共查询到20条相似文献,搜索用时 15 毫秒
1.
A. Yu. Borisov 《Biophysics》2009,54(3):302-307
Some data on the structure and composition of chlorosomes are in conflict with their energy and kinetic characteristics. Among
the latter is the very short excitation lifetime of the dominant pigment C740 in the 3D giant chlorosome (about 1000 pigment
molecules per reaction center). Therewith the excitation transfer from C740 to baseplate bacteriochlorophyll B795 and further
to the main membrane B860 can hardly be efficient. This result was obtained by modeling the energy migration between these
pigment fractions in maximally optimized conditions. The possible reasons and mechanisms responsible for such strong nonphotochemical
quenching of electronic excitations in the pigments of giant chlorosomes are substantiated and discussed. 相似文献
2.
A. Y. Borisov A. P. Gaydukova 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2009,3(4):488-495
Excitation migration in the light-harvesting bacteriochlorophyll complexes LH1 and LH2 of purple bacteria has been studied
in many experimental and theoretical works. According to the widely accepted notions, it proceeds along the descending energy
stairway, B800* → 850* → 875* → P870*, where symbol * stands for excitations in the corresponding BChl fraction. In this paper
we demonstrate the existence of one more way of direct excitation delivery from B800 to B875, bypassing the main route via
B850. The comparative modeling enables the estimation of the mean portion of excitation that uses this minor migration way.
In some real cases it may reach 9–9.5%. The values of the critical distances for excitation migration from B800 to B850 and
from B800 to B875, as well as their values for arbitrary spectral shifts in BChl molecules, are determined. 相似文献
3.
The dynamics of migration of electronic excitations and the efficiency of their trapping in two-dimensional ensembles of molecules were analyzed. Molecules were characterized using the following parameters: the width of long-wavelength bands, the values of extinction and rate constant of deactivation of electronic excitations, critical distances of migration close to those of dye molecules, in particular, bacteriochlorophyll a and purple bacteria. A comparative analysis of two-dimensional models of energy migration made it possible to chose a model with an optimum light-harvesting on traps from the largest numbers of light-absorbing molecules. It was shown that in ensembles of molecules having different spectral characteristics (spectral shifts between the short- and long-wavelength fractions of the molecules are hear 800 cm-1) the efficiency of excitation trapping is approximately 90 and 80% for the number of light-harvesting molecules per one trap 210 and 580, respectively. 相似文献
4.
Borisov AIu 《Biofizika》2011,56(6):1147-1152
The main motive which stimulated author in writing this paper was a series of remarks by the reviewers of his articles. Some reviewers stated that Ferster' theory can not represent excitation migration in cases when electronic excitations are delocalized in several molecules. Two of reviewers even directly have proclaimed about "out of date Ferster' theory". This question is evidently of general importance. Therefore this paper contains the detailed analysis of the types of molecular ensembles and conditions which enable one to use correctly the Ferster' theory of inductive resonance. 相似文献
5.
Many approaches to discovering the interaction energy of molecular transition dipoles use the well-known coefficient xi(phi, psi (1) psi (2)) = (cos phi - 3 cos psi (1) cos psi (2))(2), where phi, Psi (1), and Psi (2) are inter-dipole angles. Unfortunately, this formula often yields rather approximate results, in particular, when it is applied to closely positioned molecules. This problem is of great importance when dealing with energy migration in photosynthetic organisms, because the major part of excitation transfers in their chlorophyllous antenna proceed between closely positioned molecules. In this paper, the authors introduce corrected values of the orientation factor for several types of mutual orientation of molecules exchanging with electronic excitations for realistic ratios of dipole lengths and spacing. The corrected magnitudes of interaction energies of neighboring bacteriochlorophyll molecules in LH2 and LH1 light-absorbing complexes are calculated for the class of photosynthetic purple bacteria. Some advantageous factors are revealed in their mutual positions and orientations in vivo. 相似文献
6.
Vibrational excitations of low frequency collective modes are essential for functionally important conformational transitions in proteins. Here we report the first direct measurement on the lifetime of vibrational excitations of the collective modes at 87 pm (115 cm-1) in bacteriorhodopsin, a transmembrane protein. The data show that these modes have extremely long lifetime of vibrational excitations, over 500 picoseconds, accommodating 1500vibrations. We suggest that there is a connection between this relativelyslow anharmonic relaxation rate of approximately 10 g sec-1 and thesimilar observed rate of conformational transitions in proteins, which require require multi-level vibrational excitations and energy exchanges with othervibrational modes and collisional motions of solvent molecules. 相似文献
7.
