蜡样芽孢杆菌ATCC14579毒力基因plcR缺失株的构建及其一般特性

蜡样芽孢杆菌是土壤中的优势菌,具有作为益生菌的潜在能力,同时它也是条件致病菌,能引起食物中毒等。蜡样芽孢杆菌的多种毒力因子受到多效性调控子(pleiotropic regulator,plcR)的调控,在其条件致病性作用中起着重要作用。真养产碱杆菌JMP34(Alcaligenes eutrophus)质粒上的2,4-二氯苯氧乙酸(2,4-dichlorophenoxyacetic acid,2,4-D)单加氧酶(tfdA)基因可以降解2,4-D。本研究利用同源重组技术使tfdA基因置换掉蜡样芽孢杆菌的plcR基因,构建了蜡样芽孢杆菌ATCC14579突变株B.cereus△plcRΩtf-dA,并对其毒性、一般生理生化特性进行分析。研究结果表明,突变株B.cereus△plcRΩtfdA的毒性显著减弱;生理生化实验结果显示突变株与野生株并没有明显区别,且突变株并没有表现出tfdA酶活性。所有的结果表明plcR控制着蜡样芽孢杆菌ATCC14579的致病性,同时剔除plcR并不破坏其酶系统。本研究为今后构建蜡样芽孢杆菌工程菌提供了新的思路和依据。     

番茄灰霉病高效拮抗菌株的鉴定及通过导入β-1,3-葡聚糖酶基因提高其生防效果

从淄博市温室土壤中分离到蜡样芽孢杆菌B-04菌株,对灰霉病菌表现较高的拮抗作用。本研究从质粒pUC1940得到4.1kb的β-1,3-葡聚糖酶基因片断,将该基因与大肠杆菌-芽孢杆菌穿梭质粒pBE2和pHY300PLK连接,获得重组质粒PBE2-glu和pHY300PLK-glu,转入蜡样芽孢杆菌（Bacillus cereus）B-04菌株,获得工程菌株B-04-glu。限制酶切分析、ABP平板、PCR实验证实B-04成功转入β-1,3-葡聚糖酶基因。与野生菌株相比,平板拮抗试验表明工程菌株较原始菌株对番茄灰霉病（Botrytis cinerea）抑菌效果明显增强。     

野生黄芩内生真菌的分离鉴定及抗菌活性筛选

从野生黄芩的根、茎、叶中分离出97株内生真菌, 其中33株来自生长期植株, 64株来自成熟期植株。经分类鉴定, 隶属于38个属, 优势属为Alternaria, 成熟期的属种多样性高于生长期。将其中的95株内生真菌对14种指示菌进行抑菌试验, 发现其中91株内生真菌对一种或多种指示菌具有抑菌活性, 占分离菌株总数的95.8%。野生黄芩内生真菌对大肠杆菌CGMCC1.1103、枯草芽孢杆菌CGMCC1.769、蜡样芽孢杆菌ATCC1361、致病性大肠埃希氏菌ATCC49105、白色假丝酵母ATCC10231、小孢拟盘多毛孢CNUMB2PM01等6个指示菌菌株抑菌效果较好; 对单核细胞增生李斯特菌ATCC27708、普通变形杆菌ATCC33420、肠炎沙门氏菌ATCC14208、副溶血型弧菌ATCC27519、宋内氏痢疾杆菌ATCC51060、九州镰孢霉CNUMB2FK01等6个指示菌菌株抑菌效果较差; 对深红酵母CGMCC2.282和金黄色葡萄球菌ATCC12600没有抑制作用。     

