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心脏肾素-血管紧张素系统在游泳引起的生理性心肌肥大中的作用 总被引:2,自引:0,他引:2
本实验采用游泳训练(SW)引起的大鼠心肌肥大模型,通过放射免疫及生化等方法,对生理性心肌肥大时,心脏和循环肾素-血管紧张素系统(RAS)的变化进行了初步研究。观察到游泳五周时,大鼠左、右心室重与体重比值(V/Bwt)显著升高,同时,左、右室心肌血管紧张素Ⅱ(AngⅡ)含量及心肌Ang转换酶(ACE)活性也较对照组明显升高(P<0.05)。心肌AngⅡ与V/Bwt之间存在明显的正相关关系(r=0.7721,P<0.001)。SW组血浆AngⅠ,Ⅱ及肾素活性(RA)与对照组相比无明显差异,其血浆AngⅡ与V/Bwt之间无明显相关性。上述研究提示:心脏RAS在SW引起的生理性心肌肥大中可能起着重要作用,而且这种作用在很大程度上不依赖于循环RAS。 相似文献
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急性压力超负荷后心肌cAMP与肾素血管紧张素系统活化的关系 总被引:1,自引:0,他引:1
为阐明急性压力超负荷后心肌细胞内cAMP浓度升高和心肌肾素血管紧张素系统活化之间是否存在内在因果联系,用腹主动脉缩窄的方法建立急性压力超负荷大鼠模型。发现在术后1h心肌组织中血管紧张素转换酶(ACE)mRNA及蛋白表达均显著增加,ACE活性及血管紧张素Ⅱ(AngⅡ)含量也明显升高,并在高水平。同时,心肌组织cAMP含量于术后0.5h明显增加,术后5d时达峰值,术后30d降至正常。在心肌细胞培养的基 相似文献
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阿尔茨海默病(Alzheimer’s Disease, AD)是一种慢性神经系统退行性疾病,AD的主要病理表现为脑组织中的老年斑和神经纤维缠结,老年斑的主要成分是异常积聚的β-淀粉样蛋白,过度磷酸化的tau蛋白是神经纤维缠结的主要成分。研究发现AD患者脑内微小RNA表达异常,且证据表明微小RNA参与β-淀粉样蛋白过量生成和tau蛋白过度磷酸化等Alzheimer样病理机制,在AD的发病中起着重要作用。本文就微小RNA在tau蛋白过度磷酸化中的作用及机制进行概述。 相似文献
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微小RNA(microRNA,miRNA)是一类长度为22个核苷酸左右的内源性非编码小RNA分子。自1993年最先从秀丽隐杆线虫体内发现miRNA以来,目前为止已有35 000多条miRNA在植物、动物及病毒中被发现。它们作为重要的转录调控因子,参与细胞分化、凋亡、代谢、信号转导、免疫等多种生物学过程。病毒和宿主细胞均可编码miRNA,病毒编码的miRNA可改变宿主内环境,而宿主编码的miRNA则可影响病毒生存。本文就miRNA对病毒与宿主相互作用的调控进行综述。 相似文献
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目的研究5种微小RNA(microRNA)在前列腺癌组织中的表达情况及其临床意义,并探讨它们在前列腺癌诊断中的潜在应用价值。方法应用核酸分子原位杂交(IsH)的方法,结合组织芯片(TMA)技术检测38例良性前列腺增生(BPH),52例前列腺癌(PCa)及2例正常前列腺组织中5种miRNAs的表达情况。结果(1)miR-96、miR-183、miR-139在PCa中的表达率分别为67.31%(35/52),71.15%(37/52),67.31%(3s/52),分别与它们在BPH中的表达率44.74%(17/38),47.37%(18/38),39.47%(15/38)相比,差异有统计学意义(P〈0.05);miR-145在PCa中的表达率为21.15%(11/52),较BPH的63.16%(24/38)低(P〈o.01);miR-let-7d在PCa中的表达率为25.00%(13/52),较BPH的34.21%(13/38)无显著性差异(P〉0.05)。(2)5种miRNAs的表达与前列腺癌患者的年龄及血清PSA水平均无明显相关性(P〉0.05);5种miRNAs表达与肿瘤的G1eason评分均相关(P〈0.01);miR-96、miR-let-7d及miR-139的表达与肿瘤的临床分期有关(P〈0.01),miR-145、miR-183的表达则与其无明显相关性(P〉0.05);miR-96的表达与肿瘤累及前列腺的叶数相关(P〈0.01),而其余四种miRNAs的表达则与其无明显相关性(P〉0.05)。结论miRNAs与前列腺癌的发生有关,并可能作为前列腺癌早期诊断及预后评估的重要生物标记物。 相似文献
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微小RNA(miRNA)是一类22核苷酸的高度保守的小分子非编码RNA,主要通过与靶mRNA的3′非编码区碱基互补配对结合而引起靶RNA降解或翻译抑制.越来越多的研究显示,miRNA在免疫反应中具有新型调节作用,包括调节免疫细胞的发育和分化、B细胞抗体的产生、炎性介质的释放、细胞信号转导等.miRNA在维持机体免疫系统... 相似文献
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Yan Lv Cheng Lu Xiaohong Ji Zhijing Miao Wei Long Hongjuan Ding Mingming Lv 《Journal of cellular physiology》2019,234(2):1052-1061
