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1.
Ozkaya M Sahin M Cakal E Gisi K Bilge F Kilinc M 《Biological trace element research》2009,128(2):144-151
The present study was conducted to evaluate the serum selenium levels in first-degree relatives of diabetic patients (FDR)
according to controls. Insulin resistance, serum lipid levels, inflammation markers, and blood pressure were also studied
in these patients. Serum levels of selenium in FDR were significantly lower than control group (74.65 ± 5.9 vs 88.7 ± 8.7 μg/dl,
p < 0.0001). HsCRP, HOMA-IR, insulin, homocysteine levels were significantly higher in FDR according to the control group (1.32 ± 0.9
vs 0.63 ± 0.4 mg/dL, p < 0.0001; 2.07 ± 0.84 vs 1.51 ± 0.69, p < 0.0001; 9.26 ± 3.8 vs 6.8 ± 2.98 μU/MI, p < 0.0001; 15.7 ± 7.4 vs 11.5 ± 5.1 μmol/L, p < 0.0001, respectively). There was significant correlation between selenium levels and hsCRP (r = − 0.450, p < 0.0001). There was also weak significant correlation also between HOMA-IR and selenium levels (r = −0.227, p = 0.003). There was a correlation between systolic blood pressure and BMI (r = 0.365, p < 0.0001). But there was no correlation between selenium levels and blood pressure or other parameters. HsCRP, HOMA-IR, homocysteine
levels in individuals with selenium levels < 80 μg/L (n = 78) was significantly higher than hsCRP HOMA-IR, homocysteine levels in individuals with selenium levels ≥ 80 (n = 91; 1.23 ± 0.98 vs 0.81 ± 0.76 mg/dL, p < 0.003; 1.99 ± 0.88 vs 1.64 ± 0.74, p < 0.005; 15.0 ± 7.6 vs 12.9 ± 5.7 μmol/L, p < 0.049, respectively). Selenium deficiency may contribute to cardiovascular disease risk in FDR. 相似文献
2.
Letsiou S Nomikos T Panagiotakos D Pergantis SA Fragopoulou E Antonopoulou S Pitsavos C Stefanadis C 《Biological trace element research》2009,128(1):8-17
The trace element selenium is an essential micronutrient for human health and its low levels in serum are implicated in the
pathogenesis of several chronic diseases. Therefore, the determination of total selenium in serum may contribute to the assessment
of the health and nutritional status of certain populations. The objective of the present work was to determine total selenium
in the serum of 506 healthy volunteers that participated in the ATTICA study. Selenium was determined in serum by using the
technique of inductively coupled plasma mass spectrometry. The mean serum selenium concentration was determined to be 91.8 ± 33.7 μg/L
(N = 506); 87.6% of women and 88.5% of men had serum selenium concentration below 125 μg/L, the cutoff considered to be required
for optimal glutathione peroxidase activity. No association was found between serum selenium levels and the gender of the
participants while a significant decline of selenium with age (p < 0.0001) was observed. According to our results, no anthropometric, lifestyle, nutritional, or biochemical indices were
able to affect the association between serum selenium and age. This result may indicate that other factors such as selenium
distribution as well as retention may be affecting the relationship between serum selenium and age. 相似文献
3.
4.
P. V. Luoma H. Korpela E. A. Sotaniemi J. Kumpulainen 《Biological trace element research》1985,8(2):113-121
This study evaluated selenium status in relation to lipid peroxidation, liver microsomal function, and serum lipids in humans. Serum selenium concentration, glutathione peroxidase (GSH-Px) activity, liver microsomal enzyme activity, assessed by plasma antipyrine clearance (AP-CL) rate, and serum lipids were determined in 23 healthy subjects in a double-blind placebo-controlled trial of selenium supplementation. The low selenium concentration (74.0±14.2 μg/L, mean±SD) is attributable to the low selenium content of the diet. Subjects with the lowest selenium levels (n=11) had reduced serum GSH-Px activity, AP-CL rate, high-density lipoprotein cholesterol (HDL-C), and total cholesterol (T-C) as compared with subjects with higher selenium concentrations (n=12). Low AP-CL rates were associated with low HDL-C: T-C ratios. Selenium supplementation, 96 μg/d for 2 wk, increased serum selenium, GSH-Px activity, and the HDL-C: T-C ratio. The results suggest that a low serum selenium level is associated with a decrease in liver microsomal enzyme activity and serum HDL-C and T-C concentrations. Selenium supplementation in subjects with low serum selenium may favorably influence relations between serum lipoproteins connected with the development of atherosclerotic vascular disease. 相似文献
5.
