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应用本实验建立的三组套式PCR(PCR1、2、3)和一组以前报道的套式PCR(PCR4),对59份外周血淋巴细胞(PBL)DNA样品进行了恶性卡他热病毒(Malignantcatarrhalfevervirus,MCFV)核酸序列的检测。这些样品来自51只羊,以及与羊接触而发病的6头牛和2只鹿。除PCR4外,其它三组PCR都能扩增现有4个角马型MCFV分离株。有6只羊在4组PCR中都呈阴性,其余53份样品经PCR4检测均呈阳性。PCR1只能从45只羊体检出MCFVDNA,未能从牛和鹿体检出病毒DNA。PCR2检测的所有样品均呈阴性。在PCR3扩增中,除2头牛外,其它51份样品均呈阳性。通过Southern杂交和限制性酶切分析,对PCR1-4产物的特异性进行了鉴定。此外,敏感性实验表明,四组PCR的差异也不明显。因此,本实验结果说明MCFV基因组在不同种动物之间发生了变异,羊体内的变异株可能是导致其它反刍动物发病的病原 相似文献
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应用本实验建立的三组套式PCR(PCR1、2、3)和一组以前报道的套式PCR(PCR4),对59份外周血淋巴细胞(PBL)DNA样品进行了恶性卡他病毒核酸序列的检测。这些样品来自51只羊,以及与羊接触而发病的6头牛和2只鹿。除PCR4外,其它三组PCR都能扩增现有4个角马型MCFV分离株。有6只羊在4组PCR中都呈阴性,其余53份样品经PCR4检测均呈阳性。PCR1只有从45只羊体检出MCFVDN 相似文献
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本文采用套式PCR(Nestedpolymerasechainreaction)技术对61例婴儿肝炎综合征(Neonatalhepatitis)患者进行了HCMV(Humancytomegalovirus)检测,结果表明,当只用一对引物进行PCR时,检出33例患者为HCMV阳性,阳性检出率为54.1%;而采用套式PCR后,有17例为HCMV阳性,阳性检出率提高到77.0%。可见套式PCR方法的阳性检出率明显高于单一PCR,并远高于目前使用的其它方法。同时也说明HCMV是导致肝炎综合征的主要病原体之一。此外,灵敏性和特异性试验表明,套式PCR技术的敏感度高、特异性强、简单快速,在临床检测和诊断中具有很大的潜力。 相似文献
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李凤梅 《中国生物工程杂志》1996,16(3):10-12
本文用聚合酶链反应PCR技术对369例男性泌尿生殖道炎患者进行淋球菌(NG),解脲支原体(MPU)和沙眼衣原体(CT)的检测,阳性率分别为35.5%,30.5%和11.6%,同时,我们对高度怀疑为淋病患者74例及63例非淋病性尿道炎患者进行细菌培养和PCR的同时检测,淋病患者细菌培养阳性检测率为31.1%,与92-94年间所进行的培养检测结果基本一致,均低于PCR检测结果(77.1%),解脲支原体培养阳性率为17.5%,亦明显低于PCR结果(30.5%),此外,对同一患者进行NG+MPU和MPU+CTPCR检测,发现约40%的淋病患者并发MPU感染,而MPU和CT复合感染亦在10%左右。上述结果说明PCR方法在性病病原体的诊断中比细菌培养法更为可靠,特别是在多种病原体复合感染情况下更为有效。 相似文献
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神经节苷脂GM_3对小鼠腹腔常驻巨噬细胞(R-M)和Ge-132体内激活的巨噬细胞(Ge-132-M)的磷脂代谢转换有显著的影响,当这两种M在体外用GM_3处理时,表现出[ ̄(32)P]Pi和[ ̄3H]肌醇参入PI降低,参入PIP、PIP_2增加;但在[ ̄(32)P]Pi和[ ̄3H]胆碱参入PC上,R-M与Ge-132-M不同,即GM_3促进同位素前体参入R-M的PC,抑制它们参入Ge-132-M的PC.以上结果表明GM3可能提高了PI或PIP的磷酸激酶的活性,致使[ ̄(32)P]PIP和[ ̄(32)P]PIP_2增多,[ ̄(32)P]PI减少.激活的M(Ge-132-M)本身PC代谢转换率较R-M高,当Ge-132-M再受GM_3刺激,PC代谢转换率降低,这提示GM_3对激活的M的PC代谢转换有调节作用. 相似文献
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逝年来人巨细胞病毒(HCMV)感染率高,检测方法进展人,有巨细胞包涵体细胞(CCIC)检测、病毒分离、免疫检测、原位杂交(ISH)、多聚酶链反应(PCR)等法。目前有人把ISH与PCR结合创立了原位多聚酶链反应法(ISPCR),此法更准确、更敏感特异,更有地HCMV感染的早期快速诊断。本文就ISPCR的原理、程序、特征主临床应用作一综述。 相似文献
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多聚酶链反应(PCR)是80年代后期发展起来的先进的体外基因复制技术,用PCR技术检测各种病原体具有高度灵敏、特异,且简便、快速的特点。本文就PCR在耐新青霉素Ⅰ金黄色葡萄球菌(MRSA)感染诊断中的应用作一综述。 相似文献
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构建遗传图的新方法:单精子PCR分型技术 总被引:1,自引:0,他引:1
构建遗传图的新方法———单精子PCR分型技术①赵书红李奎彭中镇(华中农业大学动物分子生物学与育种研究室,武汉430070)盛志廉(东北农业大学动物科学研究所,哈尔滨150030)ReviewofSingleSpermTypingbyPCRMethod... 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献