Appunti Sulla Flora Stradale E Ruderale Dei Dintor Ni Di Molfetta (Bari)

Summary

The author has studied the flora of a few country lanes in the surroundings of Molfetta (Apulia). The various stations are described and the plants collected are listed.     

Influenza Dei Tegumenti Dell'Embrione Sull'Azione Dei Raggi X In Semi Di Triticum Durum Desf

Abstract

Influence of seed coats on the effect of X-rays on the embryo of «Triticum durum» Desf. – Seeds of «durum» wheat «Cappelli», presoaked in distilled water at 20° C. for 24 h in the dark, were treated with X-rays 0.5, 1, 2, 4 and 6 KR. Before irradiation, seeds were distributed among the following three groups: 1) normal seeds with covered embryos; 2) seeds to irradiate and afterwards to submit to the removal of seed coats (pericarp and spermoderm) on the embryo; 3) seeds to irradiate after having removed seed coats on the embryos. Of course, for every group, the respective unirradiated controls were prepared. The material was sown in sterilized sound and kept in termostatic chamber at 20°C in the dark, for 7 days. After 4 and 6 days the seedling emergence was registered, after 7 days seedling were measured. Of particular interest is the remarkable depressing effect on the growth of seedlings coming from bared and irradiated embryos: this effect is induced – for significant differences, as compared with the behaviour of materials of groups 1 and 2 – especially on the root, by X-rays 2, 4 and 6 KR. No difference there is, on the contrary, among the seedlings coming from irradiated and bared embryos and those coming from irradiated controls.     

L'Evoluzione Dei Cloroplasti Durante L'Inverdimento Sperimentale Di Frammenti di Tuberi di Topinambour (Helianthus Tuberosus)

Abstract

Lembotropis emeriflorus (Rchb.) Skali?ka in the Carnian Alps too.—Lembotropis (=Cytisus) emeriflorus, endemic species between the lakes of Lugano and Como, occurs also in the Southern Carnian Alps, where it grows either on gravelly habitats or on rocky slopes or under Austrian pine woods of the mountain zone. After this discovery its distributional pattern is now a typical bicentric one.     

Pugillo Di Licheni Dell'Abissinia E Dell'Eritrea

Abstract

The long pollen sequences from four volcanic lakes of the Lazio région are presented in order to outline the history of flora and vegetation on the Tyrrhenian side of Central Italy during the last hundred thousand years. The four long pollen records correlate satisfactorily with each other and reflect climatic changes at least at regional scale. Particular attention is paid to the development of arboreal vegetation during the last glacial. The local name “Lazio Complex” is given to the seven main fluctuations of trees of the Pleniglacial (from about 60,000 to about 30,000 years B.P.). The history of Zelkova, Abies, Picea, Fagus, Corylus, Ulmus, Carpinus betulus, Tilia in central Italy is outlined.     

Osservazioni Sul Meccanismo Di Conversione Della Energia Luminosa In Energia Chimica Nel Processo Fotosintetico

Abstract

Contribution to the knowledge of the mechanism of light energy conversion into chemical energy during the photosinthetic process. — A general scheme for the pathways of electron transport in chloroplasts is discussed, in the light of the recent evidence for the existence of cyclic photophosphorylation in vivo. The possible function of cytochrome f is discussed.     

Ciclo Di Accrescimento E Differenzazione Delle Gemme in Piante Perenni Nel Territorio Di Bari

Summary

The evolution of cambial activity during one year in Viburnum Tinus L. in Bari has been studied. The research seems to be particularly difficult in this evergreen shrub. The wood is of the porous diffused type with scarse evidence of wood rings. The vessel diameter varies rather irregularly in the wood ring; on the other side the fibers show wide variations and may be assumed as a good index of the wood ring evolution. Both in the branch and in the stem only one wood ring each year is formed.

Cambial activity prosecutes during the whole year, with an irregular step. During the period July-beginning of September the cambium devides very slowly, or possibly stops deviding.

