共查询到20条相似文献,搜索用时 187 毫秒
1.
Adsorption of fungal spores on a synthetic polymer - High Density Polyethylene was successfully achieved using different pretreatments. Sonication of beads followed by ferric nitrate treatment or use of 0.1% tributyrin gave adsorption upto 46%. Use of dichloromethane as a solvent for sonication is recommended for its better performance in reuse studies (upto 5 times without much decrease in activity). 100 g of immobilized biocatabyst in a 7 L Fluidized Bed Bioreactor was found to perform better than shaker flask at a much lower power input. 相似文献
2.
Deepa Geeta Devi Yadav Pooja Chaudhary Mohd Jubair Aalam Dhan Raj Meena Surendra Singh 《Chirality》2020,32(1):64-72
Imidazolidin-4-one is used as a recoverable organocatalyst for the asymmetric Diels-Alder reaction in the presence of catalytic amount of dicationic ionic liquid and trifluoroacetic acid as a co-catalyst. The Diels-Alder reaction between model substrate cyclopentadiene and crotonaldehyde gave the product in 95% conversion and 87% ee of the endo-product. The catalyst was shown better reusability when the 20 mol% of dicationic ionic liquid was used and catalyst was reused upto 5 cycles, conversion remains upto 3 recycles but ee of endo- 9 was slightly droped. 相似文献
3.
The survival of certain pathogenic bacteria was studied in anaerobic batch digesters at room temperature (18–25 °C) as well as at 35 °C under laboratory conditions. The survival of Escherichia coli and Salmonella typhi at room temperature was upto 20 days whereas at 35 °C it was only upto 10 days. Shigella dysenteriae was found to be the most sensitive organism which could survive upto 10 days at room temperature and upto 5 days at 35 °C. The longest survival was observed in case of Streptococcus faecalis which could survive upto 35 days at room temperature and 15 days at 35 °C. The survival time of Salmonella typhi increased when the solid contents of the digester were elevated from 9% to 15%. 相似文献
4.
Om Prakash Lata Shea Bachan Upadhyay 《Journal of plant biochemistry and biotechnology.》2005,14(2):209-213
Watermelon (Citrullus vulgaris) urease was immobilized in 3.5% alginate leading to 72% immobilization. There was no leaching of the enzyme over a period of 15 days at 4°C. It continued to hydrolyse urea at a faster rate upto 90 min of incubation. The immobilized urease exhibited a shift of apparent pH optimum by one unit towards acidic side (from pH 8.0 to 7.0). The Km was found to be 13.3 mM; 1.17 times higher than the soluble enzyme (11.4 mM). The beads were fairly stable upto 50°C and exhibited activity even at ?10°C. The enzyme was significantly activated by ME and it exhibited two peaks of activation; one at lower concentration and another at higher concentration. Time-dependent ureolysis in presence of ME progressed at a much elevated rate. Unlike soluble enzyme, which was inhibited at 200 mM urea, the immobilized enzyme was inhibited at 600 mM of urea and above, and about 47% activity was retained at 2000 mM urea. Moreover, the inhibition caused by high urea concentration was partially abolished by ME. The significance of the observations is discussed. 相似文献
5.
Summary Through Mo fertilization and N or P or NP enrichment, cultures of inoculant algaT. tenuis were prepared in situ in a rice-growing clay loam. The pattern of distribution of total, organic and mineral N was studied
during the physiological stages of tillering, inflorescence and harvest stages of rice with particular reference to Mo-fertilized
soils. Mineralisation sequence was studied in the postharvest stage also upto 250 days including the life span of the rice
plant.
Though effect of fertilizer was generally more prominent than that of algalization, the latter in Mo-fertilized soils, could
signify substantially higher mineral N, and also the low mineralisable index of N, a phenomenon very much desirable for slow
release of soil reserve. A typical curve of soil N pattern possesses maximal level at tillering with levelling off at the
harvest stage, but the curve of soil N pattern under algalization in Mo-fertilized soils was a hyperbolic one, having a maximal
value at tillering.
There was no substantial N addition in control soils with or without alga. Algalization became effective only in Mo-fertilized
soils upto 24–29% at tillering and 40% at blooming. Algalization with P or NP application in Mo-fertilized soils caused a
50% rise in the organic N. Sustenance of levels of both total and organic N beyond the tillering stage was a characteristic
feature of algal inoculation. 相似文献
6.