Selenium helps protect against peroxidation during aging as part of the glutathione peroxidase (GPx) antioxidant system. Selenium
status, however, is often low in elderly persons who have low selenium intake, live in institutions, and have certain chronic
diseases. In addition, a relationship has been observed between the female reproductive hormone, estrogen, and selenium status,
with blood selenium and GPx activity coinciding with fluctuations in estrogen during the menstrual cycle. These findings suggest
that the decrease in estrogen following menopause may cause a decrease in selenium status, and thus accelerate the process
of aging and increase the risk of certain diseases. The current study compared selenium status in healthy premenopausal (n = 13, 21 to 43 years) and postmenopausal (n = 10, 57 to 86 years) women. Selenium intakes of both groups were similar and greater than the recommended dietary allowance
(RDA) of 55 μg/day for adult women. Although neither plasma nor RBC selenium concentrations were significantly different between
groups, postmenopausal women had significantly greater plasma (p < 0.02), and RBC (p < 0.05) GPx activities compared to premenopausal women possibly in response to oxidative processes associated with aging.
These results indicate that the selenium status of healthy postmenopausal women did not decline with menopause and that their
antioxidant capability, as measured by GPx activity, was preserved with dietary intake of selenium greater than the RDA.
Presented in part at the Experimental Biology 2000, April 2000, San Diego, CA [Smith AM, Ha EJ, Medeiros LC. Selenium-dependent
glutathione peroxidase activity is increased in healthy post-menopausal women. FASEB J 2000;14:A513.]. 相似文献
6.
Jetmund Ringstad Synnøve F. Knutsen Odd R. Nilssen Yngvar Thomassen 《Biological trace element research》1993,36(1):65-71
Depressed selenium and Vitamin E levels may contribute to hepatic injury through lipid peroxidation. To study the effect of
moderate alcohol drinking (32.4±23.6 g ethanol/d) on serum selenium and serum vitamin E concentrations, we conducted a matched-pair
study of 73 healthy, well-nourished risk drinkers and healthy controls with little or no alcohol consumption. Among risk drinkers,
serum selenium was significantly lowered (1.49 vs 1.67 μmol/L;p<0.001) compared with controls. Difference in α-tocopherol concentrations did not, however, reach statistical significance
(22.8 vs 24.9 μmol/L;p=0.06). Nutritional and life-style factors differed very little between the two groups. We conclude that even moderate alcohol
consumption lowers selenium status. Selenium may thus represent a link joining the hepatotoxic and nutritional backgrounds
of alcoholic liver disease. 相似文献
7.
Mustafa Akil Umit Gurbuz Mursel Bicer Abdullah Sivrikaya Rasim Mogulkoc Abdulkerim Kasim Baltaci 《Biological trace element research》2011,142(3):651-659
The present study aims to evaluate the effect of selenium supplementation on lipid peroxidation and lactate levels in rats
subjected to acute swimming exercise. Thirty-two adult male rats of Sprague–Dawley type were divided into four groups. Group
1, control; group 2, selenium-supplemented; group 3, swimming control; group 4, selenium-supplemented swimming group. The
animals in groups 2 and 4 were supplemented with (i.p.) 6 mg/kg/day sodium selenite for 4 weeks. The blood samples taken from
the animals by decapitation method were analyzed in terms of erythrocyte-reduced glutathione (GSH), serum glutathione peroxidase
(GPx) and superoxide dismutase (SOD), and plasma malondialdehyde (MDA) and lactate using the colorimetric method, and serum
selenium values using an atomic emission device. In the study, the highest MDA and lactate values were found in group 3, while
the highest GSH, GPx and SOD values were obtained in group 4 (p < 0,001). Group 2 had the highest and group 3 had the lowest selenium levels (p < 0,001). Results of the study indicate that the increase in free radical production and lactate levels due to acute swimming
exercise in rats might be offset by selenium supplementation. Selenium supplementation may be important in that it supports
the antioxidant system in physical activity. 相似文献
8.
