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Two Distinct Hemolytic Activities in Xenorhabdus nematophila Are Active against Immunocompetent Insect Cells 总被引:6,自引:0,他引:6
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Julien Brillard Carlos Ribeiro Noël Boemare Michel Brehlin Alain Givaudan 《Applied microbiology》2001,67(6):2515-2525
Xenorhabdus spp. and Photorhabdus spp. are major insect bacterial pathogens symbiotically associated with nematodes. These bacteria are transported by their nematode hosts into the hemocoel of the insect prey, where they proliferate within hemolymph. In this work we report that wild strains belonging to different species of both genera are able to produce hemolysin activity on blood agar plates. Using a hemocyte monolayer bioassay, cytolytic activity against immunocompetent cells from the hemolymph of Spodoptera littoralis (Lepidoptera: Noctuidae) was found only in supernatants of Xenorhabdus; none was detected in supernatants of various strains of Photorhabdus. During in vitro bacterial growth of Xenorhabdus nematophila F1, two successive bursts of cytolytic activity were detected. The first extracellular cytolytic activity occurred when bacterial cells reached the stationary phase. It also displayed a hemolytic activity on sheep red blood cells, and it was heat labile. Among insect hemocyte types, granulocytes were the preferred target. Lysis of hemocytes by necrosis was preceded by a dramatic vacuolization of the cells. In contrast the second burst of cytolytic activity occurred late during stationary phase and caused hemolysis of rabbit red blood cells, and insect plasmatocytes were the preferred target. This second activity is heat resistant and produced shrinkage and necrosis of hemocytes. Insertional inactivation of flhD gene in X. nematophila leads to the loss of hemolysis activity on sheep red blood cells and an attenuated virulence phenotype in S. littoralis (A. Givaudan and A. Lanois, J. Bacteriol. 182:107–115, 2000). This mutant was unable to produce the early cytolytic activity, but it always displayed the late cytolytic effect, preferably active on plasmatocytes. Thus, X. nematophila produced two independent cytolytic activities against different insect cell targets known for their major role in cellular immunity. 相似文献
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New Insights into the Colonization and Release Processes of Xenorhabdus nematophila and the Morphology and Ultrastructure of the Bacterial Receptacle of Its Nematode Host, Steinernema carpocapsae
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Holly Snyder S. Patricia Stock Sam-Kyu Kim Yolanda Flores-Lara Steven Forst 《Applied microbiology》2007,73(16):5338-5346
We present results from epifluorescence, differential interference contrast, and transmission electron microscopy showing that Xenorhabdus nematophila colonizes a receptacle in the anterior intestine of the infective juvenile (IJ) stage of Steinernema carpocapsae. This region is connected to the esophagus at the esophagointestinal junction. The process by which X. nematophila leaves this bacterial receptacle had not been analyzed previously. In this study we monitored the movement of green fluorescent protein-labeled bacteria during the release process. Our observations revealed that Xenorhabdus colonizes the distal region of the receptacle and that exposure to insect hemolymph stimulated forward movement of the bacteria to the esophagointestinal junction. Continued exposure to hemolymph caused a narrow passage in the distal receptacle to widen, allowing movement of Xenorhabdus down the intestine and out the anus. Efficient release of both the wild type and a nonmotile strain was evident in most of the IJs incubated in hemolymph, whereas only a few IJs incubated in nutrient-rich broth released bacterial cells. Incubation of IJs in hemolymph treated with agents that induce nematode paralysis dramatically inhibited the release process. These results suggest that bacterial motility is not required for movement out of the distal region of the receptacle and that hemolymph-induced esophageal pumping provides a force for the release of X. nematophila out of the receptacle and into the intestinal lumen. 相似文献
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《Journal of Asia》2022,25(4):101997
Xenorhabdus and Photorhabdus are two bacterial genera specifically symbiotic to Steinernema and Heterorhabditis, which are the entomopathogenic nematode genera, respectively. These bacteria are well known to produce potent secondary metabolites suppressing insect immune responses. This study aimed to develop a potent microbial insecticide against the onion thrips, Thrips tabaci, using the bacterial metabolites. Among the chemical insecticides that have been used to control the thrips, spinosad was highly effective against both larvae and adults of T. tabaci. Three different entomopathogenic fungi were also effective to kill the thrips. However, the fungal virulence was much less than the control efficacy of the chemical insecticide, spinosad. To enhance the fungal virulence of Beauveria bassiana (Bb), the bacterial culture broth of Xenorhabdus/Photorhabdus was added to suppress the thrips immune defense. Among six different bacterial species, X. hominickii (Xh) produced highly potent metabolites to enhance the fungal virulence. Indeed, four different bacterial metabolites (GameXPeptide, benzylideneacetone, oxindole, and 3-ethoxy-4-methoxyphenol) of the bacteria suppressed the gene expressions of an antimicrobial peptide, lysozyme, which was highly inducible to the fungal infection. To optimize the mixture ratio of fungal and bacterial pathogens, the fungal conidia and bacterial culture broth were freeze-dried and mixed in different ratios. Laboratory and field assays showed that a mixture spray of freeze-dried Xh culture broth (3 g) and Bb conidia (1.17 × 109 conidia) in a liter was effective to control T. tabaci infesting welsh onion. 相似文献
