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1.
DL-Glutamic acid has been resolved into optically active forms as the diastereoisomeric salt of optically active neutral amino acid amides, and the salt is easily converted to the sodium salt of the active forms. By resolution with L-tyrosinamide and L-leucinamide, sodium L-glutamate was obtained in 65 and 80 per cent yield respectively. Attempts to extend this method to resolution of DL-glutamic acid using L-phenylalaninamide as resolving agent resulted in poor yield of less pure D-glutamic acid.

By the infrared spectroscopy and X-ray diffraction analysis it has been confirmed that the diastereoisomeric salts of L-leucinamide or L-tyrosinamide with L- or D-glutamic acid compose a combined salt structure in solid state, whereas L-phenylalaninamide with L- or D-glutamic acid does not compose a characteristic diastereoisomeric salt but rather the mechanical mixture of L-phenylalaninamide and L- or D-glutamate anion in solid state.

In the previous study1), it was reported that L-leucinamide forms the characteristic diastereoisomeric salts with racemic N-acyl mono amino acids, most of which are fairly resolved into their antipodes.  相似文献   

2.
The murI gene encoding D-glutamate racemase plays an important role in the biosynthesis of D-glutamic acid, an essential component of cell wall peptidoglycan of almost all eubacteria. A DNA fragment that could rescue the auxotrophy of D-glutamic acid in the Escherichia coli murI mutant strain WM335 was isolated from Brevibacterium lactofermentum ATCC 13869 belonging to the coryneform bacteria. DNA sequencing reveals that it encodes a protein of 284 amino acid residues, which shows a high level of homology with D-glutamate racemases from several other bacteria.  相似文献   

3.
Mutagenesis induced with nitrous acid and subsequent selection allowed a genetically stable mutant strain,Streptomyces sp. Z-11-6, to be obtained, whose L-glutamate oxidase activity was 40-fold higher than that of the original natural isolate and was as great as 1.6–1.8 units/ml of culture liquid. A procedure for the isolation and purification of the enzyme was developed; the biochemical properties of the enzyme were studied. Out of 20 amino acids tested (including D-glutamate), the glutamate oxidase fromStreptomyces sp. Z-11-6 was active only with L-glutamate. This allows the concentration of L-glutamate to be determined in the presence of other amino acids. Calcium chloride at a concentration of 0.1–0.5% promoted the secretion of the extracellular glutamate oxidase.  相似文献   

4.
Sangavai  C.  Chellapandi  P. 《Amino acids》2019,51(9):1397-1407

Acetoanaerobium sticklandii DSM 519 is a hyper-ammonia-producing anaerobe. It has the ability to produce organic solvents and acids from protein catabolism through Stickland reactions and specialized pathways. Nevertheless, its protein catabolism-directed biofuel production has not yet been understood. The present study aimed to decipher such growth-associated metabolic potential of this organism at different growth phases using metabolic profiling. A seed culture of this organism was grown separately in metabolic assay media supplemented with gelatin and or a mixture of amino acids. The extracellular metabolites produced by this organism were qualitatively analyzed by gas chromatography–mass spectrometry platform. The residual amino acids after protein degradation and amino acids assimilation were identified and quantitatively measured by high-performance liquid chromatography (HPLC). Organic solvents and acids produced by this organism were detected and the quantity of them determined with HPLC. Metabolic profiling data confirmed the presence of amino acid catabolic products including tyramine, cadaverine, methylamine, and putrescine in fermented broth. It also found products including short-chain fatty acids and organic solvents of the Stickland reactions. It reported that amino acids were more appropriate for its growth yield compared to gelatin. Results of quantitative analysis of amino acids indicated that many amino acids either from gelatin or amino acid mixture were catabolised at a log-growth phase. Glycine and proline were poorly consumed in all growth phases. This study revealed that apart from Stickland reactions, a specialized system was established in A. sticklandii for protein catabolism-directed biofuel production. Acetone–butanol–ethanol (ABE), acetic acid, and butyric acid were the most important biofuel components produced by this organism. The production of these components was achieved much more on gelatin than amino acids. Thus, A. sticklandii is suggested herein as a potential organism to produce butyric acid along with ABE from protein-based wastes (gelatin) in bio-energy sectors.

