Effect of spatial and temporal patterns of stress and disturbance intensities in a sub-Mediterranean grassland

Abstract

The present study examined a sub-Mediterranean pastoral system in the central Apennines (Italy) with a long history of grazing, where winter cold stress is alternated with summer drought stress. The research goals were to ascertain whether different floristic structures correspond to different stress conditions (xeric and semimesic), and whether peculiar functional plant traits (such as avoidance and tolerance mechanisms) respond to stress/disturbance intensities, and understand how vegetation reacts to changeable livestock pressure (through floristic and plant trait variations). Cluster analysis indicated that separate communities develop under different stress intensities. Other analyses highlighted how avoidance strategies predominate within the pastoral system. Observations of grazed and ungrazed patches conducted in 10-m transects revealed spiny cushion formation in semimesic grassland, where a brief period of overgrazing occurs in late summer, causing variations in plant community structure. All these results confirm the importance of historical grazing and current land use, showing how small disturbances and stress variations cause ecosystem responses. Best practices for management were identified. In xeric conditions, it is advisable that the intensity of disturbance be lessened, while in semimesic grassland overgrazing should be forbidden during the dry period, because it could facilitate the development of spiny patches, and subsequent spread of Brachypodium rupestre.     

Responses of grassland ecosystems to precipitation and land use along the Northeast China Transect

Abstracts. The Northeast China Transect (NECT) has been used to study how water availability influences the composition of plant functional types, soil organic matter, net primary production, trace gas flux, and land‐use patterns. We discuss relations of plant species number, soil C and N and above‐ground biomass with a precipitation gradient and interactions with land‐use practices (grassland fencing, mowing and grazing), on the basis of data from the west part of NECT. The results indicate: 1. The above‐ground biomass of grassland communities has a linear relationship with precipitation under three land‐use practices, while plant species number, soil C, and total soil N have linear relationships with precipitation under fencing and mowing; under grazing the relationships are non‐linear. 2. Plant species number, soil C and total soil N have strong linear relationships with above‐ground biomass under both fencing and mowing, while they seem to have nonlinear relationships under grazing. 3. Land‐use practices along the precipitation gradient result not only in changes in grassland communities but also in qualitative changes of their structure and function. 4. Grasslands are more vulnerable to changes in climate under mowing than under fencing, and are more capable to store C in soil and plants. 5. At a given precipitation level, number of plant species, above‐ground biomass, and soil C are higher under low to medium intensity of human activities (mowing and grazing). A better understanding of how different intensities of human activities will affect the structure and function of grassland will require further research.     

Linking landscape history and dispersal traits in grassland plant communities

Dispersal limitation and long-term persistence are known to delay plant species’ responses to habitat fragmentation, but it is still unclear to what extent landscape history may explain the distribution of dispersal traits in present-day plant communities. We used quantitative data on long-distance seed dispersal potential by wind and grazing cattle (epi- and endozoochory), and on persistence (adult plant longevity and seed bank persistence) to quantify the linkages between dispersal and persistence traits in grassland plant communities and current and past landscape configurations. The long-distance dispersal potential of present-day communities was positively associated with the amounts of grassland in the historical (1835, 1938) landscape, and with a long continuity of grazing management—but was not associated with the properties of the current landscape. The study emphasises the role of history as a determinant of the dispersal potential of present-day grassland plant communities. The importance of long-distance dispersal processes has declined in the increasingly fragmented modern landscape, and long-term persistent species are expected to play a more dominant role in grassland communities in the future. However, even within highly fragmented landscapes, long-distance dispersed species may persist locally—delaying the repayment of the extinction debt.     

Can edaphic factors demonstrate landscape-scale differences in vegetation responses to grazing?

We focused on land units as landscape characteristics and selected seven typical land units on a land catena comprising two areas of southern Mongolia. Hierarchical analysis was used to test the hypothesis that a land unit’s edaphic factors could explain the differences in vegetation responses to grazing. We established the survey sites at increasing distances from a livestock camp or water point within each land unit, then analysed patterns of change in floristic and functional compositions, vegetation volume and soil properties within each land unit to reveal differences in vegetation responses to grazing. We also examined the variations in floristic and functional compositions across land units to identify the edaphic factors that may underlie these differences. Changes in vegetation and soil properties at increasing distances from a camp or water point within each land unit were into three different patterns. Ordination techniques consistently indicated that land unit groups categorised using edaphic factors corresponded to those categorised using response patterns. Our study revealed that edaphic factors were responsible for the observed landscape-scale differences in vegetation responses to grazing in the study areas. In addition, the mechanisms underlying vegetation responses to grazing may have been primarily determined by edaphic factors.     

