共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
Abstract The effects of delayed mating on the copulation duration, female fertility, fecundity, egg fertility, longevity and the number days alive after mating of females of diamondback moth (DBM), Plutella xylostella, were studied. When male mating was delayed, the female fertility, fecundity, egg fertility, longevity and number days alive after mating of DBM decreased, and there was a negative correlation between the age of the moth with those variables except copulation duration. When female mating was delayed, the female fertility, fecundity, percent egg fertility and number days alive after mating of DBM also decreased, but the longevity increased, which also showed a negative relationship between the age of the moth with the variables except copulation duration and longevity. When both males and females delayed mating, the female fertility and fecundity decreased; egg fertility was affected marginally, and the longevity of females increased. The moth age was negatively correlated with those variables. 相似文献
3.
利用RNAi技术沉默小菜蛾类钙粘蛋白基因 总被引:6,自引:0,他引:6
RNA干扰(RNA interference, RNAi)是一种调控基因表达的方法, 其通过体外合成一段与内源靶基因同源的双链RNA(dsRNA)或siRNA, 导入生物体内, 使内源靶基因中同源mRNA降解, 从而达到阻抑基因表达的目的。类钙粘蛋白(cadherin-like protein)是位于昆虫中肠刷状缘膜囊(brush border membrane vesicles, BBMV)上与钙粘蛋白(cadherin)结构相似的物质, 是多种昆虫体内Bt杀虫蛋白的受体。本研究利用基因特异引物通过RT-PCR扩增了小菜蛾类钙粘蛋白基因的2个片段(CAD1和CAD2), 合成相对应的双链RNA(double-stranded RNA, dsRNA); 并将dsRNA通过显微注射导入小菜蛾3龄幼虫体内, 测定了不同靶位点、不同剂量、不同检测时间对目的基因mRNA表达量的影响。结果表明: 将70 nL CAD1对应的dsRNA注射到幼虫体内48 h后, 基因表达量显著下降, 72 h后恢复。免疫印迹检测结果表明, 类钙粘蛋白在注射dsRNA 48 h后幼虫BBMV中的含量明显下降。本实验成功实现了小菜蛾类钙粘蛋白基因的沉默, 该体系的成功建立为利用RNAi技术分析小菜蛾及其他鳞翅目昆虫基因的功能提供了参考。 相似文献
4.
Sublethal effects of hexaflumuron on development and reproduction of the diamondback moth,Plutella xylostella (Lepidoptera: Yponomeutidae) 总被引:1,自引:0,他引:1
Mohammad Mahmoudvand Habib Abbasipour Aziz Sheikhi Garjan Ali Reza Bandani 《Insect Science》2011,18(6):689-696
Abstract Effects of hexaflumuron at 10% lethal concentration (LC10) and LC25 on development and reproduction parameters of the diamondback moth, Plutella xylostella (Linnaeus, 1753) (Lep.: Yponomeutidae) were investigated. Estimated LC50, LC10 and LC25 values of leaf dip bioassay of hexaflumuron on the third instar larvae of the P. xylostella were 1.48, 0.59 and 0.91 mg/L, respectively. Hexaflumuron decreased pupal weight in the parent generation at sublethal concentrations but in the offspring generation, this effect was not observed. Sublethal concentrations increased egg, first and second larval instar and pupa developmental time and shortened life span of adults, but did not change the third and fourth larval instars and pre‐pupa developmental period. Also fecundity of females reduced significantly but hatchability of treatments and control were similar. Survival rate of pre‐adult stages declined significantly at LC25 concentration. Reproduction parameters such as reproductive rate (R0) and intrinsic rate of increase in sublethal concentrations were significantly lower compared with control, but gross reproduction rate (GRR) at the LC10 concentration was increased and it could be hormoligosis. Also hexaflumuron significantly increased doubling time (Dt). We conclude that the sublethal effects of hexaflumuron might exhibit significant effects on the population dynamics of P. xylostella. 相似文献
5.
Mohammad Mahmoudvand Habib Abbasipour Aziz Sheikhi Garjan Ali Reza Bandani 《Applied Entomology and Zoology》2011,46(1):75-80
The effects of sublethal concentrations of indoxacarb on Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) were examined. In comparison with the control, sublethal concentrations led to a significant
decrease in adult emergence, the percentage of pupation and pupal weight of the parent generation, fecundity, and adult longevity.
They also significantly decreased the net reproductive rate (R
0), intrinsic rate of increase (r
m), finite rate of increase (λ), and gross reproduction rate (GRR), and had significantly increased egg developmental time
and doubling time (Dt) of P. xylostella. However, sublethal concentrations of indoxacarb had no significant affect on adult emergence, percentage of pupation, larva,
prepupa and pupa developmental periods, hatchability, and sex ratio of offspring. The results of this study indicated that
sublethal concentrations can decrease the biological parameters of P. xylostella. 相似文献
6.