The early wood is produced earlier in the branch than in the stem; namely in February-end of May in the branch and in March-beginning of June in the stem. The stimulating growth stuffs evidently proceeds downwards from the top to the base of the plant. The relations between ring evolution and climatic factors are discussed. The peculiar cambial poussée during the month of June seems to be correlated with the exceptionally aboundant rainfall of May in Puglia in 1947.

The late wood is formed during the other months discontinuosly. The alternation between the two phases of cambium division and wood lignification has been focussed. The wood ring in Viburnym Tinus is annual and the early wood differentiates in spring.     

Ciclo Di Accrescimento E Differenziazione Delle Gemme in Piante Perenni Nel Territorio Di Bari

Abstract

The secondary wood of the fig tree is characterized by a remarcable degree of dimorphism and the concentric rings d o not correspond to yearly recurring growth periods. These false rings are formed by alternating tangential strips of fibers and wood parenchima. During each vegetative season three or four of these strips arc laid down by the cambium.

During the year the cambium undergoes a period of activity and a period of rest, but there is no histological difference between the early wood and the late one, differentiated just before the restring period. In consequence the so-called annual wood rings do not exist in the fig tree stem.     

L'amido E L'endosperma Delle Cariossidi Dure E Tenerizzate Di Triticum Durum Desf

Abstract

Starch and endosperm of vitreous and starchy (yellow-berries) kernels of Triticum durum Desf. — Starch and endosperm in kernels of T. durum Desf. have been examined in relation with the problem of the origin and nature of the vitreous or starchy texture which this tissue shows in the ripe fruits. This research has demonstrated that the starch of the vitreous differs from that of the starchy kernels in the shape, respectively irregular and regular; in the distribution of the frequencies y versus the length of the diameter x which is exponential and of the type log y = log a — x log b, in the vitreous kernels the value of log b is generally lower than in the starchy kernels; in the submicroscopical texture, because the irregular grains of the vitreous kernels do not show either birefringence or polarisation cross and are coloured with Congo red: starchy kernels behave quite differently. The starchy kernels are also more swollen and regular, their apparent specific weight and their nitrogen content being less than in the vitreous; the process of starchiness is always in close contact with fibro-vascular bundle of the row; in the starchy cells the cytoplasm shows some interruptions of continuity which leave partly uncovered the starch grains; the endosperm and the starch of the hard kernels wetted with water or placed in moist atmosphere become micro- and macroscopically similar to the starchy ones; in the starch the process of trasformation of the orderly into the amorphous texture is reversible, but for this to happen the only contact with water is insufficient; according to the treatments the vitreous kernels might show a decrease in their nitrogen content. Both starch types are connected with the presence orabsence of slightly bound water; the hypothesis which considers the nitrogen supply of the fruit as immediate cause of the endosperm texture is criticised and it is considered more plausible that such cause might be the different structure and texture of the cytoplasmatic gels during, in function of temperature and moisture.     

Effetto Del Dinitrofenolo Sulla Conversione Del Glicerolo A Glucidi in Fettine Di Carote E in Internodi Di Pisello

Abstract

On the effect of dinitrophenol on carbohydrate activation in higher plant tissues. — Previous investigations on the effects of 2,4 dinitrophenol (DNP) on carbohydrate metabolism in isolated pea internodes and in yeast showed that the increased rate of glycolysis induced by the uncoupler corresponds to an increased rate of the conversion of free hexoses and polysaccarides to hexose phosphates. In yeast about 30% of the radioactivity supplied and taken up as ¹⁴C labelled glucose, and 20% of that supplied and taken up as glycerol is recovered as soluble sugar and glycogen; this phenomenon is almost completely suppressed by 10^-4M DNP.

