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1.
The polyene antibiotics, including nystatin, pimaricin, amphotericin, and candicidin, comprise a family of very valuable antifungal
polyketide compounds, and they are typically produced by soil actinomycetes. Previously, using a polyene cytochrome P450 hydroxylase-specific
genome screening strategy, Pseudonocardia autotrophica KCTC9441 was determined to contain genes potentially encoding polyene biosynthesis. Here, sequence information of an approximately
125.7-kb contiguous DNA region in five overlapping cosmids isolated from the P. autotrophica KCTC9441 genomic library revealed a total of 23 open reading frames, which are presumably involved in the biosynthesis of
a nystatin-like compound tentatively named NPP. The deduced roles for six multi-modular polyketide synthase (PKS) catalytic
domains were found to be highly homologous to those of previously identified nystatin biosynthetic genes. Low NPP productivity
suggests that the functionally clustered NPP biosynthetic pathway genes are tightly regulated in P. autotrophica. Disruption of a NPP PKS gene completely abolished both NPP biosynthesis and antifungal activity against Candida albicans, suggesting that polyene-specific genome screening may constitute an efficient method for isolation of potentially valuable
previously identified polyene genes and compounds from various rare actinomycetes widespread in nature. 相似文献
2.
Five strains (LN12, LN14 T, LN15 T, LN16 and LN17 T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive
enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis
of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to
be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14 T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14 T (= BCC 42666 T = NBRC 107780 T = CBS 12095 T). Strain LN15 T was assigned to be Candida chumphonensis sp. nov. (type strain LN15 T = BCC 42667 T = NBRC 107781 T = CBS 12096 T). Strain LN17 T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17 T = BCC 42668 T = NBRC 107782 T = CBS 12097 T). 相似文献
3.
Two strains representing a single novel yeast species were isolated from a flower of Calycoopteris floribunda Lame (SK170 T) and insect frass (ST-122) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were assigned as a single novel Candida species in the Hyphopichia clade for which the name Candida wangnamkhiaoensis sp. nov. is proposed. The type strain is SK170 T=BCC 39604 T=NBRC 106724 T=CBS 11695 T) . 相似文献
4.
The polyene antibiotics, a category that includes nystatin, pimaricin, amphotericin, and candicidin, comprise a family of
very promising antifungal polyketide compounds and are typically produced by soil actinomycetes. The biosynthetic gene clusters
for these polyenes have been previously investigated, revealing the presence of highly similar cytochrome P450 hydroxylase
(CYP) genes. Using polyene CYP-specific PCR screening with several actinomycete genomic DNAs, Pseudonocardia autotrophica was determined to contain a unique polyene-specific CYP gene. Genomic DNA library screening using the polyene-specific CYP
gene probe identified a positive cosmid clone, which contained a DNA fragment of approximately 34.5 kb. The complete sequencing
of this DNA fragment revealed a total of seven complete and two incomplete open reading frames, which were found to be highly
similar, but still unique, when compared to previously known polyene biosynthetic genes. These results suggest that the polyene-specific
screening approach may constitute an efficient method for the isolation of potentially valuable cryptic polyene biosynthetic
gene clusters from various rare actinomycetes. 相似文献
5.
An actinomycete strain, which was designated 172115 T, was isolated from mangrove soil in Shenzhen, China. Strain 172115 T fell within the genus Streptomyces in the 16S rRNA gene tree and could be grouped into this genus based on its chemotaxonomic and morphological data. The strain
shared the highest 16S rRNA gene sequence similarities with Streptomyces lanatus NBRC 12787 T (AB184845) (98.29%) and Streptomyces lucensis NBRC 13056 T (AB184280) (98.26%). The DNA–DNA hybridization values between strain 172115 T and the two most closely related type strains were low enough to justify the assignment of the strain to a novel species.
On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, 172115 T represents a novel species of the genus Streptomyces, for which, the name Streptomyces shenzhenensis sp. nov. is proposed for strain 172115 T (=CCTCC AA 2011001 T=DSM 42034 T). 相似文献
6.
