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1.
S Krisa P W Téguo A Decendit G Deffieux J Vercauteren J M Mérillon 《Phytochemistry》1999,51(5):651-656
The use of plant cell cultures for producing isotopically (13C) labelled phenolic substances is reported. Vitis vinifera cells synthesize high levels of anthocyanins when they are cultured in a polyphenol synthesis-inducing medium. Three major anthocyanin monoglucosides found in red wine were identified in grape cells: cyanidin-3-O-beta-glucoside, peonidin-3-O-beta-glucoside, and malvidin-3-O-beta-glucoside. Kinetic study of the intracellular level of phenylalanine and its metabolites showed that it is preferable to add this precursor to grape cell suspensions after the 5th day of culture, i.e. at the beginning of the exponential growth phase. After adding phenylalanine to the culture medium, its uptake was complete and the accumulation of anthocyanins in grape cells was stimulated. Incorporation of [1-13C]-phenylalanine into anthocyanins was measured by means of 13C satellites in the proton NMR spectrum. The maximal rate of 13C enrichment anthocyanins obtained with this technique reached 65%. The production of 13C labelled phenolic compounds was undertaken in order to investigate their absorption and metabolism in humans. 相似文献
2.
Carbon-14 biolabelling of wine polyphenols in Vitis vinifera cell suspension cultures 总被引:1,自引:0,他引:1
Vitrac X Krisa S Decendit A Vercauteren J Nührich A Monti JP Deffieux G Mérillon JM 《Journal of biotechnology》2002,95(1):49-56
14C-L-phenylalanine is incorporated into a range of polyphenolic compounds when fed to grape cell cultures. Optimisation of several parameters such as the quantity of precursor applied and the duration of metabolism led to incorporation yields of 15% and to specific activities of 875 mu Ci g(-1) in stilbenes. Purification of the products by several chromatographic steps is reported. Both trans- and cis-resveratrols were easily obtained by enzymatic hydrolysis of their corresponding glucosides, with specific activity of 1200-1400 mu Ci g(-1). The specific radioactivity obtained for all the compounds is suitable for in vivo feeding trials to trace their metabolic fate when consumed by animals and for in vitro activity mechanism studies. Indeed, these polyphenols seem to be implicated in the health benefits associated with regular and moderate wine consumption but little is known about their pharmacokinetics and cellular uptake. 相似文献
3.
Alain Decendit Kishan Gopal Ramawat Pierre Waffo Gérard Deffieux Alain Badoc Jean-Michel Mérillon 《Biotechnology letters》1996,18(6):659-662
Summary Suspension cultures of Vitis vinifera in a stirred fermenter showed characteristics of growth and polyphenol metabolism similar to that found in shake flasks. In the induction medium, the cells produced mainly anthocyanins (1200 mg/l), proanthocyanidins (220 mg/l), catechins (8 mg/l) and trans-piceid (30 mg/l). 相似文献
4.
葡萄细胞悬浮培养生产白藜芦醇 总被引:1,自引:0,他引:1
以巨峰葡萄果皮为外植体,在添加2.0 mg/L 6-苄基嘌呤(6-BA)和0.1 mg/L 2,4-二氯苯氧基(2,4-D)的B5培养基上诱导葡萄愈伤组织; 以50 g/L的初始接种量在添加1.0 mg/L 6-BA和0.05 mg/L 2,4-D的B5液体培养基上建立葡萄悬浮培养体系。在25~27 ℃下,摇床振荡暗培养(120~130 r/min)18 d后,葡萄细胞生物量和白藜芦醇含量达到最大值(16.17 g/L、95.69 μg/g干质量)。在培养第12天时,向培养基中添加100 μmol/L茉莉酸甲酯(MeJA),经过6 d处理,细胞中白藜芦醇含量达235.73 μg/g干质量。 相似文献
5.
