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关于体外培养周期型马来丝虫(periodic Brugia malayi)感染期幼虫(L_3),近年来国内外有一些报道,系应用含哺乳动物组织细胞系或原代细胞的培养系统培养彭亨丝虫和马来丝虫L_3,可使其幼虫发育至Ⅳ期蚴(L-4)或早期成虫(Chen and Howells,1979; Wong et al.,1982;Mak et al.,1983;郑惠君等,1984)。但未见应用含人体组织细胞系的体外培养系统培养布鲁属丝虫L_3的报道。本研究 相似文献
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液氮冻存丝虫微丝蚴的研究,国外有盘尾属丝虫、盂欧氏丝虫、斑氏丝虫、马来丝虫和犬恶丝虫等,国内尚未见有报道。本试验应用三种低温保护剂,对两种丝虫微丝蚴进行液氮低温保存的研究,获得初步成功,现简报如下: 相似文献
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根据山东长清县张夏镇馒头山馒头组采集到的三叶虫新材料,重新厘定和恢复群星虫(Asteromajia Nan and Chang,1982)作为裂头虫科内有效属名的地位,将徐庄虫(Hsuchuangia Lu and Zhu in Qiu et al.,1983),八公山虫(Bagongshania Lin in Qiu et al.,1983)作为群星虫的晚出异名。修订我国以往所描述的毛庄期和徐庄期4个属(Kochaspis Resser,1935,Asteromajia Nan and Chang,1982,Hsuchuangia Lu and Zhu in Qiu et al.,1983,Bagongshania Lin in Qiu et al.,1983)内的15个种的特征和头尾搭配,归并为4属,10种:Temnoura huoshanensis(Zhang and Wang,1985),Zhongtiaoshanaspis angustilimbata(Meng in Zhou et al.,1977),Solenoparia funingensis(An in Duan et al.,2005),Asteromajia hsuchuangensis(Lu in Lu and Dong,1952),A.quadrata(Resser and Endo,1937),A.liuheensis(An,1966),A.huainanensis(Lin in Qiu et al.,1983),A.lüliangshanensis(Zhang and Wang,1985),A.?yangchengensis(Zhang and Wang,1985),A.?longiceps(Lu and Zhu,2001),其中后2个种的归属尚有疑问。 相似文献
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根据山东长清县张夏镇馒头山馒头组采集到的三叶虫新材料,重新厘定和恢复群星虫(Asteromajia Nan and Chang,1982)作为裂头虫科内有效属名的地位,将徐庄虫(Hsuchuangia Lu and Zhu in Qiu et al.,1983),八公山虫(Bagongshania Lin in Qiu et al.,1983)作为群星虫的晚出异名。修订我国以往所描述的毛庄期和徐庄期4个属(Kochaspis Resser,1935,Asteromajia Nan and Chang,1982,Hsuchuangia Lu and Zhu in Qiu et al.,1983,Bagongshania Lin in Qiu et al.,1983)内的15个种的特征和头尾搭配,归并为4属,10种:Temnoura huoshanensis(Zhang and Wang,1985),Zhongtiaoshanaspis angustilimbata(Meng in Zhou et al.,1977),Solenoparia funingensis(An in Duan et al.,2005),Asteromajia hsuchuangensis(Lu in Lu and Dong,1952),A.quadrata(Resser and Endo,1937),A.liuheensis(An,1966),A.huainanensis(Lin in Qiu et al.,1983),A.lüliangshanensis(Zhang and Wang,1985),A.?yangchengensis(Zhang and Wang,1985),A.?longiceps(Lu and Zhu,2001),其中后2个种的归属尚有疑问。 相似文献
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<正>Dear Editor,Bisphenol A (BPA), a common and widely used monomer in the production of polycarbonate plastics and epoxy resin,(Healy et al., 2015; De Filippis et al., 2018) has been defined as an endocrine disrupting chemical (EDC) associated with the etiology of insulin resistance (IR)(Gioiosa et al., 2015;Wei et al., 2017) and metabolic disorders (Padmanabhan et al., 2010; Fang et al., 2015; Balistrieri et al., 2018). However, 相似文献
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《遗传学报》2016,(7)
正Ribosomes are large RNA and protein complexes that function as the machinery for translation protein synthesis(Boisvert et al.,2007;Ben-Shem et al.,2011;Henras et al.,2015;Khatter et al.,2015;McCann et al.,2015).The eukaryotic ribosome is composed of two subunits,the 60S large subunit(LSU)and the 40S small subunit(SSU),which collectively comprise of four different ribosomal RNA(rRNA)species and more than 70 proteins(Ben-Shem et al.,2011;Henras et al.,2015;Khatter et al.,2015).The LSU contains 相似文献