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《Journal of Asia》2022,25(3):101949
Bacteria have a particular strategy to invade the host immune system by forming an undetectable dormant state that may resuscitate and cause disease even after inhabiting for years in a host body. Several mechanisms are known to be responsible for bacterial dormancy, among them the hipBA toxin-antitoxin (TA) system which was initially identified in Escherichia coli. Here we explore the genomic distribution and functional association of hipBA TA homologs from an entomopathogenic bacterium Xenorhabdus nematophila. This bacterium is a symbiotic model with the nematode Steinernema carpocapsae. We found that HipA toxin homologs are more closely related than HipB antitoxins and have satisfactory adenine (for HipA homologs) and nucleic acid (for HipB homologs) ligand partners with a typical TA interaction network that may promote the X. nematophila towards a stringent response to form the dormant state. Such homologs distribution is an inclusion in the current TA repertoire of X. nematophila. 相似文献
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Swati Singh David Orr Emmanuel Divinagracia Joseph McGraw Kellen Dorff Steven Forst 《Applied and environmental microbiology》2015,81(2):754-764
Xenorhabdus nematophila engages in a mutualistic partnership with the nematode Steinernema carpocapsae, which invades insects, migrates through the gut, and penetrates into the hemocoel (body cavity). We showed previously that during invasion of Manduca sexta, the gut microbe Staphylococcus saprophyticus appeared transiently in the hemocoel, while Enterococcus faecalis proliferated as X. nematophila became dominant. X. nematophila produces diverse secondary metabolites, including the major water-soluble antimicrobial xenocoumacin. Here, we study the role of X. nematophila antimicrobials in interspecies competition under biologically relevant conditions using strains lacking either xenocoumacin (ΔxcnKL strain), xenocoumacin and the newly discovered antibiotic F (ΔxcnKL:F strain), or all ngrA-derived secondary metabolites (ngrA strain). Competition experiments were performed in Grace''s insect medium, which is based on lepidopteran hemolymph. S. saprophyticus was eliminated when inoculated into growing cultures of either the ΔxcnKL strain or ΔxcnKL:F strain but grew in the presence of the ngrA strain, indicating that ngrA-derived antimicrobials, excluding xenocoumacin or antibiotic F, were required to eliminate the competitor. In contrast, S. saprophyticus was eliminated when coinjected into M. sexta with either the ΔxcnKL or ngrA strain, indicating that ngrA-derived antimicrobials were not required to eliminate the competitor in vivo. E. faecalis growth was facilitated when coinjected with either of the mutant strains. Furthermore, nematode reproduction in M. sexta naturally infected with infective juveniles colonized with the ngrA strain was markedly reduced relative to the level of reproduction when infective juveniles were colonized with the wild-type strain. These findings provide new insights into interspecies competition in a host environment and suggest that ngrA-derived compounds serve as signals for in vivo nematode reproduction. 相似文献
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Eicosanoids mediate insect immune responses, especially against bacterial infection. Phospholipase A2 (PLA2) catalyzes the committed step of the eicosanoid biosynthesis pathway. Three PLA2 inhibitors have been identified from metabolites of an entomopathogenic bacterium, Xenorhabdus nematophila: benzylideneacetone (BZA), Pro-Tyr (PY), and acetylated Phe-Gly-Val (Ac-FGV). Interestingly, they share benzenepropane as a core chemical structure. We analyzed the functional significance of the core structure using structural derivatives. Removing a phenyl ring from PY resulted in significant loss of the PLA2 inhibitory activity, as seen in a Pro-Ala derivative. Though the p-hydroxyl group was not critical in PY as seen in Pro-Phe derivative, its addition to BZA resulted in significant loss of inhibitory activity. Some alterations of structures other than the core structure increased PLA2-inhibitory activity in some derivatives, including Ala-Tyr (AY) and Phe-Gly-Val (FGV) derivatives. Using these selected derivatives, we further analyzed synergistic effects on pathogenicity of Bacillus thuringiensis (Bt) against the second instar larvae of Plutella xylostella. These two derivatives significantly enhanced the Bt pathogenicity. This study introduces two novel compounds that inhibit PLA2 and suggests their application in combination with Bt to control P. xylostella. 相似文献
8.
