THE EFFECT OF SEAWEED DIETS ON GROWTH OF GREEN ABALONE,HALIOTIS FULGENS,FROM BAJA CALIFORNIA,MEXICO

Two abalone species: green Haliotis fulgens and yellow Halioti corrugata represent nearly 97% of the total production in the Mexican abalone fishery. It has been assumed that abalone feed on the kelp algae Macrocystis pyrifera. Regional hatcheries use this species as a main source of natural food. M. pyrifera does not occur at the southern limit of the distribution of abalone species along the Baja California Peninsula. In this study, growth rates of juveniles H. fulgens, 17.3 ± 2.2 mm shell length and 0.4 ± 0.2 g body weight, were evaluated. Juveniles were fed with common species in the benthic environments inhabited by abalone along the western coast of Baja California during 191 days. Three diets were based on algae: palm kelp, Eisenia arborea, giant kelp, M. pyrifera and Gelidium robustum, and one on seagrass, Phyllospadix torreyi. Shell length and body growth rates varied between 21.5 μm day^?1 and 2.2 mg day^?1 for E. arborea and between 45.9 μm day^?1 and 6.7 mg day^?1 for M. pyrifera. Higher specific growth rates (SGR) in length and weight were determined for M. pyrifera: 0.2% and 0.7% day^?1. Significant differences between values of juveniles fed M. pyrifera with the rest of the diets were found. The highest mortality (21%) was in juveniles fed the red algae G. robustum.     

Food intake and growth in the blue-spotted trevally, Caranx bucculentus Alleyne and Macleay 1877, with reference to predation on penaeid prawns

Blue-spotted trevally, Caranx bucculentus , were fed different rations of pilchard and prawn in order to investigate feeding and growth relationships. Maintenance rations at 25.5° C amounted to 3.7% B.W. day⁻¹ and 2.7% B.W. day⁻¹ for prawns and pilchards, respectively. Additional feeding experiments at 28.9° C yielded a maintenance ration of prawns of 3.8% B.W. day⁻¹, suggesting there is very little if any temperature effect on the feeding-growth relationship over the range studied. Fish fed twice or more each day consumed about 7.3 ± 1.4% B.W. day⁻¹.
Given the biomass of this trevally in Albatross Bay, Gulf of Carpentaria, and the contribution of prawns to its diet, we estimate consumption of commercial prawns at 25 ± 5 g.ha⁻¹ day⁻¹ or 11 g kg⁻¹ day⁻¹.     

Annual changes of bacterial mortality due to viruses and protists in an oligotrophic coastal environment (NW Mediterranean)

The impact of viruses and protists on bacterioplankton mortality was examined monthly during 2 years (May 2005–April 2007) in an oligotrophic coastal environment (NW Mediterranean Sea). We expected that in such type of system, (i) bacterial losses would be caused mainly by protists, and (ii) lysogeny would be an important type of virus–host interaction. During the study period, viruses and grazers together were responsible for 50.6 ± 40.1% day⁻¹ of bacterial standing stock losses (BSS) and 59.7 ± 44.0% day⁻¹ of bacterial production losses (BP). Over the first year (May 2005–April 2006), protists were the principal cause of bacterial mortality, removing 29.9 ± 20.4% day⁻¹ of BSS and 33.9 ± 24.3% day⁻¹ of BP, whereas viral lysis removed 13.5 ± 17.0% day⁻¹ of BSS and 12.3 ± 12.3% day⁻¹ of BP. During the second year (May 2006–April 2007), viruses caused comparable bacterial losses (29.2 ± 14.8% day⁻¹ of BSS and 40.9 ± 20.7% day⁻¹ of BP) to protists (28.6 ± 25.5% day⁻¹ of BSS and 32.4 ± 20.0% day⁻¹ of BP). In 37% of cases higher losses of BP due to viruses than due to protists were found. Lysogenic infection was detected in 11 of 24 samplings. Contrary to our expectations, lytic infections dominated over the two years, and viruses resulted to be a significant source of bacterial mortality in this oligotrophic site.     