Recent findings on the photophysical investigations of several cofacial bisporphyrin dyads for through space singlet and triplet energy transfers raised several serious questions about the mechanism of the energy transfers and energy migration in the light harvesting devices, notably LH II, in the heavily studied purple photosynthetic bacteria. The key issue is that for simple cofacial or slipped dyads with controlled geometry using rigid spacers or spacers with limited flexibilities, the fastest possible rates for singlet energy transfer for three examples are in the 10 x 10(9)s(-1) (i.e. just in the 100 ps time scale) for donor-acceptor distances approaching 3.5-3.6 A. The reported time scale for energy transfers between different bacteriochlorophylls, notably B800*-->B850, is in the picosecond time scale despite the long Mg...Mg separation of approximately 18 A. Such a short rate drastically contrasts with the well accepted F?rster theory. This article reviews the modern knowledge of the structure, bacteriochlorophyll a transition moments, and photophysical processes and dynamics in LH II, and compares these parameters with the recently investigated model bisporphyrin dyads build upon octa-etio-porphyrin chromophores and rigid and semi-rigid spacers. The recently discovered role of the rhodopin glucoside residue called carotenoid will be commented as the possible relay for energy transfer, including the possibility of uphill processes at room temperature. In this context, the concept of energy migration, called exciton, may also be affected by relays and uphill processes. Also, it is becoming more and more apparent that the presence of an irreversible electron transfer reaction at the reaction center, i.e. electron transfer from the special pair to the phyophytin macrocycle and so on, renders the rates for energy transfer and migration more rapid precluding all possibility of back transfers. 相似文献
8.
A. Yu. Borisov 《Biochemistry. Biokhimii?a》2014,79(3):227-234
Based on currently available data, the energy transfer efficiency in the successive photophysical and photochemical stages has been analyzed for purple bacteria. This analysis covers the stages starting from migration of the light-induced electronic excitations from the bulk antenna pigments to the reaction centers up to irreversible stage of the electron transport along the transmembrane chain of cofactors-carriers. Some natural factors are revealed that significantly increase the rates of efficient processes in these stages. The influence on their efficiency by the “bottleneck” in the energy migration chain is established. The overall quantum yield of photosynthesis in these stages is determined. 相似文献
9.
The writing of this paper has been driven by a series of remarks made by reviewers of author’s works in several journals. They asserted that the Förster theory of inductive resonance is inapplicable in cases when electronic excitations are delocalized over several molecules, or plainly dismissed the theory as “out of date”. Since this is doubtlessly a question of general importance, this paper offers a detailed analysis of the types of molecular ensembles and conditions where the use of Förster theory is well founded.
相似文献10.
Érica Cristina Moreno Nascimento João B. L. Martins 《Journal of molecular modeling》2011,17(6):1371-1379
Hartree-Fock and density functional methods were used to analyze electronic and structural properties of known drugs to evaluate
the influence of these data on acetylcholinesterase inhibition. The energies of the frontier orbitals and the distances between
the more acidic hydrogen species were investigated to determine their contributions to the activity of a group of acetylcholinesterase
inhibitors. Electrostatic potential maps indicated suitable sites for drugs-enzyme interactions. In this study, the structural,
electronic and spatial properties of nine drugs with known inhibitory effects on acetylcholinesterase were examined. The data
were obtained based on calculations at the B3LYP/6-31 + G(d,p) level. Multivariate principal components analysis was applied
to 18 parameters to determine the pharmacophoric profile of acetylcholinesterase inhibitors. Desirable features for acetylcholinesterase
inhibitor molecules include aromatic systems or groups that simulate the surface electrostatic potential of aromatic systems
and the presence of a sufficient number of hydrogen acceptors and few hydrogen donors. PCA showed that electronic properties,
including the HOMO-1 orbital energy, logP and aromatic system quantity, as well as structural data, such as volume, size and
H-H distance, are the most significant properties. 相似文献
11.