Ten pregnant female camels divided into two groups received, after a 2-week adaptation period, an oral selenium (Se) supplementation
(0 and 2 mg, respectively) under sodium selenite form for 6 months from the three last months of gestation up to the three
first months of lactation. Feed intake was assessed daily. Blood samples and body weight were taken on a biweekly basis, both
in dams and their camel calves after parturition. Feces and urine samples were collected monthly and milk on a biweekly basis.
The Se concentration in serum increased significantly in the supplemented group and was threefold higher than the concentration
compared to the control group, respectively, 305.9 ± 103.3 and 109.3 ± 33.1 ng/mL. The selenium concentration increased in
similar proportion in milk (86.4 ± 39.1 ng/mL in the control group vs 167.1 ± 97.3 ng/mL in treated group), in urine, and
feces. The gluthathione peroxidase (GSH-Px) activity varied between 18.1 ± 8.7 IU/g hemoglobin (Hb) in control group and 47.5 ± 25.6 IU/g
Hb in treated group but decreased after parturition in both groups. Vitamin E did not change significantly and was, on average,
1.17 ± 0.72 and 1.14 ± 0.89 ng/mL in the control and treated groups, respectively. Significant correlations were reported
between serum Se, milk Se, GSH-Px, and fecal and urinary excretion or concentration. Blood values in camel calves were similar
to those of the dams. The results seemed to confirm the sensitivity of camel to Se supplementation with an important increase
of selenium in serum and milk. 相似文献
9.
Mingguang Tan Liqin Sheng Yine Qian Yongxin Ge Yinsong Wang Hongde Zhang Mingli Jiang Guilin Zhang 《Biological trace element research》2001,83(3):231-237
Gestational diabetes is one of the most common diseases in pregnancy. In the present work, the possible relationship between
serum selenium concentration and gestational diabetes was investigated. Blood samples of 234 pregnant women were collected,
including 98 subjects with impaired glucose tolerance (IGT), 46 subjects with gestational diabetes mellitus (GDM), and 90
normal pregnant women (NPW). An additional 17 samples of normal women of fertile age (NW) were collected for comparison. The
hydride generation atomic fluorescence spectrometry was used for selenium determination. The mean serum selenium levels obtained
for each group were 0.0741±0.0167 mg/L for NPW, 0.0631±0.0132 mg/L for IGT, 0.0635±0.0120 mg/L for GDM, and 0.108±0.0170 mg/L
for NW. Serum selenium levels were significantly lower in pregnant woman with IGT (p<0.001) and GDM (p<0.001) than in NPW. Furthermore, an inverse correlation between the serum selenium concentration and the gestational period
was also observed. Selenium supplementation during gestation for pregnant women, especially with IGT and GDM, should be considered. 相似文献
10.
Chistiakov DA Voronova NV Turakulov RI Savost'anov KV 《Journal of applied genetics》2011,52(2):201-207
The human secretoglobin 3A2 (SCGB3A2) gene encoding secretory uteroglobin-related protein 1 (UGRP1) resides on the chromosome region 5q31-33 that harbors a susceptibility
locus to several autoimmune and inflammatory diseases, including asthma and Graves’ disease (GD). Recently, association between
the marker rs1368408 (−112G > A), located in the promoter region of the SCGB3A2 gene, and susceptibility to GD was found in Chinese and UK Caucasians. The study aim was to evaluate whether this polymorphism
confers GD susceptibility in a large population cohort comprising 1,474 Russian GD patients and 1,619 controls. The marker
rs1368408 was studied using a TaqMan allele discrimination assay. Serum levels of UGRP1 and immunoglobulin E (IgE) were assessed
using enzyme-linked immunosorbent assay (ELISA) analyses. Association between the allele A of SCGB3A2 and a higher risk of GD (odds ratio [OR] = 1.33, P = 2.9 × 10−5) was shown. Both affected and non-affected carriers of the higher risk genotype A/A had significantly decreased levels of
serum UGRP1 compared to the subjects homozygous for G/G (93 ± 37 pg/ml vs. 132 ± 45 pg/ml, P = 0.0011 for GD patients; 77 ± 28 pg/ml vs. 119 ± 33 pg/ml, P = 0.0019 for controls). Serum IgE levels were significantly higher in non-affected subjects homozygous for A/A compared to
control individuals homozygous for G/G (153 ± 46 IU/ml vs. 122 ± 40 IU/ml, P = 0.0095). Our data suggest that the carriage of the SCGB3A2 −112A/A variant increases the risk for GD in subsets of patients with elevated levels of IgE, a hallmark of allergic asthma.
