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Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression 总被引:1,自引:0,他引:1
Lei Li Xiaofei Yu Addie Thompson Michelle Guo Shigeo Yoshida Tadao Asami Joanne Chory Yanhai Yin 《The Plant journal : for cell and molecular biology》2009,58(2):275-286
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Masaya Ikezaki Mikiko Kojima Hitoshi Sakakibara Shoko Kojima Yoshihisa Ueno Chiyoko Machida Yasunori Machida 《The Plant journal : for cell and molecular biology》2010,61(1):70-82
The asymmetric leaves 1 ( as1 ) and as2 mutants of Arabidopsis thaliana exhibit pleiotropic phenotypes. Expression of a number of genes, including three class-1 KNOTTED -like homeobox ( KNOX ) genes ( BP , KNAT2 and KNAT6 ) and ETTIN / ARF3 , is enhanced in these mutants. In the present study, we attempted to identify the phenotypic features of as1 and as2 mutants that were generated by ectopic expression of KNOX genes, using multiple loss-of-function mutations of KNOX genes as well as as1 and as2 . Our results revealed that the ectopic expression of class-1 KNOX genes resulted in reductions in the sizes of leaves, reductions in the size of sepals and petals, the formation of a less prominent midvein, the repression of adventitious root formation and late flowering. Our results also revealed that the reduction in leaf size and late flowering were caused by the repression, by KNOX genes, of a gibberellin (GA) pathway in as1 and as2 plants. The formation of a less prominent midvein and the repression of adventitious root formation were not, however, related to the GA pathway. The asymmetric formation of leaf lobes, the lower complexity of higher-ordered veins, and the elevated frequency of adventitious shoot formation on leaves of as1 and as2 plants were not rescued by multiple mutations in KNOX genes. These features must, therefore, be controlled by other genes in which expression is enhanced in the as1 and as2 mutants. 相似文献
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Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems 总被引:20,自引:0,他引:20
Maruyama K Sakuma Y Kasuga M Ito Y Seki M Goda H Shimada Y Yoshida S Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2004,38(6):982-993
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The transcriptional repressor REST is a critical regulator of the neurosecretory phenotype 总被引:1,自引:0,他引:1
Bruce AW Krejcí A Ooi L Deuchars J Wood IC Dolezal V Buckley NJ 《Journal of neurochemistry》2006,98(6):1828-1840
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A single amino acid substitution in the R3 domain of GLABRA1 leads to inhibition of trichome formation in Arabidopsis without affecting its interaction with GLABRA3 下载免费PDF全文
Xuemei Dai Limei Zhou Wei Zhang Ling Cai Hongyan Guo Hainan Tian John Schiefelbein Shucai Wang 《Plant, cell & environment》2016,39(4):897-907
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The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors 总被引:25,自引:0,他引:25
Zoe A. Wilson Shaun M. Morroll Janet Dawson Ranjan Swarup Paddy J. Tighe 《The Plant journal : for cell and molecular biology》2001,28(1):27-39
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The Ca2+/calmodulin2‐binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in Arabidopsis 下载免费PDF全文
Huihui Fang Zhiqiang Liu Yanping Long Yali Liang Zhuping Jin Liping Zhang Danmei Liu Hua Li Jixian Zhai Yanxi Pei 《The Plant journal : for cell and molecular biology》2017,91(6):1038-1050
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Lilan Luo Sayuri Ando Yuki Sakamoto Takanori Suzuki Hiro Takahashi Nanako Ishibashi Shoko Kojima Daisuke Kurihara Tetsuya Higashiyama Kotaro T. Yamamoto Sachihiro Matsunaga Chiyoko Machida Michiko Sasabe Yasunori Machida 《The Plant journal : for cell and molecular biology》2020,101(5):1118-1134
In Arabidopsis, the ASYMMETRIC LEAVES2 (AS2) protein plays a key role in the formation of flat symmetric leaves via direct repression of the abaxial gene ETT/ARF3. AS2 encodes a plant‐specific nuclear protein that contains the AS2/LOB domain, which includes a z inc‐f inger (ZF) motif that is conserved in the AS2/LOB family. We have shown that AS2 binds to the coding DNA of ETT/ARF3, which requires the ZF motif. AS2 is co‐localized with AS1 in perinucleolar bodies (AS2 bodies). To identify the amino acid signals in AS2 required for formation of AS2 bodies and function(s) in leaf formation, we constructed recombinant DNAs that encoded mutant AS2 proteins fused to yellow fluorescent protein. We examined the subcellular localization of these proteins in cells of cotyledons and leaf primordia of transgenic plants and cultured cells. The amino acid signals essential for formation of AS2 bodies were located within and adjacent to the ZF motif. Mutant AS2 that failed to form AS2 bodies also failed to rescue the as2‐1 mutation. Our results suggest the importance of the formation of AS2 bodies and the nature of interactions of AS2 with its target DNA and nucleolar factors including NUCLEOLIN1. The partial overlap of AS2 bodies with perinucleolar chromocenters with condensed ribosomal RNA genes implies a correlation between AS2 bodies and the chromatin state. Patterns of AS2 bodies in cells during interphase and mitosis in leaf primordia were distinct from those in cultured cells, suggesting that the formation and distribution of AS2 bodies are developmentally modulated in plants. 相似文献
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Sara Luz Morales‐Lázaro Ricardo González‐Ramírez Pablo Gómez Victor Tapia‐Ramírez Mario Bermúdez De León Bulmaro Cisneros 《Journal of neurochemistry》2010,112(2):474-485
In this study, we delineated the molecular mechanisms that modulate Dp71 expression during neuronal differentiation, using the N1E‐115 cell line. We demonstrated that Dp71 expression is up‐regulated in response to cAMP‐mediated neuronal differentiation of these cells, and that this induction is controlled at promoter level. Functional deletion analysis of the Dp71 promoter revealed that a 5′‐flanking 159‐bp DNA fragment that contains Sp1 and AP2 binding sites is necessary and sufficient for basal expression of this TATA‐less promoter, as well as for its induction during neuronal differentiation. Electrophoretic mobility shift and chromatin immunoprecipitation assays revealed that Sp1 and AP2α bind to their respective DNA elements within the Dp71 basal promoter. Overall, mutagenesis assays on the Sp1 and AP2 binding sites, over‐expression of Sp1 and AP2α, as well as knock‐down experiments on Sp1 and AP2α gene expression established that Dp71 basal expression is controlled by the combined action of Sp1 and AP2α, which act as activator and repressor, respectively. Furthermore, we demonstrated that induction of Dp71 expression in differentiated cells is the result of the maintenance of positive regulation exerted by Sp1, as well as of the loss of AP2α binding, which ultimately releases the promoter from repression. 相似文献
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Hershko DD Robb BW Luo GJ Hungness ES Hasselgren PO 《Journal of cellular biochemistry》2003,90(3):627-640
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