Population growth rates of two closely related rotifer species: effects of food quantity, particle size, and nutritional quality

SUMMARY. 1. The relationship between population growth rates and the concentrations of several algal species was determined in laboratory experiments with the rotifers Brachionus rubens and B. calyciflorus .
2. The effects of food quantity were well described by a modified Monod model with a threshold for zero population growth. The model parameters depended on particle size and nutritional quality of the food algae. Differences between the rotifer species were significant and reflected their varying food-size preferences.
3. For each rotifer species, thresholds were lowest for algae in the most readily ingested size range. The lowest thresholds were 0.07–0.09 mgC 1⁻¹ with algae of about 5 μm equivalent spherical diameter (ESD) for B. rubens , and 0.19 mgC 1⁻¹ with algae of about 10 μm ESD for B. calyciflorus .
4. Maximal growth rates ( r _max) were slightly below 0.8 day⁻¹ for both rotifers with most algal species. The highest r _max values for both rotifers were observed when Cyclotella meneghiniana was provided as food. With this alga, B. calyciflorus had a significantly higher r_max (1.02 day⁻¹) than B. rubens (0.838 day⁻¹).
5. From a comparison of the relationship between growth rates and ingestion rates, Chlamydomonas reinhardii appeared to be of low nutritional quality for B. rubens .
6. Egg ratios were related to growth rate and were not influenced by the algal food used. Egg development times and average mortality rates were estimated from the relationship between egg ratio and growth rate. B. calyciflorus appeared to have a high average mortality rate (0.383 day⁻¹) compared to B. rubens (0.083 day⁻¹).     

Feeding and growth of early juvenile Japanese sardines in the Pacific waters off central Japan

Larval and early juvenile growth was backcalculated for individual Japanese sardines Sardinops melanostictus using the biological intercept method based on the allometric relationship between otolith radii and fish lengths. Sardines grew at 0·81 mm day⁻¹ during the larval stage. In the early juvenile stage, they grew from 32·3 to 45·4 mm fork length ( L ) over a 20-day period (0·64mm day⁻¹). Using the observed relationship between L and wet body weight ( W ), W = 0·00942 L ^2.99, W of the sardine juveniles was calculated to increase from 306 to 832 mg during the 20-day period. The carbon (C) requirement to achieve this growth in weight was estimated to increase from 5·7 to 9·6 mg day⁻¹. Stomach contents of the sardines were composed mostly of copepods (73%) and larvaceans (25%). Wet stomach content weight ( W_s ) was expressed by a power function of the W , W_s=0·731 W ^0·658. Carbon and nitrogen constituted 41·7 ± 1·5 and 10·0 ± 0·4% of the dry W_s , respectively. Stomach C content increased from 2·0 to 3·9 mg during the 20-day period. Three to four cycles of the daily turnover of stomach contents during the 16 h of daytime, corresponding to a gastric evacuation rate of 0·2–0·3 h⁻¹ under continuous feeding, met the C requirement to achieve the backcalculated growth in early juvenile sardines. The Kuroshio frontal waters seem to provide Japanese sardine juveniles with favourable growth conditions.     

Productivity of a herbivorous pupfish population (Cyprinodon nevadensis) in a warm desert stream

Production and food intake by an herbivorous pupfish population ( Cyprinodon nevadensis amargosae ) living in the outflow of a thermal artesian well (Tecopa Bore) near Death Valley, California is described. Water issues from the ground at 47.5° C and cools 8.12° C before leaving the study area 300 m from the source. High stream temperatures restricted the pupfish population to some 41 % of the study area, with a resulting mean density of 89 individuals m⁻² (range = 13 to 196m⁻²). Biomass estimates ranged from 7 kcal m⁻² to 42 kcal m⁻². The mean annual standing crop of pupfishes (24 kcal m⁻²) turned over about five times annually. Growth rates were highest in juveniles (♂= 9.5% day⁻¹) and slowest in large adults (♀= 08% day⁻¹). Monthly production ranged from 22 kcal m⁻² in September to 3 kcal m⁻² in July and August. Pupfish in Tecopa Bore fed on algae and detritus, ingesting 1941 kcal m⁻² yr⁻¹ or 17.5% of the annual net primary production. 119 kcal m⁻² yr⁻¹ was deposited in growth. This latter value is approximately ten times greater than values previously reported for large carnivorous fishes but is comparable lo values reported for herbivorous fishes under pond culture.     

