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1.
The temperature course in the lateral semicircular canal and in the facial canal was studied in experiments during freezing of the semicircular canal. The course of the temperature was measured with thermocouples. Concurrently, the heat flow was measured, and also the total heat exchange was measured throughout the freezing period by a thermoelectric heat flowmeter incorporated in the cryotip. The measurements showed correlation between the total amount of heat exchanged, the freezing time, and the temperature in the semicircular canal. This correlation was utilized to assess and calculate (the temperature of the lateral semicircular canal) the course of the cryoprocess in vivo, where it is possible to measure the heat flow and the total heat exchange during the freezing period only. 2. Results upon Vertigo | No Vertigo | Improved | Unchanged | Number of patients | 7 | 5 | 3 | 相似文献
2.
High pressure (above 238 atmg) substantially extends the refrigerated storage of highly perishable biological material in a nonfrozen state. 5. Calculated Equivalent Values of Pressure and Temperature for Reducing Reaction Rates of Horseradish Peroxidase Pressure (atmg) | Temperature (°K) | a | 0 | 296 | 0.999 | 272 | 289 | 0.975 | 340 | 287 | 0.969 | 408 | 285 | 0.963 |
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3.
Measurements of the reproducibility of a random selection of copper/constantan thermocouples were made and it was found that they agreed within 1 ° C. Based on this finding, a digital thermocouple thermometer was designed and constructed incorporating a thermocouple linearizer and cold junction compensation. The instrument Accuracy of the Completed Digital Thermometer Temperature | Indicated | Error | (°C) | temperature | (°C) | | (°C) | | ?1.95.75 | ?195 | 0.75 | ?77.02 | ?78 | 0.38 | 0 | 0 | 0 | 52.49 | 53 | 0.51 | Mean | | 0.413 | 相似文献
4.
Correlated studies on volume distributions and cation (Na + and K +) content of CHO cells in suspension were carried out after various exposures to hypertonic NaCl or sucrose (500–7550 m Osm in both the presence and absence of DMSO (5–20%; ). The effects superimposed by ouabain (10 ?2–10 ?4m), amphotericin B (6–18 μg/ml), and glutaraldehyde (1.25%) on the above-mentioned parameters were also investigated. Volumetric analysis of CHO cells with the Coulter Channelyzer indicated a biphasic dose-dependent response to hypertonic media, the duration of the TABLE 2. Correlation between Volume, Survival, and Cation Content of CHO Cells Exposed to Hypertonic Media in Suspension Osmolality mOsm | Exposure (min) | | | | | | | | Hypertonic agent | | | | | | NaCl | | | | Sucrose | | | | Na+ | K+ | | V | Na+ | K+ | S | 1000 | 60 or less | Small | High | High | High | Normal | Low | High | High | 1500–2000 | 60 or less | Small | High | Low | Low | Normal | Low | High | High | 2000 or over | 60 or more | Small or largec | High | Very low | Very low | Small | Very low | Very low | Very low | 相似文献
5.
The activity levels of DNA polymerases α and β have been measured by autoradiography in squash preparations from rat testis of sexually mature animals. Similar results were obtained with ‘fixed’ samples (dipped in acetone: ethanol for 5 min at 25 °C) or ‘unfixed’ samples (frozen in liquid nitrogen and freeze-dried). The activities of DNA polymerases α and β in situ were distinguished by differential assay conditions and by selective inhibition with compounds such as N-ethylmaleimide and aphidicolin. Using the endogenous chromatin as template, maximal activity for both enzymes was obtained in the presence of all four deoxyribonucleoside triphosphates, MgCl 2 and ethylene glycol. When DNA polymerase activities in several predominant testicular cell types (pre-leptotene primary spermatocytes, pachytene primary spermatocytes, round spermatids and elongated spermatids) were quantitatively compared, on a per cell basis, the following percentage distribution was observed: | Pre-leptotene primary spermatocyte % | Pachytene primary spermatocyte % | Round spermatid % | Elongated spermatid % | DNA polymerase α | 25 | 42 | 30 | 3 | DNA polymerase β | 29 | 34 | 36 | 1 | 相似文献
6.
We have studied the integrity of lysosomes in isolated rat livers perfused for 3, 4, or 6 hr at 35 °C with BSA (40 g/l) in Krebs Ringer bicarbonate buffer. The latency and sedimentability of β-glucuronidase in homogenates of these livers was well maintained even after 6 hr. The latency and sedimentability of acid phosphatase remained at about control levels during the first 4 hr of perfusion but decreased between 4 and 6 hr. These decreases in latency and sedimentability correlated with a decrease in bile production and an increase in the rate of release of GOT into the perfusate and could indicate either intracellular disruption of lysosomes Latencies, Sedimentabilities, and Specific Activities of Acid Phosphatase and β-Glucuronidase in Homogenates of Rat Liver Prepared before or at Various Times after Exposure to 1.4 m Me2SO for 1 hr Time (hr) | 0 | 3 | 4 | 6 | Acid phosphatase | | | | | Latency (%) | 83.2 ± 0.8 | 64.7 ± 5.1 | 62.4 ± 7.9 | 68.9 ± 5.4 | Sedimentability (%) | 81.7 ± 0.6 | 77.6 ± 3.1 | 81.0 ± 3.4 | 79.4 ± 5.1 | Specific activity (mIU/mg protein) | 2.8 ± 0.4 | 2.8 ± 0.2 | 2.4 ± 0.4 | 1.6 ± 0.2 | β-Glucuronidase | | | | | Latency (%) | 06.8 ± 1.5 | 71.3 ± 4.3 | 74.2 ± 2.5 | 63.3 ± 4.5 | Sedimentability (%) | 69.5 ± 0.3 | 75.4 ± 3.2 | 75.0 ± 1.1 | 74.6 ± 2.0 | Specific activity (mIU/mg protein) | 1.8 ± 0.1 | 1.6 ± 0.1 | 1.6 ± 0.2 | 1.6 ± 0.2 | 相似文献
7.