Red light stimulated calcium uptake in maize leaf protoplasts upto 140% over the dark control. The stimulation was maximally noticed after 120 seconds of incubation and was reversed by far red light. 5-Hydroxytryptamine also enhanced calcium uptake in dark, upto the red level. A possible role of phosphoinositide for signal transduction in calcium uptake for phytochrome mediated responses in higher plants is suggested. 相似文献
7.
N-Methyl-alpha,alpha-diphenyl-L-prolinol derivatives with para-bromo substituents in one or both of the phenyl rings are easily bound to crosslinked polystyrene beads containing phenylboronic acid residues using Suzuki reactions. When the products were used as catalysts for the reactions of aldehydes with diethylzinc in toluene at 20 degrees C, the alcohols were produced in chemical yields >90% and with ees of upto 94%. The better of the two supported catalysts gave ees only 0-9% lower than those obtained with the corresponding soluble catalyst. One of the supported catalysts was recycled successfully nine times. 相似文献
8.
Summary The effect of CaCO3 and iron on the availability of iron, manganese phosphorus and calcium was studied in the greenhouse on pea (Pisum sativum L.) crop on a light textured soil, which was marginal in exchangeable calcium. Addition of calcium carbonate caused significant increase in dry matter yield with no added iron at both the stages of crop growth. But yeild intended to decrease with 8% CaCO3 at 75 days of crop growth. Dry matter yield also increased with the addition of iron upto 10 ppm at 45 days and upto 5 ppm at 75 days. The iron concentration and uptake decreased with the increase in CaCO3 and increased with the application of iron at both the stages of crop growth. The application of iron and CaCO3 decreased concentration and uptake of phosphorus significantly at both the stages.Like phosphorus, concentration and uptake of manganese also decreased with the increase in added CaCO3 upto 8% and iron upto 20 ppm at 45 and 75 days. The concentration of calcium increased with the addition of CaCO3 to the extent of 50 and 40% with 8% CaCO3 at 45 and 75 days, while the uptake of calcium increased more than 3 folds at 45 days and more than 2 folds at 75 days. The concentration of calcium decreased with the application of iron upto 20 ppm but the uptake at 45 days increased upto 10 ppm and at 75 days upto 5 ppm and then decreased.The concentration of Fe, P and Ca decreased at 75 days and that of Mn increased while the uptake of all these nutrients increased at 2nd stage due to higher dry matter. 相似文献
9.
Various ion-dependent (Na+K+, Ca++ and Mg++) ATPases activities in liver cell nuclear membrane have been determined after a single injection of different doses (0.01, 0.025, 0.05, 0.1, 0.25, 0.5, 1, 2 and 4 micrograms/g) of L-triiodothyronine (T3) in Singi fish, Heteropneustes fossilis Bloch. Administration of T3 at a minimum effective dose of 0.05 micrograms upto 4 micrograms/g induced a rise (14 to 43% over control value) in the Na+K+-ATPase activity in a dose-dependent fashion maximum upto 1 microgram/g dose, whereas Ca++-ATPase showed a dose-dependent increase (20 to 43% over control) with 0.25-1 microgram/g of T3, although the increase in the respective enzyme activity was maintained upto 4 micrograms/g of T3 dose. Mg++-ATPase activity in liver cell nuclear membrane was found to be increased at 1 microgram-4 micrograms/g of T3 dose, showing a similar magnitude of increase (7% over the control value) with these doses of T3. Other doses of T3 (0.01 and 0.025 micrograms/g) were ineffective in altering the different ion-specific ATPase activity. Treatment of Singi fish with thiourea (1 mg/ml) for 30 days caused a significant fall in Na+K+, Ca++ and Mg++-ATPase activities upto 21%, 17% and 5%, respectively, below the euthyroid control level. A single injection of T3 at the dose of 1 microgram/g in the hypothyroid fish raised the Na+K+ and Ca++-ATPase activities to about 36% over the control value, and the Mg++-ATPase activity was restored to only the control level. Thus a dose-dependent nuclear effect of T3 is evident from the present investigation. 相似文献
10.
Summary A green-house experiment was conducted to study P–Cu relationship in wheat variety S-308 grown up to maturity. Treatments included five levels, each of P (0, 25, 50, 100 and 250 ppm) and Cu (0, 2.5, 5, 10 and 50 ppm) in all possible combinations. The yield, P and Cu concentration were more with the application of Cu upto 5 ppm with P, and P upto 50 ppm with Cu, over P alone and Cu alone, respectively. Application of 50 ppm Cu adversely affected yield and P concentration and 250 ppm P adversely affected yield and Cu concentration. Application of affected nutrient counteracted to a great extent the adverse effect of the other nutrient. The low levels of P and Cu were essential for better plant growth and efficient utilization of both the nutrients and antagonism was noted only when one of the nutrients was applied in high quantities.Contribution from the Department of Soils, Haryana Agric. Univ., Hissar-India. 相似文献
11.
