Effect of host plants on the infectivity of nucleopolyhedrovirus to Spodoptera exigua larvae

Previous studies have shown that the infectivity of baculovirus to herbivores is affected by phytochemicals ingested during the acquisition of viral inoculum on the foliage of host plants. Here, we measured the effects of 14 host plant species on the infectivity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) to its larvae. The order of the LD₅₀ values of SeNPV among the host plants was Ipomoea aquatica > Brassica oleracea > Raphanus sativus > Amaranthus tricolor > Spinacia oleracea > Vigna unguiculata > Solanum melongena > Capsicum annuum > Apium graveolens > Allium fistulosum > Lactuca sativa > Brassica chinensis > Zea mays > Glycine max, with 940.1 ± 2.26, 424.0 ± 0.60, 295.2 ± 1.13, 147.3 ± 0.63, 138.6 ± 0.22, 119.9 ± 0.07, 119.8 ± 0.02, 109.2 ± 0.18, 104.8 ± 0.62, 102.1 ± 0.66, 97.9 ± 0.22, 89.9 ± 0.32, 79.0 ± 0.13 and 64.0 ± 0.38 OBs per larva, respectively, and the values of mean time to death of virus‐infected larvae were 6.21 ± 0.11, 7.12 ± 0.10, 7.33 ± 0.21, 6.97 ± 0.02, 7.06 ± 0.01, 7.29 ± 0.03, 7.32 ± 0.05, 7.07 ± 0.08, 7.24 ± 0.11, 7.09 ± 0.13, 7.50 ± 0.06, 7.23 ± 0.01, 7.30 ± 0.02 and 7.19 ± 0.07 days, respectively. The mean time to death of larvae decreased with increasing viral dose, and corrected mortality decreased as the larval mean time to death increased. These findings have significance for understanding the effects of host plants on the infectivity of baculovirus to noctuids.     

A phylogenetic analysis and systematic revision of the cryptobranch dorids (Mollusca, Nudibranchia, Anthobranchia)

The phylogenetic relationships of the cryptobranch dorids are studied based on morphological characters of species belonging to all previously described genera. The phylogenetic hypothesis supports the cryptobranch dorids as a monophyletic group. There are two major clades within the Cryptobranchia: the radula‐less dorids (Porostomata), and the radula‐bearing dorids ( Labiostomata new taxon ). Labiostomata consists of those taxa sharing a more recent common ancestor with Actinocyclus than with Mandelia, and includes several monophyletic groups: Actinocyclidae, Chromodorididae, Dorididae and Discodorididae. The traditional group Phanerobranchia is probably paraphyletic. The new classification proposed for the Cryptobranchia addresses concepts of phylogenetic nomenclature, but is in accordance with the rules of the International Code of Zoological Nomenclature. The following genera of cryptobranch dorids are regarded as valid: Doris Linnaeus, 1758, Asteronotus Ehrenberg, 1831, Atagema J. E. Gray, 1850, Jorunna Bergh, 1876, Discodoris Bergh, 1877, Platydoris Bergh, 1877, Thordisa Bergh, 1877, Diaulula Bergh, 1878, Aldisa Bergh, 1878, Rostanga Bergh, 1879, Aphelodoris Bergh, 1879, Halgerda Bergh, 1880, Peltodoris Bergh, 1880, Hoplodoris Bergh, 1880, Paradoris Bergh, 1884, Baptodoris Bergh, 1884, Geitodoris Bergh, 1891, Gargamella Bergh, 1894, Alloiodoris Bergh, 1904, Sclerodoris Eliot, 1904, Otinodoris White, 1948, Taringa Er. Marcus, 1955 , Sebadoris Er. Marcus & Ev. Marcus, 1960, Conualevia Collier & Farmer, 1964, Thorybopus Bouchet, 1977, Goslineria Valdés, 2001, Pharodoris Valdés, 2001, Nophodoris Valdés & Gosliner, 2001. Several genera previously considered as valid are here regarded as synonyms of other names: Doridigitata d’Orbigny, 1839, Doriopsis Pease, 1860, Staurodoris Bergh, 1878, Fracassa Bergh, 1878, Archidoris Bergh, 1878, Anoplodoris Fischer, 1883, Etidoris Ihering, 1886, Phialodoris Bergh, 1889, Montereina MacFarland, 1905, Ctenodoris Eliot, 1907, Carryodoris Vayssière, 1919, Austrodoris Odhner, 1926, Guyonia Risbec, 1928, Erythrodoris Pruvot‐Fol, 1933, Neodoris Baba, 1938, Siraius Er. Marcus, 1955, Tayuva Ev. Marcus & Er. Marcus, 1967, Nuvuca Ev. Marcus & Er. Marcus, 1967, Doriorbis Kay & Young, 1969, Pupsikus Er. Marcus & Ev. Marcus, 1970, Percunas Ev. Marcus, 1970, Verrillia Ortea & Ballesteros, 1981 . The genera Artachaea Bergh, 1882, Carminodoris Bergh, 1889 and Homoiodoris Bergh, 1882 have been poorly described and no type material is known to exist. They are regarded as incertae sedis until more material becomes available. The genus names Xenodoris Odhner in Franc, 1968 and Cryptodoris Ostergaard, 1950 are unavailable within the meaning of the Code. Hexabranchus Ehrenberg, 1831 is not a cryptobranch dorid, as suggested by other authors, because of the lack of a retractile gill. Other nomenclatural and taxonomic problems are discussed, and several type species, neotypes and lectotypes are selected. © 2002 The Linnean Society of London. Zoological Journal of the Linnean Society, 2002, 136 , 535?636.     