动物用芽孢杆菌微生态制剂中蜡样芽孢杆菌的分离及安全性

【背景】芽孢杆菌是仅次于乳酸菌常用于微生态制剂中的菌种,然而部分芽孢杆菌微生态制剂规范不严,应用存在安全隐患。【目的】调查我国在售动物用芽孢杆菌微生态制剂中蜡样芽孢杆菌携带情况,揭示蜡样芽孢杆菌应用的潜在风险。【方法】对微生态制剂预处理,选择性筛选分离蜡样芽孢杆菌,通过全基因组测序测绘细菌毒素基因谱与耐药基因谱,细胞计数试剂盒-8法测定菌株对细胞的毒性,利用微量肉汤稀释法确定菌株耐药值。【结果】从50份微生态制剂产品中筛选分离得到23株蜡样芽孢杆菌群细菌,它们对氨苄西林、林可霉素和泰妙菌素3种抗生素均耐药,主要毒力基因nhe、hbl、cytK、ces的检出率分别为100%、30%、39%和4%,分离株均有溶血性且39%菌株产生热稳定毒素,不同菌株对非洲绿猴肾细胞呈现出不同程度的毒性。【结论】微生态制剂来源的蜡样芽孢杆菌毒性与耐药性严重,携带毒素基因与耐药基因广泛,多株菌株呈高细胞毒性且产生热稳定毒素。芽孢杆菌微生态制剂存在安全性问题,应加强对蜡样芽孢杆菌的质量安全监管力度,规范微生态制剂的市场秩序,杜绝安全隐患。     

蜡样芽孢杆菌CP-1对Cr(Ⅵ)还原效果研究

为了提高蜡样芽孢杆菌CP-1菌株对Cr(Ⅵ)的还原效果,采用单因素和正交试验,通过摇瓶发酵培养,对影响蜡样芽孢杆菌CP-1菌株还原Cr(Ⅵ)的发酵培养基成分和培养条件进行了优化,并研究了最佳发酵条件下的蜡样芽孢杆菌CP-1对Cr(Ⅵ)的还原效果。结果表明,蜡样芽孢杆菌CP-1菌株还原Cr(Ⅵ)的最佳培养基组成为:1%甘露醇, 3%的大豆蛋白胨, 0.05%KCl, 0.1%CuSO4,在此基础上的最佳培养条件为:pH7.0、6%接种量、45℃培养3 d,在此条件下,Cr(Ⅵ)初始浓度为100mg·L^-1时,对Cr(Ⅵ)的还原率达99.75%。在Cr(Ⅵ)污染的土壤中添加蜡样芽孢杆菌CP-190d后,土壤中的Cr(Ⅵ)含量降低55.15%左右。     

枯草芽孢杆菌群β-甘露聚糖酶基因克隆及同源性分析

本文对33株枯草芽孢杆菌群菌株进行β-甘露聚糖酶活性筛选,其中的32株具有β-甘露聚糖酶活性,只有1株无β-甘露聚糖酶活性.通过基因克隆测序的方法获得33株枯草芽孢杆菌群菌株β-甘露聚糖酶基因编码区全序列,对酶基因进行同源性分析并构建系统发育树;在β-甘露聚糖酶基因系统发育树中,33株枯草芽孢杆菌群菌株聚为3个分支,分别是枯草芽孢杆菌分支、地衣芽孢杆菌分支和解淀粉芽孢杆菌分支;枯草芽孢杆菌、地衣芽孢杆菌和解淀粉芽孢杆菌β-甘露聚糖酶基因种内同源性大于91%,而种间同源性为60%69%.     

基于裂解酶与ATP生物发光法特异定量检测炭疽杆菌方法的研究

目的:原核表达炭疽杆菌噬菌体γ裂解酶(PlyG)和萤光素酶(Luc),结合这2种酶建立特异定量检测炭疽杆菌的方法。方法:PCR扩增得到带有His标签的裂解酶基因和萤光素酶基因,构建重组表达载体pET22b-plyG和pET22b-luc,转化至大肠杆菌BL21(DE3)并诱导表达,过镍柱纯化得到目的蛋白;利用裂解酶裂解和ATP生物发光定量检测蜡样芽孢杆菌RSVF1,与平板计数法对比建立线性关系。结果:表达了炭疽杆菌噬菌体γ裂解酶和萤光素酶,并建立了特异定量检测蜡样芽孢杆菌RSVF1的方法,与平板计数方法具有显著的线性相关。结论:因炭疽杆菌与蜡样芽孢杆菌RSVF1均对PlyG具有较强的敏感性,故本研究所建立的将炭疽杆菌噬菌体γ裂解酶与萤光素-萤光素酶系统相结合的检测方法对现场或临床定性定量检测炭疽杆菌提供了理论支持,具有良好的应用前景。     

