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1.
《Molecular & cellular proteomics : MCP》2018,17(12):2518-2533
Highlights
- •Chromobodies are stabilized by antigen binding in live cells.
- •Monitoring changes of endogenous protein levels in living cells with chromobodies.
- •Broadly applicable system to generate turnover-accelerated chromobodies.
- •Quantification of time- and dose-dependent compound effects.
2.
《Molecular & cellular proteomics : MCP》2019,18(9):1705-1720
Highlights
- •Quantitative changes in global proteome and ubiquitinome in Huntington's disease.
- •Differential ubiquitination of wild-type and mutant Htt in mice brain.
- •Enriched pathways include vesicle transport and mRNA processing.
- •Correlation between protein and diGly site fold changes.
3.
《Molecular & cellular proteomics : MCP》2018,17(11):2242-2255
Highlights
- •BioID and IP-MS were conducted to generate a global ZIKV-host protein interactome
- •Interactome consists of >3000 high confidence ZIKV-host protein interactions
- •Data mining indicates that ZIKV proteins interact with multiple host cell organelles
- •An important role for peroxisomes in ZIKV infection is uncovered
4.
《Molecular & cellular proteomics : MCP》2019,18(11):2335-2347
Highlights
- •Single-pot workflow for manual or automated enrichment of N-terminal peptides.
- •Sensitive enrichment of protein N termini from 10,000 cells or 2 μg crude proteome.
- •Data independent acquisition improves precision of peptide level quantification.
- •First degradomic analyses of sorted immune cells, single seedlings, and mitochondria from patient cells.
5.
《Molecular & cellular proteomics : MCP》2019,18(4):806-817
Highlights
- •Retention time shift can lead to inversion of elution order of peptides.
- •Global alignment methods are suboptimal for alignment of distant runs.
- •DIAlignR employs hybrid (global + local) RT alignment approach.
- •DIAlignR can align swapped peaks accurately across distant runs.
6.
《Molecular & cellular proteomics : MCP》2019,18(11):2285-2297
Highlights
- •BioID with Golgi fractions identified C10orf76 as proximal to GBF1.
- •Tagged C10orf76 overlaps with Golgi markers.
- •C10orf76 binds GBF1 and exchanges rapidly between free and bound forms.
- •C10orf76 is essential for maintenance of the Golgi and for secretion.
7.
《Molecular & cellular proteomics : MCP》2019,18(2):200-215
Highlights
- •A global lysine succinylome was investigated in A. hydrophila.
- •The lysine succinylation modifications play crucial role on various metabolic pathways.
- •Reversible succinylation on Lys23 and Lys30 regulates the activity of S-ribosylhomocysteine lyase LuxS.
- •Lysine succinylation modifications of LuxS affect quorum sensing and metabolism.
8.
《Molecular & cellular proteomics : MCP》2019,18(1):127-150
Highlights
- •Quantitative proteomes of the 2016 WHO Neisseria gonorrhoeae reference strains.
- •Novel gonorrhea vaccine candidates and potential global proteomic AMR markers.
- •First large-scale proteomic profiling of gonorrhea vaccine candidates and AMR.
- •A reference proteomics databank for gonococcal vaccine and AMR research endeavors.
9.
《Molecular & cellular proteomics : MCP》2019,18(12):2516-2523
Highlights
- •Open source software for comprehensive HDX-MS data analysis.
- •Automatic back-exchange correction options.
- •Rigorous statistical analysis of the significance of uptake differences.
- •High quality visualization tools.
10.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
Highlights
- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
11.
《Molecular & cellular proteomics : MCP》2019,18(8):1669-1682
Highlights
- •Spatiotemporal microproteomics analysis of TBI.
- •Injury site microproteomics reveal distinct phases in 10-day frame post TBI.
- •Uninjured proteomic profile is restored in TBI at 10 days post injury.
- •Substantia nigra protein post 3 days suggest link to Parkinson's disease.
12.
《Molecular & cellular proteomics : MCP》2019,18(1):115-126
Highlights
- •Application of Sortase A to label protein N-termini across the whole proteome.
- •Novel gel, proteomic and ELISA-based methods to determine N-myristoylation of proteins in cells, without metabolic labelling.
- •Side by side mass spectrometric quantification of changes in protein N-myristoylation by two complementary methods.
- •Improved Biotin-Neutravidin affinity enrichment protocol.
13.
《Molecular & cellular proteomics : MCP》2019,18(7):1285-1306
Highlights
- •Identification of previously undetected chloroplast envelope proteins.
- •Up to date manual annotation of genuine (or shared) envelope components.
- •New hypotheses for localizations, functions, interactions among cell compartments.
- •A new resource of significant value to the broader plant science community.
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16.
《Molecular & cellular proteomics : MCP》2019,18(5):818-836
Highlights
- •Kallikrein-related peptidase 7 is over expressed in ovarian cancer.
- •Quantitative PROTOMAP and TAILS approaches identified putative substrates of KLK7.
- •Pro-MMP10 is activated by KLK7.
- •KLK7 cleaves thrombospondin 1 and IGFBP6 in vitro.
17.
《Molecular & cellular proteomics : MCP》2018,17(12):2508-2517
Highlights
- •Selective release of IgG Fc glycans in crude serum by endoglycosidase S.
- •CE-LIF-based measurements of IgG undergalactosylation levels (UGS).
- •UGS as a biomarker: Stratification of non-alcoholic steatohepatitis patients and controls.
18.
《Molecular & cellular proteomics : MCP》2019,18(4):669-685
Highlights
- •quantitative phosphoproteome analysis of TDM-activated macrophages.
- •distinct Mincle-dependent and independent phosphorylation and gene regulations.
- •Mincle-dependent activation of PI3K/AKT signaling by TDM.
- •Mincle-independent macrophage response is linked to cell cycle regulation.
19.
《Molecular & cellular proteomics : MCP》2019,18(8):1511-1525
Highlights
- •First global study on laryngeal cells cultured with cigarette smoke enriched medium.
- •Vocal fold fibroblasts increased production of ECM component hyaluronan.
- •Expression of several fibrillar collagens was reduced.
20.
《Molecular & cellular proteomics : MCP》2018,17(12):2496-2507
Highlights
- •Quantitative (phospho)proteome analysis of antibiotic treatment in E. coli.
- •Largest bacterial phosphorylation catalogue.
- •Specific phosphorylation motifs changes during resistance development.
- •Phosphorylation mediated signaling could be a potential target for drug design.