南京地区落叶栎林主要木本植物的展叶动态研究

 植物的展叶过程是由自身遗传因子决定的,同时又受到多种生态因子的调节,反映了植物的生活史对策和群落物种多样性的维持机制。在2001和2002年的3～6月间,不定期记录了南京地区三个落叶栎(Quercus spp.)林中主要木本植物的展叶情况,包括被标记标准枝的叶数、叶的长度、宽度、叶面积、叶干重等参数。结果表明在所调查的落叶栎林中,林冠层物种的成熟叶面积和单位叶面积干重都显著大于林下层物种;最早展叶的物种为林下层物种,但林冠层展叶顺序与林下层无显著差异。叶面积越大、单位叶面积干重越小的物种展叶越晚;林冠层物种展叶较林下层快,物种成熟叶面积越大,展叶速率越大。最后对展叶顺序和展叶速度的生态学意义作了讨论。     

Optimal Nitrogen Content and Photosynthesis in Cauliflower (Brassica oleracea L. botrytis). Scaling up from a Leaf to the Whole Plant

A simple model of photosynthesis is described which is dependenton leaf area, organic nitrogen content and distribution withinthe canopy as well as on the light and temperature environments.The model is parameterized using a cauliflower crop as an example.The optimized protein-N profile within the canopy is calculatedwith respect to daily growth rate. By comparison with measuredprotein-N contents, the amount of super-optimal N-uptake, i.e.the N-uptake which does not increase productivity, is assessedfor two different nitrogen and light treatments. The amountof super-optimal N accumulated in cauliflower depends on N-supplyand can exceed 80 kg N ha^-1. Copyright 2000 Annals of BotanyCompany Brassica oleracea L. botrytis, cauliflower, nitrogen, photosynthesis, respiration, model, optimization     

Effects of Physiological and Environmental Variations on Size-Structure Dynamics in Plant Populations

Sensitivity analysis was conducted, based on the canopy photosynthesisand continuity equation models which were developed in a previouspaper (Yokozawa and Hara, 1992), to investigate effects of variationin physiological parameters (maximal photosynthetic rate perunit leaf area, respiration rate per unit leaf area, maintenancerespiration rate per unit weight, growth respiration rate perunit weight, light extinction coefficient of the canopy, etc.)on the size-structure dynamics in plant populations. As thedegree of asymmetry in competition between individuals increased,effects of variation in physiological parameters diminished.Therefore, a population undergoing one-sided competition (mostasymmetric competition) is a stable system, little affectedby temporal and spatial variations in the environmental conditionswhich lead to variation in physiological parameters, whereasa population undergoing symmetric two-sided competition is sensitiveto these fluctuations. It was also shown by simulation thatthe degree of asymmetry in competition decreases (through effectson canopy photosynthesis) as nutrient level in the soil is reduced.It is suggested that symmetric two-sided competition is associatedwith non-transitivity of competition between species (i.e. frequentreversals of rank order of species), and hence with speciesdiversity. Several other ecological phenomena are discussedin relation to allometry (i.e. allocation-growth pattern) andthe degree of asymmetry in competition.Copyright 1994, 1999Academic Press Allometry, canopy photosynthesis, competition mode, continuity equation, parameter sensitivity, stability of stand structure     

Profiles of Leaf Nitrogen and Light in Reproductive Canopies of Cotton (Gossypium hirsutum)

During vegetative growth, the vertical profile of leaf nitrogen(N) often parallels the profile of light distribution withinthe canopy. This is more advantageous in terms of canopy photosynthesisthan a uniform distribution of leaf N. We investigated the influenceof both reproductive growth and N supply on the profiles ofN and light in canopies of irrigated cotton crops (Gossypiumhirsutum L.). Regular samplings were made from soon after theonset of reproductive growth until crop maturity. Every 2 weeks,a 1 m²sample of the canopy was cut in four successive verticallayers of equal thickness. Leaf area and N concentration (%)in each layer were measured. The vertical N gradient becamemore marked with ongoing reproductive development. It is hypothesizedthat because of the high rate of growth after the onset of reproductivedevelopment and the long duration of this phase compared toother species, the whole canopy photosynthetic benefit thatwould accrue from maintaining the N gradient is likely to beaccentuated. The rate of decline in leaf N concentration ina layer was not related to either the initial concentrationin the leaves nor the boll load within the layer.Copyright 2001Annals of Botany Company Gossypium hirsutum, leaf nitrogen, light profile, nitrogen, nitrogen distribution, remobilization, reproductive growth     