Grazing‐induced changes in plant–soil feedback alter plant biomass allocation

Large vertebrate herbivores, as well as plant–soil feedback interactions are important drivers of plant performance, plant community composition and vegetation dynamics in terrestrial ecosystems. However, it is poorly understood whether and how large vertebrate herbivores and plant–soil feedback effects interact. Here, we study the response of grassland plant species to grazing‐induced legacy effects in the soil and we explore whether these plant responses can help us to understand long‐term vegetation dynamics in the field. In a greenhouse experiment we tested the response of four grassland plant species, Agrostis capillaris, Festuca rubra, Holcus lanatus and Rumex acetosa, to field‐conditioned soils from grazed and ungrazed grassland. We relate these responses to long‐term vegetation data from a grassland exclosure experiment in the field. In the greenhouse experiment, we found that total biomass production and biomass allocation to roots was higher in soils from grazed than from ungrazed plots. There were only few relationships between plant production in the greenhouse and the abundance of conspecifics in the field. Spatiotemporal patterns in plant community composition were more stable in grazed than ungrazed grassland plots, but were not related to plant–soil feedbacks effects and biomass allocation patterns. We conclude that grazing‐induced soil legacy effects mainly influenced plant biomass allocation patterns, but could not explain altered vegetation dynamics in grazed grasslands. Consequently, the direct effects of grazing on plant community composition (e.g. through modifying light competition or differences in grazing tolerance) appear to overrule indirect effects through changes in plant–soil feedback.     

Landscape Patterns of Tropical Forest Recovery in the Republic of Palau1

A GIS (geographic information systems) database was constructed from aerial photographs, a vegetation map, and topographic map data of the Ngeremeduu Bay Drainage Area (NBDA), Palau, to examine relationships between upland land cover dynamics, environmental variables, and past land use. In 1992, 82.9 percent of the NBDA was forest, 16.6 percent was grassland, and 0.5 percent consisted of village areas. Between 1947 and 1992, there was a 11.2 percent reduction of grassland area primarily due to a 10.9 percent increase in forest cover. These land cover changes led to larger, more continuous stretches of forest and numerous, highly fragmented grassland patches. Significant relationships (P 0.001) were found between the spatial distribution of forest and grassland cover and slope, elevation, soil pH, and percent soil organic matter. These patterns, however, may have resulted from past farm site selection rather than from ecological relationships. Our results indicate that areas of forest expansion were significantly (P 0.001) associated with the location of abandoned agricultural communities. In addition, over 92 percent of areas of forest expansion occurred within 100 m of established forest. These results suggest that the proximity of established forest facilitate forest recovery following human disturbance.     

Plant composition and diversity in a semi-natural Mediterranean island landscape: the importance of environmental factors

Abstract

Islands crucially contribute to the Mediterranean Basin’s high floristic diversity, which, however, is at risk facing climate and land-use changes. Besides the identification of highly diverse areas, the knowledge about factors favouring diversity is of great importance. We analysed plant species diversity and composition related to environmental factors over varied vegetation units on a former Italian prison island in the northwest of Sardinia. Due to a long history of land use with grazing and later abandonment the nowadays protected island features a semi-natural landscape and can serve as an example for strongly anthropogenic altered insular ecosystems. Floristic composition, soil properties, microclimate and ungulate abundance were assessed. Relationships of vegetation composition and diversity with abiotic variables were examined by Canonical Correspondence Analysis, which indicated the importance of air temperature, soil moisture, slope gradient and C/N ratio for floristic differentiation. Most important abiotic factors for plant species richness were relative air humidity and soil moisture, while floristic diversity was mainly determined by air temperature and pH. Furthermore, observation data pointed to an adverse influence of ungulate abundance for plant species diversity. Regarding nature conservation, grazing intensity thus must be critically taken into account, especially for sensitive vegetation units like the coastal garrigue.     