林麝源蜡样芽孢杆菌SCBCM001株的分离鉴定及全基因组序列分析

【背景】细菌性疾病是林麝规模化养殖的重要制约因素,蜡样芽孢杆菌曾在林麝化脓灶中检出,但是目前对林麝源蜡样芽孢杆菌的研究报道很少。【目的】对分离自病死林麝肝脏中的一株疑似蜡样芽孢杆菌进行分离鉴定和全基因组序列分析,为林麝相关疾病的防治奠定基础。【方法】将病原菌纯化培养后,对病原菌进行生化试验、药敏试验和小鼠致病性试验;并通过第3代单分子测序技术进行全基因组测序,根据测序结果进一步评估物种间的亲缘关系,并进行基因功能注释和遗传进化分析。【结果】该病原菌经平均核苷酸相似度分类和系统发育树分析属于蜡样芽孢杆菌群,生化结果符合蜡样芽孢杆菌的一般特征,将分离菌株命名为SCBCM001。该菌株对小鼠的半数致死量为8.3×107 CFU,对大多数β-内酰胺类、四环素和磺胺异噁唑耐药,对氨基糖苷类、头孢氨苄、头孢哌酮和亚胺培南等药物敏感;全基因组测序结果表明该菌株的染色体大小为5292570bp,GC含量为35.37%,多位点序列分型显示该菌株属于ST427序列类型。在菌株SCBCM001基因组内发现hblA、hblC、hblD、nheA、nheB、clo和cytK等多种毒力因子,同时菌株携带对β-内酰...     

一株产纤维素酶细菌X10-1-2的鉴定

从土壤中筛选出来一株产纤维素酶的细菌,编号X10-1-2.该菌株为革兰氏阳性杆菌,可生成芽孢,好氧.最高生长温度为35～45℃,最低生长温度为10～20℃.在实验室根据其个体形态、菌落形态、生理生化特征作为分类依据,鉴定为蜡样芽孢杆菌(Bacillus cereus).     

一株食源性蜡样芽孢杆菌的分离及其对小鼠肠黏膜免疫相关因子和肠道菌群的影响

蜡样芽孢杆菌(Bacillus cereus)是一种常见的食源性致病菌,误食被其污染的食品会引起呕吐或者腹泻,严重者还会死亡。本研究利用划线培养法从腐败米饭中分离培养出一株蜡样芽孢杆菌。通过药敏试验分析及毒力相关基因PCR扩增对其致病性及耐药性进行分析,并将细菌培养物经过腹腔注射进行小鼠感染试验,检测其对小鼠肠黏膜免疫相关因子和肠道菌群的影响,从而分析该病原菌的致病途径及防治手段。结果显示,本研究所分离的食源性蜡样芽孢杆菌,对诺氟沙星、呋喃妥因、四环素、米诺环素、环丙沙星、大观霉素、克林霉素、红霉素、克拉霉素、氯霉素、左氟沙星和万古霉素敏感,对复方新诺明、苯唑西林和青霉素G具有耐受性。该菌株携带hblA、hblC、hblD、nheA、nheB、nheC和entFM 7种毒力相关基因,可产生致腹泻型毒素。小鼠感染后,出现腹泻,肠黏膜免疫球蛋白和炎性因子表达水平显著上调;肠道菌群检测结果表明,经该蜡样芽孢杆菌感染后,小鼠肠道菌群组成发生变化,标志着机体健康的拟杆菌门(Bacteroidetes)中的uncultured_bacterium_f_Muribaculaceae丰度显著下降,而标志着菌群失调的变形菌门(Proteobacteria)条件致病菌uncultured_bacterium_f_Enterobacteriaceae丰度显著升高,且与IgM和IgG呈显著正相关(P<0.05)。这些研究结果表明,携带致腹泻型毒力相关基因的致病性蜡样芽孢杆菌感染机体后,可以通过影响肠道菌群激活免疫系统。     

维生素E对中华鳖幼鳖生长、肝脏维生素E以及血清皮质醇含量的影响

为探讨维生素E(VE)对中华鳖(Pelodiscus sinensis)幼鳖的生长、肝脏VE和血清皮质醇的影响，通过特定生长率、高压液相色谱法和放免法，我们测定了中华鳖幼鳖的生长、肝脏VE和血清皮质醇含量。发现VE添加量为1000和5000mg／kg的两组，能明显降低中华鳖幼鳖的生长。维生素E添加量为500、1000和5000mg／kg的三组，肝脏维生素E含量明显高于对照组，VE添加量在0—1000mg／kg的范围时，肝脏VE的含量随着饲料中VE含量的增加呈指数式增加，并且在VE添加量为5000kg／kg的一组基本达到饱和。维生素E添加量为0和50mg／kg的2组，其血清皮质醇的平均值明显高于维生素E添加量为250、500、1000和5000mg／kg的4组的平均值。上述结果表明：高剂量的VE降低了中华鳖幼鳖的生长和血清皮质醇的含量；在一定剂量范围内，肝脏VE随着饲料中VE含量的增加而升高。     

