共查询到20条相似文献，搜索用时 15 毫秒
1.
Xu Q Schwarzenbacher R McMullan D Abdubek P Ambing E Biorac T Canaves JM Chiu HJ Dai X Deacon AM DiDonato M Elsliger MA Godzik A Grittini C Grzechnik SK Hampton E Hornsby M Jaroszewski L Klock HE Koesema E Kreusch A Kuhn P Lesley SA Levin I Miller MD Morse A Moy K Ouyang J Page R Quijano K Reyes R Robb A Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J von Delft F Wang X West B White A Wolf G Zagnitko O Hodgson KO Wooley J Wilson IA 《Proteins》2005,58(4):976-981
2.
Bakolitsa C Schwarzenbacher R McMullan D Brinen LS Canaves JM Dai X Deacon AM Elsliger MA Eshagi S Floyd R Godzik A Grittini C Grzechnik SK Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Quijano K Robb A Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J von Delft F Wang X West B Wolf G Hodgson KO Wooley J Wilson IA 《Proteins》2004,56(3):607-610
3.
Rife C Schwarzenbacher R McMullan D Abdubek P Ambing E Axelrod H Biorac T Canaves JM Chiu HJ Deacon AM DiDonato M Elsliger MA Godzik A Grittini C Grzechnik SK Hale J Hampton E Han GW Haugen J Hornsby M Jaroszewski L Klock HE Koesema E Kreusch A Kuhn P Lesley SA Miller MD Moy K Nigoghossian E Paulsen J Quijano K Reyes R Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J White A Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2005,61(2):444-448
4.
Levin I Miller MD Schwarzenbacher R McMullan D Abdubek P Ambing E Biorac T Cambell J Canaves JM Chiu HJ Deacon AM DiDonato M Elsliger MA Godzik A Grittini C Grzechnik SK Hale J Hampton E Han GW Haugen J Hornsby M Jaroszewski L Karlak C Klock HE Koesema E Kreusch A Kuhn P Lesley SA Morse A Moy K Nigoghossian E Ouyang J Page R Quijano K Reyes R Robb A Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Xu Q Zagnitko O Hodgson KO Wooley J Wilson IA 《Proteins》2005,59(4):864-868
5.
Page R Nelson MS von Delft F Elsliger MA Canaves JM Brinen LS Dai X Deacon AM Floyd R Godzik A Grittini C Grzechnik SK Jaroszewski L Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McMullan D McPhillips TM Miller MD Morse A Moy K Ouyang J Robb A Rodrigues K Schwarzenbacher R Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Hodgson KO Wooley J Wilson IA 《Proteins》2004,54(1):157-161
6.
The bacterial enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) catalyzes the unprecedented transfer and isomerization of the ribosyl moiety of S-adenosylmethionine (AdoMet) to a modified tRNA nucleoside in the biosynthesis of the hypermodified nucleoside queuosine. The complexity of this reaction makes it a compelling problem in fundamental mechanistic enzymology, and as part of our mechanistic studies of the QueA-catalyzed reaction, we report here the elucidation of the steady-state kinetic mechanism. Bi-substrate kinetic analysis gave initial velocity patterns indicating a sequential mechanism, and provided the following kinetic constants: K (M)(tRNA)= 1.9 +/- 0.7 microM and K (M)(AdoMet)= 98 +/- 5.0 microM. Dead-end inhibition studies with the substrate analogues S-adenosylhomocysteine and sinefungin gave competitive inhibition patterns against AdoMet and noncompetitive patterns against preQ(1)-tRNA(Tyr), with K(i) values of 133 +/- 18 and 4.6 +/- 0.5 microM for sinefungin and S-adenosylhomocysteine, respectively. Product inhibition by adenine was noncompetitive against both substrates under conditions with a subsaturating cosubstrate concentration and uncompetitive against preQ(1)-tRNA(Tyr) when AdoMet was saturating. Inhibition by the tRNA product (oQ-tRNA(Tyr)) was competitive and noncompetitive against the substrates preQ(1)-tRNA(Tyr) and AdoMet, respectively. Inhibition by methionine was uncompetitive versus preQ(1)-tRNA(Tyr), but noncompetitive against AdoMet. However, when methionine inhibition was investigated at high AdoMet concentrations, the pattern was uncompetitive. Taken together, the data are consistent with a fully ordered sequential bi-ter kinetic mechanism in which preQ(1)-tRNA(Tyr) binds first followed by AdoMet, with product release in the order adenine, methionine, and oQ-tRNA. The chemical mechanism that we previously proposed for the QueA-catalyzed reaction [Daoud Kinzie, S., Thern, B., and Iwata-Reuyl, D. (2000) Org. Lett. 2, 1307-1310] is consistent with the constraints imposed by the kinetic mechanism determined here, and we suggest that the magnitude of the inhibition constants for the dead-end inhibitors may provide insight into the catalytic strategy employed by the enzyme. 相似文献
7.