This suggested that a mechanism involving kinase enzymes, on one hand, and phosphatases, on the other, is mediating the interconversion of phosphorylathed and free sugars, and that the apparent increase of hexose phosphorylation observed in the presence of DNP might depend on a decreased rate of phosphatase mediate reactions, consequent to the decrease of phosphorylated sugars level in the cell.

The experiments here reported were planned to test the validity of this hypothesis in the case of higher plant tissues.

Material used in these experiments were segments from the growing part of the third internode isolated from 7 day old, etiolated pea seedlings, and carrot root diks (0,7 mm thick, 7 mm diameter) preincubated for 24 hours in aerated distilled water. Both of these materials show an active, steady respiration and some growth activity, so that they may be taken as representing a condition close enough to that of the generally physiologically active higher plant tissues.

The reversibility of the hexose phosphate-free sugar interconversion process was tested by feeding 10^-3M 1-C¹⁴ labeled glycerol, and measuring after 150 minutes the amount of radioactivity incorporated into CO₂, soluble sugars, organic acids and proteins. The results of these experiments are summarized in table I and II.

Glycerol metabolism as well as its response to DNP appears very similar in the two material used. In both cases, glycerol uptake and incorporation into organic acids and amino acids is almost insensitive to DNP. In contrast large differences are observed for the free sugar fraction. In the absence of the uncoupler, a consistent amount of the radioactivity fed as glycerol is found in this fraction. It appears reasonable to assume that the glycerol-sugar interconversion comprehends, as intermediate steps, glycerol-P, fructose di-P (or sedoeptulose di-P) and hexose-6-P. If this is true, the observed data implicate that a continuous interconversion occurs, in the cell, between sugar phosphates and free sugars and vice-versa, one reaction direction involving the activity of phosphatases, and the other one that of kinases. The true rate of this interconversion process is probably much larger than indicated by the radioactivity found in free sugars: as a considerable part of the triose-P transormed into sugars must immediately re-enter the descending flux of glycolysis.

This view finds some support in the fact that DNP almost completely inhibits the incorporation of radioactivity in the free sugar fraction. It has been previously observed that DNP very markedly decreases the level of hexose mono- and di-phosphates and of triose-phosphates in the pea stem tissues. If phosphatases acting on fructose di-phosphate and on hexose-6-P are not saturated by their substrates, a decrease of the rate of free hexose synthesis from sugar phosphates should be expected.

The present results are thus consistent with the hypothesis that hexose phosphates and free sugars in the cell are continuously interconverted by the simultaneous action of phosphatases and kinases; and that the effect of DNP, and thus of any physiological conditions decreasing the ATP/ADP ratio in accelerating free hexose utilizations is at least in part due to a decreased rate of the reactions catalized by fructose diphosphate and hexose-6-P phosphatases. The reversibility of the kinase-phosphatase system would thus represent a crucial link in the mechanism by which the rate of carbohydrate activation and breackdown is controlled by the rate of utilization of high-energy phosphate bonds.     

Sviluppo Vegetativo E Riproduttivo Di Alcune Longidiurne in Rapporto All'Azione Dell'Acido Gibberellico

Summary

Continuing the experiments on the action of Gibberellic acid on growth and development of herbaceous plants, the Authors have examined once more several species which require “long day” for espletion of some phases of their reproductive development.

The principal aim was to bring into evidence whether Gibberellic acid can really substitute for some manifestations of the reproductive development itself, or whether some apparent manifestations of reproductive development provoked by this substance are due primarily to modifications of vegetative processes determinated by it.

The experiments were carried out on Papaver somniferum L., Centaurea calcitrapa L., Oenothera acaulis L., Aethusa Cynapium L. and Myosurus minimus L.

Results obtained on Papaver somniferum demonstrate that Gibberellic acid accelerates the macroscopic flowering manifestations under short day conditions of plants already induced to flowering, substituting in this for the effect of “long day” factor in so far as concerns the lengthening of the floral axis. No experiments which can establish whether Gibberellic acid may have an action on specific processes involved in the transition to the reproductive stage, have so far been conducted on Papaver.