Identification and Functional Characterization of a Xenorhabdus nematophila Oligopeptide Permease 总被引:3,自引:0,他引:3
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The bacterium Xenorhabdus nematophila is a mutualist of Steinernema carpocapsae nematodes and a pathogen of insects. Presently, it is not known what nutrients the bacterium uses to thrive in these host environments. In other symbiotic bacteria, oligopeptide permeases have been shown to be important in host interactions, and we therefore sought to determine if oligopeptide uptake is essential for growth or symbiotic functions of X. nematophila in laboratory or host environments. We identified an X. nematophila oligopeptide permease (opp) operon of two sequential oppA genes, predicted to encode oligopeptide-binding proteins, and putative permease-encoding genes oppB, oppC, oppD, and oppF. Peptide-feeding studies indicated that this opp operon encodes a functional oligopeptide permease. We constructed strains with mutations in oppA1, oppA2, or oppB and examined the ability of each mutant strain to grow in a peptide-rich laboratory medium and to interact with the two hosts. We found that the opp mutant strains had altered growth phenotypes in the laboratory medium and in hemolymph isolated from larval insects. However, the opp mutant strains were capable of initiating and maintaining both mutualistic and pathogenic host interactions. These data demonstrate that the opp genes allow X. nematophila to utilize peptides as a nutrient source but that this function is not essential for the existence of X. nematophila in either of its host niches. To our knowledge, this study represents the first experimental analysis of the role of oligopeptide transport in mediating a mutualistic invertebrate-bacterium interaction. 相似文献
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Abstract An entomopathogenic bacterium, Xenorhabdus nematophila, has been known to induce significant immunosuppression of target insects by inhibiting immune‐associated phospholipase A2 (PLA2), which subsequently shuts down biosynthesis of eicosanoids that are critical in immune mediation in insects. Some metabolites originated from the bacterial culture broth have been identified and include benzylideneacetone, proline‐tyrosine and acetylated phenylalanine‐glycine‐valine, which are known to inhibit enzyme activity of PLA2 extracted from hemocyte and fat body. This study tested their effects on digestive PLA2 of the beet armyworm, Spodoptera exigua. Young larvae fed different concentrations of the three metabolites resulted in significant adverse effects on larval development even at doses below 100 μg/mL. In particular, they induced significant reduction in digestive efficiency of ingested food. All three metabolites significantly inhibited catalytic activity of digestive PLA2 extracted from midgut lumen of the fifth instar larvae at a low micromolar range. These results suggest that the inhibitory activities of the three bacterial metabolites on digestive PLA2 of S. exigua midgut may explain some of their oral toxic effects. 相似文献
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Stability and Activities of Antibiotics Produced during Infection of the Insect Galleria mellonella by Two Isolates of Xenorhabdus nematophilus 总被引:6,自引:2,他引:4
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Philip W. Maxwell Genhui Chen John M. Webster Gary B. Dunphy 《Applied microbiology》1994,60(2):715-721
Xenorhabdus nematophilus subsp. dutki, an entomopathogenic bacterium, is vectored by steinernematid nematodes into insects, where it produces broad-spectrum antibiotics. The use of the nematode-bacterium complex against soil-dwelling pest insects could introduce antibiotics into the soil via the dead insect fragments during the emergence phase of the nematodes. Studies on the stability and activities of these antibiotics produced in the insect Galleria mellonella may contribute to assessing the possible impact of antibiotics on soil bacteria. Two isolates of X. nematophilus subsp. dutki (isolates GI and SFU) produced xenocoumacins 1 and 2 in cadavers of G. mellonella larvae in a 1:1 ratio. Total xenocoumacin 1 and 2 production was 800 ng/200 mg (wet weight) of insect tissue for the GI isolate. Antibiotic activity of water extracts from insects that had been infected with X. nematophilus was stable at 60°C for 1 h and after repeated freeze-thaw cycles. The antibiotic titer of extracts held at 27°C declined by day 10. The spectrum of bacterial species killed by antibiotics produced in insect cadavers varied with the isolate of X. nematophilus. Levels of antibiotic activity were greater in vivo than in tryptic soy broth, which may represent a nutrient effect. The bacterial isolate, culture condition, and presence of nematodes influenced the total antibiotic production in vivo. However, the levels of activity were not correlated with bacterial levels in the different growth environments. Insect cadavers with antibiotic activity transiently lowered the numbers of the bacteria in the soil, the extent of decline varying with the strain of X. nematophilus and the time of sampling. 相似文献
