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青蒿毛状根合成青蒿素的培养条件研究 总被引:14,自引:0,他引:14
对影响青蒿(ArtemisiaannuaL.)毛状根生长及青蒿素合成的培养条件进行了研究,确定最适的培养条件为:初始pH5.8~6.0,摇瓶转速130~150r/min,摇瓶装液量体积分数为25%,光照周期为16h/d,温度为30℃。在此条件下,经过25d培养获得青蒿素产量为223.3mg/L。 相似文献
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青蒿素在露水草毛状根中的生物转化 总被引:11,自引:0,他引:11
露水草毛状根培养系中加入青蒿素培养8d后,青蒿素转化去氧青蒿素。根据光谱数据,对去氧青蒿素的结构进行了鉴定。结果表明,通过水草毛状根能将青蒿素进行选择性还原为去氧青蒿素。 相似文献
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利用自制的气升式内环流生物反应器进行青蒿(Artemisia
annua L.)毛状根多层培养生产青蒿素。毛状根培养物在培养过程中均匀分布在生物反应器的筛网间,或以不锈钢网为附着点向四周生长,在25
℃和12 h/d光照周期下,经20 d分批培养获得生物量干重22.57 g/L,青蒿素产量374.4
mg/L,并对培养过程中蔗糖、磷酸盐、硝酸盐和氨盐消耗的动力学进行了分析。 相似文献
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利用气升式内环流生物反应器培养青蒿毛状根生产青蒿素 总被引:8,自引:0,他引:8
利用自制的气升式内环流生物反应器进行青蒿(ArtemisiaannuaL.)毛状根多层培养生产青蒿素。毛状根培养物在培养过程中均匀分布在生物反应器的筛网间,或以不锈钢网为附着点向四周生长,在25℃和12h/d光照周期下,经20d分批培养获得生物量干重22.57g/L,青蒿素产量374.4mg/L,并对培养过程中蔗糖、磷酸盐、硝酸盐和氨盐消耗的动力学进行了分析。 相似文献
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栝楼毛状根的生长与营养消耗动态研究 总被引:3,自引:0,他引:3
研究了栝楼毛状根在摇瓶培养条件下的生长与营养消耗规律。栝楼毛状根的生长过程可以分为四个生长阶段。生长迟滞期,缓慢生长期,快速生长期和衰亡期。在迟滞期,培养液中的蔗糖,氮,磷,钾首先被快速吸收,然后又有所回升;在缓慢生长期,培养液中的葡萄糖,果糖,氮,磷和钾的浓度开始降低,到了快速生长期,培养液中的碳源,氮,磷,钾等营养物质被快速消耗,到了衰亡期,以上的营养物质基本被消耗殆尽,Ca^2 从接种开始缓慢下降,当毛状根进入衰亡期以后,由于老化细胞的损伤而有部分钙离子外泻,栝楼毛状根的蛋白质也开始快速分泌。 相似文献
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在植物组织和细胞培养过程中,尤其是在生物反应器培养中的染菌问题,一直是制约植物细胞培养工业化的难题,通过比较各种防腐剂的抑菌效果。确定银型磷酸锆盐抗菌粉为青蒿根培养的最佳防腐剂,银型磷酸锆盐抗菌粉在浓度为30mg/L时,既能降低培养液的染菌几率,又不明显抑制青蒿根的生长及青蒿素的生物合成,在添加30mg/L抗菌粉的培养液中进行的青蒿根生长,pH值变化以及残糖,铵离子和硝酸根离子消耗的动力学研究表明,在40d内青蒿根在培养液中生长良好,营养成分的消耗和对照呈相似的趋势。 相似文献
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在植物组织和细胞培养过程中,尤其是在生物反应器培养中的染菌问题,一直是制约植物细胞培养工业化的难题.通过比较各种防腐剂的抑菌效果,确定银型磷酸锆盐抗菌粉为青蒿根培养的最佳防腐剂.银型磷酸锆盐抗菌粉在浓度为30 mg/L时,既能降低培养液的染菌几率,又不明显抑制青蒿根的生长及青蒿素的生物合成.在添加30 mg/L抗菌粉的培养液中进行的青蒿根生长、pH值变化以及残糖、铵离子和硝酸根离子消耗的动力学研究表明,在40 d内青蒿根在培养液中生长良好,营养成分的消耗和对照呈相似的趋势. 相似文献
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添加GA3和CCC虽然影响栝楼毛状根的生物量积累 ,但是有利于促进天花粉蛋白 (TCN)的合成 ,当GA3的添加量为 2mg/L时 ,天花粉蛋白含量增加了 18 9% ,添加 1mg/L~ 2mg/LCCC的天花粉蛋白提高了 2 8%。分别添加维生素B1或B5会延长毛状根的生长迟滞期 ,但是 ,后期的生长非常迅速 ,并可以促进天花粉蛋白的合成。同时添加维生素B1和B5可以缩短生长迟滞期 ,并能促进毛状根的生长 ,同时添加1mg/LB1 4mg/LB5有利于促进天花粉蛋白总量的增加。 相似文献
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从747条发根农杆菌ATCC15834转化的青蒿株系025发根中,筛选出7个生长较快的发根系,这7个系在生长速度和青蒿素含量上均有显著差异,其中发根系HR9青蒿素产率最高,达到每月3325mg/L。青蒿发根的生长量和青蒿素含量极显著高于未转化根和愈伤组织。青蒿发根在分批培养中没有明显的迟滞期,接种后第7天进入指数生长期,第11天生长最快,第20天进入稳定期。青蒿发根中青蒿素含量呈明显的“与生长相关”特性,在指数生长期,青蒿素含量缓慢下降,生长速度减缓后,青蒿素含量上升,发根生长停止后,继续延长培养时间,青蒿素含量也不再提高。在分批培养中,青蒿发根适宜的培养时间为21d。 相似文献
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真菌诱导子对青蒿发根细胞生长和青蒿素积累的影响 总被引:8,自引:0,他引:8
3种真菌诱导子(大菌丽花轮枝孢(Verticillium dahiae Kleb.)、葡枝根霉(Rhizopus stolonifer(Ehrenb.exFr.)Vuill)和束状刺盘孢(Colletorichum dematium(Pers.)Grove)处理青蒿(Artemisia annuaL.)的发根,均能促进发根中青蒿素的积累,其中以大丽花轮枝孢的诱导效果最好;对细胞生长均没有明显影响, 相似文献
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WANG Hong 《植物学报(英文版)》2000,42(9):905-909
The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr. ) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae, the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment. 相似文献
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《Journal of Plant Interactions》2013,8(1):811-824