Increasing stability reduces conformational heterogeneity in a protein folding intermediate ensemble
Sridevi K Lakshmikanth GS Krishnamoorthy G Udgaonkar JB 《Journal of molecular biology》2004,337(3):699-711
A multi-site, time-resolved fluorescence resonance energy transfer methodology has been used to study structural heterogeneity in a late folding intermediate ensemble, IL, of the small protein barstar. Four different intra-molecular distances have been measured within the structural components of IL. The IL ensemble is shown to consist of different sub-populations of molecules, in each of which one or more of the four distances are native-like and the remaining distances are unfolded-like. In very stable conditions that favor formation of IL, all four distances are native-like in most molecules. In less stable conditions, one or more distances are unfolded-like. As stability is decreased, the proportion of molecules with unfolded-like distances increases. Thus, the results show that protein folding intermediates are ensembles of different structural forms, and they demonstrate that conformational entropy increases as structures become less stable. These observations provide direct experimental evidence in support of a basic tenet of energy landscape theory for protein folding, that available conformational space, as represented by structural heterogeneity in IL, becomes restricted as the stability is increased. The results also vindicate an important prediction of energy landscape theory, that different folding pathways may become dominant under different folding conditions. In more stable folding conditions, uniformly native-like compactness is achieved during folding to IL, whereas in less stable conditions, uniformly native-like compactness is achieved only later during the folding of IL to N. 相似文献
12.
Borisov AIu 《Biofizika》2012,57(2):243-246
It is shown that the results provided in a variety of publications, which deal with structural characterization of green bacteria chlorosoma, are in explicit contradiction with kinetic and energy characteristics of microorganisms studied. The data on chlorosoma structure and composition represent no explanation as to how the additional quantity of electronic excitations generated by light in its dominating pigment C750 feeds the main photosystem.. In order to reveal the contradictions, the structural and spectral data on chlorosoma are analyzed in cooperation with the theory of inductive resonance developed by T. Ferster. 相似文献
13.
14.
Theory and application of fluorescence homotransfer to melittin oligomerization. 总被引:5,自引:2,他引:3
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Fluorescence homotransfer (electronic energy transfer between identical fluorophores) has the potential to quantitate the number of subunits in membrane protein oligomers. Homotransfer strongly depolarizes fluorescence emission as a result of intermolecular excitation energy exchange between an initially excited, oriented molecule and a randomly oriented neighbor. We have theoretically treated fluorescein labeled subunits in an oligomer as a cluster of molecules that can exchange excitation energy back and forth among the subunits within that group. We find that the larger the number of subunits, the more depolarized is the emission. The general equations to calculate the expected anisotropy for complexes composed of varying numbers of labeled subunits are presented. Self-quenching of fluorophores, orientation, and changes in lifetime are also discussed and/or considered. To test this theory, we have specifically labeled melittin on its N-terminal with fluorescein and monitored its monomer to tetramer equilibrium both in solution and in lipid bilayers. The calculated anisotropies are close to the experimental values when non-fluorescent fluorescein dimers are taken into account. Our results show that homotransfer may be a promising method to study membrane-protein oligomerization. 相似文献
15.
We have calculated the electronic energy loss of proton and α-particle beams in dry DNA using the dielectric formalism. The electronic response of DNA is described by the MELF-GOS model, in which the outer electron excitations of the target are accounted for by a linear combination of Mermin-type energy-loss functions that accurately matches the available experimental data for DNA obtained from optical measurements, whereas the inner-shell electron excitations are modeled by the generalized oscillator strengths of the constituent atoms. Using this procedure we have calculated the stopping power and the energy-loss straggling of DNA for hydrogen- and helium-ion beams at incident energies ranging from 10 keV/nucleon to 10 MeV/nucleon. The mean excitation energy of dry DNA is found to be I = 81.5 eV. Our present results are compared with available calculations for liquid water showing noticeable differences between these important biological materials. We have also evaluated the electron excitation probability of DNA as a function of the transferred energy by the swift projectile as well as the average energy of the target electronic excitations as a function of the projectile energy. Our results show that projectiles with energy ?100 keV/nucleon (i.e., around the stopping-power maximum) are more suitable for producing low-energy secondary electrons in DNA, which could be very effective for the biological damage of malignant cells. 相似文献
16.
Energetics and morphology of sockeye salmon: effects of upriver migratory distance and elevation 总被引:9,自引:0,他引:9
G. T. Crossin†‡ S. G. Hinch† A. P. Farrell§ D. A. Higgs¶ A. G. Lotto† J. D. Oakes¶ M. C. Healey† 《Journal of fish biology》2004,65(3):788-810
Depending on population, wild Fraser River sockeye salmon Oncorhynchus nerka travel distances of <100 km to >1100 km and ascend elevations ranging from near sea‐level to 1200 m to reach spawning areas. Populations embarking on distant, high elevation migrations ( i.e . Early Stuart, Chilko and Horsefly populations) began their upriver spawning migrations with higher densities of somatic energy ( c . 9·2 to 9·8 MJ kg−1 ) and fewer eggs ( c . 3200 to 3800) than populations making shorter, low elevation migrations ( i.e . Weaver and Adams; c . 7·1 to 8·3 MJ kg−1 gross somatic energy and c . 4300 to 4700 eggs). Populations making difficult upriver migrations also had morphologies that were smaller and more fusiform than populations making less difficult migrations, traits that may facilitate somatic energy conservation by reducing transport costs. Indeed, fish travelling long distances expended less somatic energy per unit of migratory difficulty than those travelling shorter distances (2·8 to 3·8 kJ v . 10–1400 kJ). Consistent with evolutionary theory, difficult migrations appear to select for energy efficiency but ultimately fish making more difficult migrations produce fewer eggs, even when differences in body length have been accounted for. Despite large among‐population differences in somatic energy at the start of upriver migration, all populations completed migration and spawning, and subsequently died, with c . 4 MJ kg−1 of energy remaining, a level which may reflect a threshold to sustain life. 相似文献
17.