全球陆地生态系统光合作用与呼吸作用的温度敏感性

基于全球647套通量数据,定量分析了全球尺度下生态系统光合作用和呼吸作用的温度敏感性(Q10)随纬度、气候和植被的分布规律。结果表明:在全球尺度下,光合作用和呼吸过程的温度敏感性(Q10,G和Q10,R)都随纬度的升高而增加,其中Q10,G和Q10,R的均值分别为3.99±0.21和2.28±0.074。除热带多树草原、常绿落叶林外,Q10,G均大于Q10,R值。不同植被类型的温度敏感性存在显著性差异,表现为:针叶林阔叶林;落叶林常绿林,其中生态系统的季节性变异是造成差异的主要原因。当植被类型和纬度区域共同影响Q10值时,植被类型对Q10值的总变异贡献更大。气候类型对Q10,G和Q10,R都有显著影响。在气候带上,干旱带的Q10,G最小,而冷温带的Q10,G最高。不同气候类型下(除温带草原气候外)的Q10,G都大于Q10,R。在极端条件下,温度可能不在是主导因素,而水分对温度敏感性的影响不可忽略,今后的研究需要更多的关注生态系统温度敏感性对水分变化的响应。     

Family-group names in Coleoptera (Insecta)

We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant) based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification.New synonymies (family-group names followed by genus-group names): Agronomina Gistel, 1848 syn. nov. of Amarina Zimmermann, 1832 (Carabidae), Hylepnigalioini Gistel, 1856 syn. nov. of Melandryini Leach, 1815 (Melandryidae), Polycystophoridae Gistel, 1856 syn. nov. of Malachiinae Fleming, 1821 (Melyridae), Sclerasteinae Gistel, 1856 syn. nov. of Ptilininae Shuckard, 1839 (Ptinidae), Phloeonomini Ádám, 2001 syn. nov. of Omaliini MacLeay, 1825 (Staphylinidae), Sepedophilini Ádám, 2001 syn. nov. of Tachyporini MacLeay, 1825 (Staphylinidae), Phibalini Gistel, 1856 syn. nov. of Cteniopodini Solier, 1835 (Tenebrionidae); Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein) syn. nov. of Amara Bonelli, 1810 (Carabidae), Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy) syn. nov. of Melandrya Fabricius, 1801 (Melandryidae), Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein) syn. nov. of Malachius Fabricius, 1775 (Melyridae), Sclerastes Gistel, 1856 (type species Ptilinus costatus Gyllenhal, 1827, designated herein) syn. nov. of Ptilinus Geoffroy, 1762 (Ptinidae), Paniscus Gistel, 1848 (type species Scarabaeus fasciatus Linnaeus, 1758, designated herein) syn. nov. of Trichius Fabricius, 1775 (Scarabaeidae), Phibalus Gistel, 1856 (type species Chrysomela pubescens Linnaeus, 1758, by monotypy) syn. nov. of Omophlus Dejean, 1834 (Tenebrionidae). The following new replacement name is proposed: Gompeliina Bouchard, 2011 nom. nov. for Olotelina Báguena Corella, 1948 (Aderidae).Reversal of Precedence (Article 23.9) is used to conserve usage of the following names (family-group names followed by genus-group names): Perigonini Horn, 1881 nom. protectum over Trechicini Bates, 1873 nom. oblitum (Carabidae), Anisodactylina Lacordaire, 1854 nom. protectum over Eurytrichina LeConte, 1848 nom. oblitum (Carabidae), Smicronychini Seidlitz, 1891 nom. protectum over Desmorini LeConte, 1876 nom. oblitum (Curculionidae), Bagoinae Thomson, 1859 nom. protectum over Lyprinae Gistel 1848 nom. oblitum (Curculionidae), Aterpina Lacordaire, 1863 nom. protectum over Heliomenina Gistel, 1848 nom. oblitum (Curculionidae), Naupactini Gistel, 1848 nom. protectum over Iphiini Schönherr, 1823 nom. oblitum (Curculionidae), Cleonini Schönherr, 1826 nom. protectum over Geomorini Schönherr, 1823 nom. oblitum (Curculionidae), Magdalidini Pascoe, 1870 nom. protectum over Scardamyctini Gistel, 1848 nom. oblitum (Curculionidae), Agrypninae/-ini