The phenology of leaf quality and its within‐canopy variation is essential for accurate modeling of photosynthesis in tropical evergreen forests

Leaf quantity (i.e., canopy leaf area index, LAI), quality (i.e., per‐area photosynthetic capacity), and longevity all influence the photosynthetic seasonality of tropical evergreen forests. However, these components of tropical leaf phenology are poorly represented in most terrestrial biosphere models (TBMs). Here, we explored alternative options for the representation of leaf phenology effects in TBMs that employ the Farquahar, von Caemmerer & Berry (FvCB) representation of CO₂ assimilation. We developed a two‐fraction leaf (sun and shade), two‐layer canopy (upper and lower) photosynthesis model to evaluate different modeling approaches and assessed three components of phenological variations (i.e., leaf quantity, quality, and within‐canopy variation in leaf longevity). Our model was driven by the prescribed seasonality of leaf quantity and quality derived from ground‐based measurements within an Amazonian evergreen forest. Modeled photosynthetic seasonality was not sensitive to leaf quantity, but was highly sensitive to leaf quality and its vertical distribution within the canopy, with markedly more sensitivity to upper canopy leaf quality. This is because light absorption in tropical canopies is near maximal for the entire year, implying that seasonal changes in LAI have little impact on total canopy light absorption; and because leaf quality has a greater effect on photosynthesis of sunlit leaves than light limited, shade leaves and sunlit foliage are more abundant in the upper canopy. Our two‐fraction leaf, two‐layer canopy model, which accounted for all three phenological components, was able to simulate photosynthetic seasonality, explaining ~90% of the average seasonal variation in eddy covariance‐derived CO₂ assimilation. This work identifies a parsimonious approach for representing tropical evergreen forest photosynthetic seasonality in TBMs that utilize the FvCB model of CO₂ assimilation and highlights the importance of incorporating more realistic phenological mechanisms in models that seek to improve the projection of future carbon dynamics in tropical evergreen forests.     

Growth of Individuals in Plant Populations

Relationships between individual plant weight and net photosynthesisper day (G(t, x) function of plant weight) in plant populationsof various stand structures were simulated based on a canopyphotosynthesis model. The G(t, x) functions of plant weightare determined mainly by LAI (leaf area index), the relationshipbetween individual plant weight and leaf area, canopy structureand extinction coefficient. The concave relationship betweenindividual plant weight and leaf area at small LAI (<2),at small extinction coefficient (< 0.5), or at the canopystructure having the maximum leaf area density at the bottomproduces a concave G(t, x) function, which generates negativeskewness of plant weight. The linear relationship between individualplant weight and leaf area at large LAI (> 2) produces aconvex G(t, x) function, which generates positive skewness ofplant weight. These simulation results coincide with G(t, x)functions obtained experimentally and with the well-known phenomenonof stand dynamics in which skewness of plant weight becomesnegative in the early growth stage and then increases to a positivevalue as a stand grows and becomes crowded. Helianthus annuus L., individual plant size, mean growth rate, canopy photosynthesis model, skewness, stand structure     

Morphological and phenological shoot plasticity in a Mediterranean evergreen oak facing long-term increased drought

Mediterranean trees must adjust their canopy leaf area to the unpredictable timing and severity of summer drought. The impact of increased drought on the canopy dynamics of the evergreen Quercus ilex was studied by measuring shoot growth, leaf production, litterfall, leafing phenology and leaf demography in a mature forest stand submitted to partial throughfall exclusion for 7 years. The leaf area index rapidly declined in the throughfall-exclusion plot and was 19% lower than in the control plot after 7 years of treatment. Consequently, leaf litterfall was significantly lower in the dry treatment. Such a decline in leaf area occurred through a change in branch allometry with a decreased number of ramifications produced and a reduction of the leaf area supported per unit sapwood area of the shoot (LA/SA). The leafing phenology was slightly delayed and the median leaf life span was slightly longer in the dry treatment. The canopy dynamics in both treatments were driven by water availability with a 1-year lag: leaf shedding and production were reduced following dry years; in contrast, leaf turnover was increased following wet years. The drought-induced decrease in leaf area, resulting from both plasticity in shoot development and slower leaf turnover, appeared to be a hydraulic adjustment to limit canopy transpiration and maintain leaf-specific hydraulic conductivity under drier conditions.     