Experiments on Centaurea calcitrapa have revealed that Gibberellic acid, treatment though promoting in plants manteined in short-day conditions a “bolting” effect simulating that obtained usually only at long-day, does not succeed in flowering this is true in our experimental conditions (experiments limit: 10 weeks; (photo-phase at light intensity inferior to 2000 lux). Experiments on Centaurea were also supplemented with histological observation of esperimental material.

In the experiments on Oenothera acaulis, a long-day plant only for the macroscopic development phase of floral apparatus, and indeterminate for the formation of floral primordia, has been noted that Gibb. acid can, in the long run, partly substitute for the long-day effect. However, development of floral structures at shortday, is more easily obtained if the plants are supplied, besides with Gibberellic acid, also with other stimulating and trophic substances. This and others observations may indicate that Gibberellic acid represent only one of the substances involved in the metabolism of plants under long-day conditions.

Aethusa Cynapium has furnished results similar to those obtained with Centaurea, even if within very long experimental limits some apparently indirect action of Gibberellic acid towards flowering may occurs.

Preliminary experimental data on logday Myosurus minimus L. show that Gibberellic acid can substitute sooner or later for longday requirements in flowering. In fact, two months old plants flower when Gibberellic acid is supplied under 9 hr. shortday conditions while the controls in shorday remain vegetative. We must note that Myosurus flowers better when light extension is prevalently constituted by far red radiation the effect of which is apparently substituted more easily by Gibberellic acid even if its action appears less afficient and rapid than that of supplementary illumination.     

Ricerche Embriologiche Sul Genere Thalictrum. II. Embriologia e Cariologia Di Thalictrum Lucidum L. e di Thalictrum Minus L. Subsp. Minus

Abstract

Embryological researches in the «Thalictrum» genus. II. Embryology and caryology of «Thalictrum lucidum» L. and «Thalictrum minus» L. ssp. «minus». — In their development the female gametophytes of Thalictrum lucidum L. and Thalictrum minus L. ssp. minus follow the Normal type. In the female gametophytes of these species several types of antipodal cells occurs such as: their considerable enlargement, formation of many antipodals polyploid or polinucleated antipodal cells. In these various types, however, a rapid regression of the antipodals occurs. In T. lucidum some tendency to a disposition of the tetrapolarized type has been verified. The chromosome number has been confirmed to ben 2n=28 in besides, in the pollen development, many cases of regression of the microspores has been verified. The chromosome number has been confirmed to ben 2n=28 in Thalictrum lucidum L., and has been found to ben 2n=14 in Thalictrum minus L. ssp. minus, a new number in this species, which was earlier reported to have 2n=42.     

Studio Comparativo della Struttura e del Ciclo Biologico di Due Diatomee di Acqua Dolce: Navicula Minima Grun. E Navicula Seminulum Grun.: III. Esame Dei Caratteri di Navicula Minima Grun. E di Navicula Seminulum Grun

Abstract

Comparative study of the structure and of the biological cycle of two fresh water diatoms: « Navicula minima » Grun. and « Navicula seminulum » Grun. III. Examination of the characteristics of « N. minima » Grun. and « N. seminulum » Grun. — After studing separately the Navicula minima Grun. and the Navicula seminulum Grun. the various characteristics of the two have been compared and contrasted for purpose of classification. The form and dimentions of the frustules, the density and the structure of the streaks, the structure of the walls, the life circle, aspects of their growths, and some anomalies, most especially, as regards the streaks were examined. It was found the among such numerous characteristics, only one, that is, the presence of the streaks formed by double rows of pores in N. seminulum rather than of a simple file as in N. minima makes one thinks that, in spite of strong similarities existing between these two Naviculae, they are to be regarded as belonging to e different systematic classes. However, their actual positions in taxonomy May-June be defined by further research into the structures and the variabilities of the frustules of other species of Naviculae similar to those examined.