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外周血液中的微丝蚴一般都有一定的昼夜周期和季节周期,然而皮肤组织中微丝蚴的周期性都不明显。在盘尾丝虫属中,据Mellor对马皮肤中(Onchocerca cervicalis)微丝蚴观察和Eichler对牛皮组织中(Onchocerca gutturosa)微丝蚴测定以及Marroquin(1974);Auderso(1975)对人体皮肤中(Onchocerca volvulus)微丝蚴的研究,都表明无周期节律,本丝虫的周期性尚无详细了解,特别是在中国还缺乏详细研究。我们于1984年2月至1985年 相似文献
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<正>Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al."2004;Liu and Chen,2007;Reddy et al.,2008;Wang 相似文献
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《遗传学报》2020,47(6):333-336
正Nonhuman primates (NHPs) present significant advantages over rodents for physiological and pharmacological investigations because of their higher genetic and physiological similarity to humans. Both rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) monkeys are considered ideal animal models in biomedical research (Phillips et al., 2014), especially in modeling normal development and diseases in the nervous system (Izpisua Belmonte et al., 2015; Ng et al., 2015; Niu et al., 2015; Poo et al., 相似文献
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正In the last decade, cotton genomics had great progress in genome assembling(Li et al., 2015; Huang et al., 2020) and agriculturally important locus identification(Du et al., 2018;Ma et al., 2018; Li et al., 2021).Upland cotton(Gossypium hirsutum) is one of the most important cash crops cultivated worldwide. The fiber quality of cotton directly determines its economic value, and therefore enhancing fiber quality is always the primary task in cotton breeding practices(Xia et al., 2019; Huang et al.,2021). 相似文献
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Xinliang Fu Lifang Wang Bo Fang Ruirui Ma Yun Zheng San Huang Pei Zhou Zongxi Cao Jin Tian Shoujun Li Guihong Zhang 《中国病毒学》2016,31(5):454-456
正Several recent studies in Virologica Sinica and other journals have highlighted the enormous international challenge of emerging arboviral diseases,such as Zika virus disease,dengue,and chikungunya(Islam et al.,2015;Maurice et al.,2015;Dai et al.,2016;Deng et al.,2016;Song et al.,2016;Wang et al.,2016;Xu et al.,2016;Zhang et al.,2016;Zhou et 相似文献
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Lunzhi Yuan Huachen Zhu Ming Zhou Jian Ma Rirong Chen Liuqin Yu Wenjia Chen Wenshan Hong Jia Wang Yao Chen Kun Wu Wangheng Hou Yali Zhang Shengxiang Ge Yixin Chen Quan Yuan Qiyi Tang Tong Cheng Yi Guan Ningshao Xia 《蛋白质与细胞》2022,13(1):72
Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has infected more than 200 million people,causing coronavirus disease 2019(COVID-19)worldwide.Lungs are the primary target organ of SARS-CoV-2 infection.The mild COVID-19 cases develop symptoms of fever,fatigue,muscle weakness,chest pain,headache and cough(Chen et al.,2020;Wang et al.,2020;Zhu et al.,2020),while severe COIVD-19 cases might have pneumonia,breathing difficulties,multiple organ failure and death(Chen et al.,2020;Wang et al.,2020;Zhu et al.,2020).Both the clinicians and researchers have largely focused on the acute phase of COVID-19,but the long-term health consequences of the COVID-19 patients after clinical recovery remain less investigated.Several clinical cohorts demonstrated that some patients discharged from hospital still experience symptoms including fatigue,muscle weakness,chest pain,cough and breathing difficulties(Carfi et al.,2020;Lim et al.,2020;Huang et al.,2021).Moreover,severely impaired pulmonary diffusion capacities and abnormal chest imaging manifestations were observed in some convalescent patients(Huang et al.,2021).Surprisingly,residual virus(An et al.,2020;Yao et al.,2020;Kim et al.,2021)were detected in some convalescent patients. 相似文献
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近年来有关多糖抑瘤作用与结构的关系已有不少报道(Chihara,1970;Ueno et al.,1982;Miya-zaki et al.,1979;Misaki et al.,1981;Inoue et al.,1983;张翼伸、李冶平,1981),但尚无严格的规律可循。不同的多糖结构,不同的瘤株,剂量大小,给药途径等都有不同的效应。为进一步探讨多糖抑瘤与结构的关系,作者以密簇斜盖伞菌(Clitopilus caespitosus)中提取的两种多糖及其化学修饰物为材料,对它们的抑瘤活性进行了比较研究,现将结果报道如下。 相似文献
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采用人工感染法,对严重联合免疫缺陷小鼠(Scid 小鼠) 进行夜现周期性斑氏丝虫实验感染研究。结果表明,斑氏丝虫经人工感染后能在Scid 小鼠体内发育成熟并产生微丝蚴,且虫体回收率较高为6~30% ,平均14-8% 。感染的10 只小鼠均在外周血中检获出微丝蚴,及解剖收集到成虫,感染成功率高达100% 。作者认为Scid 小鼠由于T、B淋巴细胞功能缺如,不能产生相应的抗丝虫感染抗体,是一种易于感染成功、潜伏期短、微丝蚴密度及成虫回收率高的较理想的实验动物。 相似文献
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Yuxian Zhu 《中国科学:生命科学英文版》2016,59(2):109-111
正As one of the important industrial crops and also an excellent model organism for studying cell elongation,cell wall biosynthesis as well as polyploidy evolution,cotton has always been in the limelight of the world(Shi et al.,2006;Qin and Zhu,2011).With the publication of the allotetraploid Gossypium hirsutum and Gossypium barbadense genomes this year(Li et al.,2015;Zhang et al.,2015;Liu et al.,2015),all three important cotton genomes,including the two ancestor diploid Gossypium raimondii(DD)(Wang et al.,2012;Paterson et al.,2012)and Gossypium arboreum(AA)(Li et al.,2014)have been successfully sequenced by scientists from China and from the United States of America.Since functional studies of Arabidopsis thaliana and 相似文献
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<正>哺乳动物的尿液挥发性成分被认为是同哺乳动物信息激素一样重要的物质,迄今报道的野生动物尿液挥发性成分研究的有狮(Panthera leo)(Andersen and Vulpius,1999)、山猫(Lynx rufus)(Mattina et al.,1991)、土狼(Canis lupus)(Raymer et al.,1986)、郊狼(Canis latrans)(Schultz,1988)、红狐(Vulpes vulpe)(Jorgensen et al.,1978)以及鼬科物种(Zhang et al.,2005)。Robert和Joseph(1991)利用山猫和土狼尿液气味抑制白尾鹿(Odocoileus leucurus)对日本 相似文献
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本实验用长爪沙鼠体内周期型马来丝虫微丝蚴,感染东乡伊蚊收集感染期幼虫,经腹腔内注射55只小白鼠(昆明种)后,定期抽查小白鼠腹腔液和解剖小白鼠观察丝虫发育情况。在14天、30天、60天、140天和160天时,分别检获了Ⅲ期、Ⅳ期幼虫、童虫和成虫。成虫感染率为34.6%(9/26)。91天还查出微丝蚴。实验结果表明:周期型马来丝虫可以在昆明种小白鼠体内发育到成虫并产生微丝蚴。 相似文献