Preeclampsia (PE) is a complex disorder that is characterized by hypertension and proteinuria after the 20th week of pregnancy, and it causes most neonatal morbidity and perinatal mortality. Most studies suggest that placental dysfunction is the main cause of PE. However, genetic factors, immune factors, and systemic inflammation are also related to the pathophysiology of this syndrome. Thus far, the exact pathogenesis of PE is not yet fully understood, and intense research efforts are focused on PE to elucidate the pathophysiological mechanisms. MicroRNAs (miRNAs) refer to small single-stranded and noncoding molecules that can negatively regulate gene expression, and miRNA regulatory networks play an important role in diverse pathological processes. Many studies have confirmed deregulated miRNA in pregnant patients with PE, and the function and mechanism of these differentially expressed miRNA are gradually being revealed. In this review, we summarize the current research about miRNA involved in PE, including placenta-specific miRNA, their predictive value, and their function in the development of PE. This review will provide fundamental evidence of miRNA in PE, and further studies are necessary to explore the roles of miRNA in the early diagnosis and treatment of PE. 相似文献
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microRNAs是一类非蛋白质编码小RNA,通常作用于靶基因mRNA的3′-UTR区引起靶基因的翻译抑制或降解。microRNAs的表达具有组织特异性,骨骼肌和心肌中有特异microRNAs的表达。microRNAs在肌肉的增殖、分化等发育过程中发挥重要的调节作用,并且microRNAs的表达异常与某些肌肉疾病的病理过程有关。现就microRNAs在肌肉中的作用研究进展作一综述。 相似文献
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HyunTaek Jung Jae Seok Kim Keum Hwa Lee Kalthoum Tizaoui Salvatore Terrazzino Sarah Cargnin Lee Smith Ai Koyanagi Louis Jacob Han Li Sung Hwi Hong Dong Keon Yon Seung Won Lee Min Seo Kim Paul Wasuwanich Wikrom Karnsakul Jae Il Shin Andreas Kronbichler 《International journal of biological sciences》2021,17(8):2112
Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract that mainly affects young people. IBD is associated with various gastrointestinal symptoms, and thus, affects the quality of life of patients. Currently, the pathogenesis of IBD is poorly understood. Although intestinal bacteria and host immune response are thought to be major factors in its pathogenesis, a sufficient explanation of their role in its pathophysiologic mechanism has not been presented. MicroRNAs (miRNAs), which are small RNA molecules that regulate gene expression, have gained attention as they are known to participate in the molecular interactions of IBD. Recent studies have confirmed the important role of miRNAs in targeting certain molecules in signaling pathways that regulate the homeostasis of the intestinal barrier, inflammatory reactions, and autophagy of the intestinal epithelium. Several studies have identified the specific miRNAs associated with IBD from colon tissues or serum samples of IBD patients and have attempted to use them as useful diagnostic biomarkers. Furthermore, some studies have attempted to treat IBD through intracolonic administration of specific miRNAs in the form of nanoparticle. This review summarizes the latest findings on the role of miRNAs in the pathogenesis, diagnosis, and treatment of IBD. 相似文献