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5.
Of 53 strains of lactic acid bacteria and Kocuria, screened for production or degradation of biogenic amines, 29 Kocuria varians and four strains of Enterococcus faecalisproduced tyramine and, at lower concentrations, histamine. In contrast, Lactobacillus strains that did not possess amino acid decarboxylase activity degraded tyramine. The greatest tyramine oxidase activity was present in the strains L. casei CRL705 (98% degradation) and CRL678 (93%) as well as in L. plantarum CRL681 (69%) and CRL682 (60%).  相似文献   

6.
Summary The amino acid sequence of D-amino acid oxidase from Rhodotorula gracilis was determined by automated Edman degradation of peptides generated by enzymatic and chemical cleavage. The enzyme monomer contains 368 amino acid residues and its sequence is homologous to that of other known D-amino acid oxidases. Six highly conserved regions appear to have a specific role in binding of coenzyme FAD, in active site topology and in peroxisomal targeting. Moreover, Rhodotorula gracilis D-amino acid oxidase contains a region with a cluster of basic amino acids, probably exposed to solvent, which is absent in other D-amino acid oxidases.  相似文献   

7.
Mutagenesis induced with nitrous acid and subsequent selection allowed a genetically stable mutant strain, Streptomyces sp. Z-11-6, to be obtained, whose L-glutamate oxidase activity was 40-fold higher than that of the original natural isolate and was as great as 1.6-1.8 units/ml of culture liquid. A procedure for the isolation and purification of the enzyme was developed; the biochemical properties of the enzyme were studied. Out of 20 amino acids tested (including D-glutamate), the glutamate oxidase from Streptomyces sp. Z-11-6 was active only with L-glutamate. This allows the concentration of L-glutamate to be determined in the presence of other amino acids. Calcium chloride at a concentration of 0.1-0.5% promoted the secretion of the extracellular glutamate oxidase.  相似文献   

8.
The distribution of amino acid racemase activities was investigated in the cell-free extracts of various strains of bacteria. Alanine racemase activity was exclusively found in all the strains tested. However, the cell-free extract of Strain 25-3, which has been identified as Pseudomonas striata, possessed the high activity catalyzing the racemization of alanine, α-aminobutyrate, leucine and methionine. The new and sensitive assay method of amino acid racemase with d-amino acid oxidase and 3-methyl-2-benzothiazolone hydrazone hydrochloride was established.

A new amino acid racemase catalyzing the conversion of either d or l enantiomorph of leucine and α-aminobutyrate to the racemates, was partially purified from the cell-free extract of Pseudomonas striata. Both the racemase reactions are suggested to be catalyzed by a single enzyme because of the constant ratio between the activities during the purification, and of their very resemble behavior to pH, temperature and heating the enzyme. Pyridoxal phosphate functions as the coenzyme for this racemase.  相似文献   

9.
To clarify biodegradation pathways of isoalkyl substituted aromatic hydrocarbons, oxidation products of isopropylbenzene and isobutylbenzene by Ps. desmolytica S449B1 and Ps. convexa S107B1 were examined.

Oxidation products from isopropylbenzene were determined to be 3-isopropylcatechol and (+)-2-hydroxy-7-methyI-6-oxooctanoic acid. Isobutylbenzene was also oxidized to 3-isobutylcatechol and (+)-2-hydroxy-8-methyl-6-oxononanoic acid by the same strains.