Therefore, the SCGB3A2 −112G > A polymorphism may be considered as a likely marker linking susceptibility to allergy/asthma and GD on chromosome
5q31-33. 相似文献
11.
Mine Gulaboglu Bunyamin Borekci Ilhan Delibas 《Biological trace element research》2010,136(3):249-257
The aim of this study was to investigate the urine iodine concentration in women with severe preeclampsia and in healthy women
in Erzurum, Turkey. Urine specimens were obtained from 40 severe preeclampsia and 18 healthy pregnant women. Urinary iodine
levels were determined by the Foss method based on the Sandell–Kolthoff reaction. The urinary iodine level for women with
severe preeclampsia was 4.25 ± 2.7 μg/dL, lower than 20.89 ± 6.4 μg/dL of urinary iodine for healthy pregnant women (p < 0.001). Blood magnesium concentration was found to be 1.63 ± 0.05 mg/dL for women with severe preeclampsia, which is lower
than that of healthy pregnant women (1.87 ± 0.05 mg/dL; p < 0.001). There was a positive correlation between urinary iodine level and blood magnesium level in pregnant women with
preeclampsia (Pearson correlation coefficient = 0.43; p < 0.01). However, there was no correlation between urinary iodine level and blood magnesium level in healthy pregnant women.
There was no difference in thyroid hormone levels (T4, TSH, FT4) between women with severe preeclampsia and healthy pregnant
women. However, there was a difference in T3 thyroid hormone levels between women with severe preeclampsia (1.86 ± 0.4 μg/dL)
and healthy pregnant women (1.45 ± 0.3 μg/dL; p < 0.001). There was also a difference in FT3 between women with severe preeclampsia (2.77 ± 0.4 pg/mL) and healthy pregnant
women (2.41 ± 0.5 μg/dL; p < 0.01). Urinary iodine excretion is currently the most convenient laboratory marker of iodine deficiency. The method is
useful for the rapid and low-cost assessment of iodine deficiency. Our results suggested that urinary iodine concentration
might be a useful marker for prediagnosing preeclamptic women. In addition, iodine supplementation may also be considered
for preeclamptic therapy. 相似文献
12.
Characteristics of Se-Enriched Mycelia by Stropharia rugoso-annulata and its Antioxidant Activities in vivo 总被引:1,自引:0,他引:1
Zhen Song Le Jia Feng Xu Fanyun Meng Peng Deng Keming Fan Xiaonan Liu 《Biological trace element research》2009,131(1):81-89
Selenium (Se) is an essential trace element for humans and animals. Stropharia rugoso-annulata is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this study were to investigate
the Se-enrichment characteristics of S. rugoso-annulata in submerged culture and evaluate the antioxidant activities of Se-enriched mycelia in vivo in terms of the values of glutathione
peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA). The optimum parameters of Se-enrichment under
the optimal Se concentration (150 μg/mL) in media were as follows: biomass 8.11 ± 0.25 g/L, Se content in mycelia 4,727.68 ± 13 μg/g,
Se-accumulated rate 24.68 ± 1.67%, and percentage of organic Se 96.27 ± 3.26%. The mainly subsistent forms of selenium in
Se-enriched mycelia were selenoprotein and selenium-polysaccharide. The contents of total amino acids (TAA) and essential
amino acids (EAA) in Se-enriched mycelia were increased by 13.5 ± 1.09% and 12.8 ± 0.89%, respectively. It was efficient for
Se-enriched mycelia to elevate GSH-Px and SOD activities and decrease MDA content. These results indicated that Se-enriched
mycelia of S. rugoso-annulata represent a novel dietary source of bioavailable supplemental selenium. 相似文献
13.