Environmental causes and consequences of forest clearance and agricultural abandonment in central New York, USA

Aim Climate, topography and soils drive many patterns of plant distribution and abundance across landscapes, but current plant communities may also reflect a legacy of past disturbance such as agricultural land use. To assess the relative influences of environmental conditions and disturbance history on vegetation, it is important to understand how these forces interact. This study relates the geographical distribution of land uses to variation in topography and soils; evaluates the consequences of land‐use decisions for current forests; and examines the effects of agricultural land use on the chemical properties of forest soils. Location Tompkins County occupies 1250 km² in central New York's Finger Lakes region. Like much of eastern North America, this area underwent forest clearance for agriculture during the 1800s and widespread field abandonment and forest recovery during the 1900s. The current landscape consists of a patchwork of forests that were never cleared, forests that developed on old fields and active agricultural lands. Methods We investigated relationships among topography, soils and land‐use decisions by gathering information about land‐use history, slope, aspect, elevation, soil lime content, soil drainage and accessibility in a geographic information system (GIS). To assess the effects of agriculture on forest soil chemistry, we measured pH, organic matter content and extractable nutrient concentrations in field‐collected soil samples from 47 post‐agricultural and uncleared forests. Results Steeper slopes, less accessible lands and lower‐lime soils tended to remain forested, and farmers were more likely to abandon fields that were steeper, farther from roads, lower in lime and more poorly drained. Slope had by far the greatest impact on patterns of clearance and abandonment, and accessibility had a surprisingly strong influence on the distribution of land uses. The effects of other factors varied more, depending for example on location within the county. Current forest types differed accordingly in topography and soil attributes, particularly slope, but they also showed much overlap. Post‐agricultural and uncleared forest soils had similar chemical properties. Forests on lands abandoned from agriculture 80–100 years before had slightly higher pH and nutrient concentrations than adjacent, uncleared forests, but these changes were small compared to environmental variation across the county. Main conclusions Despite differential use of lands according to their topography and soils, the substantial influence of accessibility and the relatively small scale of land‐use decisions allowed for broad similarity among forest types. Thus, the topography and soil differences created by land‐use decisions probably contribute little to landscape‐level patterns of diversity. Subtle changes in forest soil chemistry left from past agriculture may nevertheless affect plant distribution and abundance at finer scales.     

Landscape structure and land use history influence changes in island plant composition after 100 years

Aim We investigated how current and historical land use and landscape structure affect species richness and the processes of extinction, immigration and species turnover. Location The northern part of the Stockholm archipelago, Baltic Sea, Sweden. We resurveyed 27 islands ranging from 0.3 to 33 ha in area. Methods We compared current plant survey data, cadastral maps and aerial photographs with records obtained from a survey in 1908, using databases and a digital elevation model to examine changes in plant community dynamics in space and time. We examined the effects of local and landscape structure and land use changes on plant species dynamics by using stepwise regression in relation to eight local and three landscape variables. The eight local variables were area, relative age, shape, soil heterogeneity, bedrock ratio, number of houses, forest cover change, and grazing 100 years ago. The three landscape variables were distance to mainland, distance to closest island with a farm 100 years ago, and structural connectivity. Hanski’s connectivity measure was modified to incorporate both connectivity and fragmentation. Results The investigated islands have undergone drastic changes, with increasing forest cover, habitation, and abandonment of grassland management. Although the total species richness increased by 31% and mean island area by 23%, we found no significant increase in species richness per unit area. Local variables explain past species richness (100 years ago), whereas both local and landscape variables explain current species richness, extinctions, immigrations and species turnover. Grazing that occurred 100 years ago still influences species richness, even though grazing management was abandoned several decades ago. The evidence clearly shows an increase in nitrophilous plant species, particularly among immigrant species. Main conclusions This study highlights the importance of including land use history when interpreting current patterns of species richness. Furthermore, local environment and landscape patterns affect important ecological processes such as immigration, extinction and species turnover, and hence should be included when assessing the impact of habitat fragmentation and land use change. We suggest that our modified structural connectivity measure can be applied to other types of landscapes to investigate the effects of fragmentation and habitat loss.     