Candèze, 1857 nom. protecta over Adelocerinae/-ini Gistel, 1848 nom. oblita and Pangaurinae/-ini Gistel, 1856 nom. oblita (Elateridae), Prosternini Gistel, 1856 nom. protectum over Diacanthini Gistel, 1848 nom. oblitum (Elateridae), Calopodinae Costa, 1852 nom. protectum over Sparedrinae Gistel, 1848 nom. oblitum (Oedemeridae), Adesmiini Lacordaire, 1859 nom. protectum over Macropodini Agassiz, 1846 nom. oblitum (Tenebrionidae), Bolitophagini Kirby, 1837 nom. protectum over Eledonini Billberg, 1820 nom. oblitum (Tenebrionidae), Throscidae Laporte, 1840 nom. protectum over Stereolidae Rafinesque, 1815 nom. oblitum (Throscidae) and Lophocaterini Crowson, 1964 over Lycoptini Casey, 1890 nom. oblitum (Trogossitidae); Monotoma Herbst, 1799 nom. protectum over Monotoma Panzer, 1792 nom. oblitum (Monotomidae); Pediacus Shuckard, 1839 nom. protectum over Biophloeus Dejean, 1835 nom. oblitum (Cucujidae), Pachypus Dejean, 1821 nom. protectum over Pachypus Billberg, 1820 nom. oblitum (Scarabaeidae), Sparrmannia Laporte, 1840 nom. protectum over Leocaeta Dejean, 1833 nom. oblitum and Cephalotrichia Hope, 1837 nom. oblitum (Scarabaeidae).     

Toxic and behavioural effects of free fatty acids on western corn rootworm (Coleoptera: Chrysomelidae) larvae

Feeding behaviour, feeding intensity and staying behaviour of neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae were evaluated in response to synthetic feeding stimulant blends. All of the treatments contained a 3‐sugar blend (glucose : fructose : sucrose, 30 : 4 : 4 mg/ml) and one of twelve free fatty acids. Each free fatty acid was tested in this blend at three different concentrations. The addition of the 12 : 0, 16 : 0, 16 : 1, 18 : 0, 18 : 1, 18 : 2 and 18 : 3 free fatty acids to the sugar blend significantly (P < 0.05) increased the percentage of larvae feeding, but did not increase food consumption per larva. Most of the free fatty acids elicited staying behaviour. At the lowest dose (0.1 mg/ml), all of the free fatty acids except the 18 : 0 and the 20 : 0 elicited staying by significantly more larvae than the sugar blend, and at the highest dose (1.0 mg/ml), eight free fatty acids (8 : 0, 10 : 0, 12 : 0, 14 : 0, 16 : 1, 18 : 1, 18 : 2 and 18 : 3) caused more larvae to stay compared to the sugar blend. Larvae were visibly impaired after exposure to some of the free fatty acids. At the highest dose, the 8 : 0, 10 : 0, 12 : 0, 14 : 0, 16 : 1, 18 : 1 and 18 : 2 free fatty acids were toxic to the larvae. At least 60% of larvae were impaired after exposure to the 12 : 0, 16 : 1 and 18 : 2 free fatty acids and the 8 : 0 and 10 : 0 free fatty acids caused 100% impairment or death. Synthetic blends were compared with liquid from crushed maize roots and with a methanol extract of maize roots. Feeding intensity and staying behaviour on the root liquid and the root extract were significantly greater than on any of the synthetic blends, suggesting the presence of additional compounds in maize roots that serve as feeding cues for western corn rootworm larvae.     

