共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
Transcriptional regulation of nanog by OCT4 and SOX2 总被引:39,自引:0,他引:39
Rodda DJ Chew JL Lim LH Loh YH Wang B Ng HH Robson P 《The Journal of biological chemistry》2005,280(26):24731-24737
5.
6.
7.
Granger A Bleux C Kottler ML Rhodes SJ Counis R Laverrière JN 《Molecular endocrinology (Baltimore, Md.)》2006,20(9):2093-2108
8.
9.
10.
11.
12.
13.
14.
15.
16.
Yamashita S Miyaki S Kato Y Yokoyama S Sato T Barrionuevo F Akiyama H Scherer G Takada S Asahara H 《The Journal of biological chemistry》2012,287(26):22206-22215
Sox9 plays a critical role in early chondrocyte initiation and promotion as well as repression of later maturation. Fellow Sox family members L-Sox5 and Sox6 also function as regulators of cartilage development by boosting Sox9 activation of chondrocyte-specific genes such as Col2a1 and Agc1; however, the regulatory mechanism and other target genes are largely unknown. MicroRNAs are a class of short, non-coding RNAs that act as negative regulators of gene expression by promoting target mRNA degradation and/or repressing translation. Analysis of genetically modified mice identified miR-140 as a cartilage-specific microRNA that could be a critical regulator of cartilage development and homeostasis. Recent findings suggest Sox9 promotes miR-140 expression, although the detailed mechanisms are not fully understood. In this study we demonstrate that the proximal upstream region of pri-miR-140 has chondrogenic promoter activity in vivo. We found an L-Sox5/Sox6/Sox9 (Sox trio) response element and detailed binding site in the promoter region. Furthermore, detailed analysis suggests the DNA binding and/or transactivation ability of Sox9 as a homodimer is boosted by L-Sox5 and Sox6. These findings provide new insight into cartilage-specific gene regulation by the Sox trio. 相似文献
17.
18.
19.