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心肌重塑是心脏在一些生理的或病理的刺激作用下,心肌细胞和心肌细胞外基质在细胞结构、功能、数量及遗传表型方面出现的明显的变化即心脏的大小、形状和功能的变化。心肌细胞和心肌细胞外基质从根本上参与了心肌重塑的过程。目前,对于影响心肌重塑的因素及作用机制的研究主要集中在血流动力学和神经体液方面。近年来,对于不良心肌重塑的逆转干预,包括药理干预、运动干预,一直持续不断,研究的不断深入给相关疾病的改善、治疗带了新的进展和希望。心肌重塑可能是生理性的或病理性的,生理性的重塑是心肌的适应性代偿性变化,而病理性的重塑是心肌的不适应变化,对身体产生危害性。本文主要对病理性心肌重塑的主要组成部分,影响心肌重塑的因素及相关机制,改善不良心肌重塑的有效干预做一个综述,并提出展望。 相似文献
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Paras K. Mishra Neetu Tyagi Soumi Kundu Suresh C. Tyagi 《Cell biochemistry and biophysics》2009,55(3):153-162
Elevated level of homocysteine (Hcy) called hyperhomocysteinemia (HHcy) is one of the major risk factors for chronic heart
failure. Although the role of Hcy in cardiac remodeling is documented, the regulatory mechanism involved therein is still
nebulous. MicroRNAs (miRNAs) and dicer have been implicated in regulation of cardiovascular diseases. Dicer is the only known
enzyme involved in miRNA maturation. We investigated the involvement of dicer and miRNA in Hcy-induced cardiac remodeling.
HL-1 cardiomyocytes were cultured in different doses of Hcy. Total RNA was isolated and RT-PCR and real-time PCR was performed
for dicer, MMP-2,-9, TIMP-1,-3, and NOX-4. MiRNA microarray was used for analyzing the differential expression of miRNAs.
Individual miRNA assay was also done. Western blotting was used to assess the MMP-9 expression in HHcy cardiomyocytes. The
RT-PCR results suggest that dicer expression is enhanced in HHcy cardiomyocytes suggesting its involvement in cardiac remodeling
caused due to high dose of Hcy. On the other hand, high dose of Hcy increased NOX-4 expression, a marker for oxidative stress.
Additionally, HHcy cardiomyocytes showed elevated levels of MMP-2,-9 and TIMP-1,-3, and reduced expression of TIMP-4, suggesting
cardiac remodeling due to oxidative stress. The miRNA microarray assay revealed differential expression of 11 miRNAs and among
them miR-188 show dramatic downregulation. These findings suggest that dicer and miRNAs especially miR-188 are involved in
Hcy-induced cardiac remodeling. 相似文献
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MicroRNA(miRNA)是一类小的非编码RNA,它们主要在转录后水平对靶mRNA进行切割或抑制mRNA的翻译来调控基因的表达. miRNA通过对靶基因的调控参与植物的生长发育、胁迫应答和代谢过程.在水稻中,已经发现并初步验证了许多与生长发育相关的miRNA,它们对水稻不同器官和形态发育发挥着重要作用.本文综述了水稻miRNA的发生和调控机制、靶基因的预测,重点介绍了miRNA对水稻生长发育和形态建成的研究进展,并对研究过程中存在的问题进行了讨论.为更好地了解miRNA及其靶基因在提高水稻产量和品质方面的作用,进一步解析miRNA在水稻中的调控机制提供参考. 相似文献