Suspension cultures of Vitis vinifera were cultured in different media in order to establish a model system for promoting high levels of phenolic substances identical with those found in wine. These media were: a low sucrose maintenance medium (MM) and four high sucrose media (differing mainly in sucrose and mineral contents) which were shown to induce secondary metabolism. In MM medium, polyphenol accumulation in the cells was low, and concentrations of 0.1 mg/gfw for condensed tannins and 0.3 mg/gfw for anthocyanins were reached within two weeks of cultivation. Values of 1.4 and 6.4 mg/gfw, respectively, were obtained with a low nitrate and high sucrose medium (HM1), but cell proliferation was reduced. To obtain a maximal production of polyphenols, we investigated the most effective conditions for cell growth and polyphenol production (a high mineral and high sucrose medium, IM1; inoculum dilution of 1.25:10). Under these conditions, the cells produced mainly anthocyanins (1100 mg/l), proanthocyanidins (300 mg/l) and catechins (25 mg/l).Abbreviations BuOH
n-butanol
- dw
dry weight
- fw
fresh weight 相似文献
6.
Decendit A Waffo-Teguo P Richard T Krisa S Vercauteren J Monti JP Deffieux G Mérillon JM 《Phytochemistry》2002,60(8):795-798
Suspension cultures of Vitis vinifera were found to produce catechins and stilbenes. When cells were grown in a medium inducing polyphenol synthesis, (−)-epicatechin-3-O-gallate, dimeric procyanidin B-2 3′-O-gallate and two resveratrol diglucosides were isolated, together with a new natural compound that was identified as cis-resveratrol-3,4′-O-β-diglucoside by spectroscopical methods. 相似文献
7.
This paper is concerned with the potential use of a reciprocating plate bioreactor (RPB) for suspended plant cell cultures. The agitation mechanism of the RPB system, a plate stack, was first evaluated in pure water and in pseudocells medium of 20, 40 and 60% of PCV. As the pseudocell concentration increases, the oxygen mass transfer coefficient, KLa, significantly decreases. Correlations were established for each plate stack and concentration with good prediction of KLa. Three fermentations were performed with Vitis vinifera cells, two in the RPB system and one in shake flasks. Shake flask cultures showed better performance whereas the first fermentation performed with the RPB showed the lowest performance. The lower growth observed was attributed to the operating conditions for aeration and the dissolved oxygen control strategy. CO2 stripping in the initial portion of the fermentation led to lower biomass growth. The second fermentation, with more appropriate operating conditions, appears to follow the trend of shake flask cultures but was terminated after 5 days due to contamination. The RPB has the potential to be used for suspended plant cell cultures but significant research needs to be performed to find optimal operating conditions but, more importantly, to make appropriate modifications to ensure the sterility of the bioreactor over long time periods. 相似文献
8.
Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars 总被引:5,自引:0,他引:5
F. Larronde S. Krisa A. Decendit C. Chèze G. Deffieux J. M. Mérillon 《Plant cell reports》1998,17(12):946-950
Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 m sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars
were required for the induction of polyphenol accumulation. Indeed, the polyols, mannitol and sorbitol, had no effect on this
accumulation. We established a model system to investigate the mechanism of sucrose regulation of polyphenol production without
inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells
were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with
the increase in anthocyanin accumulation in grape cells.
Received: 18 August 1997 / Revision received: 6 November 1997 / Accepted: 5 January 1998 相似文献
9.
Anthocyanic vacuolar inclusions (AVIs) are intra-vacuolar structures capable of concentrating anthocyanins and are present
in over 50 of the highest anthocyanin-accumulating plant species. Presence of AVIs alters pigment intensity, total anthocyanin
levels, pigment hue and causes bathochromic shifts in a spatio-temporal manner within various flowers, vegetables and fruits.
A year-long study on Vitis vinifera cell suspension cultures found a strong correlation between AVI prevalence and anthocyanin content, but not the number of
pigmented cells, growth rate or stilbene content. Furthermore, enhancement of the prevalence of AVIs and anthocyanins was
achieved by treatment of V. vinifera cell suspension cultures with sucrose, jasmonic acid and white light. A unique autofluorescence of anthocyanins was used
to demonstrate microscopically that AVIs proceed from the cytosol across the tonoplast and were able to coalesce intravacuolarly,
with fewer, larger AVIs predominating as cells mature. Purification and characterisation of these bodies were performed, showing
that they were dense, highly organic structures, with a lipid component indicative of membrane-encasement. These purified
AVIs were also shown to comprise long-chain tannins and possessed an increased affinity for binding acylated anthocyanins,
though no unique protein component was detected. 相似文献
10.