The Seasonal Pattern of Growth and Production of a Temperate C4 Species, Cyperus longus

Growth and production of the temperate C₄ species Cyperus longusL. was measured throughout a growing season in an establishedplot in Eastern Ireland. The maximum standing live biomass reachedwas 2·5 kg m^–2. Estimates of unit leaf rate (ULR)and leaf area index (LAI) were made. The product of these quantitiesgave the crop growth rate (CGR) each week. C. longus was foundto maintain high values of LAI throughout the summer, with amaximum value of about 13 in early August. CGR reached a peakin early July. The optimum LAI was 11·6. Temperaturesat five levels in the plant canopy, and the amount of solarradiation intercepted by the canopy were measured continuouslyduring the summer. The mean daily rate of leaf extension waspositively correlated with the mean daily air temperature abovethe canopy but the temperature coefficient of the process waslow compared with other temperate species. The percentage ofsolar radiation intercepted by the canopy increased rapidlyin early summer, and canopy closure had occurred by mid-June.Rates of net photosynthesis were measured on young and old leafmaterial in situ at the time of peak LAI. In young leaves themaximum rates of net photosynthesis were higher than those publishedfor a range of temperate C₃ species, but similar to those foundin another temperate C₄ species, Spartina townsendii. Key words: C₄ photosynthesis, leaf growth, productivity     

A model of dynamics of leaves and nitrogen in a plant canopy: an integration of canopy photosynthesis,leaf life span,and nitrogen use efficiency

A model of dynamics of leaves and nitrogen is developed to predict the effect of environmental and ecophysiological factors on the structure and photosynthesis of a plant canopy. In the model, leaf area in the canopy increases by the production of new leaves, which is proportional to the canopy photosynthetic rate, with canopy nitrogen increasing with uptake of nitrogen from soil. Then the optimal leaf area index (LAI; leaf area per ground area) that maximizes canopy photosynthesis is calculated. If leaf area is produced in excess, old leaves are eliminated with their nitrogen as dead leaves. Consequently, a new canopy having an optimal LAI and an optimal amount of nitrogen is obtained. Repeating these processes gives canopy growth. The model provides predictions of optimal LAI, canopy photosynthetic rates, leaf life span, nitrogen use efficiency, and also the responses of these factors to changes in nitrogen and light availability. Canopies are predicted to have a larger LAI and a higher canopy photosynthetic rate at a steady state under higher nutrient and/or light availabilities. Effects of species characteristics, such as photosynthetic nitrogen use efficiency and leaf mass per area, are also evaluated. The model predicts many empirically observed patterns for ecophysiological traits across species.     

Weak relationships between leaf phenology and isohydric and anisohydric behavior in lowland wet tropical forest trees

Wet tropical forest trees display a wide range of leaf phenology dynamics. However, the interrelation between deciduousness, water status, and leaf and stem characteristics have been poorly investigated compared with dry forests. We studied wet forest trees to answer the following questions: (1) do water regulation modes (iso/anisohydric behavior) of evergreen species differ from those found in deciduous species? (2) Does leaf water potential (Ψ_L) influences leaffall and emergence dynamics? (3) Are leaf and stem characteristics consistent across evergreen and deciduous trees? We evaluated vegetative phenology, Ψ_L, and leaf and stem characteristics of six evergreen and three deciduous species monthly for 2 yr. Species exhibited different leaffall and emergence dynamics, as well as different water regulation modes, independent of their deciduousness. Thus, the relationship between leaf phenology and water regulation behaviors appears to be a species‐specific property rather than a functional group attribute. Ψ_L had no direct influence on the dynamics of leaffall and/or emergence, indicating that this process is not modulated by water availability alone. Individual groups of evergreen and deciduous species could not be identified using principal component analysis (PCA), but a decoupling was observed in the leaf and stem economics spectra. The lack of an interrelation between deciduousness and iso/anisohydry, as well as the independence of leaf and stem trade‐offs, emphasizes that more systematic measurements of vegetative phenology and ecophysiological characteristics are necessary to advance our knowledge of leaf habit and water regulation behaviors based on the functional traits of wet forest plants.     