Physiological differences in the growth of <Emphasis Type="Italic">Sargassum horneri</Emphasis> between the germling and adult stages

The effects of temperature, irradiance, and daylength on Sargassum horneri growth were examined at the germling and adult stages to discern their physiological differences. Temperature–irradiance (10, 15, 20, 25, 30°C × 20, 40, 80 μmol photons m⁻²s⁻¹) and daylength (8, 12, 16, 24 h) experiments were carried out. The germlings and blades of S. horneri grew over a wide range of temperatures (10–25°C), irradiances (20–80 μmol photons m⁻²s⁻¹), and daylengths (8–24 h). At the optimal growth conditions, the relative growth rates (RGR) of the germlings were 21% day⁻¹ (25°C, 20 μmol photons m⁻²s⁻¹) and 13% day⁻¹ (8 h daylength). In contrast, the RGRs of the blade weights were 4% day⁻¹ (15°C, 20 μmol photons m⁻²s⁻¹) and 5% day⁻¹ (12 h daylength). Negative growth rates were found at 20 μmol photons m⁻²s⁻¹ of 20°C and 25°C treatments after 12 days. This phenomenon coincides with the necrosis of S. horneri blades in field populations. In conclusion, we found physiological differences between S. horneri germlings and adults with respect to daylength and temperature optima. The growth of S. horneri germlings could be enhanced at 25°C, 20 μmol photons m⁻²s⁻¹, and 8 h daylength for construction of Sargassum beds and restoration of barren areas.     

Ethylenesulfide as a useful agent for incorporation into the biopolymer chitosan in a solvent-free reaction for use in cation removal

Chitosan (Ch) was chemically modified with ethylenesulfide (Es) under solvent-free conditions to give (ChEs), displaying a high content of thiol groups due to opening of the three member cyclic reagent. Elemental analysis showed a decrease in nitrogen content. This result indicated the incorporation of two ethylenesulfide molecules for each unit of the polymeric structure of the precursor biopolymer. Infrared spectroscopy, thermogravimetry, and ¹³C NMR in the solid state demonstrated the effectiveness of the reaction, with signals at 30 ppm for ChEs due to the change in the methylene group environment. Divalent metal uptake by chemically modified biopolymer gave the order Cu > Ni > Co > Zn, reflecting the corresponding acidity of these cations in bonding to the sulfur and the basic nitrogen atoms available on the pendant chains. The equilibrium data were fitted to Freundlich, Temkin, and Langmuir models. The maximum monolayer adsorption capacity for the cations was found to be 1.54 ± 0.02, 1.25 ± 0.03, 1.13 ± 0.01, and 0.83 ± 0.03 mmol g⁻¹, respectively. The Langmuir model best explained the cation–sulfur bond interactions at the solid–liquid interface. The thermodynamics for these interactions gave exothermic enthalpic values of −43.02 ± 0.03, −28.72 ± 0.02, −26.27 ± 0.04, and −17.32 ± 0.02 kJ mol⁻¹, respectively. The spontaneity of the systems is given by negative Gibbs free energies of −31.2 ± 0.1, −32.7 ± 0.1, −31.7 ± 0.1, and −32.2 ± 0.1 kJ mol⁻¹, respectively, in spite of the unfavorable negative entropic values of −39 ± 1, −13 ± 1, −18 ± 1, and −49 ± 1 J K⁻¹ mol⁻¹ due to solvent ordering in the course of complexation. This newly synthesized biopolymer is presented as a chemically useful material for cation removal from aqueous solution.     

Does immunization of cucumber against anthracnose by Colletotrichum lagenarium affect host suitability for arthropods?

Restricted infection of a lower leaf of cucumber,Cucumis sativus L., with the anthracnose fungusColletotrichum lagenarium has been previously shown by others to induce persistent, systemic resistance to the same fungus and to at least 12 other diverse plant pathogens. The non-specificity of pathogen-induced resistance has fueled speculation that it might also affect arthropod herbivores. However, we found that immunization of cucumber withC. lagenarium had no effect on population growth of twospotted spider mites,Tetranychus urticae Koch, reared on foliage for which induced resistance to the same pathogen was confirmed. Similarly, immunization withC. lagenarium had no systemic effect on weight gain, duration of development, or pupal weight of fall armyworms, or on progeny production by melon aphids. In reciprocal tests, previous feeding injury from spider mites or fall armyworms did not induce systemic resistance toC. lagenarium. These results indicate that, at least for cucumber, pathogen-activated induced resistance is specific to plant pathogens, suggesting separate mechanisms of induced resistance to pathogens or herbivores.