From these results, the existence of an unknown reductive step in the degradation of these isoalkyl substituted aromatic hydrocarbons and the initial oxidation of these aromatic hydrocarbons by the strains were made clear. The degradation pathways of isopropylbenzene and isobutylbenzene by these strains were discussed.  相似文献   

10.
The present study is aimed at the naphthalene degradation with and without biosurfactant produced from Pseudomonas aeruginosa isolated from oil-contaminated soil. The present study was carried out to isolate the bacterial strains for the naphthalene degradation and also for biosurfactant production. The isolated strains were screened for their ability to degrade the naphthalene by the methods of optimum growth rate test and for the production of biosurfactants by cetyltrimethylammonium bromide, blood agar medium, and thin-layer chromatography. The present study also focused on the effect of biosurfactant for the degradation of naphthalene by isolate-1. Two bacterial strains were isolated and screened, one for biodegradation and another for biosurfactant production. The second organism was identified as Pseudomonas aeruginosa by 16S rRNA analysis. The purified biosurfactant reduces the surface tension of water and also forms stable emulsification with hexadecane and kerosene. The end product of naphthalene degradation was estimated as salicylic acid equivalent by spectrophotometric method. The results demonstrated that Pseudomonas aeruginosa has the potential to produce biosurfactant, which enhances the biodegradation of naphthalene. The study reflects the potential use of biosurfactants for an effective bioremediation in the management of contaminated soils.  相似文献   

11.
An oil-degrading bacterium identified as Acinetobacter lwoffi was isolated by elective culture on North Sea Forties crude oil from an activated sludge sample. It grew on a wide range of n-alkanes (C12–C28) and 1-phenylalkanes, including 1-phenyldodecane, 1-phenyltridecane and 1-phenyltetradecane. The organism degraded 1-phenyldodecane to phenylacetic acid which was further metabolized via homogentisic acid, whilst 1-phenyltridecane was transformed to trans-cinnamic and 3-phenylpropionic acid which were not further metabolized. Evidence dence is presented for a relationship between aromatic amino acid catabolism and 1-phenyldodecane degradation in this organism.  相似文献   

12.
Chorismate is a key intermediate in the biosynthesis of aromatic amino acids and other natural products in microorganisms and plants. Four chorismate mutase-deficient strains ofEscherichia coliwere evaluated for the large scale microbiological production of this metabolite which is needed for detailed biochemical and structural studies of chorismate-utilizing enzymes. Optimization of culturing and isolation conditions with one of these strains yielded an improved protocol for the production of chorismate in yields that compare favorably with those previously obtained withKlebsiella pneumoniaestrain 62-1, a Class 2 pathogenic organism. A simple, single-step procedure for the purification of large quantities of chorismic acid by C18 reverse phase silica gel flash chromatography is also described. Chorismic acid obtained through this procedure is 90–98% pure and can be stored as the free acid in crystalline form over several months without decomposition.  相似文献   

13.
Summary Aspergillus niger NRRL-3, an organism used for the industrial scale production of d-gluconic acid and glucose oxidase (EC 1.1.3.4), was subjected to mutagenesis and selection for acid production on diagnostic media containing methyl red. The plates contained 0.1 M d-glucose, a concentration that does not produce a color change in the medium surrounding mycelia of the parental strain under the conditions employed. Mutagenized spores yielded occasional colonies which were able to grow rapidly and were surrounded by a reddish zone. A number of such presumptive mutants were selected and isolated. Twenty-six such strains were grown in shaken cultures with liquid media containing 0.01, 0.1 or 0.5 M d-glucose, harvested, disrupted and the specific activity of d-glucose oxidase determined. Seven of the mutant strains had glucose oxidase specific activities markedly higher than the parental strain.Paper No. 8393, Nebraska Agricultural Research Division.  相似文献   

