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1.
BackgroundThe levels of a number of essential and toxic trace elements in organs and tissues are affected by the disruptions in body homeostasis caused by obesity. Some of these elements may also be influenced by the consumption of biologically active substances of polyphenolic origin, which possess potent abilities to complex with transition metal ions.AimsThe aim of this study was to determine the content of essential and toxic trace elements in Wistar outbred and hereditary obese Zucker Leprfa (Z) rats consuming a standard balanced diet or hypercaloric diet with excess fat and fructose, supplemented with quercetin or not supplemented.Materials and methodsMale Wistar and Z rats were fed a control AIN-93M-based semi-synthetic diet or a high-fat-high-carbohydrate diet (HFCD, with 30% fat by weight and 20% fructose provided in the drinking water). A portion of the animals in each line and diet group was administered quercetin at 50 mg/kg body weight. Essential trace elements were included in the diets as a high-purity salt mixture. After the termination of feeding on day 63, the livers, kidneys, and brains of the rats were excised and the content of 16 elements (Fe, Mg, Cu, Mn, Co, Se, Zn, Cr, Ni, Al, Cd, As, Pb, V, Cs, and Ag) was measured by inductively coupled plasma mass spectrometry (ICP-MS).ResultsIn the livers of the Z rats, the contents of Co, Zn, Mg, Fe, Se, and V were reduced and the content of Cr was increased compared to that of the Wistar rats. Supplementation with quercetin significantly decreased liver Fe, V, and Se content, which was more noticeable in the Wistar rats than in the Z rats. In kidneys of Z rats consuming control diet, the contents of Co, Cu, and Cs were decreased whereas those of Ni, Al, and Se were increased compared with the contents in the Wistar rats. The same trend was observed with HFCD feeding except for Cs content. Quercetin reduced kidney V content in both rat lines fed both diets, whereas it reduced Se and Cs only in the Z rats fed control diet. In the brains of the Z rats, a large increase was observed in some trace elements including Pb, Cd, Al, Cr, Ni, Fe, and V compared with the levels in the Wistar rat brains. Supplementation of the control diet with quercetin decreased Al and Ni in the brains of the Z rats.ConclusionThere were significant differences in the mineral content of organs between the Wistar and Z rats, with different propensities for obesity. Moreover some of these effects had no straightforward association with decreased feed consumption or hepatic fat accumulation. When introduced into the diets, quercetin affected the content of essential and toxic elements, but with ambiguous physiological significance. Thus, indicators of essential and toxic trace elements deserve to be used in the protocols of preclinical as well as clinical trials of biologically active substances and food supplements. 相似文献
2.
Wojciech M. Kwiatek Tomasz Drewniak Mariusz Gajda Marek Gaka Albert L. Hanson Tadeusz Cichocki 《Journal of trace elements in medicine and biology》2002,16(3):155-160
In this study special interest was given to trace elements recognized as to be carcinogenic to humans. The kidney tissue sections were analyzed in order to determine the concentrations of elements present in the sample. The Synchrotron Radiation Induced X-ray Emission (SRIXE) technique was applied using a white photon microbeam. The results from cancerous parts of the kidney tissues were compared to non-cancerous parts and to the control group. In addition the iron concentration level was determined in the serum of those patients. Two-dimensional scans are presented to illustrate the differences between perfused and not-perfused tissues. According to this study there is no significant difference in the Mn concentration between cancerous and non-cancerous parts of the kidney, but the concentrations of Cd, Cr, Ti, V, Cu, Se, and Zn are at a lower concentration level in the cancerous parts than in the non-cancerous parts. A converse observation has been made for Fe. This may be associated with different metabolism and dynamics of the cancer process and both higher vascularization and need of higher blood supply in the cancerous tissue. The two-dimensional scanning of thin kidney sections showed differences in the trace element distributions depending on the analyzed samples: perfused and non-perfused. Perfusion removed blood mostly from the peritubular capillaries while in the glomerulus some capillaries had a relatively high Fe content. A low Fe concentration was observed in nephron tubules while a converse observation has been made for Cd. This may indicate that Cd is localized in the cells but not in the blood. 相似文献
3.