A commercially available tissue culture medium has been proven capable of preserving dog kidney function for at least 24 hr after simple cooling. The advantages of using tissue culture medium as preservation fluid instead of plasma or albumin solutions from the infectious and immunological points of view are obvious. An in vitro study was completed using the tissue 1. Code (animal No.) | Perfusion time (br) | Perfusion pressure mm/Hg | Flow ml/min | Weight gain | pH | pO2 mm/Hg | Histological appearance | 1 | 24 | 70-60 systolic | 96 | 35 | 7.3 | 150–180 | Grossly normal | 2 | 24 | | 45-40 diastolic | 108 | 30 | | | 3 | 24 | | 96 | 30 | | | | 4 | 48 | 70-60 systolic | 80 | 35 | 7.3 | 150–180 | Grossly normal | 5 | 48 | 45-40 diastolic | 120 | 40 | 7.4 | | | 6 | 48 | | 100 | 40 | | | | 7 | 72 | 70-60 systolic | 115 | 40 | 7.4 | 150–180 | Slight vacuolization of the tubular cells | 8 | 72 | 45-40 diastolic | 96 | 40 | | | | 9 | 72 | | 80 | 40 | | | | 10 | 24 | 70-60 systolic | 110 | 35 | 7.3 | 150–180 | Used for transplantation | 11 | 24 | 45-40 diastolic | 120 | 35 | | | | 12 | 24 | | 140 | 40 | | | | 13 | 24 | | 100 | 30 | | | | 14 | 24 | | 96 | 30 | | | | 相似文献
8.
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology. Contents (chosen by) | 525 | Cytoskeleton (Desai and Holleran) | 526 | Cell regulation (Roche, Servant and Weiner) | 528 | Nucleus and gene expression (Aasland and Weinzierl) | 529 | Membranes and sorting (Ponnambalam) | 530 | Membrane permeability (Slesinger) | 531 | Cell-to-cell contact and extracellular matrix (Pfaff) | 533 | Cell differentiation (van Roessel, Kaltschmidt, Tsang and Huckriede) | 534 | Cell multiplication (Sclafani) | 相似文献
9.
Five ewes of each of four breed types were kept in each of two environmentally-controlled rooms over a period of 2.5 years. The daylength varied between 20 and 9 h on a 6-monthly cycle, including a period of 22 weeks during which daylength was decreased by 0.5 h per week, 2 weeks in which it increased from 9 to 20 h, and 2 weeks at 20 h; each room operated 3 months out of phase with the other. Towards the end of the period of decreasing daylength each ewe was synchronised with a progestagen sponge for 12 days, given an injection (i.m.) of 750 I.U. pregnant mares' serum gonadotrophin (PMSG) on withdrawal, and inseminated 48 and 58 h later (500 million fresh undiluted sperm per insemination). Thus, insemination occurred at a single synchronised oestrus every 6 months. Progesterone pregnancy diagnosis was performed on blood samples taken 18 days later and parturition was induced by an injection (i.m.) of 16 mg dexamethasone on day 143 of gestation. Lambs were weaned at birth, allowing the ewes approximately 5.5 weeks to recover before the next insemination and a ‘successive’ conception.The performance of the four types of ewe, starting as maidens, is tabulated. Conceptions | Merino | Dorset Horn × Merino | Border Leic. × Merino | South Suffolk × Merino | | Successive (%) | 20.0 | 12.5 | 11.1 | 47.6 | | Non-successive (%) | 76.0 | 76.9 | 66.7 | 95.0 | | Overall (%) | 51.1 | 52.4 | 44.4 | 70.7 | | Mean litter size | 1.7 | 1.5 | 1.5 | 2.2 | | Mean lambs per ewe per year | 1.8 | 1.6 | 1.3 | 3.1 | | 相似文献
10.
Glaucomatocyclitic crisis should be considered in the differential diagnosis of unilaterally increased IOP. A careful history, slit-lamp examination and gonioscopic examination will assist the examiner in making an accurate diagnosis.The etiology of this syndrome remains unknown. If indeed it is a disturbance of the anterior intraocular vasculature secondary to an autonomic abnormality, any of the proposed etiologies might possibly trigger an attack (Table 2).Treatment at this time should consist of 1 gH. 0.5% apraclonidine given in office or a topical steroid, cycloplegic, and ocular hypotensive agent combination. Follow-up should be within 24 h to monitor for IOP reduction. If treatment with apraclonidine is initiated, additional drops should be instilled as needed to lower the IOP to acceptable levels. Prophylactic treatment with topical steroids has been considered in this mostly benign syndrome [14]. However, the potential side-effects of long-term steroid use and the unpredictable frequency of glaucomatocyclitic crises may preclude steroid use in these cases. Table 2. Proposed etiological factors and diseases related to glaucomatocyclitis crisis. Form of developmental glaucoma | Autonomic imbalance | Allergy, peptic ulcer, stress | Herpes simplex virus | Cytomegalovirus | Varicella-zoster virus | Mesodermal dysgenesis | 相似文献
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