共查询到20条相似文献,搜索用时 31 毫秒
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RNA polymerase II acts as an RNA‐dependent RNA polymerase to extend and destabilize a non‐coding RNA
Steven L Ponicsan Linda F Drullinger Jennifer F Kugel James A Goodrich 《The EMBO journal》2013,32(6):781-790
RNA polymerase II (Pol II) is a well‐characterized DNA‐dependent RNA polymerase, which has also been reported to have RNA‐dependent RNA polymerase (RdRP) activity. Natural cellular RNA substrates of mammalian Pol II, however, have not been identified and the cellular function of the Pol II RdRP activity is unknown. We found that Pol II can use a non‐coding RNA, B2 RNA, as both a substrate and a template for its RdRP activity. Pol II extends B2 RNA by 18 nt on its 3′‐end in an internally templated reaction. The RNA product resulting from extension of B2 RNA by the Pol II RdRP can be removed from Pol II by a factor present in nuclear extracts. Treatment of cells with α‐amanitin or actinomycin D revealed that extension of B2 RNA by Pol II destabilizes the RNA. Our studies provide compelling evidence that mammalian Pol II acts as an RdRP to control the stability of a cellular RNA by extending its 3′‐end. 相似文献
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Uncoupling ribosome biogenesis regulation from RNA polymerase I activity during herpes simplex virus type 1 infection 总被引:1,自引:0,他引:1
Stéphane Belin Karine Kindbeiter Sabine Hacot Marie Alexandra Albaret Jean-Xavier Roca-Martinez Gabriel Thérizols Olivier Grosso Jean-Jacques Diaz 《RNA (New York, N.Y.)》2010,16(1):131-140
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Wen L Liang de S Wu LQ Cai F Pan Q Long ZG Dai HP Xia K Xia JH 《Biochemical and biophysical research communications》2008,367(4):846-851
Vector systems to deliver, integrate and express therapeutic genes in host cells are essential for gene therapy. In the present study, we investigated a novel vector system for integration and expression of a transgene. In this system, the transgene expression was driven by an endogenous RNA polymerase I (Pol I) promoter after being integrated into the ribosomal DNA (rDNA) locus. Human coagulation factor IX coding sequence (FIX), with an internal ribosome entry sites element at its leader region, was targeted into the 18S rDNA locus via homologous recombination. FIX protein expression, which was under the control of the endogenous Pol I promoter, was found to be similar to that of a moderate Pol II promoter. The average FIX expression level of the rDNA recombinants was additionally enhanced to that from a strong Pol II promoter as a result of elimination of position effects. Our data suggest the possibility of applying this system in gene therapy for hereditary diseases. 相似文献
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