Seasonal variability in growth of larval Japanese anchovy Engraulis japonicus driven by fluctuations in sea temperature in the Kii Channel, Japan

Seasonal variability in the growth of larval Japanese anchovy Engraulis japonicus was examined through otolith microstructure analysis based on the samples collected from the northern side (inner area, IA) and the southern side (outer area, OA) of the Kii Channel from April 2006 to March 2007. Growth trajectories (otolith backcalculated mean standard length of 5 day intervals from 5 days after hatch to 24 days) as well as the most recent 5 day mean growth rate of larvae before capture ( G ₅) differed among months. Growth trajectories showed the same pattern as G ₅. In IA, mean ± s.d. G ₅ ranged from 0·31 ± 0·04 mm day⁻¹ (January) to 0·73 ± 0·06 mm day⁻¹ (October). In OA, mean ± s.d. G ₅ ranged from 0·36 ± 0·05 mm day⁻¹ (January) to 0·79 ± 0·11 mm day⁻¹ (August). G ₅ values declined from November to January and then started to increase. In general, the seasonal patterns of growth were similar between IA and OA, and a clear seasonal pattern in growth was identified. When the relationships among larval growth rate, sea temperature, zooplankton density and larval density were examined, growth rate was positively related with sea temperature in both areas and not related with the other factors. The similar pattern in growth observed between IA and OA was probably due to the low spatial variability in sea temperature compared to its seasonal variability.     

Protein synthesis, nitrogen excretion and long-term growth of juvenile Pleuronectes flesus

This study aimed to measure protein synthesis using a stable isotope method, investigate protein-nitrogen flux in a flatfish Pleuronectes flesus , and use the data to test the hypothesis that individual differences in growth efficiency were related to individual differences in protein-nitrogen flux mediated through differences in protein synthesis and degradation. Three measurements of protein-nitrogen flux via consumption, protein synthesis and nitrogenous excretion were made for individual flounder during a 212-day period and fractional rates of protein-nitrogen flux were scaled for a 50–g flounder to provide mean values for protein consumption (2·11 ± 0·21% day⁻¹), protein synthesis (2·08±0·23% day⁻¹), protein growth (0·71±0·06% day⁻¹) and protein degradation (1·37±0·24% day⁻¹). Mean rates of nitrogenous excretion were 0·142 mg N g⁻¹ day⁻¹ and 0·047 mg N g⁻¹ day⁻¹ for ammonia and urea, respectively. Individual flounder had different protein growth efficiencies and this was correlated negatively and significantly with mean rates of protein synthesis ( r - 0·70; P <0·05) and degradation ( r - 0·67; P < 0·05) and correlated positively and significantly with the efficiency of retaining synthesized protein ( r +0·63, P <0·05). This supported the proposed hypothesis that flounder which grow more efficiently achieve this through adopting a low protein turnover strategy.     

Effect of ration size on growth and gross conversion efficiency of young lemon sharks, Negaprion brevirostris

Young lemon sharks, Negaprion brevirostris , were kept under controlled conditions in an aquarium and fed blue runner, Caranx crysos , at different ration levels. The relationship between feeding rate and growth rate was best described by a von Bertalanffy growth curve, which predicted a maximum growth rate of 140 kJ kg⁻¹ day⁻¹ (0·66% b.w. day⁻¹), a maintenance ration of 199 kJ kg⁻¹ day⁻¹ (1·06% b.w. day⁻¹), and losses due to starvation of -236kJ kg⁻¹ day⁻¹ (1·11% b.w. day⁻¹). The relationship between gross conversion efficiency ( K ₁) and feeding rate was also examined. K₁ ranged from - 64 to 25% and did not drop at high ration levels. Activity levels of both starved sharks and sharks fed at maintenance were not significantly different (0·2 body lengths s⁻¹). K ₁ values generated from both laboratory and field data suggest that young lemon sharks can convert food to new tissue as efficiently as teleosts.     