An Analysis of Spatial Variation in the Nitrogen Content of Leaves from Different Horizons Within a Canopy

An analysis of spatial variation in the specific nitrogen contentof leaves from different positions within a canopy is developed.It is used to examine data obtained for contrasting crop species;the legume crop guar (Cyamopsis tetragonoloba), and crops ofa forage sorghum. The analysis distinguishes two componentsof the leaf nitrogen content: one associated with the metabolicapparatus within the leaf tissues and the other with structuralelements of the leaf. The analysis allows the spatial variationin specific leaf nitrogen content to be quantitatively described. Leaf nitrogen content, crop growth, light interception     

The Relationship Between Size of Shoot Apices and Morphological Features of Mature Leaves and Stems of Four Species of Angiosperm Trees

Shoot apex, leaf and stem growth parameters for four speciesof deciduous trees were measured. Only in elm was there a correlationbetween the size of shoot apical meristems and mature leaves.In ash, basswood and cottonwood there was no significant differencebetween size of shoot apices of sucker and canopy branches,despite significant differences in lamina size. In the suckerbranches of all species studied there occurred an early, lateralexpansion of the subapical region of the shoot apical meristem.This correlated well with the greater diameter of stem and pithregions of sucker branches. In addition, the season's annualring of xylem was greater in basswood, cottonwood and elm. Diametersof vessel elements were greater in sucker than canopy branchesin three of the four species. Total branch and internode andnumber of nodes per branch were significantly greater for suckerbranches than canopy growth of all species studied. A hypothesis is proposed to explain the development of the verylarge surface area of leaves on sucker branches. This hypothesisis based on the position of sucker branches in relation to theroot system and involves differences in water stress known tobe present in all plants.Copyright 1993, 1999 Academic Press Sucker leaves, canopy leaves, Fraxìnus pennsylvanica Marsh, green ash, Ulmus amerìcana L., American elm, Populus deltoides Marsh, cottonwood, Tilia americana, basswood     

Impact of tree leaf phenology on growth rates and reproduction in the spring flowering species Trillium erectum (Liliaceae)

We investigated the impact of overstory tree leaf phenology on growth rates, carbon allocation pattern, and fruit characteristics in the spring flowering species, Trillium erectum (Liliaceae). Air temperature, overstory canopy closure, and T. erectum phenology were monitored at three locations following a latitudinal gradient in Québec, Canada. Northern sugar maple trees leaf out at cooler temperatures than more southern populations, while Trillium development was initiated at the same soil temperature irrespective of the latitude. Therefore, in northern areas, the time between initiation of T. erectum leaf expansion and canopy closure was shorter than in southern areas, which left less time for northern plants to accumulate reserves before canopy closure. Differences in growth patterns were noted between T. erectum populations. From a south-north gradient, investment to reproduction, total plant biomass, and annual growth rate decreased, while specific leaf area and stem height increased, indicating shade acclimation. The length of the high light period in early spring seems to be a determinant for spring flowering plants' growth and reproduction and may explain the northern distribution limit of some of these species.     

Seasonality and phenology alter functional leaf traits

In plant ecophysiology, functional leaf traits are generally not assessed in relation to phenological phase of the canopy. Leaf traits measured in deciduous perennial species are known to vary between spring and summer seasons, but there is a knowledge gap relating to the late-summer phase marked by growth cessation and bud set occurring well before fall leaf senescence. The effects of phenology on canopy physiology were tested using a common garden of over 2,000 black cottonwood (Populus trichocarpa) individuals originating from a wide geographical range (44–60ºN). Annual phenological events and 12 leaf-based functional trait measurements were collected spanning the entire summer season prior to, and following, bud set. Patterns of seasonal trait change emerged by synchronizing trees using their date of bud set. In particular, photosynthetic, mass, and N-based traits increased substantially following bud set. Most traits were significantly different between pre-bud set and post-bud set phase trees, with many traits showing at least 25 % alteration in mean value. Post-bud set, both the significance and direction of trait–trait relationships could be modified, with many relating directly to changes in leaf mass. In Populus, these dynamics in leaf traits throughout the summer season reflected a shift in whole plant physiology, but occurred long before the onset of leaf senescence. The marked shifts in measured trait values following bud set underscores the necessity to include phenology in trait-based ecological studies or large-scale phenotyping efforts, both at the local level and larger geographical scale.     

Modelling canopy CO2 fluxes: are ‘big‐leaf’ simplifications justified?