The plants of sesame white (Sesamum indicum L. var. T55) grown on tannery sludge (TS) contaminated soil have shown that Cr level in the seeds was found below detection limits in 10% and 25% TS, however, the levels of Ni, Pb and Cd were found above the recommended limits. In roots, the level of antioxidants increased in the plants grown upto 35% TS at 30d over their respective controls. Total chlorophyll content increased significantly (p<0.5) in the plants (leaves) grown on lower sludge amendments (upto 35% TS at 30d and 25% TS at 60d) over their respective controls. In addition, the oil content increased (35% increase over control) in the plants grown on 35% TS. No significant change was observed in thiobarbituric acid reactive substances (TBARS), a lipid peroxidation index, in the plants (upto 50% TS). The number of trichomes in the leaves of treated plants was found more than control. In lower and upper leaves surfaces, the anterior end of the trichomes was found acute tipped and bent downwards, whereas, the trichome tip was straight and blunt in control. The stomata on upper and lower surfaces of the leaves were found partially or totally closed in the plants grown on 100% TS as compared to control. The toxicity was observed at higher amendments which are evident from the observed morphological changes and decrease in chlorophyll content. This study concludes that it is not advisable to grow the plants on contaminated area, besides its healthy growth. 相似文献
12.
Diazotrophic heterocystous cyanobacteria Nostoc calcicola and Anabaena sp. ARM 629 were investigated for their ability to grow in presence of sodium bicarbonate (NaHCO3) or carbon dioxide (CO2) under cultural conditions. Maximum growth was observed in 75 mM NaHCO3 and 5% CO2 in N. calcicola and Anabaena ARM 629, respectively. Although their growth rate declined, N. calcicola and Anabaena sp. could tolerate upto 250 mM NaHCO3 and 20% CO2, respectively. N-methyl-N'-nitro N nitrosoguanidine induced mutants of these cyanobacteria were isolated which showed growth upto 1 M NaHCO3 (N. calcicola) or 50% CO2 (Anabaena sp.) in comparison to their wild types. The mutants also showed cross-resistance to either of the inorganic carbon compounds, which was not observed for wild type. It was concluded that mutants were altered in multiple properties enabling them to grow at elevated levels of inorganic carbon compounds. 相似文献
13.
Influence of pre-sowing chilling treatments to seeds on seed germination and accumulation of osmotics in Indian mustard [Brassica
juncea (L.) Czernj & Cosson] under NaCl stress was investigated. Germination was 100 % in water soaked non-chilled (control)
seeds upto 100 mM NaCl, whereas it was upto 200 mM NaCl in chilling treated seeds. Pre-sowing chilling treatments to seeds
for 5, 10 and 15 d also enhanced the dry mass of 6-d-old seedlings, and concentrations of saccharides and amino acids. The
alleviation of NaCl stress by pre-sowing chilling treatments was also associated with decrease in Na+ and proline accumulation and a slight increase in K+ and Ca2+ contents of seedlings.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
14.
Summary Inoculation of water fernAzolla pinnata R. Brown (Bangkok isolate) at the rate of 500kg fresh weight ha−1 in rice fields at weekly intervals after planting in addition to 30 kg N ha−1 as urea showed a decrease in its growth and N2-fixation with delay in application. Use of Azolla up to 3 weeks after planting (WAP) during wet and 4 WAP during dry season
produced significantly more grain yield than 30 kg N ha−1, whereas its application upto one WAP produced more grain yield than 60 kg N ha−1. Grain yield with Azolla applied at the time of planting was similar to that of 60 kg N treatment during the wet season.
Higher grain yields in zero and one WAP Azolla treatments resulted due to increase in both number of panicles m−2 and number of grains/panicle while the subsequent Azolla inoculations increased grain yield mainly by producing more number
of grains/panicle.
Dry matter and total N yields at maturity of rice crop were more with Azolla application upto 3 WAP during wet and 2 WAP during
dry season while the reduction in sterility (%) was observed upto one WAP over 30 kg N ha−1 during both seasons. Number of tillers m−2 and dry matter production at maximum tillering and flowering were more than 30 kg N ha−1 with the use of Azolla upto one WAP. Increased grain N yield was observed with the use of Azolla upto 4 WAP during two seasons
whereas straw N yield increased upto one WAP during wet and 2 WAP during dry season. 相似文献
15.