枯草芽孢杆菌对草鱼肝脏脂质代谢的调节作用

为研究益生枯草芽孢杆菌(Bacillus subtilis)对草鱼(Ctenopharyngodon idella)肝脏脂质代谢及抗氧化功能的影响, 实验设置对照组(Con组)、Aeromonas hydrophila组(Ah组)、Aeromonas hydrophila+Bacillus subtilis组(Ah+Bs组)、Bacillus subtilis+Aeromonas hydrophila组(Bs+Ah组), 三个实验组均腹腔注射1×105 CFU/fish嗜水气单胞菌(Aeromonas hydrophila), 枯草芽孢杆菌饲料含菌量为1×107 CFU/g, 实验周期为56d, 并于第28和第56天取样。结果表明, 与Ah组相比, 投喂枯草芽孢杆菌饲料后, (1)体增重率、特定生长率显著增加(P<0.05); (2) 28d时肝脏油红O染色脂滴面积及脂肪含量显著下降(P<0.05); (3)调节血脂代谢及缓解肝脏损伤: 血清胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇含量升高, 谷草转氨酶和谷丙转氨酶活性显著降低(P<0.05); (4)调节脂质代谢: 28d时乙酰辅酶A羧化酶的表达水平下调, 脂蛋白脂酶及脂肪甘油三酯脂肪酶的表达水平上调; (5)增强肝脏抗氧化能力、减少脂质过氧化的发生: 肝脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽、总抗氧化能力提高; 丙二醛及过氧化氢含量降低。综上, 在饲料中添加益生枯草芽孢杆菌可以增强草鱼的抗氧化能力, 缓解机体因嗜水气单胞菌感染造成的肝脏损伤, 调节肝脏脂质代谢功能, 减少脂质在肝脏中的积累, 并促进草鱼的生长。     

Fatty acid composition and lipid peroxidation of soft-shelled turtle, Pelodiscus sinensis, fed different dietary lipid sources

Juvenile soft-shelled turtles (Pelodiscus sinensis) were fed 7 diets containing 8% of lard, soybean oil, olive oil, menhaden fish oil, or mixtures of 1 to 1 ratio of fish oil and lard, soybean oil, olive oil for 10 weeks. Growth and muscle proximate compositions of the turtles were not affected by different dietary treatments (p>0.05). Fatty acid profiles in muscle polar lipids, muscle non-polar lipids, and liver polar lipids reflected the fatty acid composition of dietary lipid source. Turtles fed diets containing fish oil generally contained significantly higher (p<0.05) proportion of highly unsaturated fatty acids (HUFA) in both polar and non-polar lipids of muscle and polar fraction of liver lipids than those fed other oils. Non-polar fraction of liver lipids from all groups of turtles contained less than 1% of HUFA. All turtles contained relatively high proportions of oleic acid in their lipids regardless of the dietary lipid source. Further, lipid peroxidation in both muscle tissue and liver microsomes of turtles fed fish oil as the sole lipid source was greater (p<0.05) than those fed fish oil-free diets. Turtles fed olive oil as the sole lipid source had the lowest lipid peroxidation rate among all dietary groups. The results indicate that dietary n-3 HUFA may not be crucial for optimal growth of soft-shelled turtles although they may be used for metabolic purpose. Further, high level of dietary HUFA not only increases the HUFA content in turtle tissues, but also enhances the susceptibility of these tissues to lipid peroxidation.     

