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Seventy-six novel microsatellite markers with various simple sequence repeat (SSR) motifs are reported in this paper. They
were generated on the basis of non-radioactive library screening procedures from flow-sorted rat Chromosome (Chr) 5-specific
DNA, and were mapped in three rat backcross populations. Fifty-four of these markers mapped to Chr 5, while the other 22 mapped
to other chromosomes of the rat genome. The marker D3Uwm8 is a new microsatellite marker for the rat syndecan 4 (ryudocan) gene. A genotyping protocol based on agarose gel electrophoresis
is also provided in this paper.
Received: 17 December 1998 / Accepted: 17 February 1999 相似文献
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