共查询到18条相似文献,搜索用时 38 毫秒
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对一株野生型的出芽短梗霉(Aureobasidiumpullulans)Ftl和从Ftl出发经原生质体再生筛选出的菌株R45进行了摇瓶发酵产普罗蓝糖的比较研究,结果表明R45无论从形态,菌体生长情况,还是从普罗蓝糖的产量,黑色素的产生等方面都与亲株Ftl有明显的区别,说明R45是一株具有一定生产价值的变异菌株。 相似文献
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在摇瓶发酵条件研究的基础上。于16L自控发酵罐上进行了罐上发酵条件优化研究。发现以10%淀粉水解物为碳源时,淀粉水解物的最适DE值为40-50,发酵培养基中的硫酸铵最适用量不同于摇瓶发酵时的量,种龄和接种量、通气量、罐压、搅拌速度和搅拌叶轮挡数等均对多糖的产生有较大的影响。另外还进行了发酵过程的动力学的研究。 相似文献
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【背景】出芽短梗霉可发酵葡萄糖生成聚苹果酸,但存在转化率和转化效率低等瓶颈,阻碍其实现商业化生产。【目的】通过优化发酵培养条件,提高出芽短梗霉的聚苹果酸产量、糖酸转化率和生产强度。【方法】采用单因素试验优化适宜出芽短梗霉BK-10菌株产生聚苹果酸的培养条件,通过Plackett-Burman法对培养基组分筛选显著性影响因素,并对其培养基中无机盐进行正交试验优化,最后进行5 L发酵罐验证。【结果】最优培养基配方和培养条件:100 g/L葡萄糖,1.5 g/L尿素,0.20 g/L KH_2PO_4,0.20 g/L ZnSO_4,0.05 g/L MgSO_4,0.75 g/L KCl,30 g/L CaCO_3,0.01%吐温-80,发酵温度26°C,250 mL摇瓶装液量50 mL。【结论】通过优化,聚苹果酸的糖酸转化率达到0.71 g/g,生产强度达到0.89 g/(L·h),较优化前分别提高了18.33%和71.15%,为发酵葡萄糖合成聚苹果酸进而生产L-苹果酸工艺的工业化生产奠定经济性基础。 相似文献
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用聚丙烯酰胺凝胶电泳技术对出芽短梗霉的亲本菌株及其4株原生质体再生菌株的脂酶、谷氨酸脱氢酶、细胞色素氧化酶和可溶性蛋白进行了比较。发现各变异菌株之间,变异菌株与亲本菌株之间的谱带均有差异,原生质体再生菌株的变异涉及酶蛋白的改变。 相似文献
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Summary A variety of habitats were sampled for the presence of Aureobasidium black yeasts with the attempt to find pullulan-producing strains. Habitats included leaves of mango (Mangifera indica Linn.), tamarind (Tamarindus indica Linn.), asoka (Saraca indica Linn.) and latex-painted and bathroom cement-wall surfaces. Parameters for the identification of the isolates included morphology, nutritional parameters, exopolysaccharide (EPS) production, and rDNA internal transcribed spacer (ITS) sequencing. All isolates of black yeasts were polymorphic with blastospores, hyphae, and chlamydospores. ITS analyses showed strong correlation with the GenBankA. pullulanssequences, with alignment using BLAST yielding greater than 95% similarity. All five isolates tested produced pullulan as deduced from infrared spectra and sensitivity to pullulanase. None produced aubasidan as evidenced from their IR spectra. The current studies support the notion that the hot, humid environments facilitate the development of A. pullulansand its tropical variants in diverse phylloplane and walls habitats, and merit support for further isolation and characterization of these black yeasts as a source of unique pullulan-producing strains. 相似文献
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R. Gaur R. Singh S. Tiwari S.K. Yadav N.S. Daramwal 《Journal of applied microbiology》2010,109(3):1035-1043
Aims: To isolate the novel nonmelanin pullulan‐producing fungi from soil and to optimize the physico‐chemical and nutritional parameters for pullulan production. Methods and Results: A selective enrichment method was followed for the isolation, along with development of a suitable medium for pullulan production, using shake flask experiments. Pullulan content was confirmed using pure pullulan and pullulanase hydrolysate. Eurotium chevalieri was able to produce maximum pullulan (38 ± 1·0 g l?1) at 35°C, pH 5·5, 2·5% sucrose, 0·3% ammonium sulfate and 0·2% yeast extract in a shake flash culture medium with an agitation rate of 30 rev min?1 for 65 h. Conclusions: The novel pullulan‐producing fungus was identified as E. chevalieri (MTCC no. 9614), which was able to produce nonmelanin pullulan at from poorer carbon and nitrogen sources than Aureobasidium pullulans and may therefore be useful for the commercial production of pullulan. Significance and Impact of the Study: Eurotium chevalieri could produce pullulan in similar amounts to A. pullulans. Therefore, in future, this fungus could also be used for commercial pullulan production, because it is neither polymorphic nor melanin producing, hence its handling during pullulan fermentation will be easier and more economical. 相似文献
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普鲁兰多糖应用现状研究 总被引:2,自引:0,他引:2
YANG Xueling 《武汉生物工程学院学报》2007,(3)
介绍了普鲁兰多糖的理化性质和生理功能,阐述了其在农产品、食品、环保、包装、医药、石油等领域的应用现状,并对其良好的市场前景进行了展望。 相似文献
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Guoqiang Chen Jiming Wang Yulong Su Youshuang Zhu Ge Zhang Hongwei Zhao 《Preparative biochemistry & biotechnology》2017,47(10):963-969
Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. First, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with thin-layer chromatography, Fourier transform infrared, and nuclear magnetic resonance. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4?g/L Tween 80 for 96?hr of cultivation), continuously fermentation was performed. The highest pullulan production and dry biomass was 109 and 125?g/L after fermentation for 114?hr, respectively. The average productivity was about 1?g/L/hr, which was intensively higher than the previous reported. This study would lay foundations for the industrial production of pullulan. 相似文献
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The production of pullulan from brewery wastes by Aureobasidium pullulans in shake flask culture was investigated. The maximum pullulan concentration (6.0g/l) was obtained after 72h of fermentation. The external addition of nutrients into the spent grain liquor improved significantly the production of pullulan. In this case, the highest values of pullulan concentration (11.0±0.5g/l), pullulan yield (48.2±1.5%), and sugar utilization (99.0±0.5%) were obtained in the medium (pH 6.5–7.5) supplemented with K2HPO4 0.5%, l-glutamic acid 1%, olive oil 2.5%, and Tween 800.5%. 相似文献
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聚苹果酸的发酵培养条件优化 总被引:3,自引:0,他引:3
对出芽短梗霉(Aureobasidium pullulans)BS02发酵制备生物降解材料聚苹果酸的摇瓶发酵条件进行研究,确定了出芽短梗霉发酵制备聚苹果酸的摇瓶培养条件。由实验结果可知:优化的培养基(g/L)为葡萄糖120.0、丁二酸铵3.0、丁二酸2.0、MnSO4.H2O 0.005、MgSO4.7H2O 0.1,另外每升发酵液加玉米浆0.5 mL,CaCO350 g/L,培养条件为pH4.0~4.5、24℃、500 mL摇瓶装发酵液100 mL、摇床转速220 r/min,在最优条件下,聚苹果酸产量可达到30 g/L。 相似文献