共查询到20条相似文献,搜索用时 62 毫秒
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Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids. 总被引:17,自引:0,他引:17 下载免费PDF全文
N Hosokawa K Hirayoshi H Kudo H Takechi A Aoike K Kawai K Nagata 《Molecular and cellular biology》1992,12(8):3490-3498
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(E)-9-(1-pyrenyl)-4-hydroxynon-2-enal (FHNE), a fluorescent probe of (E)-4-hydroxynon-2-enal (HNE) is synthesised in seven steps and in 35% overall yield, starting from commercially available 1-pyrencarboxyaldehyde. When incubated with cultured HeLa cells this fluorescent probe penetrates cells and particularly concentrates in the region surrounding the nucleus. As the parent compound, HNE it is able to induce the activation of heat shock factor (HSF) and it is able to induce the binding of HSF to heat shock element (HSE). 相似文献
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The DNA-binding activity of a tobacco heat shock factor (HSF) was induced by heat treatment (37–40 °C) of a cell-free extract that contained extra-nuclear fraction, but not in an extract of isolated nuclei. These observations suggest that an inactive form of HSF can directly recognize and transduce the heat shock signal and that such transduction requires components of the extranuclear fraction. Addition of ATP or of most other nucleoside triphosphates reduced the binding of the HSF to the heat shock element (HSE) in the same extract, and removal of ATP by dialysis from the extract restored the ability of the HSF to bind to DNA. The restored activity of the HSF could be eliminated again by a second addition of ATP. Our observations provide the first example of the involvement of ATP in the regulation of the reversible changes in HSF that control its ability to bind to HSEs in a cell-free extract.Abbreviations AMP-PNP
adenylyl imidodiphosphate
- GUS
-glucuronidase
- HSE
heat shock element
- HSF
heat shock factor 相似文献
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Mathew A Mathur SK Jolly C Fox SG Kim S Morimoto RI 《Molecular and cellular biology》2001,21(21):7163-7171
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(E)-9-(1-pyrenyl)-4-hydroxynon-2-enal (FHNE), a fluorescent probe of (E)-4-hydroxynon-2-enal (HNE) is synthesised in seven steps and in 35% overall yield, starting from commercially available 1-pyrencarboxyaldehyde. When incubated with cultured HeLa cells this fluorescent probe penetrates cells and particularly concentrates in the region surrounding the nucleus. As the parent compound, HNE it is able to induce the activation of heat shock factor (HSF) and it is able to induce the binding of HSF to heat shock element (HSE). 相似文献
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Interaction of the DNA-binding domain of Drosophila heat shock factor with its cognate DNA site: a thermodynamic analysis using analytical ultracentrifugation. 总被引:3,自引:0,他引:3 下载免费PDF全文
S. J. Kim T. Tsukiyama M. S. Lewis C. Wu 《Protein science : a publication of the Protein Society》1994,3(7):1040-1051