Consumption, growth and respiration of bleak, Alburnus alburnus (L.), and roach, Rutilus rutilus (L.), during early ontogeny

Three components of the energy budget, consumption ( C ), production ( P ) and respiration ( R ) in juvenile roach and bleak kept under controlled food ( Anemia salina , 2400 ind. l⁻¹) and temperature (20° C) conditions were measured in a study aimed at defining differences between the two species and elucidating the patterns of energy partitioning during ontogeny.
Daily food consumption rates (J day⁻¹ fish⁻¹) increased allometrically ( C =a W ^b) with body size ( W , mg dry weight) in both species. Covariance analysis indicated no differences in slope or intercept for the two regression lines ( P ≤ 0.05, n = 82). However, the two species grew at significantly different rates, roach faster than bleak.
The dependence of the respiration rate (μmol h⁻¹ fish ⁻¹) on body weight ( W ) can be described by an allometric function: R = a W ^b, where a ± 95% C.L. = 0.17 ± 0.15 for roach and 0.18 ± 0.20 for bleak. The slope for roach (b ± 95% C.L. = 0.78 ± 0.01) is slightly higher than that for bleak (0.69 ± 0.03).
Assimilation efficiency [AE = ( P + R ) C ⁻¹] was significantly higher in roach than in bleak. Different levels of AE correlated with differences in relative gut length (gut length as percentage of body length). Due to the shorter relative gut length above a weight of 5 mg, bleak has lower powers of digestion, which may explain lower production rates. These differences in energetic performance between the two species indicate mechanisms leading to niche differentiation in the early life history of the fishes.     

Comparison of growth rate and formation of otolith increments in age-0 red snapper

For wild red snapper Lutjanus campechanus , mean otolith increment deposition rate after marking with oxytetracycline dihydrate (OTC) was daily (0.97 increments day⁻¹) when growth rates were fast (0.63 mm fork length, L _F day⁻¹), but were not daily (0.82 increments day⁻¹) when somatic growth was slow (0.2 mm L _F day⁻¹). For reared larvae ( n =8), increment deposition rates were daily (0.99–1.03 increments day⁻¹), and growth rates ranged from 0.6 to 0.9 mm L _F day⁻¹. Growth rate affected increment deposition rate as a threshold function, i.e. when growth rate was <0.3 mm L _F day⁻¹, deposition was less than daily, but above this level increment deposition did not exceed a daily rate. As growth rates increased increment widths increased. Examination of a sub-sample ( n =8) of the otoliths from the slowest growing wild fish by scanning electron microscopy did not increase increment counts. Because L. campechanus are late spring-early summer spawners, young fish can expect maximum growth due to warm summer temperatures. Thus, daily ageing methods should be well suited to this species.     

Hatching time and growth of Nemurellapictetii (Plecoptera: Nemouridae) in the laboratory and a Lake District stream

SUMMARY. 1. Nemurella pictetii Klapæplek took 2 years to complete its life cycle in both the laboratory and a small stream in the English Lake District.
2. Hatching time (days after oviposition for 10%. 50% and 90% of the eggs to hatch) and hatching period (days between dates for 10% and 90% hatched) decreased with increasing water temperature in the laboratory, and the relationships were well described by a power-law. Estimates of the mean time for 50% hatching in the stream varied between 16 and 31 days after oviposition. depending on temperature.
3. Larval instars numbered fifteen for males and seventeen for females with a constant ratio of 1.18 between successive instars (conformed with Dyar's rule). Larval growth was exponential at four constant temperatures in the laboratory; mean instantaneous growth rates were 0.40±0.01% day⁻¹ at 5.9°C, 0.43±0.01% day⁻¹ at 8.2°C, 0.46±0.01% day⁻¹ at 12. 1°C. 0.56±0.02%day⁻¹ at 19.8°C. No larvae survived after instar XI at 19.8°C.
4. Larval growth was exponential in the stream and was scarcely affected by variations in water temperature (range 4.2 -14.0°C); mean growth rates for three year-classes were 0.41±0.02, 0.43±0.08, 0.54±0.05% day⁻¹. Their similarity to laboratory growth rates under optimum conditions suggests that the availability of resources, such as food and space, was not restricting growth in the stream.     

Compensatory growth and reduced maturation in second sea winter farmed Atlantic salmon following starvation in February and March

Starvation of second sea winter farmed Atlantic salmon through February and March reduced body weight by 0.10% day⁻¹ or 6.0% during the starvation period, whereas fed fish increased body weight by 0.10 day⁻¹ or 5.9%. When fed again during 41 days in April and May, the starved group increased weight by 22.7% (0.55% day⁻¹) compared with a 11.4% (0.28% day⁻¹) gain in the unrestricted control. Ultrasound determination of sex and maturity in late May showed that the incidence of maturation was reduced by 48% among females and 32% among males in the starved group, compared with the unrestricted group.     