The effect of vitamin C on the non-specific immune response of the juvenile soft-shelled turtle (Trionyx sinensis)

The study was conducted to determine the effect of supplementation vitamin C in dietary non-specific immunity in juvenile soft-shelled turtles. The soft-shelled turtles were fed with vitamin C supplementation at dosages of 0, 250, 500, 2500, 5000 and 10000 mg/kg diets, respectively, for 4 weeks. The results showed that there were no differences in the phagocytosis of blood cells, serum bacteriolytic activity and bactericidal activity among soft-shelled turtles fed with vitamin C supplementation in 0-500 mg/kg diets. However, firm indicators were significantly enhanced in soft-shelled turtles fed with vitamin C supplementation at 2500 mg/kg diets compared with those fed at 0 and 250 mg/kg diets. The soft-shelled turtles fed with 5000 mg/kg diets had only significantly higher bactericidal activity than those fed vitamin C-deficient diets. The vitamin C supplementation in 10000 mg/kg diets had no notable effects on the phagocytosis, bacteriolytic activity and bactericidal activity. These results suggest that vitamin C seems have an upper and lower threshold for improving non-specific immune function, and the optimum dose was 2500 mg/kg.     

Dietary lipid level influences fatty acid profiles, tissue composition, and lipid peroxidation of soft-shelled turtle, Pelodiscus sinensis

Dietary lipids containing equal portions of soybean oil and fish oil were fed to juvenile Chinese soft-shelled turtle, Pelodiscus sinensis, at supplementation level of 0 to 15% for 8 weeks. Tissue fat contents of turtles increased when dietary lipid concentration increased. Fatty acid profiles for turtles fed diets supplemented with 6% or higher levels of lipids were similar to those in dietary lipids. On absolute value basis, fatty acids of 14-, 16-, and 18-carbons in muscle of turtles fed diet without lipid supplementation were higher than those in the initial turtle muscle. Among them, C16:1 and C18:1 was approximately 4 and 2 fold higher, respectively, than that of the initial turtles. By contrast, absolute amounts of C20:5 and C22:6 in muscle of turtles fed diet without lipid supplementation were slightly less than those in the initial turtles. For turtles fed lipid supplemented diets, tissue C20:5 and C22:6, however, increased when dietary lipid level increased. These results suggest that soft-shelled turtles are capable of synthesizing fatty acids up to 18 carbons from other nutrients and that they may have limited or no ability to synthesize highly unsaturated fatty acids. Lipid peroxidation measured by thiobarbituric acid-reactive substances in tissues of turtles fed 12% and 15% lipids was greater (p<0.05) than that in turtles fed 3% to 9% lipids. This could be due to high lipid and unsaturated fatty acid content in these tissues. On lipid basis, lipid peroxidation in turtles fed diet without lipid supplementation was the highest among all groups suggesting the existence of antioxidant factors in the dietary lipids.     

The effects of dietary vitamin C on growth,liver vitamin C and serum cortisol in stressed and unstressed juvenile soft-shelled turtles (Pelodiscus sinensis)

We studied the effect of dietary vitamin C on growth, liver vitamin C and serum cortisol levels in stressed and unstressed juvenile soft-shelled turtles. Turtles were fed with vitamin C supplementation at dosages of 0, 250, 500, 2500, 5,000 or 10,000 mg/kg diet for 4 weeks. Vitamin C supplementation exerted significant effects on specific growth rate and liver vitamin C concentrations. The specific growth rate peaked in the group fed at 500 mg/kg diet, while liver vitamin C levels increased with increasing dietary vitamin C levels. Serum cortisol concentrations did not differ between groups of turtles fed diets supplemented with vitamin C in the range of 0-10,000 mg/kg. After acid stress, hepatic vitamin C levels were unaffected, while serum cortisol in the control group was significantly elevated (P<0.01). The other five groups of turtles did not show significant changes in serum cortisol compared with pre-stress levels.     

Effects of diving and swimming behavior on body temperatures of pacific leatherback turtles in tropical seas

Mathematical models and recordings of cloacal temperature suggest that leatherback turtles (Dermochelys coriacea) maintain core body temperature higher than ambient water temperature (T(W)) while freely swimming at sea. We investigated the thermoregulatory capabilities of free-ranging leatherbacks and, specifically, the effect that changes in diving patterns and ambient temperatures have on leatherback body temperatures (T(B)). Data loggers were used to record subcarapace and gastrointestinal tract temperatures (T(SC) and T(GT), respectively), T(W), swim speed, dive depth, and dive times of female leatherback turtles during internesting intervals off the coast of Guanacaste, Costa Rica. Mean T(SC) (28.7 degrees -29.0 degrees C) was significantly higher than mean T(W) (25.0 degrees -27.5 degrees C). There was a significant positive relationship between T(SC) and T(W) and a significant negative correlation between T(SC) and dive depth and T(GT) and dive depth. Rapid fluctuations in T(GT) occurred during the first several days of the internesting interval, which suggests that turtles were ingesting prey or water during this time. Turtles spent 79%-91% of the time at sea swimming at speeds greater than 0.2 m s(-1), and the average swim speed was 0.7 +/- 0.2 m s(-1). Results from this study show that alterations in diving behavior and T(W) affect T(B) of leatherback turtles in the tropics. Body temperatures of free-ranging leatherback turtles correspond well with values for T(B) predicted by mathematical models for tropical conditions.     