Restoring biopedturbation in grasslands with anthropogenic focal disturbance

Grassland ecosystems evolved with natural disturbance events on multiple spatial scales in which focal, fine-scale soil disturbance by animals often was imbedded within large-scale grazing disturbance. The resulting plant communities adapted to both broad-scale and fine-scale disturbance that resulted in species-rich plant communities. These natural disturbance regimes have been largely replaced by anthropogenic disturbance. While we generally understand grassland response to modern grazing practices, we know much less about plant community response to soil disturbance imbedded within non-focal grazing. Therefore, we used a tracked vehicle to focally disturb soil in a North American mesic mixed prairie that was either undisturbed prairie or prairie with a recent history of disturbance from either grazing or haying. Successional trajectory and recovery time following focal soil disturbance was similar between grazed and hayed plant communities. Species composition did not differ (P < 0.05) between grazed or hayed prairie and the respective undisturbed prairie. Plant species richness and bare ground increased (P < 0.05) following focal soil disturbance in both grazed and hayed communities, but focal soil disturbance combined with either grazing or haying did not change either plant species richness or bare ground more than (P > 0.05) focal soil disturbance alone. Also, the effect of focal soil disturbance was shortlived with recovery in two growing seasons. Our results suggest that anthropogenic focal soil disturbance is a reasonable mechanism to restore soil disturbance to the grassland ecosystem.     

Fractal analysis of plant spatial patterns: a monitoring tool for vegetation transition shifts

Spatial heterogeneity, like species diversity, is an important ecosystem property. We examine the effects of land use on the diversity and spatial distribution of plants in five semi-arid communities of eastern Spain using non-linear methods to assess the spatialtemporal dynamics of plant populations. Specifically, we are interested in detecting long-term structural changes or drift in an ecosystem before it is too late to prevent irreversible degradation. Fractal analysis is used to characterize the complexity of plant spatial patterns and Information Theory indices are used to measure change in information flow with land use changes and soil substrate. We found that grazing favored diversity and heterogeneity of species distribution on the impoverished gypsum and saline substrate community, as opposed to the detrimental effect of grazing in the Alpha steppe community. Indeed, old-field succession after 30 years of abandonment showed a recovery of species diversity but not the spatial structure of the vegetation. Further, Information Fractal Dimension, representing the unpredictability of plant spatial patterns in the landscape, increased as we moved from a highly diverse to a less diverse community, revealing the change to a more scattered and homogeneous spatial plant distribution. The Information Fractal Dimension is a good estimator of ecosystem disturbance, independent of scale, and thus can be used to monitor ecosystem dynamics.     

Cattle foraging habits shape vegetation patterns of alluvial year-round grazing systems

Year-round grazing with robust cattle is increasingly used as a near-natural tool for the restoration of structurally diverse grassland ecosystems in Western and Central Europe. The aim of this study was to evaluate the general success of year-round grazing and to analyze the interplay between emerging vegetation structures, grazing patterns and abiotic environmental conditions. In summer 2010 vegetation composition, aboveground biomass and soil properties were sampled at 44 quadrats of 4 × 4 m² within two year-round grazed floodplain sites in Northwestern Germany. For plot selection, we predefined structural vegetation types and later statistically determined indicative plant species for each structural type. Our results showed that year-round grazing resulted in the successful creation of eutrophic grassland communities on former agricultural land after 15 years. Soil parameters like phosphorous and potassium concentration and the flooding duration did not or only slightly differ between different structure types. In summer, cattle preferably fed at short-growing patches which were of better digestible biomass than taller patches. Hence, our data clearly demonstrate a positive feedback between grazing intensity and fodder quality leading to a patchy vegetation structure of intensively grazed swards and less frequented areas dominated by high-growing grasses and tall forbs, almost independently from primary differences in soil parameters and other site factors such as flooding duration. The remarkable structural and floristic diversity of year-round grazing systems clearly is a result of these spatially contrasting feeding patterns.     

Using past and present habitat to predict the current distribution and abundance of a rare cryptic lizard,Delma impar (Pygopodidae)

We present logistic regression models predicting the distribution and abundance of a threatened cryptic lizard, Delma impar (Pygopodidae), in the Australian Capital Territory (ACT). The models incorporate current habitat and historical land use and habitat change (woodland clearance, ploughing, grazing, fertilizer application). Information on historical land use was acquired from land survey maps, aerial photographs and from floristic indices of land management. Floristic indices were developed from a survey of local agronomists who scored individual plant species, responses to grazing, ploughing and fertilizer application. Floristic indicies proved to be more informative than floristic ordination analyses. It emerged that historical factors were of key importance for predicting the distribution and abundance of D. impar. Since European settlement, D. impar has apparently spread from primary (naturally treeless grasslands) into secondary grasslands (grassland formerly with an overstorey of trees) and has been locally excluded by some farming activities such as ploughing. We conclude that a combination of current habitat and past changes in habitat may be necessary to understand the current distributions of plant and animal species that have limited dispersal ability and that are susceptible to local temporary habitat destruction. Active conservation strategies involving, for example, assisted dispersal, may be important for these species.     