【目的】小菜蛾是危害十字花科蔬菜的世界性重要害虫。通过分析抗生素对小菜蛾的毒性效应,了解肠道细菌对小菜蛾适合度的影响,有助于更好地阐明小菜蛾肠道细菌的功能。【方法】利用抗生素处理含有高丰度肠道细菌的萝卜苗饲养品系(FZss)小菜蛾幼虫,同时利用抗生素处理饲料饲养的无肠道细菌(SLss)小菜蛾幼虫,分析抗生素及肠道细菌对小菜蛾适合度的影响。【结果】抗生素处理FZss品系小菜蛾导致了小菜蛾发育历期延长,虫重、蛹重、化蛹率、产卵量和成虫寿命降低。利用抗生素处理无肠道菌的SLss品系小菜蛾幼虫,小菜蛾化蛹率和单雌产卵量均显著降低,而对发育历期、虫重和蛹重则无影响。【结论】综合两个研究的结果发现抗生素处理后宿主适合度的降低一方面是由于抗生素的毒性效应导致,另一方面是由于小菜蛾肠道细菌的缺失引起。抗生素的毒性效应主要表现为对化蛹率和单雌产卵量的影响,而肠道细菌则对小菜蛾的发育历期、虫重、蛹重以及成虫寿命具有重要的促进作用。 相似文献
7.
8.
W. Wei T. H. Schuler S. J. Clark C. N. Stewart Jr & G. M. Poppy 《Journal of Applied Entomology》2008,132(1):1-11
The movement of Bacillus thuringiensis (Berliner) (Bt) Cry1Ac endotoxin through high trophic levels was assessed to help elucidate the effects of Bt toxin on non‐target insects. The diamondback moth (Plutella xylostella L., Lepidoptera: Plutellidae), the parasitic wasp (Cotesia vestalis Haliday, Hymenoptera: Braconidae) and the predatory green lacewing Chrysoperla carnea (Stephen) (Neuroptera: Chrysopidae) were used as a model system in this laboratory study. Bt‐resistant P. xylostella larvae fed Cry1Ac‐expressing transgenic oilseed rape (OSR, Brassica napus L., Cruciferae), before and after parasitization by C. vestalis, consumed Cry1Ac with the ingested plant material but only a proportion of Cry1Ac consumed was recovered from the bodies and faeces of P. xylostella larvae. Cry1Ac was not detected in newly emerged parasitoid larvae. In contrast, Cry1Ac was detected in C. carnea larvae fed on resistant P. xylostella larvae reared on Bt OSR. However, no Cry1Ac could be detected in C. carnea larvae when the lacewings were transferred to P. xylostella larvae reared on conventional OSR and tested 24–48 h. The metabolizing ability of Cry1Ac is discussed for the larvae of P. xylostella and C. carnea. 相似文献
9.
1. Whether the potential fecundity of herbivorous insects is realised or not may depend on female behaviour, which in turn may be influenced by host plant acceptability. Female Bupalus piniarius were observed to discriminate against needles growing out the year following defoliation (current‐year needles) of its host plant Pinus sylvestris. 2. It was hypothesised that the discriminatory behaviour was due to current‐year needles being less secure as a substrate. Field and laboratory experiments were designed to test this hypothesis and to estimate the discrepancy between potential and realised fecundity when females were offered defoliated branches. 3. In a laboratory oviposition experiment, B. piniarius females were exposed to branches bearing either current‐year needles only or both mature and current‐year needles. Daily oviposition rate, egg batch size, longevity, and mature eggs and fat retained at death were recorded for each female. In field experiments, the rate at which eggs dropped from expanding needles and the capacity of neonate larvae hatching from the dropped eggs to colonise a tree were assessed. 4. Significantly fewer eggs were laid when females were exposed to defoliated branches. 5. Twenty‐six and 16% of the eggs laid on current‐year needles dropped from the needles in 1998 and 1999 respectively, whereas no eggs dropped from mature needles in 1998 and only one egg (< 1%) dropped in 1999. 6. A very small proportion of larvae hatching on the forest floor (simulated egg drop) was able to recolonise host trees. 7. These results emphasise the importance of oviposition behaviour on realised fecundity when analysing insect population dynamics. In the case of B. piniarius, egg placement, although a minor detail during the normal course of events, became of key importance when defoliation deprived females of their preferred egg attachment site. 相似文献
10.
