其中３个主要成分ｆｏｒｓｋｏｌｉｎ,１,９－ｄｉｄｅｏｘｙｆｏｒｓｋｏｌｉｎ和ｃｏｌｅｏｌ分别进行了定量测定。ＭＳ培养基有利于ｆｏｒｓｋｏｌｉｎ和ｃｏｌｅｏｌ的合成,而Ｂ５培养基促进１,９－ｄｉｄｅｏｘｙｆｏｒｓｋｏｌｉｎ的合成。     

In vitro propagation of Coleus forskohlii Briq. for forskolin synthesis

Shoot multiplication was obtained in vitro within 20–25 d from shoot tip expiants of 30 d old aseptically germinated seedlings of Coleus forskohlii Briq., using 2 mg/1 of 6 benzylaminopurine (BA). Shoot multiplication was further enhanced with the gradual decrease in the level of BA, and its final omission after 4 months. Different auxins supplemented at the level of 0.05 mg/1 with BA did not yield better results. Seven regenerated plants showed only diploid cells in their root tips, while three plants did not. Of these, two were diploid with occasional aneuploid cells. In one plant 32 chromosomes were observed. The potential of shoot culture in vitro and use of micropropagated plants for the production of forskolin has been demonstrated.     

Plant Probiotic Bacterial Endophyte,Alcaligenes faecalis,Modulates Plant Growth and Forskolin Biosynthesis in Coleus forskohlii

Probiotics and Antimicrobial Proteins - Coleus forskohlii is an herb, well-known for its medicinal compound forskolin present in its roots, with wide range of pharmaceutical applications. Here, we...     

Biotransformation of low-molecular-weight alcohols by Coleus forskohlii hairy root cultures

Coleus forskohlii hairy root cultures were shown to biotransform methanol and ethanol to the corresponding beta-D-glucopyranosides and beta-D-ribo-hex-3-ulopyranosides, and 2-propanol to its beta-D-glucopyranoside.     

Chonemorphine, stigmasterol, and ecdysterone: steroids isolated through bioassay-directed plant screening programs

In a bioassay-directed screening programme of plants for the identification of active constituents the following steroids were isolated. Chonemorphine was identified as the antiamoebic and antitrichomonad principle of Chonemorpha fragrans. Stigmasterol from Coleus forskohlii and ecdysterone from Diploclisia glaucescens were inactive constituents isolated during the process of purifying the active principles of the plants. D. glaucescens roots are a high-yielding source (0.5% of the dry root weight) of ecdysterone.     

印产毛喉鞘蕊花的引种栽培研究

本文报道印产毛喉鞘蕊花肉质根稳定性状的分离及栽培技术路线．肉质根株提纯到９９．５％;求得了露地最佳效益种植法,最优水平搭配是：３０ｄ、１５株／ｍ２、高垄、覆膜、底肥、整枝、去花穗,产鲜根８７００ｋｇ／ｈｍ２．     

Potential application of urea-derived herbicides as cytokinins in plant tissue culture

Various urea-derived herbicides and different cytokinin analogues were used to determine their effects on callusing response and shoot regenerating capacity of alfalfa (Medicago sativa L.) and Coleus (Coleus forskohlii Briq.). The herbicides monuron and diuron evoked profuse callusing response from Coleus leaf segments and alfalfa petiole explants on Murashige and Skoog medium. Shoot regeneration by monuron (2.0 mg/l) showed a maximum of 3 multiple shoots both in alfalfa and Coleus with a frequency of 92% and 75%, respectively. Whereas diuron (0.5 mg/l) showed a high frequency of shoot regeneration (89%) with a mean number of 5 shoots in alfalfa, inC. forskohlii, the frequency of regeneration was 90% with a mean number of 6 shoots. Diuron with two chloride groups in the phenyl ring showed significantly higher cytokinin-like activity than single chloride substitution monuron. This study demonstrates the potential use of monuron and diuron as cytokinins in plant tissue culture.