P. Irato G. C. Sturniolo G. Giacon A. Magro R. D’Inca C. Mestriner V. Albergoni 《Biological trace element research》1996,51(1):87-96
The present study was designed to investigate the effects of Zn administration on metallothionein concentrations in the liver, kidney, and intestine of copper-loaded rats. Male CD rats were fed a diet containing 12 mg Cu and 67 mg Zn/kg body wt. They were divided into either acute or chronic experimental protocols. Rats undergoing acute experiments received daily ip injections of either Cu (3 mg/kg body wt) or Zn (10 mg/kg body wt) for 3 d. Chronic experiments were carried out on rats receiving Cu ip injections on d 1, 2, 3, 10, 17, and 24, Cu injections plus a Zn-supplemented diet containing 5 g Zn/kg solid diet, or a Zn-supplemented diet alone. Rats injected Zn or Cu had increased MT concentrations in liver and kidney. Zn produced the most important effects and the liver was the most responsive organ. Rats fed a Zn-supplemented diet had significantly higher MT concentrations in liver and intestine with respect to controls. Increased MT synthesis in the liver may contribute to copper detoxification; the hypothesis of copper entrapment in enterocytes cannot be confirmed. 相似文献
4.
Reevaluating the free-ion activity model of trace metal availability to higher plants 总被引:5,自引:3,他引:5
The plant uptake and toxicity of many metals show a marked dependence on the aqueous speciation of the metal, and these responses often correlate best with the activity of the free metal ion. Exceptions to this generalization have been observed, however, and we sought to critically reexamine the theoretical foundation of the free-ion activity model (FIAM) of metal bioavailability to higher plants. Binding by an apoplastic functional group is often envisioned as a requisite step in the absorption or toxicity of a metal, and can be modeled in a variety of ways. Typically, however, speciation of the bulk solution is calculated without regard to such surface binding, even though it could influence the pertinent mass balance expressions. A more thorough treatment considers simultaneous formation of both the metal-ligand complex in solution (ML) and the metal-cell surface complex (M-X). Here, empirical conformity to the FIAM can be expected, but only under pivotal assumptions about the relative sizes of the test solution and the root biomass, and about the relative binding strength of L and -X. Moreover, empirical conformity to the FIAM does not preclude cell-surface binding of the complexed metal followed by ligand exchange (ML + -X M-X + L), so that correlations between biological response and free metal-ion activity imply nothing about the molecular species that actually interacts with the cell surface. Computer simulations of Cu (II) binding by a model apoplastic ligand are used to illustrate these and other key features of the FIAM. Departures from the FIAM seem most likely when (i) the quantity of the metal-complexing ligand is limited (as may be the case in soil solution or in the rhizosphere), and/or (ii) the solution ligand is very weak. 相似文献
5.
Ciftci TU Ciftci B Yis O Guney Y Bilgihan A Ogretensoy M 《Biological trace element research》2003,95(1):65-71
The effectiveness and success of antituberculosis therapy is mainly measured by its ability to identify the organism in the
sputum. In certain cases, available tuberculosis tests are not satisfactory and do not provide enough information on the effectiveness
of antituberculosis therapy. Copper (Cu), zinc (Zn), and selenium (Se) are the essential elements that play a crucial role
in the immune system. The serum levels of these elements vary in many diseases including tuberculosis. In this study, we investigate
whether the serum levels of Cu, Zn, and Se change during antituberculosis therapy. We have included 22 pulmonary tuberculosis
cases that were newly diagnosed with positive sputum and 18 healthy subjects. At the beginning and 2 mo after therapy, serum
levels of Cu, Zn, and Se were measured by atomic absorption spectrometry. Despite Se and Cu levels not being affected during
the treatment, we found that there was a significant increase in the levels of Zn and a decrease in the Cu/Zn ratio. Serum
Zn levels and the Cu/Zn ratio could be used as a valuable laboratory tool for the clinicians to assess response to therapy
or effectiveness of the ongoing antituberculosis therapy. 相似文献
6.