Artemisinin is frequently used in the artemisinin-based combination therapy to cure drug-resistant malaria in Asian subcontinent and large swath of Africa. The hairy root system, using the Agrobacterium rhizogenes LBA 9402 strain to enhance the production of artemisinin in Artemisia annua L., is developed in our laboratory. The transgenic nature of hairy root lines and the copy number of transgene (rol B) were confirmed using polymerase chain reaction and Southern Blot analyses, respectively. The effect of different concentrations of methyl jasmonate (MeJA), fungal elicitors (Alternaria alternate, Curvularia limata, Fusarium solani, and Piriformospora indica), farnesyl pyrophosphate, and miconazole on artemisinin production in hairy root cultures were evaluated. Among all the factors used individually for their effect on artemisinin production in hairy root culture system, the maximum enhancement was achieved with P. indica (1.97 times). Increment of 2.44 times in artemisinin concentration by this system was, however, obtained by combined addition of MeJA and cell homogenate of P. indica in the culture medium. The effects of these factors on artemisinin production were positively correlated with regulatory genes of MVA, MEP, and artemisinin biosynthetic pathways, viz. hmgr, ads, cyp71av1, aldh1, dxs, dxr, and dbr2 in hairy root cultures of A. annua L. 相似文献
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State of the art of the production of the antimalarial compound artemisinin in plants 总被引:6,自引:0,他引:6
Geldre Els Van Vergauwe Annemieke Eeckhout Elfride Van den 《Plant molecular biology》1997,33(2):199-209
For more than three centuries we have relied on the extracts of the bark of Cinchona species to treat malaria. Now, it seems we may be changing to the leaves of a Chinese weed, Artemisia annua, and its active compound artemisinin. Artemisinin-derived drugs have been proved particularly effective treatments for severe malaria, even for multi-drug-resistant malaria. However, this promising antimalarial compound remains expensive and is hardly available on a global scale. Therefore, many research groups have directed their investigations toward the enhancement of artemisinin production in A. annua cell cultures or whole plants in order to overproduce artemisinin or one of its precursors. This article provides a brief review of the state of art of the different aspects in A. annua research. 相似文献
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探讨了光照、温度和培养方式对青蒿芽生长和青蒿素合成的影响。适宜芽生长和青蒿素积累的光照强度约为3 000 lx,照光时间为20 h/d ;芽生长和青蒿素积累的最适温度分别为25 ℃和30 ℃,通过先25 ℃(25 d)后30 ℃(5 d) 的温度转变二步培养法可以提高青蒿素的产量;青蒿芽生长和青蒿素积累的最佳培养方式为非浸没低转速摇瓶培养。 相似文献
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Improved growth of Artemisia annua L hairy roots and artemisinin production under red light conditions 总被引:6,自引:0,他引:6
Hairy root cultures of Artemisia annua L were cultivated for 30 days under either white, red, blue, yellow or green light. Red light at 660 nm gave the highest biomass of hairy roots (5.73 g dry wt cells l–1 medium) and artemisinin content (31 mg arteminsinin g–1 dry cells) which were, respectively, 17% and 67% higher than those obtained under white light. 相似文献