Depth equilibration by two predatory cichlid fish from Lake Malawi

The cichlid fish, Haplochromis polystigma and Haplochromis livingstonii are piscivorous ambush predators endemic to Lake Malawi, Africa. Experimentally it was found that large adult H. livingstonii could equilibrate to 38 m, but that juveniles were restricted to shallower depths. Mean depth attained by 8 H. polystigma was 45 m but two individuals exceeded the maximum simulated depth (59 m). H. livingstonii had a mean equilibration rate of 4–7 m day⁻¹ for the first 10 m declining to 2·4 m day⁻¹ thereafter. The mean rate of descent for H. polystigma was 3·8 m day⁻¹ for the first 10 m and 2·8 m day⁻¹ thereafter. The fish were decompressed at a rate of a 20% decrease every 12 h. Although cichlid fishes occur down to 200 m depth in Lake Malawi, experimental and field data indicate that the depth distribution of individual species is restricted and that the restriction is largely related to swimbladder physiology. All cichlid species so far tested equilibrate slowly to depth (2 to 5 m a day) and are not capable of rapid decompression.     

Metabolism of a desert stream

SUMMARY. Rates of photosynthesis and community respiration were determined for benthic assemblages in Sycamore Creek, a Sonoran Desert stream in Arizona. Benthos in this stream can be separated into (1) mats of Cladophora glomerata and associated epiphytes and (2) assemblages of epipelic diatoms and blue-green algae. Community respiration and net photosynthesis were measured for these assemblages using submerged light-dark chambers in situ . Multiple regression analysis was used to predict (1) gross photosynthesis as a function of photosynthetically active radiation, temperature and chlorophyll-α concentration; and (2) community respiration as a function of temperature and biomass.
Calculations suggest that Sycamore Creek is autotrophic during the summer ( P/R = 1.7) and that the rates of gross photosynthesis ( P =8.5 g O₂ m⁻² day⁻¹) and community respiration ( R = 5.1 g O₂ m⁻² day⁻¹) are high for a small stream. Considerable difference exists between the Cladophora mat assemblages, in which mean P is 12.5gO₂m⁻² day⁻¹and the P/R ratio is 2.3, and the epipelic assemblages in which mean P is 4.4 g O₂m⁻² day⁻¹ and P/R is 0.96. The high rate of gross photosynthesis, low litter inputs, high biomass of algae and the intermittent but severe floods that characterize Sycamore Creek indicate that this stream and other similar desert streams are net exporters of organic matter and are, thereby, truly autotrophic stream ecosystems.     

Utilization of several plant proteins by gibel carp (Carassius auratus gibelio)

Six isonitrogenous (gross protein content 35%) and isoenergetic (gross energy content 17 kJ g⁻¹) diets were formulated to investigate the effects of inclusion of plant proteins on the gibel carp ( Carassius auratus gibelio L.). The plant proteins tested were: soybean cake (SBC), potato protein concentrate (PPC), peanut cake (PNC), cottonseed cake (CSC) and rapeseed cake (RSC). Fish meal (FM) was used as control. In each diet, 27% of the protein was supplied by fish meal, and the rest supplied by the plant protein tested. Each diet was fed to three groups of gibel carp for 8 weeks in a recirculation system. Specific growth rate (SGR) in fish fed the control diet was significantly higher than those in the other groups, and SGR in fish fed the PPC was significantly lower than in fish fed other plant proteins. There was no significant difference in SGR among the other groups. Feeding rates were ranked in the order: RSC > CSC > FM > PNC > SBC > PPC. Conversion efficiency was highest in groups fed FM, SBC and PNC, followed by groups fed CSC and RSC, and was lowest in the group fed PPC. The fish fed PPC showed lower protein retention than those fed FM and SBC. FM showed highest energy retention while PPC showed lowest. There was no significant relationship between SGR and intake of digestible protein (g g⁻¹ day⁻¹), digestible lysine (g g⁻¹ day⁻¹), digestible methionine (g g⁻¹ day⁻¹) or digestible total essential amino acids (g g⁻¹ day⁻¹), suggesting that the differences in SGR could not alone account for any of these variables.     