Tatari H Işlekel H Altekin E Göçen S Ozcan C Ergör A 《Biological trace element research》2001,80(1):33-42
Changes in serum magnesium, copper and zinc values were evaluated in spinal fusion patients at four monitorings. For magnesium
and copper individually, a significant difference was found between the mean values at each monitoring (p<0.05), whereas the changes of zinc values between four monitorings were insignificant. There was no statistical difference
between the changes of each trace element values and gender, age, operative time, intraoperative blood loss, blood replacement,
number of the vertebral levels fused, and antibiotic type used.
As a result, magnesium can be suggested to be more important than the other two elements in the postoperative period. Alterations
of serum magnesium, copper, and zinc values do not have any correlation with the fusion of the spinal column, either with
the width of the fused area or operative time and blood loss. This study cannot confirm the exact reason for this entity and
the etiology remains speculative. There is no need for magnesium, copper, or zinc supplementation during the surgical period
for the patients. It will be worthy to evaluate the patients who were sent to the intensive care unit after spinal surgery
and compare their results with the other intensive care patients. 相似文献
7.
Trace elements are known to have a key role in myocardial metabolism. The accumulation (cobalt, arsenic, copper) or deficiency
(selenium, zinc) of trace elements may be responsible for idiopathic dilated cardiomyopathy. We investigated the trace element
concentrations (Cu, Zn, Mg) in sera from patients with dilated cardiomyopathy by atomic absorption spectrophotometry. We observed
that patients with dilated cardiomyopathies have higher copper and lower zinc concentrations in serum than healthy controls.
The magnesium concentrations of patients did not differ significantly from that of control subjects. 相似文献
8.
Mahomed K Williams MA Woelk GB Mudzamiri S Madzime S King IB Bankson DD 《Biological trace element research》2000,75(1-3):107-118
Preeclampsia is an important cause of maternal and perinatal mortality worldwide. The etiology of this relatively common medical
complication of pregnancy, however, remains unknown. We studied the relationship between maternal leukocyte selenium, zinc,
and copper concentrations and the risk of preeclampsia in a large hospital-based case-control study. One hundred seventy-one
women with proteinuric pregnancy-induced hypertension (with or without seizures) comprised the case group. Controls were 184
normotensive pregnant women. Leukocytes were separated from blood samples collected during the patients’ postpartum labor
and delivery admission. Leukocyte concentrations for the three cations were measured by inductively coupled plasma-mass spectrometry
(ICP-MS). Concentrations for each cation were reported as micrograms per gram of total protein. Women with preeclampsia had
significantly higher median leukocyte selenium concentrations than normotensive controls (3.23 vs 2.80 μg/g total protein,
p<0.0001). Median leukocyte zinc concentrations were 31% higher in preeclamptics as compared with controls (179.15 vs 136.44
μg/g total protein, p<0.0001). Although median leukocyte copper concentrations were slightly higher for cases than controls, this difference did
not reach statistical significance (17.72 vs 17.00 μg/g total protein, p=0.468). There was evidence of a linear increase in risk of preeclampsia with increasing concentrations of selenium and zinc.
The relative risk for preeclampsia was 3.38 (adjusted odds ratio [OR]=3.38, 95% confidence interval [CI]=1.53–7.54) among
women in the highest quartile of the control selenium distribution compared with women in the lowest quartile. The corresponding
relative risk and 95% CI for preeclampsia was 5.30 (2.45–11.44) for women in the highest quartile of the control zinc distribution
compared with women in the lowest quartile. There was no clear pattern of a linear trend in risk with increasing concentration
of leukocyte copper concentrations (adjusted for linear trend in risk =0.299). Our results are consistent with some previous
reports. Prospective studies are needed to determine whether observed alterations in selenium and zinc concentrations precede
preeclampsia or whether the differences may be attributed to preeclampsia-related alterations in maternal and fetal-placental
trace metal metabolism. 相似文献
9.