• 1 The ‘big‐leaf’ approach to calculating the carbon balance of plant canopies is evaluated for inclusion in the ETEMA model framework. This approach assumes that canopy carbon fluxes have the same relative responses to the environment as any single leaf, and that the scaling from leaf to canopy is therefore linear.
• 2 A series of model simulations was performed with two models of leaf photosynthesis, three distributions of canopy nitrogen, and two levels of canopy radiation detail. Leaf‐ and canopy‐level responses to light and nitrogen, both as instantaneous rates and daily integrals, are presented.
• 3 Observed leaf nitrogen contents of unshaded leaves are over 40% lower than the big‐leaf approach requires. Scaling from these leaves to the canopy using the big‐leaf approach may underestimate canopy photosynthesis by ~20%. A leaf photosynthesis model that treats within‐leaf light extinction displays characteristics that contradict the big‐leaf theory. Observed distributions of canopy nitrogen are closer to those required to optimize this model than the homogeneous model used in the big‐leaf approach.
• 4 It is theoretically consistent to use the big‐leaf approach with the homogeneous photosynthesis model to estimate canopy carbon fluxes if canopy nitrogen and leaf area are known and if the distribution of nitrogen is assumed optimal. However, real nitrogen profiles are not optimal for this photosynthesis model, and caution is necessary in using the big‐leaf approach to scale satellite estimates of leaf physiology to canopies. Accurate prediction of canopy carbon fluxes requires canopy nitrogen, leaf area, declining nitrogen with canopy depth, the heterogeneous model of leaf photosynthesis and the separation of sunlit and shaded leaves. The exact nitrogen profile is not critical, but realistic distributions can be predicted using a simple model of canopy nitrogen allocation.

     

Predicting the Growth Interactions between Plants in Mixed Species Stands using a Simple Mechanistic Model

The Conductance model is a simple mechanistic model used topredict the growth of species in monoculture or mixtures fromparameter values derived from plants grown in isolation. Incontrast to many mechanistic models that require extensive parameterization,the Conductance model is able to capture the growth of a broadrange of species using a few simplified assumptions regardingplant growth and easily derived species-specific parameter values.We examine the assumptions within the Conductance model thattotal leaf area per plant is proportional to total plant weight,and that an isolated plant has a projected crown zone area thatis proportional to the 2/3 power of its weight. Power ratherthan linear relations were found between weight and leaf areafor Brassica oleracea, Daucus carota, Matricaria inodora, Solanumnigrum,Stellaria media , Trifolium repens and Veronica persica.For all seven species, the value of the power was less thanunity. All species also exhibited a power relation between crownzone area and weight, with the slope of this relation beingless than 2/3 for B. oleracea, D. carota and S. media. Althoughmorphology type accounted for some of the variation in the parametervalues relating to light interception, there were considerabledifferences between species within upright or prostrate foliagespecies groups. The Conductance model was used to predict yieldsof B. oleracea, S. nigrum and V. persica grown in both monocultureand binary weed-crop mixtures over a range of temporal and spatialscales. After calibrating the model to non-competing plants,the model was used to predict growth of the weed and crop speciesin contrasting densities and stand types. In some crop-weedcombinations, predicted crop and weed weights were within 17%of observed values, with no systematic deviations. In others,systematic and large deviations occurred.Copyright 2001 Annalsof Botany Company Brassica oleracea L., Daucus carota L., Matricaria inodora L., Solanum nigrum L.,Stellaria media L., Trifolium repens L., Veronica persica L., competition, growth, leaf area, crown zone area, light, shoot morphology, canopy architecture     

New insights on plant phenological response to temperature revealed from long‐term widespread observations in China