Antioxidant potential of Aspergillus candidus MTCC 2202 broth filtrate extract was studied using different antioxidant models, whereas anti-inflammatory potential was studied using carrageenan-induced rat paw oedema model. The ethyl acetate extract at 1000 microg/ml showed maximum scavenging activity of the stable radical 1,1-diphenyl,2-picryl hydrazyl upto 96.65% (IC50=430.36 microg/ml) and scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) upto 92.25% (IC50=606.29 microg/ml) at the same concentration. The extract had good reducing power, however showed moderate inhibition for conjugated dienes and thiobarbituric acid reactive acid substances (59.56 and 51.45%). The total phenolic content of various extracts of A. candidus broth filtrate was measured and a correlation between radical scavenging activities of extracts with total phenolic content was observed. The ethyl acetate extract (125 mg/kg ip) showed significant anti-inflammatory activity in carrageenan-induced rat paw oedema model. The exhibited antioxidant activity of ethyl acetate extract of A. candidus broth filtrate was comparable with BHA and ascorbic acid, while anti-inflammatory activity was comparable with standard diclofenac sodium. 相似文献
16.
From among 125 strains of fluorescent and 52 strains of nonfluorescent bacteria initially screened in the laboratory for their
antibiosis towards the bacterial wilt pathogen, Pseudomonas solanacearum, strain Pfcp of Pseudomonas fluorescens and strains B33 and B36 of Bacillus spp., were chosen and evaluated further in greenhouse and field tests. Pfcp treated banana (Musa balbisiana), eggplant and tomato plants were protected from wilt upto 50, 61 and 95% in greenhouse and upto 50, 49 and 36% respectively
in field. Protection afforded by the Bacillus strains was lower. In bacteria-treated plants which were subsequently inoculated
with P. solanacearum plant height and biomass values increased and were close to those of nontreated and noninoculated control plants. 相似文献
17.
Irradiation of the infective larvae of Gaigeria pachyscelis Railliet and Henry, 1910 with gamma rays upto 160 Kr had no significant effect on the in vitro survival of these larvae for a period of 49 days, maintained either at room temperature (32.2--39.4 degrees C) or at 4 degrees C. However, the behaviour of the irradiated larvae in the lamb host was much changed, as shown by a marked reduction in the worm establishment and the development of stunted and sterile worms from these larvae. As the level of irradiation increased, there was a corresponding decrease in the subsequent worm establishment. It was found that male larvae are more sensitive to the effects of irradiation than female ones, particularly at higher levels. 相似文献
18.
19.
This article deals with the design and operation of a filtration probe for estimating cell concentrations in fermentations involving mycelial organisms such as Penicillium chrysogenum. This device, a much improved version of one developed previously, is placed directly into the fermentor and measures the filtration properties of the fermentation broth, which are correlated to the cell concentration. The probe is connected to a computer system which enables on-line estimation of the cell concentration. Fiber-optic light guides are used in combination with a pair of light sources and receivers to detect the relative position of the filter-cake-fermentation-broth interface. The lower sensor in the filtration tube enables reliable estimation of cell mass upto about 20 g/L (dry wt), while the upper sensor yields useful measurement of the filtration properties upto a concentration of about 40 g/L (dry wt) and appears to have the potential to go even higher. Major advantages of this probe system are the ease of measurement and the on-line ability to measure very frequently, due to its nondestructive mode of operation. Thus, the probe obviates the need for manual sampling and reduces the risk of contaminating the culture. But, more significantly, it enables one to implement on-line schemes including feedback control. 相似文献
20.
Summary The effect of three pesticidesviz, simazine, lindane and ceresan upto 100-fold of field application was studied on organic matter mineralization as well as on nitrification rates in Delhi alluvial soil. The normal rate of simazine (2 ppm) did not influence CO2 evolution but higher concentrations suppressed it. Lindane and ceresan inhibited CO2 production from soil by normal concentrations but the same CO2 production was enhanced in berseem roots treated soil upto 10 ppm of ceresan.The nitrification of ammonium sulphate in soil due to these pesticides was impaired significantly for varying periods. The inhibitory effect of higher concentrations was marked only upto 3 weeks, subsequently the rate of nitrification was restored. Among the nitrifiers, Nitrobacter were more susceptible as compared to Nitrosomonas. 相似文献