微量金属参与了生物体许多化学反应过程,同时也可作为蛋白质的辅基或辅因子起作用,对机体生长发育以及正常生物功能的维持具有重要作用;微量金属元素的代谢失衡与生物体许多疾病密切相关,如威尔森氏病、门克斯病、铁色素沉积、肠变性皮炎以及一些神经退行性疾病。黑腹果蝇(Drosophila melanogaster)是遗传背景清楚、生活周期短、操作方便的模式生物,利用果蝇研究金属离子代谢以及金属离子代谢与疾病的联系具有独特的优势,近年来,随着果蝇基因组测序的完成以及许多转基因果蝇株的建立,果蝇也越来越多的用于金属离子代谢的研究。介绍了近年来果蝇在金属离子代谢研究领域的进展,以及其与神经退行性疾病关系研究上的一些应用。 相似文献
10.
微量元素如铁、锌、铜等对维持生物体代谢和健康至关重要,其含量失衡会造成代谢异常甚至死亡,因此生物体存在复杂机制维持这些微量元素的稳态代谢平衡(homeostasis)。近年来国际上一些实验室尝试用模式脊椎生物斑马鱼来开展该领域的研究,展示出斑马鱼的特有优势。特别是大规模正向遗传学筛选的成功开展,一系列微量元素代谢异常的突变体(如:weissherbst、chardonnay、chianti、shiraz、gavi、calamity和catastrophe)相继发现,为研究离子代谢调控机制和相关疾病的发病机理,提供了整体动态的活体模型。铁代谢相关基因加,2J和grx5都己在斑马鱼中成功定位克隆,斑马鱼铜载体基因atp7a突变体calamity的深入研究,进一步阐明了Menkes病的发病机理。利用斑马鱼的优势,结合小鼠模型和人群来研究微量元素的体内稳态代谢平衡将是微量元素代谢机制研究的新方向。 相似文献
11.
The effectiveness and success of antitubercular therapy is mainly measured by identifying the organism in sputum. In certain
patients, especially in geriatric patients, available tuberculosis tests are not satisfactory and do not provide enough information
on the effectiveness of antitubercular therapy, as the symptoms might be confused with the existing symptoms of ongoing diseases.
Therefore, 60 diagnosed and randomly selected patients with tuberculosis were included into this study. The patients with
other associated diseases likely to influence serum copper and zinc were not included in the study. The estimations of serum
copper and zinc were done in healthy volunteers and in tubercular patients before the start of treatment and after 4 wk of
antitubercular treatment. The average plasma concentration of serum copper and zinc in healthy volunteers were 102±20 μg/dL
and 96±18 μg/dL respectively. In tuberculosis patients, serum copper and zinc levels were 123.65±9.98 μg/dL and 64.14±3.97
μg/dL, respectively, before the start of treatment, which came down to 116.23±4.27 μg/dL and 74.31±3.60 μg/dL, respectively,
after 4 wk of antitubercular treatment. 相似文献
12.
Gerrit J. Van den Berg Shiguang Yu Annet Van der Heijden Arnoldina G. Lemmens Anton C. Beynen 《Biological trace element research》1993,38(2):107-115
The hypothesis was tested that dietary fructose vs glucose lowers copper solubility in the digesta in the small intestine
of rats, which in turn causes a decreased copper absorption. Male rats were fed adequate-copper (5 mg Cu/kg) diets containing
either fructose or glucose (709.4 g monosaccharide/kg) for a period of 5 wk. Fructose vs glucose significantly lowered copper
concentrations in plasma and the liver, but did not alter hepatic copper mass. Fructose feeding resulted in a significantly
lesser intestinal solubility of copper as based on either a smaller soluble fraction of copper in the liquid phase of small
intestinal contents or a lower copper concentration in the liquid phase. The latter fructose effect can be explained by the
observed fructose-induced increase in volume of liquid phase of intestinal digesta. After administration of a restricted amount
of diet extrinsically labeled with64Cu, rats fed fructose also had significantly lower soluble64Cu fraction in the digesta of the small intestine. Although this study shows that fructose lowered intestinal copper solubility,
only a slight reduction of apparent copper absorption was observed. It is suggested that the fructose-induced lowering of
copper status in part counteracted the fructose effect on copper absorption at the level of the intestinal lumen. 相似文献
13.