Constraints of temperature on spring plant phenology are closely related to plant growth, vegetation dynamics, and ecosystem carbon cycle. However, the effects of temperature on leaf onset, especially for winter chilling, are still not well understood. Using long‐term, widespread in situ phenology observations collected over China for multiple plant species, this study analyzes the quantitative response of leaf onset to temperature, and compares empirical findings with existing theories and modeling approaches, as implemented in 18 phenology algorithms. Results show that the growing degree days (GDD) required for leaf onset vary distinctly among plant species and geographical locations as well as at organizational levels (species and community), pointing to diverse adaptation strategies. Chilling durations (CHD) needed for releasing bud dormancy decline monotonously from cold to warm areas with very limited interspecies variations. Results also reveal that winter chilling is a crucial component of phenology models, and its effect is better captured with an index that accounts for the inhomogeneous effectiveness of low temperature to chilling rate than with the conventional CHD index. The impact of spring warming on leaf onset is nonlinear, better represented by a logistical function of temperature than by the linear function currently implemented in biosphere models. The optimized base temperatures for thermal accumulation and the optimal chilling temperatures are species‐dependent and average at 6.9 and 0.2°C, respectively. Overall, plants’ chilling requirement is not a constant, and more chilling generally results in less requirement of thermal accumulation for leaf onset. Our results clearly demonstrate multiple deficiencies of the parameters (e.g., base temperature) and algorithms (e.g., method for calculating GDD) in conventional phenology models to represent leaf onset. Therefore, this study not only advances our mechanistic and quantitative understanding of temperature controls on leaf onset but also provides critical information for improving existing phenology models.     

Seasonal patterns of leaf production in co‐occurring trees with contrasting leaf phenology: time and quantitative divergences

Neotropical savannas (‘cerrados’) of Central Brazil are characterized by the coexistence of a large diversity of tree species with divergent phenological behaviors, which reflect a great diversity in growth strategies. In the present study time behavior and quantitative aspects of shoot growth, shoot mortality, and leaf longevity and production were analyzed in 12 woody species of contrasting leaf phenology, adopting a functional group approach where 12 species were categorized into three functional groups: evergreen, decidous and brevideciduous, according to their leaf phenology. Shoot growth and leaf production were seasonal for the three functional groups, differing in their time of occurrence, but being concentrated during the last months of the dry season. Shoot growth differed between evergreens and deciduous, as well leaf production. Evergreens had higher rates of shoot growth, produced a higher number of leaves and had longer leaf longevity (around 500 days against 300 days in deciduous and brevideciduous). Leaf longevity was associated with patterns of leaf production when accounting for all phenological groups studied. It was possible to identify different patterns of aerial growth in savanna phenological groups, providing evidence of great functional variability amongst the groups studied.     

胡杨枝芽生长特征及其展叶物候特征

以5个不同发育阶段的胡杨(Populus euphratica Oliv.)个体为研究对象,观测记录了枝芽展叶物候、枝芽生长特征和叶形变化的空间分布规律。结果表明:不同发育阶段的胡杨个体以及同一个体树冠的不同层次,其枝芽生长及其展叶物候期表现出不同的时空特征。随着树龄的增加和树冠层次的增高(由基向顶),当年新生枝条长度、枝条叶片数和叶形指数逐渐减小,但叶面积和叶片干重逐渐增大。5个不同发育阶段胡杨个体均表现出展叶物候始于树冠顶层,依次向下结束于树冠基部;展叶物候期共性表现在枝芽萌动期均在4月上旬,起始展叶期集中在4月中旬,展叶终期则在5月上旬到下旬;树龄较大的个体其枝芽萌动期、起始展叶期、展叶终期较树龄较小的个体早;其枝芽萌动期到展叶终期的时间进程较树龄较小的个体短;不同发育阶段的个体枝芽萌动期出现的时间较为离散,起始展叶期和展叶终期出现的时间较为集中。相关分析表明,出叶周期与枝条长度、枝条叶片数量和叶形指数呈极显著正相关,与叶面积和叶片干重呈显著负相关。     

棉花冠层高光谱参数与叶片氮含量的定量关系

建立棉花(Gossypium hirsutum)氮素状况的光谱监测技术对于棉花营养诊断和长势估测具有重要意义。该研究利用冠层高光谱反射率及演变的多种高光谱参数,分析了不同施氮水平下不同棉花品种叶片氮含量与冠层反射光谱的定量关系,建立了棉花叶片氮含量的敏感光谱参数及预测方程。结果显示,棉花叶片氮含量和冠层高光谱反射率随不同施氮水平呈显著变化。棉花叶片氮含量的敏感光谱波段为600~700 nm的可见光波段和750~900 nm的近红外波段,且叶片氮含量与比值植被指数RVI [average (760~850), 700]有密切的定量关系,4个品种的平均决定系数在0.70左右。进一步分析表明,可以用统一的回归方程来描述不同品种、不同生育时期和不同氮素水平下棉花叶片氮含量随反射光谱参数的变化模式,从而为棉花氮素营养的监测诊断与精确施肥提供技术依据。