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Résumé Dans une étude de résistance induite, l'infection basale des feuilles du concombre,Cucumis sativus L., avecColletotrichum lagenarium, le champignon de l'anthracnose, a généré une induction systémique et persistante, non seulement au même champignon, mais aussi et surtout à l'égard de douze autres. La nature non-spécifique de cette résistance a dès lors engendré une hypothèse, celle de savoir si cette non-spécificité pourrait s'entendre au niveau d'arthropodes phytophages. Cependant, nos travaux ont démontré que l'immunité vis-à-vis deC. lagenarium n'affecte ni la population de tétraniques,Tetranychus urticae Koch, élevée sur des feuilles résistantes de concombre; ni le gain pondéral, ni la durée de développement, ni le poids nymphal deSpodoptera frugiperda, ou la fertilité des aphides de melon. De ces résultats, il peut-être déduit que, au moins chez le concombre, l'induction de résistance due àC. lagenarium démeure spécifique aux champignons saprophytes, c'est à dire qu'il existe des mécanismes séparés pour la résistance, soit aux champignons, soit aux arthropodes phytophages.

     

A food plant specialist in Sparganothini: A new genus and species from Costa Rica (Lepidoptera,Tortricidae)

Sparganocosma docsturnerorum Brown, new genus and new species, is described and illustrated from Área de Conservación (ACG) in northwestern Costa Rica. The new genus shares a long, crescent- or ribbon-shaped signum in the corpus bursae of the female genitalia with Aesiocopa Zeller, 1877, Amorbia Clemens, 1860, Amorbimorpha Kruse, 2011, Coelostathma Clemens, 1860, Lambertiodes Diakonoff, 1959, Paramorbia Powell & Lambert, 1986, Rhynchophyllus Meyrick, 1932, Sparganopseustis Powell & Lambert, 1986, Sparganothina Powell, 1986, and Sparganothoides Lambert & Powell, 1986. Putative autapomorphies for Sparganocosma include the extremely short uncus; the smooth (unspined) transtilla; and the upturned, free, distal rod of the sacculus. Adults of Sparganocosma docsturnerorum have been reared numerous times (>50) from larvae collected feeding on rain forest Asplundia utilis (Oerst.) Harling and Asplundia microphylla (Oerst.) Harling (Cyclanthaceae) at intermediate elevations (375–500 m) in ACG. Whereas most Sparganothini are generalists, typically feeding on two or more plant families, Sparganocosma docsturnerorum appears to be a specialist on Asplundia, at least in ACG. The solitary parasitoid wasp Sphelodon wardae Godoy & Gauld (Ichneumonidae; Banchinae) has been reared only from the larvae of Sparganocosma docsturnerorum.     

A taxonomic review of Aramides cajaneus (Aves,Gruiformes, Rallidae) with notes on morphological variation in other species of the genus

The taxonomy of the polytypic and wide-ranging Gray-necked Wood-rail, Aramides cajaneus is reviewed, based on external morphology and voice. Throughout its distribution, there is extensive plumage variation, much of it taxonomically uninformative. However, through three informative plumage characters, as well as morphometric and vocal variation, three phylogenetic species were identified within what is today known as Aramides cajaneus, all of which already had available names: Aramides albiventris Lawrence, 1868, from southern Mexico to northeastern Costa Rica, Aramides cajaneus (Statius Müller, 1776) (sensu stricto), from southwestern Costa Rica to Argentina, and Aramides avicenniae Stotz, 1992, from a small section of the coast of southeastern Brazil. Aramides albiventris presents extensive plumage variation, but with no geographic structure. The song of Aramides cajaneus and Aramides avicenniae is strikingly and completely different from the song of Aramides albiventris. A previously unnoticed parapatric pattern of distribution of Aramides cajaneus and its congener Aramides saracura in southeastern Brazil is described, and we clarify that the name Aramides plumbeicollis, included in the synonymy of Aramides albiventris, was first made available in 1892, rather than in 1888 as is widely referred. In addition, plumage variation in Aramides ypecaha, Aramides wolfi, and Aramides mangle is discussed.