Background Polyenes represent a major class of antifungal agents characterised by the presence of a series of conjugated double bonds
in their planar hydroxylated macrolide ring structure. Despite their general interest, very little is known about the factors
that modulate their biosynthesis. Among these factors, we have recently discovered a new inducing compound (PI-factor) in
the pimaricin producer Streptomyces natalensis, which elicits polyene production in a manner characteristic of quorum sensing. Here, we describe the involvement of an amino-acid
exporter from S. natalensis in modulating the expression of pimaricin biosynthetic genes via secretion of the quorum-sensing pimaricin-inducer PI-factor. 相似文献
7.
In a taxonomic study of yeasts isolated from decaying plant materials submerged in water of mangrove forests in Thailand,
three strains isolated from tree bark (EM33 T), a fallen leaf (EM40 T) and a detached branch (SM56 T) were found to represent three novel yeast species. On the basis of morphological, biochemical, physiological and chemotaxonomic
characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, and the phylogenetic analysis,
the three strains were assigned as three novel Candida species. They were named as Candida chanthaburiensis sp. nov. (type strain EM33 T = BCC 23057 T = NBRC 102176 T = CBS 10926 T), Candida kungkrabaensis sp. nov. (type strain EM40 T = BCC 23060 T = NBRC 102179 T = CBS 10927 T), and Candida suratensis sp. nov. (type strain SM56 T = BCC 25961 T = NBRC 103858 T = CBS 10928 T). 相似文献
8.
[背景]激发子(elicitin)是卵菌(Oomycetes)疫霉和腐霉分泌的可诱发宿主产生免疫反应的小分子化合物.[目的]鉴定紫菜腐霉激发子基因家族,分析其结构特征和在感染宿主过程中可能的作用机制.[方法]运用同源比对法筛查紫菜腐霉NBRC33253基因组中激发子基因家族成员,利用生物信息学工具分析激发子家族的理化性... 相似文献
9.
BackgroundActinobacteria of the genus Nocardia usually live in soil or water and play saprophytic roles, but they also opportunistically infect the respiratory system, skin, and other organs of humans and animals. Primarily because of the clinical importance of the strains, some Nocardia genomes have been sequenced, and genome sequences have accumulated. Genome sizes of Nocardia strains are similar to those of Streptomyces strains, the producers of most antibiotics. In the present work, we compared secondary metabolite biosynthesis gene clusters of type-I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) among genomes of representative Nocardia species/strains based on domain organization and amino acid sequence homology. ResultsDraft genome sequences of Nocardia asteroides NBRC 15531 T, Nocardia otitidiscaviarum IFM 11049, Nocardia brasiliensis NBRC 14402 T, and N. brasiliensis IFM 10847 were read and compared with published complete genome sequences of Nocardia farcinica IFM 10152, Nocardia cyriacigeorgica GUH-2, and N. brasiliensis HUJEG-1. Genome sizes are as follows: N. farcinica, 6.0 Mb; N. cyriacigeorgica, 6.2 Mb; N. asteroides, 7.0 Mb; N. otitidiscaviarum, 7.8 Mb; and N. brasiliensis, 8.9 - 9.4 Mb. Predicted numbers of PKS-I, NRPS, and PKS-I/NRPS hybrid clusters ranged between 4–11, 7–13, and 1–6, respectively, depending on strains, and tended to increase with increasing genome size. Domain and module structures of representative or unique clusters are discussed in the text. ConclusionWe conclude the following: 1) genomes of Nocardia strains carry as many PKS-I and NRPS gene clusters as those of Streptomyces strains, 2) the number of PKS-I and NRPS gene clusters in Nocardia strains varies substantially depending on species, and N. brasiliensis strains carry the largest numbers of clusters among the species studied, 3) the seven Nocardia strains studied in the present work have seven common PKS-I and/or NRPS clusters, some of whose products are yet to be studied, and 4) different N. brasiliensis strains have some different gene clusters of PKS-I/NRPS, although the rest of the clusters are common within the N. brasiliensis strains. Genome sequencing suggested that Nocardia strains are highly promising resources in the search of novel secondary metabolites. Electronic supplementary materialThe online version of this article (doi: 10.1186/1471-2164-15-323) contains supplementary material, which is available to authorized users. 相似文献
10.