Sriwatana Songchitsomboon Surat Komindr Noppawam Plaseu 《Biological trace element research》1998,61(1):97-104
The levels of intake and food sources of zinc and copper in 231 20–80 yr old healthy adults living in Bangkok and surrounding districts were determined. Dietary data from 3-d food records collected and validated at Research Center, Ramathibodi Hospital were analyzed. Levels of zinc and copper intake were compared between sexes and age groups (20–39, 40–59, and 60–80 yr). Mean daily zinc and copper intakes (±SEM) in men were 6.3 ±0.2 and 1.9 ±0.1 mg, respectively. Mean daily zinc and copper intakes (±SEM) estimated in women (5.5 ±0.2 and 1.6 +-0.1 mg) were significantly lower. Higher zinc and copper intakes were found in the younger (20-39 yr) age group of both sexes. This could be explained by higher density and percentage from animal source of both nutrients. Consumption of various types of meat, fish, egg, and milk accounted for 42 and 22%, and rice, the staple food of Thai people for 9 and 23% of total dietary zinc and copper, respectively. 相似文献
14.
Zachara BA Włodarczyk Z Masztalerz M Adamowicz A Gromadzinska J Wasowicz W 《Biological trace element research》2004,97(1):1-13
In animals and humans, the highest level of selenium (Se) occurs in the kidney. This organ is also the major site of the synthesis
of the selenoenzyme glutathione peroxidase (GSH-Px). Decreased Se levels and GSH-Px activities in blood are common symptoms
in the advanced stage of chronic renal failure (CRF). Blood samples for Se levels and GSH-Px activities measurements from
patients were collected just before transplantation and 3, 7, 14, 30, and 90 d posttransplant. The Se levels in whole blood
and plasma of patients before transplantation (79.5 and 64.5 ng/mL, respectively) were lower by 23% and 21%, respectively,
as compared with controls (p<0.0001), and 7 d after operation, it further decreased in both components (p<0.01). Fourteen days after surgery, the levels reached the initial values and increased slowly in the later period. Red blood
cell GSH-Px activity in patients in the entire period of the study did not differ from the control group. Plasma GSH-Px of
patients before the surgery was extremely low (76 U/L) as compared with controls (243 U/L; p<0.0001) but increased rapidly to 115 U/L after 3 d, to 164 U/L after 14 d, and to 208 U/L after 3 mo posttransplant. In CRF
patients, after kidney transplantation, plasma GSH-Px activity increased rapidly, approaching, after 3 mo, the values that
were close to the normal levels. A negative correlation between creatinine level and plasma GSH-Px activity is observed in
patients after kidney transplantation. Monitoring of plasma GSH-Px activity may be a useful additional marker of the transplanted
kidney function. 相似文献
15.