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Overproduction of fungal ribotoxin α-sarcin in Escherichia coli: generation of an active immunotoxin
《Gene》1997,190(1):31-35
α-Sarcin is a ribonucleolytic protein secreted by the mold Aspergillus giganteus. DNA encoding α-sarcin was isolated from the host and cloned into T7 promoter based E. coli expression vectors. Using bacterial outer membrane protein A (OmpA) signal sequence, properly processed recombinant (re-) protein was secreted into the culture medium while in the absence of a signal sequence protein remained insoluble in the bacterial inclusion bodies. The re-α-sarcin was purified to homogeneity by simple chromatographic techniques both from the insoluble and soluble sources with respective yields of 40–50 μg/ml and 2–3 μg/ml. The re-ribotoxin was functionally as active as the native toxin and preserved its specificity. The re-α-sarcin was used in the construction of an active immunotoxin targeted at the human cancer cells overexpressing transferrin receptor (TFR). 相似文献
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Anna K. Picman 《Biochemical Systematics and Ecology》1983,11(3):183-186
Two sesquiterpene lactones, helenin and isohelenin, were examined for their activity against 16 species of fungi. These compounds varied greatly in their antifungal activities. At concentrations of 10 μg/ml, the lactones strongly inhibited the growth of Microsporum cookei, Trichophyton mentagrophytes and Trichothecium roseum, while other fungi were only inhibited by considerably higher levels (100–1000 μg/ml). It is suggested that these secondary plant metabolites might be of potential use as antifungal agents, especially if their activity and specificity could further be enhanced through modifications in their chemical structure. 相似文献
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Yu. A. Nikolaev V. K. Plakunov N. A. Voronina N. V. Nemtseva A. O. Plotnikov O. A. Gogoleva M. E. Murav’eva G. V. Ovechkina 《Microbiology》2008,77(1):78-83
The growth characteristics of an algo-bacterial community (Chlamydomonas reinhardtii and bacterial satellites) were studied, as well as the mechanism and patterns of bacterial effect on algae. Four strains of predominant bacteria were isolated and partially characterized. They were assigned to the following taxa: Rhodococcus terrea, Micrococcus roseus, and Bacillus spp. A pure culture of the alga under study was obtained by plating serial dilutions on agarized media. Within the algo-bacterial association, the alga had a higher growth rate (0.76 day?1) and yield (60 μg chlorophyll/ml culture) than in pure cultures (0.4 day?1 and 10 μg chlorophyll/ml culture, respectively). The viability of the algal cells within the association was retained longer than in pure culture. Among the isolated bacterial satellites, strains B1 and Y1, assigned to the species Rhodococcus terrae, had the highest stimulatory effect on algal growth. The culture liquid of bacteria incubated under the conditions not permitting growth stimulated algal growth; the culture liquid of actively growing bacteria had an opposite effect. 相似文献
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Stages of Infection during the Tripartite Interaction between Xenorhabdus nematophila, Its Nematode Vector, and Insect Hosts 总被引:5,自引:0,他引:5
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Mathieu Sicard Karine Brugirard-Ricaud Sylvie Pags Anne Lanois Noel E. Boemare Michel Brehlin Alain Givaudan 《Applied microbiology》2004,70(11):6473-6480