【背景】耐酸乳杆菌(Lactobacillus acetotolerans)是白酒发酵过程中的优势乳酸菌,对白酒发酵具有重要作用。L. acetotolerans G10是分离自芝麻香型白酒发酵酒醅的一株能够利用多种碳源的菌株。【目的】基于全基因组测序,解析菌株G10多碳源利用机制。【方法】通过三代测序平台Oxford Nanopore完成菌株G10全基因组测序,分别利用Circlator和Prodigal对测序数据进行组装和基因预测;通过细菌基因组分析工具(bacterial pan genome analysis tool,BPGA)进行泛基因组分析。【结果】G10能够利用22种糖类及糖类衍生物,其全基因组大小为1 627 828 bp,含有1 878个编码基因;基于Koyto Encyclopedia of Genes and Genomes (KEGG)数据库注释获得292个碳源代谢相关基因,基于Carbohydrate-Active Enzymes (CAZy)数据库注释获得44个CAZy家族的编码基因。与其他发酵食品来源的耐酸乳杆菌相比,G10基因组最小,但其总基因数量以及... 相似文献
11.
PurposeA polyphasic analysis was performed on a novel bacterium, designated strain KMU-143T, which was isolated from seawater collected in the Republic of Korea. MethodsA novel marine bacterium KMU-143T was analyzed and described using a polyphasic taxonomic method including 16S rRNA gene sequence analysis, DNA–DNA hybridization, and physiological, biochemical, and chemotaxonomic analyses. ResultsStrain KMU-143T was Gram-stain-negative, strictly aerobic, oval-shaped, non-motile, and chemoorganoheterotrophic. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that the novel marine bacterium belongs to the family Rhodobacteraceae, of the class Alphaproteobacteria, and that it possessed the highest (97.1%) sequence similarity with Sulfitobacter pontiacus ChLG 10T and Sulfitobacter undariae W-BA2T. DNA–DNA relatedness values between strains KMU-143T, S. pontiacus JCM 21789T, and S. undariae KCTC 42200T were less than 70%. The major isoprenoid quinone of the novel isolate was ubiquinone-10 (Q-10) and the major (> 10%) cellular fatty acids were C16:0 and C18:1 ω7c. The genomic DNA G+C content of strain KMU-143T was 56.1 mol%. The polar lipid profile of the strain KMU-143T was found to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, and two unidentified lipids. ConclusionBased on the discriminative phylogenetic position and combination of genotypic and phenotypic properties, the strain is considered to represent a new species of the genus Sulfitobacter for which the name Sulfitobacter salinus sp. nov. is proposed. The type strain of S. salinus sp. nov. is KMU-143T (= KCCM 90322T = NBRC 113459T). 相似文献
12.
Three strains (LM008 T, LM068 and LM078 T), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008 T was assigned to be Candida sirachaensis sp. nov. (type strain LM008 T = BCC 47628 T = NBRC 108605 T CBS 12094 T) in the Starmerella clade. Two strains (LM068 and LM078 T) represent a single species in the Lodderomyces- Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078 T = BCC 47632 T = NBRC 108895 T = CBS 12318 T. 相似文献
13.
A Gram-negative, aerobic, rod shaped, non-spore-forming bacterial strain, designated Dae08 T, was isolated from sediment of the stream near Daechung dam in South Korea, and was characterized in order to determine its
taxonomic position, using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Dae08 T belongs to the family Xanthomonadaceae of the Gammaproteobacteria, and is related to Lysobacter brunescens ATCC 29482 T (97.3%). The phylogenetic distances from any other species with validly published names within the genus Lysobacter were greater than 3.7%. The G+C contents of the genomic DNA of strain Dae08 T was 69.3 mol%. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with iso-C 15:0, iso-C 17:1, ω9 c, iso-C 17:0, iso-C 16:0, and iso-C 11:0 3-OH as the major acids supported the affiliation of strain Dae08 T to the genus Lysobacter. DNA-DNA relatedness between strain Dae08 T and its phylogenetically closest neighbour was 28%. On the basis of its phenotypic properties and phylogenetic distinctiveness,
strain Dae08 T (= KCTC 12600 T) should be classified in the genus Lysobacter as the novel species, for which the name Lysobacter daecheongensis sp. nov. is proposed. 相似文献
14.