Interaction between insect strain and artificial diet in diamondback moth development and reproduction 总被引:3,自引:0,他引:3
The economical production of physiologically and behaviorally competent diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is critical to most research and control programs against this insect. Although a few laboratory-adapted colonies are currently maintained on artificial diets, the establishment and adaptation of feral DBM onto semi-synthetic diets is often difficult. Understanding the interactions between insect strain and diet may be critical to the process of laboratory adaptation as well as to the successful use of laboratory-reared insects in the field. As such, the objective of this study was to investigate the interaction between several DBM colonies/strains and different natural and semi-synthetic diets. Specifically, we examined the effect of different diets on the length of development, percent survival, adult weight, female fecundity, and adult longevity for two feral and one laboratory-adapted strain of DBM. Significant interactions were observed between diet and laboratory-adapted and feral strains, and also between diet and different feral strains with respect to many of the growth and development parameters tested. Therefore, the performance of one strain of DBM on a particular diet was not necessarily predicted by the performance of another DBM strain on the same diet. However, the soy-based diet developed and reported herein performed well for all three DBM strains tested. In future efforts to colonize feral DBM, we suggest that researchers assay different diet formulations in order to identify a semi-synthetic diet that is most suitable for the particular DBM strain under consideration. 相似文献
11.
Qi G Lu J Zhang P Li J Zhu F Chen J Liu Y Yu Z Zhao X 《Journal of applied microbiology》2011,111(5):1224-1234
Aims: Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long‐horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long‐horned beetle. Methods and Results: A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ‐89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ‐89 to beetle was 55·33%. Additionally, the body weight and egg‐hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ‐89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ‐89 crystal protein was purified and analysed by peptide‐mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS‐PAGE analysis showed the BMB171‐Cry1Ac89 recombinant strain successfully expresses a 133‐kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults. Conclusions: The strain of ZQ‐89 is highly toxic to the adults of long‐horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment‐friendly bioinsecticides against long‐horned beetles such as B. horsfieldi in the future. Significance and Impact of the Study: It is the first report of a B. thuringiensis strain toxic to the adults of Asian long‐horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests. 相似文献
12.
小菜蛾对Bt毒素Cry1Ac和Bt制剂抗性的选育及其抗性种群的生物学适应性 总被引:11,自引:0,他引:11
用Bt制剂和Bt毒素Cry1Ac分别对源自深圳田间的小菜蛾Plutella xylostella在室内进行抗性种群选育,获得相对抗性倍数分别为24.36、38.16倍的抗性种群DBM.1Ac-R30和DBM.Bt-R46。对这2个抗性种群及其敏感种群(DBM.Bt-S)的生长发育、存活及繁殖特征进行了详细地观察与比较,并以甘蓝为饲喂材料构建了2个抗性实验种群的生命表。结果表明,DBM.1Ac-R30和DBM.Bt-R46种群较DBM.Bt-S种群的产卵量和孵化率下降,幼虫发育历期延长,雌雄比显著降低,雌成虫数量、寿命减少。DBM.1Ac-R30和DBM.Bt-R46种群相对于DBM.Bt-S种群的相对适合度分别为0.75和0.65,抗性种群在繁殖能力上存在明显的生存劣势。 相似文献
13.
不同龄期幼虫饥饿对美国白蛾Hyphantria cunea(Drury)生长发育和繁殖影响的研究结果表明:美国白蛾低龄(2龄)幼虫短时间的饥饿对其生长发育和繁殖的影响不明显;美国白蛾中龄(4龄)幼虫饥饿2,4,6d使美国白蛾的历期相应增长,存活率、化蛹率、羽化率、产卵量都相应降低,交配率与对照之间没有差异;老熟(6龄)幼虫短时间饥饿(4d)的存活率、羽化率、交配率、产卵量都稍有下降;长时间饥饿(12d)的老熟幼虫有70%左右因饥饿而提前化蛹,提前化蛹的蛹体外没有薄茧的包裹。提前化蛹的美国白蛾的羽化率、交配率均非常低,产卵量很少。 相似文献
14.
Rana M. SARFRAZ Lloyd M. DOSDALL Andrew B. KEDDIE Judith H. MYERS 《Entomological Science》2011,14(1):20-30
The diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an important pest of cultivated brassicaceous crops worldwide. The host plant preferences, developmental biology and survival and longevity of P. xylostella are relatively well understood on commercial crop species; however, its relationship with brassicaceous weeds is poorly known. Sinapis arvensis L., Erysimum cheiranthoides L. and Capsella bursa‐pastoris (L.) Medicus are among the most common brassicaceous weeds worldwide and can serve as important bridge hosts of P. xylostella. In this study, preference and performance of P. xylostella were compared on these weed species. In free‐choice situations, females deposited 5.5 and 18.8 times more eggs on S. arvensis than on E. cheiranthoides and C. bursa‐pastoris, respectively. Survival from neonate to pupa and from pupa to adult was highest on S. arvensis and E. cheiranthoides and lowest on C. bursa‐pastoris. Development was fastest, foliage consumption was greatest, pupae and silk cocoons were heaviest, adult body masses and longevities were highest and forewings were largest for both females and males when reared as larvae on S. arvensis. Realized fecundity of new generation adults was highest for individuals reared on S. arvensis compared to those reared on E. cheiranthoides or C. bursa‐pastoris. Relative growth rates of pupae and adults were highest on S. arvensis, suggesting that this plant species is a high‐quality host for P. xylostella compared with other species tested. Potential impacts of these wild brassicaceous species on P. xylostella populations are discussed. 相似文献
15.