14.
Selenium concentrations in the blood of 112 (56 females and 56 males) normal subjects, from different regions of the Punjab
(Pakistan), have been determined using the technique of inductively coupled plasma-mass spectrometry. The whole blood selenium
concentrations were found to be 452 ± 12 ppb (parts per billion or nano-gram of Se per gram freeze-dried blood or 96 ± 3 μg/L
), with 470 ± 16 ppb (or 100 ± 4 μg/L) in female and 435 ± 16 ppb (or 92 ± 4 μg/L) in male population. Compared with other
populations of the world, these levels are amongst the lowest. 相似文献
15.
Seema Jain Priyamvada Sharma Shobha Kulshreshtha Govind Mohan Saroj Singh 《Biological trace element research》2010,133(2):162-170
Pre-eclampsia is the most common medical complication of pregnancy associated with increased maternal and infant mortality
and morbidity. Its exact etiology is not known, although several evidences indicate that various elements might play an important
role in pre-eclampsia. This study was carried out to analyze and to compare the concentration of calcium, magnesium, and zinc
in the serum of women with pre-eclampsia and in normal pregnant women. Fifty clinically diagnosed patients with pre-eclampsia
(25 with mild and 25 with severe pre-eclampsia) and 50 normal pregnant controls were enrolled in this study. The serum calcium,
magnesium, and zinc levels were estimated with an atomic absorption spectrophotometer. The mean serum levels of calcium, magnesium,
and zinc in normal pregnant group were 2.45 ± 0.18 mmol/L, 0.79 ± 0.13 mmol/L, and 15.64 ± 2.4 μmol/L, respectively, while
in mild pre-eclamptic group, these were 2.12 ± 0.15 mmol/L, 0.67 ± 0.14 mmol/L, and 12.72 ± 1.7 μmol/L, respectively. Serum
levels in severe pre-eclamptic group were 1.94 ± 0.09 mmol/L, 0.62 ± 0.11 mmol/L, and 12.04 ± 1.4 μmol/L, respectively. These
results indicate that reduction in serum levels of calcium, magnesium, and zinc during pregnancy might be possible contributors
in etiology of pre-eclampsia, and supplementation of these elements to diet may be of value to prevent pre-eclampsia. 相似文献
16.
Pregnant women in developing countries are vulnerable to multiple micronutrient deficiencies. Studies assessing serum levels
of the micronutrients and magnitude of their deficiencies are very scarce in African subjects. This study was aimed at determining
serum levels of micronutrients in 375 pregnant (42 HIV seropositive) and 76 non-pregnant women (20 HIV seropositive) who visited
the University of Gondar Hospital, Gondar, Ethiopia. Serum concentrations of zinc,\ copper, selenium, calcium, and magnesium
were determined using an inductively coupled plasma mass spectrometer. Irrespective of HIV serostatus, pregnant women had
significantly higher serum concentrations of copper and copper/zinc ratio and significantly lower magnesium compared to those
in non-pregnant women (P < 0.05). Except for selenium, which was significantly lower in HIV-seropositive pregnant women (P < 0.05), the mean serum concentrations of zinc, copper, calcium, and magnesium were not significantly different between pregnant
women by HIV serostatus. The prevalence of deficiency in zinc, magnesium, selenium, and calcium in the pregnant women, irrespective
of their HIV serostatus, was 66.7%, 25.6%, 21.9%, and 9.3%, respectively. The magnitude of deficiency in zinc, magnesium,
and selenium was significantly higher in HIV seropositive pregnant women (76.2%, 52.4%, and 45.2%) than that in HIV-seronegative
pregnant women (65.5%, 22.2%, and 18.9%) and in HIV-seronegative non-pregnant women (42.9%, 8.1%, and 30.4%; P < 0.05). Deficiency in one, two, three, or four mineral elements was observed in 44.8%, 14.4%, 9.9%, and 5.1% of the pregnant
women, respectively. Only 25.9% of the pregnant women and 44.7% of the non-pregnant women were not deficient in any of the
micronutrients. The high prevalence of micronutrient deficiencies in pregnant and non-pregnant women in Gondar, Ethiopia warrants
the need for strategies on prevention and control of the deficiencies. 相似文献
17.
