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1.
《Molecular & cellular proteomics : MCP》2019,18(5):854-864
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- •Zero-length chemical cross-linking of APOA1 peptides in HDL.
- •Cross-links match antiparallel isomers of APOA dimers in molecular modeling.
- •Identical MS/MS spectra of native and synthetic cross-linked peptides.
- •First biochemical evidence of LL5/5 and LL5/4 isomers in human HDL.
2.
《Molecular & cellular proteomics : MCP》2018,17(12):2347-2357
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- •Reported proteasomal spliced HLA peptides do not fit the consensus binding motifs.
- •Their MS/MS spectrum matches suggest that many of them are ambiguous.
- •Our workflow is based on de novo sequencing, alignment, and multiple search tools.
- •The upper bound proportion of cis-spliced peptides is 2–6% and likely much smaller.
3.
《Molecular & cellular proteomics : MCP》2019,18(4):686-703
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- •nLC-MS/MS method to analyze immunoglobulin (Ig) N-glycopeptides from human serum.
- •Multi-isotype, site-specific characterization of immunoglobulin N-glycosylation.
- •IgA2 sequence and glycosylation-site variant analyses.
- •Platform to define disease-specific N-glycan signatures for different Ig isotypes.
4.
《Molecular & cellular proteomics : MCP》2018,17(12):2534-2545
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- •Online PASEF achieves greater than 100 MS/MS per second at full sensitivity.
- •Accurate label-free quantification of over 6000 proteins in 2 h.
- •High throughput demonstrated on 50 ng digests measured in 5 min.
- •High-precision determination of 100,000 peptide collisional cross sections.
5.
《Molecular & cellular proteomics : MCP》2019,18(6):1138-1156
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- •MS-based method to detect native arginine-GlcNAcylation during Salmonella infection.
- •Demonstration that SseK1 targets TRADD, whereas SseK3 targets the death receptors, TNFR1 and TRAILR.
- •Evidence that effector overexpression results in misleading identification of host targets.
- •Crystal structure of SseK3.
6.
《Molecular & cellular proteomics : MCP》2019,18(4):704-714
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- •The proteomes of L. lactis MG1363 and phage p2 at different stages of infection were characterized.
- •16% (226/1412) of the bacterial proteins detected were unique to infected cultures.
- •A targeted approach using synthetic peptides improved the coverage of phage p2 proteome.
- •By means of proteogenomics, we uncovered a conserved phage protein coded by a previously unannotated gene.
- •Deletion of the bacterial gene llmg_0219 (unknown function) impedes phage p2 infection.
7.
《Molecular & cellular proteomics : MCP》2019,18(1):151-161
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- •New MALDI MS imaging sample preparation workflow reveals tissue protease activity.
- •Differential time- and inhibitor concentration-dependence confirm active proteases.
- •Mouse gastric tumor displays high protease activity compared to surrounding tissue.
- •Proteomic data and biochemical protease activity assay support MALDI MSI results.
8.
《Molecular & cellular proteomics : MCP》2019,18(8):1669-1682
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- •Spatiotemporal microproteomics analysis of TBI.
- •Injury site microproteomics reveal distinct phases in 10-day frame post TBI.
- •Uninjured proteomic profile is restored in TBI at 10 days post injury.
- •Substantia nigra protein post 3 days suggest link to Parkinson's disease.
9.
《Molecular & cellular proteomics : MCP》2019,18(1):41-50
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- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
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11.
《Molecular & cellular proteomics : MCP》2018,17(12):2412-2433
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- •Proteogenomics and secretome comparison of human and zoonotic Staphylococcus aureus lineages.
- •869 secreted proteins identified in eight S. aureus isolates of CC8, CC22 and CC398.
- •CC398 lower secretion of surface proteins and higher secretion of hemolysins and exoenzymes.
- •Regulatory differences in the secretomes could be linked to lower SigB activity in CC398.
12.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
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- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
13.
《Molecular & cellular proteomics : MCP》2019,18(5):818-836
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- •Kallikrein-related peptidase 7 is over expressed in ovarian cancer.
- •Quantitative PROTOMAP and TAILS approaches identified putative substrates of KLK7.
- •Pro-MMP10 is activated by KLK7.
- •KLK7 cleaves thrombospondin 1 and IGFBP6 in vitro.
14.
《Molecular & cellular proteomics : MCP》2019,18(11):2207-2224
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- •Quantitative proteomes and epigenetic regulation of T. gondii.
- •Protein crotonylation and 2-hydroxyisobutylation in phenotypically different T. gondii parasites.
- •Regulation of invasion regulation of T. gondii by protein modification.
- •Lysine crotonylation and 2-hydroxyisobutylation regulated in multiple biological processes in phenotypically different T. gondii parasites.
15.
《Molecular & cellular proteomics : MCP》2019,18(9):1782-1795
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- •MEEC are a reliable endocardial in vitro model.
- •Quantitative proteomics to characterize the NOTCH-driven endocardial secretome.
- •NOTCH pathway status underlies different paracrine biological functions.
- •New insights into secreted factors involved in cardiac valve development.
16.
《Molecular & cellular proteomics : MCP》2019,18(6):1036-1053
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- •Establishment of a flow system allowing multi-omics analysis of S. aureus biofilms.
- •Biofilm proteome profiling (intracellular and ECM) plus metabolic footprint analysis.
- •Virulence factors and ribosomal proteins stabilize the ECM as moonlighting proteins.
- •They act as electrostatic bridges between anionic cell surfaces, eDNA and metabolites.
17.
《Molecular & cellular proteomics : MCP》2019,18(4):657-668
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- •Global proteomic remodeling alters antibiotic susceptibility in K. pneumoniae.
- •Drug specific transport, sugar utilization, central metabolism, capsule synthesis.
- •Common pathways of reactive oxygen scavenging, turnover of misfolded proteins.
- •Integrated adjustments and unique drug-specific features for drug combinations.
18.
《Molecular & cellular proteomics : MCP》2019,18(2):200-215
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- •A global lysine succinylome was investigated in A. hydrophila.
- •The lysine succinylation modifications play crucial role on various metabolic pathways.
- •Reversible succinylation on Lys23 and Lys30 regulates the activity of S-ribosylhomocysteine lyase LuxS.
- •Lysine succinylation modifications of LuxS affect quorum sensing and metabolism.
19.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
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- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
20.
《Molecular & cellular proteomics : MCP》2019,18(5):1010-1026
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- •Comparing proteolytic digestions and precursor fragmentation methods for MS of ADPr
- •Identification of 11,265 unique ADPr-modified peptides
- •Mapping of hundreds of peptides co-modified by phosphorylation and ADPr
- •ADPr modification of specific residue types displays spatial preferences