Tassoni A Fornalè S Franceschetti M Musiani F Michael AJ Perry B Bagni N 《The New phytologist》2005,166(3):895-905
Here the effect of jasmonic acid, methyljasmonate and Na-orthovanadate on the production of resveratrol was studied in Vitis vinifera cv. Barbera cell suspension cultures. Na-orthovanadate at 0.1 mm and 1 mm concentration was efficient in promoting the production and/or accumulation and release in the culture medium of cis-resveratrol while trans-resveratrol levels were not affected by this treatment. Methyljasmonate was highly effective in stimulating both trans- and cis-resveratrol endogenous accumulation, as well as their release into the culture medium. Cis-resveratrol was absent or detected in very low amounts in the controls. Jasmonic acid was less efficient than methyljasmonate in promoting endogenous resveratrol accumulation, but it stimulated the release in the culture medium especially of cis-resveratrol. Gel analysis was performed on control and 10 microm MeJA treated cell suspensions. Results showed an up-regulation of the stilbene synthase demonstrating that MeJA stimulated the synthesis ex-novo of this protein. 相似文献
11.
Yongmei Che Zhen Zhang Dan Zhu Jie Hao Lixia Hou Xin Liu 《Plant Cell, Tissue and Organ Culture》2019,137(3):455-464
Plant Cell, Tissue and Organ Culture (PCTOC) - Mineral nutrition is a very important factor in the success of in vitro plant cultures. The aim was to compare the predictive capacity of the models... 相似文献
12.
Emine Sema Cetin Zehra Babalik Filiz Hallac-Turk Nilgun Gokturk-Baydar 《Biological research》2014,47(1)
Background
Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell suspension cultures of grapevine. Cell suspensions initiated from callus belonging to petiole tissue was used as a plant material. Cadmium chloride was applied to cell suspension cultures in different concentration (1.0 mM and 1.5 mM) to enhance secondary metabolite (total phenolics, total flavanols, total flavonols, trans-resveratrol, and α-, β-, γ- δ-tocopherols) production. Cells were harvested at two days intervals until the 6th day of cultures. Amounts of total phenolics, total flavanols and total flavonols; trans-resveratrol and tocopherols (α-, β-, γ- and δ-tocopherols) and dry cell weights were determined in the harvested cells.Results
Phenolic contents were significantly affected by the sampling time and cadmium concentrations. The highest values of total phenolic (168.82 mg/100 g), total flavanol (15.94 mg/100 g), total flavonol (14.73 mg/100 g) and trans-resveratrol (490.76 μg/100 g) were found in cells treated with 1.0 mM CdCl2 and harvested at day 2. Contents of tocopherols in the cells cultured in the presence of 1.0 mM CdCl2 gradually increased during the culture period and the highest values of α, β and γ tocopherols (145.61, 25.52 and 18.56 μg/100 g) were detected in the cell cultures collected at day 6.Conclusions
As a conclusion, secondary metabolite contents were increased by cadmium chloride application and sampling time, while dry cell weights was reduced by cadmium chloride treatments. 相似文献13.
14.
Wang Lihua An Mengjiao Huang Weidong Zhan Jicheng 《Plant Cell, Tissue and Organ Culture》2019,138(3):475-488
Plant Cell, Tissue and Organ Culture (PCTOC) - Temperature and copper stress are two common abiotic stresses in viticulture. To better understand biosynthesis of melatonin and phenolics in response... 相似文献
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16.