Mineral and trace element interactions were studied in a balance trial with rats. Calcium, copper, and zinc were supplied
to a rapeseed meal diet in a factorial design. Animals were fedad libitum, and absorption, excretion, and retention of the elements were evaluated either as fractions of total intake or in relation
to nitrogen retention to account for differences in food intake and lean body mass increment. The intrinsic content of minerals
and trace elements was sufficient to support growth at a rate that could be expected from the rapeseed protein quality. However,
when calcium was included in the diet, the intrinsic dietary level of zinc appeared to be limiting, despite the fact that
the zinc level was twice the recommended level. Additional zinc supply reversed growth impairment. This calcium-zinc interaction
is believed to be owing to the formation of phytate complexes. Calcium addition influenced the calcium, phosphorus, magnesium,
zinc, and iron—but not the copper—balances. The addition of calcium reduced the availability of the intrinsic zinc, whereas
no effect was seen in the zinc-fortified groups. The availability of intrinsic copper was in a similar way significantly impaired
by addition of dietary zinc, where-as copper-supplied groups were unaffected by zinc addition. Intrinsic iron availability
was also dependent upon zinc addition, although in a more ambigouus way. Thus, addition of extrinsic minerals to a diet high
in phytate can result in significant impairments of growth and mineral utilization. 相似文献
16.
Elzbieta Gle-Haduch Krzysztof Szostek Henryk Gb 《American journal of physical anthropology》1997,103(2):201-207
Determination of element levels in bones and teeth can complement knowledge of the diagnostics and etiology of various diseases in prehistoric populations. Calcium (Ca), cadmium (Cd), copper (Cu), iron (Fe), and lead (Pb) content were analyzed in teeth from human skeletons dated to 3,000–1,400 BC from Malopolska Upland loess. Levels of iron and calcium were determined using atomic absorption spectroscopy (AAS), and lead, cadmium, and copper levels were measured using anodic stripping voltametry (ASV). Molar teeth from specimens with cribra orbitalia were selected for analyses, and teeth from specimens with no pathological changes were used as a control. No significant correlations between the content of particular elements and the tooth class, specimen age, or depth of burial pit were observed. The Fe content in specimens with cribra orbitalia is not the best measure for this disease's etiology. Thus, interelement correlations and proportions might give a better picture of the biological condition of the specimen and of the investigated groups. Am J Phys Anthropol 103:201–207, 1997. © 1997 Wiley-Liss, Inc. 相似文献
17.
Forrest H. Nielsen Thomas J. Zimmerman Terrence R. Shuler 《Biological trace element research》1982,4(2-3):125-143
In two fully crossed, three-way, two by three by three, factorially arranged experiments, female weanling rats were fed a
basal diet supplemented with iron at 15 and 45 μg/g, nickel at 0, 5, and 50 μg/g and copper at 0, 0.5, and 5 μg/g (Expt. 1)
or 0, 0.25, and 12 μg/g (Expt. 2). Expt. 1 was terminated at 11 weeks, and Expt. 2 at 8 weeks because, at those times, some
rats fed no supplemental copper and the high level of nickel began to lose weight, or die from heart rupture. The experiments
showed that nickel interacted with copper and this interaction was influenced by dietary iron. If copper deficiency was neither
very severe or mild, copper deficiency signs of elevated levels of total lipids and lipid phosphorus in liver and plasma,
and cholesterol in plasma, were made more severe by supplemental dietary nickel. Rats in which nickel supplementation exacerbated
copper deficiency did not exhibit a depressed level of copper in liver and plasma. Also, although iron deprivation enhanced
the interaction between nickel and copper, iron deprivation did not significantly depress the level of copper in liver and
plasma. The findings confirmed that, in rats, a complex relationship exists between nickel, copper, and iron, thus indicating
that both the iron and copper status of experimental animals must be controlled before data about nickel nutriture and metabolism
can be compared among studies. 相似文献
18.
Karul AB Karadag F Yensel N Altinisik M Altun C Cildag O 《Biological trace element research》2003,94(1):41-47
We searched for serum concentrations of trace elements and correlated them to malondialdehyde (MDA), which is an indirect
marker of oxidative stress, in order to clarify if routine evaluation is necessary in chronic obstructive pulmonary disease
(COPD) outpatients. Serum concentrations of copper (Cu), zinc (Zn), and magnesium (Mg) were determined by atomic absorption
spectrophotometry and iron (Fe) by a ILLab 1800 autoanalyzer with ILLab test kits. Serum MDA concentrations were detected
in terms of TBARS (thiobarbituric acid reactive substances) spectrophotometrically.