Bacteria of the genus Xenorhabdus are mutually associated with entomopathogenic nematodes of the genus Steinernema and are pathogenic to a broad spectrum of insects. The nematodes act as vectors, transmitting the bacteria to insect larvae, which die within a few days of infection. We characterized the early stages of bacterial infection in the insects by constructing a constitutive green fluorescent protein (GFP)-labeled Xenorhabdus nematophila strain. We injected the GFP-labeled bacteria into insects and monitored infection. We found that the bacteria had an extracellular life cycle in the hemolymph and rapidly colonized the anterior midgut region in Spodoptera littoralis larvae. Electron microscopy showed that the bacteria occupied the extracellular matrix of connective tissues within the muscle layers of the Spodoptera midgut. We confirmed the existence of such a specific infection site in the natural route of infection by infesting Spodoptera littoralis larvae with nematodes harboring GFP-labeled Xenorhabdus. When the infective juvenile (IJ) nematodes reached the insect gut, the bacterial cells were rapidly released from the intestinal vesicle into the nematode intestine. Xenorhabdus began to escape from the anus of the nematodes when IJs were wedged in the insect intestinal wall toward the insect hemolymph. Following their release into the insect hemocoel, GFP-labeled bacteria were found only in the anterior midgut region and hemolymph of Spodoptera larvae. Comparative infection assays conducted with another insect, Locusta migratoria, also showed early bacterial colonization of connective tissues. This work shows that the extracellular matrix acts as a particular colonization site for X. nematophila within insects. 相似文献
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《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1998,709(1):97-104
A simple, accurate, precise, and versatile high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of three quinolone antibiotics in Mueller–Hinton broth. The fluoroquinolone agents studied were ciprofloxacin, ofloxacin, and sparfloxacin; other quinolone agents have been identified using this method but not validated in this matrix (levofloxacin, clinafloxacin, temafloxacin, and trovafloxacin). In addition, several other biological growth mediums have been investigated (human serum, human urine, Todd–Hewitt growth media, Ensure enteral feeding solution, and Haemophilus growth media). This method uses UV detection (280 nm), a simple, one-step protein precipitation extraction, and separation using a C18 column with an isocratic, ion-pairing mobile phase. An appropriate internal standard was obtained by using another quinolone antibiotic of differing retention time. The calibration curves were linear (r2≥0.999) over a concentration range of 0.0625–20.0 μg/ml with a lower limit of quantification of 0.1 μg/ml. The intra-day and inter-day coefficients of variation were less than 15%. 相似文献
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The gammaproteobacterium Xenorhabdus nematophila engages in a mutualistic association with an entomopathogenic nematode and also functions as a pathogen toward different insect hosts. We studied the role of the growth-phase-regulated outer membrane protein OpnS in host interactions. OpnS was shown to be a 16-stranded β-barrel porin. opnS was expressed during growth in insect hemolymph and expression was elevated as the cell density increased. When wild-type and opnS deletion strains were coinjected into insects, the wild-type strain was predominantly recovered from the insect cadaver. Similarly, an opnS-complemented strain outcompeted the ΔopnS strain. Coinjection of the wild-type and ΔopnS strains together with uncolonized nematodes into insects resulted in nematode progeny that were almost exclusively colonized with the wild-type strain. Likewise, nematode progeny recovered after coinjection of a mixture of nematodes carrying either the wild-type or ΔopnS strain were colonized by the wild-type strain. In addition, the ΔopnS strain displayed a competitive growth defect when grown together with the wild-type strain in insect hemolymph but not in defined culture medium. The ΔopnS strain displayed increased sensitivity to antimicrobial compounds, suggesting that deletion of OpnS affected the integrity of the outer membrane. These findings show that the OpnS porin confers a competitive advantage for the growth and/or the survival of X. nematophila in the insect host and provides a new model for studying the biological relevance of differential regulation of porins in a natural host environment.The bacterium Xenorhabdus nematophila forms a mutualistic association with the entomopathogenic nematode Steinernema carpocapsae (2). The nonfeeding infective juvenile form of the nematode (IJ) exists in the soil and carries the bacteria in a specialized receptacle region in the anterior intestine (4, 39). The IJ invades susceptible insect species and enters the hemocoel, where exposure to insect hemolymph stimulates the movement of bacteria down the intestine and out of the anus (36, 39). Together, the nematode and bacteria kill the insect host. X. nematophila not only helps to kill the insect but also promotes bioconversion of host macromolecules and