Hawkes WC Richter BD Alkan Z Souza EC Derricote M Mackey BE Bonnel EL 《Biological trace element research》2008,122(2):107-121
The essential nutrient selenium is required in microgram amounts [recommended dietary allowance (RDA) = 55 μg/day, 699 nmol/day]
and has a narrow margin of safety (upper tolerable intake limit = 400 μg/day, 5 μmol/day). We conducted a randomized placebo-controlled
study of high-selenium yeast, the form used in most supplements (300 μg/day, 3.8 μmol/day), administered to 42 free-living
healthy men for 48 weeks. Dietary intakes of selenium, macronutrients, and micronutrients were not different between groups
and did not change during the study. Supplementation more than doubled urinary selenium excretion from 69 to 160 μg/day (876
to 2,032 nmol/day). Urinary excretion was correlated with recent selenium intake estimated from 3-day diet records: urinary
selenium excretion = 42 μg/day (533 nmol/day) + 0.132 × dietary selenium intake, p < 0.001. Dietary selenium intake was not significantly correlated with the other indicators of selenium status, presumably
because urinary selenium excretion reflected recent intake, and tissue selenium was homeostatically controlled. After 48 weeks
of supplementation, plasma selenium was increased 60% from 142 to 228 μg/l (1.8 to 2.9 μmol/l), and erythrocyte selenium was
approximately doubled from 261 to 524 μg/l (3.3 to 6.6 μmol/l). Selenium concentrations increased more modestly in hair (56%)
and platelets (42%). Platelets were the only blood component in which glutathione peroxidase activity was significantly related
to selenium content. Selenium levels decreased rapidly after the end of supplementation, and there were no significant differences
in selenium status indicators between groups by week 96. The absorption, distribution, and excretion of selenium from high-Se
yeast were similar to selenium in foods. 相似文献
18.
Several studies have observed an inverse association between serum magnesium and fasting glucose levels. However, there have
been sparse data on Koreans. The present study was designed to evaluate the association between serum magnesium and fasting
serum glucose levels in Korean adults. A total of 949 Korean adults who visited an outpatient at a university hospital were
included in this study. We compared mean values of clinical data according to the tertile of serum magnesium level using analysis
of covariance. Association between serum magnesium and fasting glucose levels was evaluated using multiple regression analysis.
Odds ratio (OR) was calculated to determine the association between hypomagnesemia and abnormal glucose metabolism. Fasting
serum glucose levels decreased significantly from the lowest to the highest tertile of serum magnesium level (108.0 ± 1.4,
106.5 ± 2.1, 102.5 ± 1.7 mg/dl, respectively, p = 0.015), whereas blood pressure and lipid profile showed no significant associations. Serum magnesium level was negatively
associated with fasting serum glucose level (β = −0.114, p = 0.001). Participants with hypomagnesemia had significantly higher OR for abnormal glucose metabolism compared to those
with normomagnesaemia (OR = 2.28, 95%CI 1.29–4.02). There was a negative association between serum magnesium and fasting glucose
levels in Korean adults. In addition, hypomagnesemia was associated with abnormal glucose metabolism. 相似文献
19.