Consumption, growth and evacuation in the Pacific cod, Gadus macrocephalus

Growth of Pacific cod was related to energy consumption (cal g⁻¹ day⁻¹) and was well described by linear equations. Maintenance ration was 11 and 12 cal g⁻¹ day⁻¹ at 4.5 and 6.5° C, respectively. Cod between 200 and 5000 g had similar growth rates when growth was expressed as a function of consumption (cal g⁻¹ day⁻¹). Laboratory consumption of food averaged 0.9 and 1.3% body weight per day at 4.5 and 6.5° C, respectively. At these temperatures growth was 0.34–0.38% body weight day⁻¹.
Maximum stomach volumes equated to approximately 4.7% of body weight with shrimp as prey. At this meal size Pacific cod did not feed the next day. A multiple meal evacuation experiment was used to verify the consumption estimates. A return-to-hunger estimate of the meal size evacuated was 1.5% body weight day⁻¹ at 6.5° C, similar to the 1.3% consumption estimate. For Pacific cod fed a single meal of 1% body weight the estimated instantaneous evacuation rate was 0.63 body weight day⁻¹ at 6.5° C. Meal size markedly affected the evacuation rate.
Measured consumption and growth rates are similar to those of Atlantic cod, Gadus morhua .     

Growth rate, age and size of bonefish from the Gulf of California

Growth rates of cultured first-year bonefish Albula sp. averaged 0·32 mm day⁻¹. Maximum theoretical growth (±95% CI) was 278 (±11·2) mm L _S (sexes combined), confirming that Albula sp. from the Gulf of California grow less than other species in the' vulpes 'complex.     

Life cycle, growth, mortality and production of Ephemerella major Klapalek (Ephemeroptera) in a trout stream in Belgium

SUMMARY. 1. The life cycle of Ephemerella major Klapalek in a chalk trout stream in Belgium took 1 year. Emergence was highly synchronized with a flight period from mid-May to mid-June. Tiny nymphs occurred from June to late August.
2. The mean instantaneous growth rate was high in autumn (3.6% wet wt day⁻¹), very low from November to February (0.8% wet wt day⁻¹) and high until emergence (2.3% wet wt day⁻¹); short day length seemed to be the major factor reducing growth rate during winter.
3. Mortality was close to zero during winter and 1.4—1.7% day⁻¹during other seasons. Total mortality from egg to adult was 99.6%.
4. The annual production was about 9g wet wt m⁻² year⁻¹ and the annual P/ ratio was 7.5. There was good agreement between the production values estimated by four methods. Production rate was highest in May (13 mg wet wt m⁻² day⁻¹) and zero in February.     

Digestion rate, gut passage time and absorption efficiency in the Antarctic spiny plunderfish

The absolute gut evacuation rate (GER) (g day⁻¹) of Harpagifer antarcticus increased with increasing ration mass, fish mass only influenced the absolute GER at a daily ration level of 0·3% wet fish mass (approximately a maintenance ration). The relative GER (% of meal fed day⁻¹) was also affected differently by fish and ration mass depending on the relative ration level being fed; at rations of 0·7% wet fish mass or above the relative GER decreased with increasing fish or ration mass (in such a way that the absolute GER remained constant and unaffected by fish mass). At maintenance (0·3% wet fish mass) rations the relative GER was not affected by fish size or ration mass. Thus, there appears to be a ration threshold above which the digestion physiology alters. Mass-specific GER (% g fish⁻¹ day⁻¹) decreased with increasing fish mass. Within a set relative ration level (% wet fish mass) an increase in fish mass decreased the mass-specific GER. At a fixed ration mass, an increase in fish mass (i.e. a reduction in the ration expressed as % fish mass) resulted in a decrease in mass-specific GER. Gut evaluation time (GET) decreased and absorption efficiency (A) increased with increasing absolute GER. The effect of ration and fish mass on the absolute and relative GER followed the same pattern irrespective of the diet, however the A and GER (% day⁻¹ and g day⁻¹) were higher and the GET shorter when the fish were fed shelled krill rather than amphipods.