14.
l-Amino acid oxidase is synthesized in Neurospora crassa in response to three different physiological stimuli: (i) starvation in phosphate buffer, (ii) mating, and (iii) nitrogen derepression in the presence of amino acids. During starvation in phosphate buffer, or after mating, l-amino acid oxidase synthesis occurred in parallel with that of tyrosinase. Exogenous sulfate repressed the formation of the two enzymes in starved cultures, but not in mated cultures. Sulfate repression was relieved by protein synthesis inhibitors, suggesting that the effect of sulfate required the synthesis of a metabolically unstable protein repressor. With amino acids as the sole nitrogen source only l-amino acid oxidase was produced. Under these conditions enzyme synthesis was repressed by ammonium and was insensitive to sulfate. Biochemical evidence suggested that the l-amino acid oxidase formed under the three different conditions was the same protein. Therefore, the expression of l-amino acid oxidase appeared to be under the control of least two regulatory circuits. One, also controlling tyrosinase, seems to respond to developmental signals related to sexual morphogenesis. The other, controlling other enzymes of the nitrogen catabolic system, is used by the organism to obtain nitrogen from alternative sources such as proteins and amino acids.  相似文献   

15.
The location of the mucopeptide in the cell wall of Bacteroides convexus was determined by electron microscope after enzymatic and chemical treatment (papain, pepsin, lysozyme and phenol). In the five layered cell wall the innermost electron dense layer (or a part of it) proved to be the mucopeptide. The molar ratio of amino sugar and amino acid components of purified mucopeptide was about 1:1:1:1:1:1 for glucosamine, muramic acid, L-alanine, D-glutamic acid, DL(meso)-diaminopimelic acid and D-alanine.  相似文献   

16.
Oxidation of pyridoxine-P and pyridoxamine-P to pyridoxal-P, inhibition and reactivation of the oxidases were investigated, using the Alcaligenes faecalis oxidase and the Azotobacter agilis oxidase catalyzing. Zone electrophoretic experiments indicated that the oxidases obtained from Alcaligenes faecalis and Azotobacter agilis moved to cathode and anode, respectively, under the same conditions. The oxidation-reduction potential of the both oxidase was found to be about ?50 mV. The oxidation of both pyridoxine-P and pyridoxamine-P was strongly inhibited by pyridoxal-P, pyridoxal, pyridine-4-aldehyde and 4-pyridoxic acid phosphate. This inhibition was markedly decreased by Tris-HCl buffer, and other amino compounds that form Schiff’s base of pyridoxal-P.

An enzyme “pyridoxamine-P transaminase” which catalyzed the transamination between pyridoxamine-P and α-ketoglutaric acid was found in certain anaerobic bacteria, such as Clostridium acetobutylicum, Cl. kainantoi, Cl. kaneboi and Cl. butyricum. The pyridoxamine-P transaminase in the cell-free extract of Cl. kainantoi was purified and some properties were investigated. α-Ketoglutaric acid appeared to be the dominant amino acceptor. Pyridoxamine-P was found to be active as amino donor, but other amino compounds were inert. Since the results were inconclusive, the possibility of vitamin B6-enzyme of pyridoxamine-P transaminase was not shown by the inhibitor studies. Physiological role of the pyridoxamine-P transaminase was discussed in the relation to vitamin B6 metabolism in anaerobic bacteria.  相似文献   

17.
Summary In contrast to chemically induced mutants of tobacco mosaic virus (TMV) in which we have found replacement of one or at most of two amino acids per coat protein chain, the protein chains of naturally occurring TMV strains differ from each other in numerous positions. The complete amino acid sequence of the naturally occurring TMV straindahlemense isolated byMelchers (1940) has been determined. It differs in 30 of the 158 amino acid positions from the TMV wild strainvulgare (Fig. 1). This is the first case in which complete amino acid sequences of the coat proteins of two virus strains can be compared. Such a comparison permits conclusions about the structure of the protein subunits and about certain aspects of the genetic code to be drawn.The electrophoretic mobility curves for the virus rods and the A proteins ofvulgare anddahlemense (Fig. 4) can be explained on the basis of the amino acid sequences of the two strains. Spatial distribution of the positive and negative groups within the protein subunits are discussed. One particular segment of the protein chain appears to be so important for the secondary and/or tertiary structure of the protein subunit that amino acid replacements within this segment in general lead to a loss of infectivity.The 46 cases in which we have exactly located the positions of amino acid differences betweenvulgare and various TMV mutants and strains are summarized in Table 1. Combination of the data in Table 1 with the base compositions of the triplets as obtained from the cell free system ofE. coli permits conclusions about the nucleotide sequence within the triplets to be drawn. The triplets shown in Table 2 represent, at present, the best agreement between the data from the cell free system and the work with TMV mutants.