tissues to provide nutrients for nematode reproduction and secretes diverse antimicrobial products to suppress competition for the nutrient resources of the insect cadaver (11, 13, 18, 19, 38). In turn, the nematode vectors X. nematophila to new insect hosts and protects it from the competitive environment of the soil. Colonization of the nematode receptacle is predominantly a monoculture process that is initiated by a single cell followed by bacterial proliferation (24, 39). The level of colonization varies from a few cells to several hundreds per nematode and is higher in nematodes reproducing in insects than on bacterial lawns, suggesting that the insect environment provides additional nutrients for bacterial growth (16, 39).Hydrophilic nutrients and antibiotics passively diffuse across the outer membrane of gram-negative bacteria through general porins and substrate-specific channels (17, 29). The most extensively studied general porins, OmpF and OmpC of Escherichia coli (30), are 16-stranded β-barrel proteins that are reciprocally regulated by changes in external osmolarity (12, 21, 41). Although the flow rate through OmpF is greater than OmpC (28), comparison of the resolved crystal structures does not reveal significant physiochemical differences between the two porins (3). The biological significance of the differential regulation of porins with distinct functional properties remains unclear. The major outer membrane protein of X. nematophila, OpnP, was shown to be produced at high levels in exponentially growing cells and is a homologue of OmpF and OmpC (14). OpnP production was not affected by changes in medium osmolarity, and the flow rate measured for the OpnP porin was more similar to the restrictive porin OmpC than to the more permissive OmpF porin (3). As cells transitioned to stationary phase, de novo synthesis of OpnP decreased, while the synthesis of the outer membrane protein, designated OpnS, increased (15, 22).Porin function and regulation have been studied in both pathogenic and symbiotic bacteria. In Vibrio cholerae two well-studied porins, OmpU and OmpT, that possess distinct functional properties have been shown to be differentially regulated (37). OmpU confers resistance to sodium deoxycholate (DC), a major component of bile, as well as polymixin B, detergents, and antimicrobial peptides, while the expression of OmpT alone sensitizes the cell to DC (26, 33). OmpU was thought to be expressed when V. cholerae colonizes the intestine, suggesting that it was required for host colonization (33); however, subsequent findings indicated that neither OmpU nor OmpT were essential for intestinal colonization (34). Recent findings indicated that OmpU may sense membrane perturbations and activate DegS which in turn modulates σE activity (25, 26). In the symbiotic bacterium Vibrio fischeri the deletion of ompU was shown to reduce the efficiency of colonization of the light organ of the Euprymna scolopes squid and increase sensitivity to bile, antimicrobial peptides, and detergent (1). Interestingly, the ompU strain did not display a competitive defect for colonization in the presence of the wild-type strain.In the present study the growth-phase-regulated outer membrane protein OpnS of X. nematophila was identified as a general porin that conferred a competitive advantage for growth in the insect host. OpnP and OpnS were the only general porins identified in the genome of X. nematophila. The reciprocal expression of OpnP and OpnS suggest that they serve distinct biological roles. 相似文献
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Toxin–antitoxin (TA) complexes play an important role in stress responses and programmed cell death in bacteria. The RelB-RelE toxin antitoxin system is well studied in Escherichia coli. In this study, we used combined in silico and in vitro approaches to study a novel Xn-RelT toxin from Xenorhabdus nematophila bearing its own antitoxin Xn-RelAT—a RelB homolog of E. coli. The structure for this toxin–antitoxin pair is yet unknown. We generated homology-based models of X. nematophila RelT toxin and antitoxin. The deduced models were further characterized for protein–nucleic acid, protein–protein interactions and gene ontology. A detrimental effect of recombinant Xn-RelT on host E. coli was determined through endogenous toxicity assay. When expressed from a isopropyl β-d-1-thiogalactopyranoside-regulated LacZ promoter, Xn-RelT toxin showed a toxic effect on E. coli cells. These observations imply that the conditional cooperativity governing the Xn-RelT TA operon in X. nematophila plays an important role in stress management and programmed cell death. 相似文献