A halotolerant bacterium, strain SMB34 T, was isolated from a naphthalene-utilizing bacterial consortium obtained from primitive technogeneous soil (Verkhnekamsk
salt deposit, Perm region, Russia) by enrichment procedure. The strain itself was unable to degrade naphthalene and grew at
NaCl concentrations up to 11% (w/v). The 16S rRNA-based phylogenetic analysis showed that the strain belongs to the genus
Thalassospira. The DNA-DNA hybridization values between SMB34 T and the type strains of phylogeneti-cally closest species ( T. xiamenensis, T. profundimaris and T. tepidiphila) did not exceed 50%. The novel strain could be distinguished from the above species by the cell motility, MALDI/TOF mass
spectra of whole cells and a range of physiological and biochemical characteristics. SMB34 T also considerably differs from the recently described species T. xianhensis, with the most striking differences in the DNA G + C content (53. ± 1.0 vs. 61.2 ± 1.0 mol %) and predominant ubiquinones
(Q-10 vs. Q-9). The data obtained suggest strain SMB34 T (=VKM B-2527 T = NBRC 106175 T), designated as the type strain, represents a novel species, named Thalassospira permensis sp. nov. 相似文献
15.
A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11 T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11 T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807 T (99.38%) and Streptomyces mirabilis JCM 4791 T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807 T and Streptomyces siamensis NBRC 108799 T (98.62%). The menaquinones were identified as MK-9(H 8), MK-9(H 6) and MK-9(H 4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C 15:0, iso-C 16:0, C 16:0 and C 15:0. However, multilocus sequence analysis based on five house-keeping genes ( atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11 T (=DSM 42165 T=CGMCC 4.7198 T) as the type strain. 相似文献
16.
A novel actinomycete, designated strain NEAU-ycm1 T, was isolated from an edible Chinese black ant ( Polyrhachis vicina Roger) and characterized with a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence show that the novel isolate belongs to the genus Streptomyces and forms a separate subclade. The closest phylogenetic relatives were identified as the type strains of Streptomyces intermedius NBRC 13049 T (97.74 %), Streptomyces aureoverticillatus NRRL B-3326 T (97.69 %), Streptomyces rutgersensis NBRC 12819 T (97.68 %), Streptomyces gougerotii NBRC 3198 T (97.68 %) and Streptomyces diastaticus subsp . diastaticus NBRC 3714 T (97.68 %). Similarities to other type strains of the genus Streptomyces were lower than 97.55 %. A comparison between strain NEAU-ycm1 T and the closest related Streptomyces type strains revealed that it is different from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that NEAU-ycm1 T (=CGMCC 4.7094 T = DSM 42102 T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces polyrhachii sp. nov. is proposed. 相似文献
17.
A novel Gram-negative and rod-shaped bacterium, designated N8 T, was isolated from tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that N8 T strain is associated with the family Phyllobacteriaceae: two uncultured clones (98.4 and 99.8% 16S rRNA gene sequence similarity) and the genus Mesorhizobium (≤97.0%). The novel strain formed a separate clade with uncultured clones in the phylogenetic tree based on 16S rRNA gene
sequences. Cellular fatty acid profiles predominately comprised C 18:1
ω7 c and C 19:0 cyclo ω8 c. The major isoprenoid quinone is ubiquinone-10 and genomic DNA G+C content is 53.4 mol%. The polyphasic taxonomic study indicates
that the novel strain N8 T represents a novel species of the new genus in the family Phyllobacteriaceae, named Aliihoeflea aestuarii. The type strain is N8 T (= KCTC 22052 T= JCM 15118 T= DSM 19536 T). 相似文献
18.