Mit 4 Textabbildungen  相似文献   

18.
Oxalic acid plays major role in the pathogenesis by Sclerotinia sclerotiorum; it lowers the pH of nearby environment and creates the favorable condition for the infection. In this study we examined the degradation of oxalic acid through oxalate oxidase and biocontrol of Sclerotinia sclerotiorum. A survey was conducted to collect the rhizospheric soil samples from Indo-Gangetic Plains of India to isolate the efficient fungal strains able to tolerate oxalic acid. A total of 120 fungal strains were isolated from root adhering soils of different vegetable crops. Out of 120 strains a total of 80 isolates were able to grow at 10?mM of oxalic acid whereas only 15 isolates were grow at 50?mM of oxalic acid concentration. Then we examined the antagonistic activity of the 15 isolates against Sclerotinia sclerotiorum. These strains potentially inhibit the growth of the test pathogen. A total of three potential strains and two standard cultures of fungi were tested for the oxalate oxidase activity. Strains S7 showed the maximum degradation of oxalic acid (23?%) after 60?min of incubation with fungal extract having oxalate oxidase activity. Microscopic observation and ITS (internally transcribed spacers) sequencing categorized the potential fungal strains into the Aspergillus, Fusarium and Trichoderma. Trichoderma sp. are well studied biocontrol agent and interestingly we also found the oxalate oxidase type activity in these strains which further strengthens the potentiality of these biocontrol agents.  相似文献   

19.
When Micrococcus sp. which was isolated from soil assimilated azelaic acid as a sole carbon source, cell-free extract of the organism catalyzed enzymic fatty acid hydroxamate formation. The enzyme was effective only for mono-carboxylic acid, but not for di-carboxylic acids such as azelaic acid. The activity was high with higher fatty acid such as oleic acid. Some of the properties of higher fatty acid hydroxamate formation were investigated.

Olelylhydroxamate was formed with the high concentration of hydroxylamine. The reaction was inhibited by PCMB, but recovered by the addition of SH-compounds (such as cysteine).

On the other hand, when methylacetate was used as a sole carbon source and cell-free extract of Micrococcus sp. hydrolyzed several fatty acid esters. The fatty acid hydroxamate degradation by esterolysis are also discussed.  相似文献   

20.
Summary From the anoxic zone of an oil shale leachate column three pyridine-degrading bacterial strains were isolated. Two strains were Gram-negative facultative anaerobic rods and one strain was a branched Gram-positive bacterium. The branched Gram-positive strain had the best pyridine-degrading ability. This organism was aerobic, non-motile, catalase positive, oxidase negative, and had no flagellum. The G+C content of the DNA was 66.5 mol%. The major menaquinone was MK-8(H2). The main cellular fatty acids were saturated and monounsaturated straight chains. This organism contained mycolic acid, meso-diaminopimelic acid, arabinogalactan and glycolyl residues in the cell wall. Due to morphological, physiological and chemotaxonomic characteristics this strain was placed in the genus Rhodococcus. The optimum culture conditions were as follows: temperature 32° C, pH 8.0 and 0.1% v/v of pyridine as sole carbon, energy and nitrogen source. Utilization of pyridine by a batch fermentor culture of Rhodococcus sp. was characterized by a specific growth rate of 0.13 h–1, growth yield of 0.61 mg cell·mg pyridine–1 and a doubling time of 5.3 h–1. Offprint requests to: S.-T. Lee  相似文献   

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