16.
17.
Experience-induced preference for oviposition repellents derived from a non-host plant by a specialist herbivore 总被引:4,自引:0,他引:4
Foraging adults of phytophagous insects are attracted by host‐plant volatiles and supposedly repelled by volatiles from non‐host plants. In behavioural control of pest insects, chemicals derived from non‐host plants applied to crops are expected to repel searching adults and thereby reduce egg laying. How experience by searching adults of non‐host volatiles affects their subsequent searching and oviposition behaviour has been rarely tested. In laboratory experiments, we examined the effect of experience of a non‐host‐plant extract on the oviposition behaviour of the diamondback moth (DBM), Plutella xylostella, a specialist herbivore of cruciferous plants. Naive ovipositing DBM females were repelled by an extract of dried leaves of Chrysanthemum morifolium, a non‐host plant of DBM, but experienced females were not repelled. Instead they were attracted by host plants treated with the non‐host‐plant extract and laid a higher proportion of eggs on treated than on untreated host plants. Such behavioural changes induced by experience could lead to host‐plant range expansion in phytophagous insects and play an important role in determining outcome for pest management of some behavioural manipulation methods. 相似文献
18.
The diamondback moth (DBM), Plutella xylostella (L.), is a major pest of cruciferous crops. Tebufenozide, a novel nonsteroidal ecdysone agonist, exhibits good efficacy and has played an increasingly important role in the control of Lepidopteran pests in China. For its resistance management, the genetic basis of tebufenozide resistance was studied using a laboratory selected resistant strain of DBM (resistant ratio, RR = 268). A series of crosses with laboratory susceptible and resistant strains revealed that tebufenozide resistance in this pest was partially biased toward female heredity, with a large difference in RR for F1 (RR = 29) and rF1 progeny (RR = 147). The dominance calculated for these 2 cross progeny was ?0.788 and 0.09, respectively. Further analysis showed that the susceptible male and female larvae were similar in their sensitivity to tebufenozide, but the resistant female larvae showed significantly higher resistance than the resistant male larvae. The heredity of tebufenozide resistance in DBM might be linked with the W sex chromosome, which suggested that DBM has the ability to develop high levels of resistance to tebufenozide. This is the first report of sex‐linked inheritance of tebufenozide resistance in P. xylostella (L.). 相似文献
19.
Gilmar da Silva Nunes Thais Aparecida Vitoriano Dantas Mileny dos Santos de Souza Izabela Nunes do Nascimento Jacinto de Luna Batista Jos Bruno Malaquias 《Entomologia Experimentalis et Applicata》2019,167(6):544-552
The diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is the main pest of brassica crops worldwide. The ringlegged earwig, Euborellia annulipes (Lucas) (Dermaptera: Anisolabididae), has been reported as a potential predator of lepidopteran larvae, including this pest, and may therefore be used for biological control. Knowledge about predator–prey interactions is important to establish pest management strategies. Therefore, the objective of this work was to evaluate the influence of the developmental stage (larva and pupa) and density of P. xylostella on the preference and functional response of E. annulipes adult females. We used choice and no‐choice tests to evaluate the foraging behavior and preference of E. annulipes on DBM life stages and varied prey density to assess the type of functional response of the ringlegged earwig. Larvae were preferred over pupae, and the predator’s functional response was type II for both prey stages. Our results report the potential of E. annulipes as a biocontrol agent of P. xylostella. Understanding their interactions may help in decision‐making and optimization of integrated management strategies. 相似文献
20.
光照时间和冷藏对桃小食心虫生长发育和繁殖的影响 总被引:3,自引:1,他引:3
研究了光照时间和冷藏对桃小食心虫各虫态生长发育和繁殖的影响.结果表明,随着光照时数的延长,各虫态历期基本呈缩短趋势,光照时间对幼虫滞育率影响最为明显,15 h滞育率最低,无论是光照延长还是缩短,幼虫滞育率都会明显增加.其它重要生命参数如单雌产卵量、幼虫体重、孵化率、脱出率和羽化率等最高点多集中在光照13~17 h,所以,桃小食心虫生长发育和繁殖的最佳光照时间为15 h左右.冷藏时间对蛹历期、成虫寿命和卵历期影响均不明显,但冷藏7 d后羽化率、单雌产卵量及孵化率均呈下降趋势,蛹和卵不易长时间低温存放. 相似文献