Serum Cu, Zn, Mg, Fe, and MDA concentrations in patient and control groups were all in the normal reference range. The results
respectively were as follows: Cu:123±29.2 and 122.2±23.4 μg/dL; Zn: 87.8±17.8 and 96.9 ± 12.9 μg/dL; Mg: 2.3±0,5 and 2.04±0.28
mg/dL; Fe: 73.8±35.5 and 80.7±51.2 μg/dL; MDA: 1.09±0.11 and 0.95±0.06 nmol/L. MDA was not correlated to Cu, Zn, Mg, or Fe
(p>0.05 for all). The serum Zn concentration of COPD group was lower than the control group (p=0.042), whereas the Mg concentration was higher (p=0.021). There was no statistical difference in other study parameters. Oxidative stress was not increased in clinically stable,
regularly treated COPD patients. Although there was no deficiency in trace elements (Cu, Fe, Mg, and Zn), serum Zn was close
to the lower limit of the reference value. There is no need for routine evaluation of trace elements in clinically stable,
regularly treated COPD outpatients. 相似文献
19.
Variation in the distribution of trace elements in hepatoma 总被引:3,自引:0,他引:3
There are many reports of reduction of zinc level and rise of copper level in serum of patients with liver disease. However,
there are a few reports that compare the trace elements in tumor tissues and nontumor tissues of the liver with hepatoma.
We studied trace element distribution in tumor tissues and nontumor tissues of liver with hepatoma and compared them with
data from normal liver tissues. Zinc (Zn), copper (Cu), selenium (Se), cadmium (Cd), mercury (Hg), and iron (Fe) were chosen
as the trace elements to be observed.
We observed falls of Zn, Cd, and Hg levels in tumor tissues and the rise of Cu level as a result of this investigation. Zn,
Cd, and Hg levels in tumor tissues were significantly lower than those in nontumor tissues and Zn, Cd, and Hg levels in nontumor
tissues were significantly lower than in normal liver tissues. This tendency was clearer for Cd and Hg than for Zn. Although
the distribution of Cu was not significant, a distribution contrary to that of Zn was shown.
These findings indicate that the distribution of Zn, Cd, and Hg can serve as supportive evidence that could be useful as a
tumor marker. Selenium showed almost the same accumulation tendency among tumor tissues, nontumor tissues, and normal livers.
Although correlation was observed among most metals in the normal liver, there was almost no correlation in tumor tissues. 相似文献
20.
大量研究表明,高果糖可引起脂肪肝,但对肾脏脂质代谢的影响尚不清楚。该实验研究给予10%果糖水5周后诱导的脂肪肝大鼠肾脏的脂质代谢情况,并探讨其可能机制。将16只雄性SD大鼠随机分为正常组(con)和果糖组(fru),果糖组给予10%(W/V)果糖水,第5N末称体重、取血、处死,检测血浆GLU、TG、TC和INSULIN含量。取肾脏、肝脏和白色脂肪称重,采用形态学方法观察肝脏和肾脏脂质沉积情况,酶法测其TG、TC含量,以Real time—PCR检测肾脏、肝脏中脂质合成和脂质氧化相关基因水平,以Westemblot检测肾、肝细胞核脂质合成转录因子的蛋白表达。结果显示,果糖组大鼠血浆TG、INSULIN明显升高,并出现肥胖体征,肝脏脂质沉积严重,其调控脂质合成的两个关键的转录因子ChREBP和SREBPlcmRNA和核蛋白表达都明显升高,并且它们靶向的脂质合成相关酶FAS、ACCl、SCDlmRNA表达也显著增加。但是,在肾脏中,高果糖没有引起TG含量的变化,调控脂质重新合成的基因和蛋白的表达也未发生变化。因此,与果糖致脂肪肝不同,高果糖饮食并没有造成肾脏的脂质沉积和脂质合成相关基因、蛋白的变化。 相似文献