18.
A semiempirical theory of saccharide optical activity indicates that the dominant source of NaD rotation is a vacuum-uv CD band near 150 nm, a band observed experimentally in polysaccharide film CD spectra. The model is a modification of polarizability theory in which high-energy electronic excitations are coupled by degenerate perturbation theory, giving rise to “molecular excitons.” The existence of an excitation mode well separated in energy from even higher energy modes arises from the local symmetry of tetrahedral carbon atoms in a puckered ring structure. Calculated NaD rotations correlate well with experimental values. 相似文献
19.
Donor-donor energy migration for determining intramolecular distances in proteins: I. Application of a model to the latent plasminogen activator inhibitor-1 (PAI-1).
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A new fluorescence spectroscopic method is presented for determining intramolecular and intermolecular distances in proteins and protein complexes, respectively. The method circumvents the general problem of achieving specific labeling with two different chromophoric molecules, as needed for the conventional donor-acceptor transfer experiments. For this, mutant forms of proteins that contain one or two unique cysteine residues can be constructed for specific labeling with one or two identical fluorescent probes, so-called donors (d). Fluorescence depolarization experiments on double-labeled Cys mutant monitor both reorientational motions of the d molecules, as well as the rate of intramolecular energy migration. In this report a model that accounts for these contributions to the fluorescence anisotropy is presented and experimentally tested. Mutants of a protease inhibitor, plasminogen activator inhibitor type-1 (PAI-1), containing one or two cysteine residues, were labeled with sulfhydryl specific derivatives of 4,4-difluoro-4-borata-3a-azonia-4a-aza-s-indacence (BODIPY). From the rate of energy migration, the intramolecular distance between the d groups was calculated by using the Forster mechanism and by accounting for the influence of local anisotropic orientation of the d molecules. The calculated intramolecular distances were compared with those obtained from the crystal structure of PAI-1 in its latent form. To test the stability of parameters extracted from experiments, synthetic data were generated and reanalyzed. 相似文献
20.
Vasil'kov SL 《Biofizika》2003,48(1):27-34
Formulae were obtained for the quantitative analysis of the following parameters of excitons in DNA: 1) the lifetime of electronic excitation; 2) the numbers of exciton runs along the DNA sequence; 3) the energy loss by an exciton for one run; 4) the maximum length of the DNA sequence capable of deactivating an exciton for one run. The maximum and minimum ranges for the constant of electronic excitation migration was determined to meet the requirement of inductive-resonance energy transfer for the case of strong interaction. The constant of exciton energy migration was shown to depend on the activation energy, which is equal to the energy of absorbed quantum. An analytical formula was derived to determine the number of quanta the DNA molecule is able to absorb, depending on its length, without nonlinear effects (without overlapping of spatial areas of electronic excitation). By this formula, DNA sequences consisting of only identical AT and GC nucleotide pairs and aggregate AT + GC (in the ratio 1:1) DNA sequences ranging from 1 up to 10(10) base pairs were analyzed. The results of the analysis suggest that the overlapping of spatial areas of electronic excitation induced by a single ultraviolet quantum occurs in short DNA sequences characteristic of prokaryotes. To achieve the same effects on long DNA sequences specific for eukaryotes, DNA must synchronously absorb a great number of ultraviolet quanta. Based on the above results, the following conclusions were made: 1) disturbances in the normal activity of DNA and RNA polymerases may be due to electromagnetic field, which is caused by the oscillatory relaxation of vibronic levels of nucleotides. The energy enters the vibronic levels of nucleotides from an exciton running along the DNA sequence; 2) the increase in the noncoding DNA sequences in eukaryotes due to evolution is a way of DNA protection from undesirable mutations; 3) prokaryotes must possess a greater potentiality and a higher rate of mutagenesis in comparison with eukaryotes, which is proved by their greater diversity in nature. 相似文献