Environmental factors and community dynamics at the southernmost part of the North American Graminetum

We have delineated the spatio-temporal plant assemblages prevailing under contrasting environmental conditions at the southernmost part of the North American Graminetum in central México and discuss the relative importance of these factors in determining plant community structure and composition. 353 line-transect samples were collected during 11 years from exclosures and adjacent grazed areas within three Bouteloua-dominated grasslands and one Acacia schaffneri shrubland and analyzed using TWINSPAN. In addition, eight edaphic variables were utilized to evaluate similarities in soil properties among sites, using unweighted-pair groups method. Results from TWINSPAN were translated into mosaic patterns to show the distribution of communities as a function of environmental factors over time. Under no or moderate grazing, summer precipitation promotes an initial differentiation of vegetation into high, low or average rainfall communities. This situation is altered in grasslands degraded by intensive grazing pressure, where rainfall patterns play a subordinate role to that of livestock herbivory. Soil influences are secondary to those of precipitation and grazing in affecting grassland structure and composition. In shrubland, community stability is related to high floristic and edaphic spatial heterogeneity in the face of climatic variability.     

Management type affects composition and facilitative processes in altoandine dry grassland

We performed our study in the Dry Puna of the southern Peruvian Andes. Through a comparative approach we aimed to assess the effects of the two management systems, low grazing pressure by wild camelids vs. high grazing pressure by domestic livestock and periodic burning. Our general hypothesis was that the traditional high disturbance regime affects the dry Puna species diversity and composition through modifications of the magnitude of plant–plant-interactions and changes of the community structure due to shifts in species dominance. In 40 plots of 10 × 10 m, the cover value of each species was recorded and the species richness, floristic diversity, and community similarity of each treatment were compared. For each disturbance regime, differences of soil features (organic matter, carbon/nitrogen ratio, and potassium content) were tested. To evaluate plant–plant interactions, 4 linear transect divided into 500 plots of 10 × 10 cm were laid out and co-occurrence analysis was performed. We found that different disturbance regimes were associated with differences in the floristic composition, and that the high disturbance condition had lower species diversity and evenness. A decrease of tall species such as Festuca orthophylla and increase of dwarf and spiny Tetraglochin cristatum shrubs was observed as well. In addition, different disturbance intensities caused differences in the functional composition of the plant communities, since species with avoidance strategies are selected by high grazing pressure. High disturbance intensity was also associated to differences of soil features and to different clumped spatial structure of the dry Puna. Our results indicate also that: positive interactions are often species-specific mainly depending on the features of nurse and beneficiary species; the importance of positive interaction is higher at low grazing pressure than at high disturbance intensity; the magnitude and direction of the herbivory-mediated facilitation processes may be traced back to the grazing pressure of wild camelids.     

放牧扰动下高寒草甸植物多样性、生产力对土壤养分条件变化的响应

为了揭示高寒小嵩草草甸群落在放牧扰动下,探讨土壤养分供给水平的变化对生态系统初级生产力和多样性影响,为高寒草地的退化演替机理研究提供依据,以野外样地调查和室内分析法研究了放牧扰动下高寒草甸植物多样性、生产力对土壤养分条件变化的响应.结果表明,放牧干扰不仅改变了高寒小蒿草草甸群落土壤根系和蕴育土壤根系的"载体"量及根土比例,改变了植物群落的结构和功能,而且使土壤的物理和化学特性发生了明显的改变.随着放牧强度的增加,蕴育土壤根系的基质量逐渐减少,根土比特别是0～10 cm土层的根土比例增加;"载体"量减少导致大部分地下根系由于营养供给水平的降低而死亡,归还土壤中有机质的数量逐渐减少,加之地上部分持续利用,土壤养分也在不断消耗,土壤基质量的减少和土壤资源持续供给能力的下降,草地发生逆向演替(退化),表现在:物种数减少、多样性下降、能量的分配转向地下等;土壤性状上的某些改变(土壤容重、土壤湿度等),也会引起植被组成、物种多样性变化;放牧主要通过影响土壤环境及其养分含量来改变草地群落生物量(地上、地下);土壤表面的适度干扰和原有植物的适度破坏为新成员提供了小生境,从而允许新的植物侵入群落,并提高了植物的丰富度.但是,在受到强度干扰时,草地植物群落的主要物种的优势地位发生明显的替代变化.     