鼋骨骼系统及与中华鳖比较

采用虫蚀法制备了鼋Pelochelys cantorii和中华鳖Pelodiscus sinensis的骨骼标本, 对骨骼系统进行了观察、描述、绘图及比较分析。结果显示, 鼋的骨骼共169枚, 由背甲和腹甲组成的外骨骼、中轴骨和附肢骨组成的内骨骼组成。通过比较鼋和中华鳖的骨骼结构, 发现二者在头骨的吻突长度及第三颈椎结构方面有较大差别。鼋眼眶前部至吻突最前端的长度与头骨总长度比为0.082, 而中华鳖为0.570, 显示中华鳖吻突显著长于鼋。鼋与中华鳖的颈椎骨数目均为9枚, 但中华鳖的第三至第九颈椎的横突要更明显, 第七颈椎的椎体向上显著翘起, 且第九颈椎腹面椎体前端为尖状。从整条颈椎上看, 鼋脊椎长度与其背甲长度之比为0.66, 中华鳖为1.07, 表明中华鳖的颈椎更长; 研究结果丰富了鳖科动物的骨骼学基础数据, 也为鼋物种鉴定、龟鳖动物系统演化及生态适应性提供骨骼学理论依据。     

嗜水气单胞菌佐剂灭活苗免疫中华鳖抵抗嗜水气单胞菌的致死性感染

通过建立中华鳖浸泡感染模型评价中华鳖嗜水气单胞菌油乳剂灭活疫苗对中华鳖的免疫保护效力。在主动免疫保护试验中,疫苗免疫组中华鳖能产生较高的抗体水平,在使用10×LD50的嗜水气单胞菌T3株进行浸泡攻毒后,保护率为100%(10/10),生理盐水对照组中华鳖的存活率仅为30%(3/10)。在被动免疫保护试验中,疫苗腹腔免疫异育银鲫抗血清能80%(8/10)保护中华鳖抵抗10×LD50的T3株的腹腔接种的攻击,生理盐水对照组中华鳖的存活率为20%(2/10)。研究结果表明嗜水气单胞菌佐剂油乳剂灭活苗具有良好的免疫学原性,可有效预防由嗜水气单胞菌引起的中华鳖红底板和肠道败血症等疾病。     

罗伊氏乳杆菌对组胺引起黄颡鱼肝肠损伤修复效果评价

以黄颡鱼(Pelteobagrus fulvidraco)(14.23±0.65) g为实验对象, 分别饲喂3组等氮饲料(C组: 组胺含量147 mg/kg; H组: 479 mg/kg和H+B组: 496 mg/kg+1×105 CFU/g罗伊氏乳杆菌Lactobacillus reuteri), 进行56d的养殖实验, 研究组胺及罗伊氏乳杆菌对黄颡鱼的影响。结果显示, H+B组血清谷丙转氨酶(ALT)及谷草转氨酶(AST)活力显著低于H组(P<0.05), 而与C组无差异(P>0.05); H+B组肝脏谷胱甘肽S转移酶(GSH-ST)活力及丙二醛(MDA)含量显著低于H组(P<0.05), 与C组无差异(P>0.05); 肝脏HE染色显示, H组肝脏中有较多炎症细胞浸润, 而H+B组肝脏细胞结构完整, 无明显损伤; 肠道HE染色显示, H组肠道皱襞数量明显减少、长度显著变短, 有水肿发生, 而H+B组肠道皱襞数量、长度与对照组无差异, 水肿程度不明显。结果表明: 组胺会引起黄颡鱼肝脏、肠道损伤, 而罗伊氏乳杆菌对组胺引发的肝肠损伤有保护功效。