The course of berry growth in Vitis vinifera has been interpreteddifferently by various authors. Divisions into two, three orfour phases have been postulated, though, in the latter cases,without any objective criteria for their delimitation. To clarifythis point, investigations were carried out with the cultivarsBacchus and Madeleine. Fresh and dry wt curves showed a double sigmoid course and threetransition points could be clearly defined. The central transitionpoint, occurring around 42 d after anthesis, may be definedas the change-over from the first to the second growth phase.Both the course of the fresh weight curve when plotted logarithmicallyand the relative growth rates argue against there being a phaseof slow growth at the beginning of the first growth phase. Indeed,the relative increase in fresh weight is maximal at the beginningof the first growth phase. The delimitation of a separate phase of little or no growthin the region of the transition from the first to the secondgrowth phase is not supported. The definitions of such phaseboundaries is arbitrary. The smaller the number of seeds perberry, the earlier is the first growth phase ended and the secondgrowth phase, including veraison, begun. During the first growth phase there is high cell division activity.The average area of an epidermal cell reached its minimum at811 d after anthesis, with a simultaneous strong increasein cell number. The maximal number of epidermal cells was attainedtowards the end of the first growth phase. The growth of the embryo showed no relation to the double-sigmoidalgrowth of the pericarp. Final embryo size was reached at 7075d after anthesis. Seed d. wt, on the other hand, showed a biphasicincrease. The results presented here support the division of berry growthof V. vinifera into just two phases. Vitis vinifera L., grape vine, berry growth, growth phases 相似文献
17.
18.
François Cormier Chi Bao Do Yves Nicolas 《In vitro cellular & developmental biology. Plant》1994,30(3):171-173
Summary Anthocyanin production of two lines ofVitis vinifera cell cultures, i.e., 5.4 and 13.1, which were obtained from the same starting material after 20 and 37 mo. of clonal selection,
respectively, was investigated. Cell suspension cultures of lines 5.4 and 13.1 maintained an anthocyanin content of 0.44 ±
0.15 and 1.02 ± 0.31 mg·g−1 fresh weight during 50 and 32 weekly maintenance subcultures, respectively. Under anthocyanin-promoting culture conditions,
both lines showed an enhancement of their anthocyanin level by approximately fourfold. While line 5.4 accumulated peonidin
3-glucoside and cyanidin 3-glucoside in decreasing order, line 13.1 accumulated primarily peonidin 3-p-coumaroylglucoside with lesser amounts of malvidin monoglucoside. Results show that while the anthocyanin content was improved
during the course of repeated selections, the anthocyanin composition was modified markedly favoring the accumulation of more
metabolically-advanced anthocyanins. 相似文献
19.
20.
Molecular control mechanisms for abiotic stress tolerance are based on the activation and regulation of specific stress-related
genes. The phytohormone abscisic acid (ABA) is a key endogenous messenger in a plant’s response to such stresses. A novel
ABA binding mechanism which plays a key role in plant cell signaling cascades has recently been uncovered. In the absence
of ABA, a type 2C protein phosphatase (PP2C) interacts and inhibits the kinase SnRK2. Binding of ABA to the PYR/PYLs receptors
enables interaction between the ABA receptor and the PP2C protein, and abrogates the SnRK2 inactivation. The active SnRK2
is then free to activate the ABA-responsive element Binding Factors which target ABA-dependent gene expression. We used the
grape as a model to study the ABA perception mechanism in fruit trees. The grape ABA signaling cascade consists of at least
seven ABA receptors and six PP2Cs. We used a yeast two-hybrid system to examine physical interaction in vitro between the
grape ABA receptors and their interacting partners, and found that twenty-two receptor-PP2C interactions can occur. Moreover,
quantifying these affinities by the use of the LacZ reporter enables us to show that VvPP2C4 and VvPP2C9 are the major binding partners of the ABA receptor. We also tested in vivo the root and leaf gene expression of the various
ABA receptors and PP2Cs in the presence of exogenic ABA and under different abiotic stresses such as high salt concentration,
cold and drought, and found that many of these genes are regulated by such abiotic environmental factors. Our results indicate
organ specificity in the ABA receptor genes and stress specificity in the VvPP2Cs. We suggest that VvPP2C4 is the major PP2C involved in ABA perception in leaves and roots, and VvRCAR6 and VvRCAR5 respectively, are the major receptors involved in ABA perception in these organs. Identification, characterization and manipulation
of the central players in the ABA signaling cascades in fruit trees is likely to prove essential for improving their performance
in the future. 相似文献