内蒙古典型草原物种组成和群落结构对长期放牧的响应

该文比较研究了内蒙古羊草草原和大针茅草原放牧演替系列经过20a (1985~2005年) 放牧, 利用群落组成与结构的变化分析了这两个放牧演替系列上15个植物群落的变化趋势, 并依此探讨长期放牧对草原生态系统结构和功能的影响。结果表明:在大针茅 (Stipa grandis) 牧压梯度系列上, 群落仍以大针茅群系为主, 冷蒿 (Artemisia frigida) 为建群种的群落消失;在羊草 (Leymus chinensis) 牧压梯度系列上, 羊草和冷蒿不再是建群种, 被西伯利亚羽茅 (Achnatherum sibiricum) 、大针茅、冰草 (Agropyron cristatum) 和糙隐子草 (Cleistogenes squarrosa) 所替代。综合两个牧压梯度系列的研究结果得出以下基本结论:长期过度放牧进一步加速了草原群落的退化进程, 但退化草原具有较高的恢复弹性, 控制放牧使部分群落得到一定程度的恢复。同一植物群落时间变化, 或不同演替阶段的植物群落在空间序列上的位移程度主要取决于放牧史和利用强度。过去20a中, 特别是2003年以来草原管理政策的改变并没有导致该区域草地的整体退化, 而是在一定程度上有利于草地的恢复。     

Impact of land use on plant biodiversity and measures for biodiversity conservation in the Loess Plateau in China – a case study in a hilly-gully region of the Northern Loess Plateau

The Loess Plateau is a special natural–cultural unit in northern China. Intensive land use in the past has had, and forestation and grass planting at present will have inevitable impacts on plant biodiversity in the Loess Plateau. Based on the analysis of floristic features within three sampling sites with different land use practices and analysis of species richness among different land use types, we discuss impacts of land use on species richness and floristic features in the Northern Loess Plateau. The results drawn from this case study are as follows: (1) It appears that forestation and grass planting have had a positive influence on the local species diversity, but they have contributed little to the native vegetation in terms of conserving its floristic features. (2) Caragana intermedia shrubland, Pinus tabulaeformis forestland, and natural grassland have made important contributions to supporting indigenous species and maintaining local plant biodiversity. (3) There is a significant positive correlation between land use diversity and species richness. These results imply that practicing biodiversity conservation in situ is feasible and the suitable choice for the Loess Plateau. Concrete measures for biodiversity conservation in the area can include setting up small nature reserves and diversifying land use patterns to maintain as much habitat as possible for native vegetation. The artificial Hippophae rhamnoides shrubland should not be further promoted, considering its negative influence on biodiversity conservation.     

Grazing and the vanishing complexity of plant association networks in grasslands

Interactions are key drivers of the functioning and fate of plant communities. A traditional way to measure them is to use pairwise experiments, but such experiments do not scale up to species-rich communities. For those, using association networks based on spatial patterns may provide a more realistic approach. While this method has been successful in abiotically-stressed environments (alpine and arid ecosystems), it is unclear how well it generalizes to other types of environments. We help fill this knowledge gap by documenting how the structure of plant communities changes in a Mediterranean dry grassland grazed by sheep using plant spatial association networks. We investigated how the structure of these networks changed with grazing intensity to show the effect of biotic disturbance on community structure. We found that these grazed grassland communities were mostly dominated by negative associations, suggesting a dominance of interference over facilitation regardless of the disturbance level. The topology of the networks revealed that the number of associations were not evenly-distributed across species, but rather that a small subset of species established most negative associations under low grazing conditions. All these aspects of spatial organization vanished under high level of grazing as association networks became more similar to null expectations. Our study shows that grazed herbaceous plant communities display a highly non-random organization that responds strongly to disturbance and can be measured through association networks. This approach thus appears insightful to test general hypotheses about plant communities, and in particular understand how anthropogenic perturbations affect the organization of ecological communities.