Souvik Roy Sudheer Kumar Dontamalla Anil Kumar Mondru Santanu Sannigrahi Prabhakar Reddy Veerareddy 《Biological trace element research》2011,139(1):55-71
To investigate whether sodium selenate treatment would impact on the onset of diabetic nephropathy, we examined blood glucose,
serum biochemical components, and interrelationship between oxidative stress, TGF-β1, and apoptosis in streptozotocin (STZ)
induced diabetic rats. Sixty male Wistar rats were divided into six groups. Group I (n = 10), normal control; Group II (n = 10), diabetic control; Group III (n = 10), sodium selenate (16 μmoles/kg) + diabetic; Group IV (n = 10), sodium selenate (32 μmoles/kg) + diabetic; Group V (n = 10), sodium selenate (16 μmoles/kg) control; and Group VI (n = 10), sodium selenate (32 μmoles/kg) control. Sodium selenate was administered via orogastric route for 10 weeks. In the
diabetic group, diabetes was induced by single intraperitoneal injection of STZ (50 mg/kg). The levels of blood glucose were
estimated and total cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, creatinine, urea, and albumin
were detected in serum. Antioxidant status was examined by measuring the superoxide dismutase (SOD), catalase, glutathione,
and lipid peroxidation in kidney tissues. Histopathological studies were performed in the kidney tissue sections. The expression
of TGF-β1 was estimated by the immunohistochemical analysis in kidneys. Apoptotic study in kidney was performed using the
TdT-mediated dUTP nick end labeling technique. It was observed that blood glucose, serum, total cholesterol, HDL cholesterol,
triglycerides, creatinine, urea, and albumin were significantly higher in diabetic control groups. Diabetic + sodium selenate
(16 and 32 μmoles/kg) significantly reduced blood glucose, serum, total cholesterol, HDL cholesterol, triglycerides, creatinine,
urea, and albumin levels. Selenium-treated groups significantly increased antioxidant enzyme activities (SOD, catalase, and
glutathione) in kidneys of diabetic rats. All enzyme activities of selenium control groups did not differ compared with the
normal control. Sodium selenate reduces significantly lipid peroxidation in diabetic rats. Cellular architecture of the diabetic
rats was altered whereas sodium selenate administration rectifies the degenerative changes of the kidney. Profound immunopositivity
of TGF-β1 was observed in the glomerular and tubulointerstitial cells of diabetic rat kidney. Immunopositivity of TGF-β1 was
significantly reduced in both low and high dose of sodium-selenate-treated rats (P < 0.05, P < 0.01). High numbers of apoptotic cells were observed in diabetic rats whereas sodium selenate in both doses significantly
reduces the incidence of apoptosis (P < 0.05, P < 0.01). We conclude herein that sodium selenate has the potential to play a significant role in limiting the renal impairment
by altering the apoptosis and TGF-β1 in experimental diabetic rats. 相似文献
20.
Ashok Kumar Krishna Agarwal Salam G. Devi Ram K. Gupta Swaraj Batra 《Biological trace element research》2010,136(1):26-32
The objective of this study is to estimate the prevalence of hypocalcemia and its correlation with dietary intake of calcium
(DICa) and urinary calcium excretion (UCaE) in pregnant women. Healthy pregnant women (n = 543) were enrolled consecutively. DICa was calculated form dietary history. Serum calcium (SCa) and 24-h UCaE was measured.
Student t test and Chi-square tests were used to compare the continuous and categorical data in women with and without hypocalcemia
(SCa ≤ 8.7 mg/dL). Linear regression was applied for determining the independent variables for hypocalcemia. The age and gestation
(mean ± SD) were 21.9 ± 2.5 years and 18.0 ± 3.5 weeks, respectively. The body mass index (BMI; mean ± SD) was 23.3 ± 3.9 kg/m2. Seventy-one percent women had an education of less than 10 years. The DICa and SCa were 325 ± 198 mg and 8.1 ± 1.5 mg/dL,
respectively. The prevalence of hypocalcemia was 66.4% (362/545); all being asymptomatic. There was no significant difference
in women with and without hypocalcemia in terms of weight, BMI, monthly family income, DICa, UCaE, and their obstetric outcome.
Daily dietary calcium intake was less than the recommended dietary allowances. There was a high prevalence of asymptomatic
hypocalcemia in pregnant women of low socio-economic status which was unrelated to their overall nutritional status and daily
calcium intake. It did not have any adverse effect on the immediate pregnancy outcome. 相似文献