A novel actinobacterium, strain SK68, was isolated from the rhizosphere of peanut plant and its salinity stress alleviation ability was studied using tomato ( Solanum lycopersicum cv. Micro-Tom) plants. Based on 16S rDNA based phylogenetic analysis, strain SK68 has been identified as a Streptomyces sp. Strain SK68 had branched substrate mycelium bearing smooth surfaced spores and the spore colour is brownish grey on ISP4 medium. It exhibited enzyme activities such as xylanase, cellulase, amylase, and pectinase and degraded hypoxanthine, casein, and L-tyrosine. The strain SK68 differed in its banding pattern in BOX-PCR and RAPD fingerprinting compared to the closely matching type strains Streptomyces erythrochromogenes NBRC 3304 T (AB184746), S. flavotricini NBRC 12770 T (AB184132), S. racemochromogenes NBRC 12906 T (AB184235), and S. polychromogenes NBRC 13072 T (NR041109). Strain SK68 was evaluated for its salinity stress-alleviating activity in tomato plants with 180 mmol/L NaCl under gnotobiotic condition. A significant increase in plant biomass was observed in strain SK68-inoculated tomato plants under salt stress compared to control and salt-stressed non-inoculated plants. 相似文献
19.
A pink-coloured, salt- and alkali-tolerant planctomycetal strain (JC658T) with oval to pear-shaped, motile, aerobic, Gram-negative stained cells was isolated from a marine sponge, Pseudoceratina sp. Strain JC658T shares the highest 16S rRNA gene sequence identity with Maioricimonas rarisocia Mal4T (<?89.2%) in the family Planctomycetaceae. The genomic analysis of the new strain indicates its biotechnological potential for the production of various industrially important enzymes, notably sulfatases and carbohydrate-active enzymes (CAZymes), and also potential antimicrobial compounds. Several genes encoding restriction-modification (RM) and CRISPR-CAS systems are also present. NaCl is obligate for growth, of which strain JC658T can tolerate a concentration up to 6% (w/v). Optimum pH and temperature for growth are 8.0 (range 7.0–9.0) and 25 ºC (range 10–40 °C), respectively. The major respiratory quinone of strain JC658T is MK6. Major fatty acids are C16:1ω7c/C16:1ω6c, C18:0 and C16:0. Major polar lipids are phosphatidylcholine, phosphatidyl-dimethylethanolamine and phosphatidyl-monomethylethanolamine. The genomic size of strain JC658T is 7.36 Mb with a DNA G?+?C?content of 54.6 mol%. Based on phylogenetic, genomic (ANI, AAI, POCP, dDDH), chemotaxonomic, physiological and biochemical characteristics, we conclude that strain JC658T belongs to a novel genus and constitutes a novel species within the family Planctomycetaceae, for which we propose the name Thalassoroseus pseudoceratinae gen. nov., sp. nov. The novel species is represented by the type strain JC658T (=?KCTC 72881 T?=?NBRC 114371 T). 相似文献
20.
A novel actinomycete strain, designated YIM 45720 T, was isolated from a Cephalotaxus fortunei rhizophere soil sample collected from Yunnan Province, southwest China. The strain formed well-differentiated aerial and
substrate mycelia. Chemotaxonomically, it contained LL-diaminopimelic acid in the cell wall. The cell-wall sugars contained
ribose, mannose, and galactose with traces of glucose and xylose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylcholine and phosphatidylinositol. MK-9 (H 8) was the predominant menaquinone. The major fatty acids (>10%) were iso-C 16:0, iso-C 15:1 and anteiso-C 15:0. The G + C content of the DNA was 70 mol%. Phylogenetic analysis data based on 16S rRNA gene sequence showed that strain YIM 45720 T formed a distinct branch with the type strain of Streptomyces scabrisporus JCM 11712 T within the genus Streptomyces. On the basis of the phenotypic and genotypic characteristics, strain YIM 45720 T (=DSM 41883 T = CCTCC AA 206006 T) is proposed as the type strain of a novel species, Streptomyces serianimatus sp. nov. 相似文献
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