Grimm C Ficner R Sgraja T Haebel P Klebe G Reuter K 《Biochemical and biophysical research communications》2006,351(3):695-701
The enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) is involved in the biosynthesis of the hypermodified tRNA nucleoside queuosine. It is unprecedented in nature as it uses the cofactor S-adenosylmethionine as the donor of a ribosyl group. We have determined the crystal structure of Bacillus subtilis QueA at a resolution of 2.9A. The structure reveals two domains representing a 6-stranded beta-barrel and an alpha beta alpha-sandwich, respectively. All amino acid residues invariant in the QueA enzymes of known sequence cluster at the interface of the two domains indicating the localization of the substrate binding region and active center. Comparison of the B. subtilis QueA structure with the structure of QueA from Thermotoga maritima suggests a high domain flexibility of this enzyme. 相似文献
8.
Jin KK Krishna SS Schwarzenbacher R McMullan D Abdubek P Agarwalla S Ambing E Axelrod H Canaves JM Chiu HJ Deacon AM DiDonato M Elsliger MA Feuerhelm J Godzik A Grittini C Grzechnik SK Hale J Hampton E Haugen J Hornsby M Jaroszewski L Klock HE Knuth MW Koesema E Kreusch A Kuhn P Lesley SA Miller MD Moy K Nigoghossian E Okach L Oommachen S Paulsen J Quijano K Reyes R Rife C Stevens RC Spraggon G van den Bedem H Velasquez J White A Wolf G Han GW Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2006,63(4):1112-1118
9.
Spraggon G Schwarzenbacher R Kreusch A McMullan D Brinen LS Canaves JM Dai X Deacon AM Elsliger MA Eshagi S Floyd R Godzik A Grittini C Grzechnik SK Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kuhn P McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Quijano K Rezezadeh F Robb A Sims E Stevens RC van den Bedem H Velasquez J Vincent J von Delft F Wang X West B Wolf G Xu Q Hodgson KO Wooley J Lesley SA Wilson IA 《Proteins》2004,56(2):396-400
10.
Crystal structure of a transcription regulator (TM1602) from Thermotoga maritima at 2.3 A resolution
Weekes D Miller MD Krishna SS McMullan D McPhillips TM Acosta C Canaves JM Elsliger MA Floyd R Grzechnik SK Jaroszewski L Klock HE Koesema E Kovarik JS Kreusch A Morse AT Quijano K Spraggon G van den Bedem H Wolf G Hodgson KO Wooley J Deacon AM Godzik A Lesley SA Wilson IA 《Proteins》2007,67(1):247-252
11.
Schwarzenbacher R Canaves JM Brinen LS Dai X Deacon AM Elsliger MA Eshaghi S Floyd R Godzik A Grittini C Grzechnik SK Guda C Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McMullan D McPhillips TM Miller MA Miller MD Morse A Moy K Ouyang J Robb A Rodrigues K Selby TL Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Hodgson KO Wooley J Wilson IA 《Proteins》2003,53(1):142-145
12.
Heine A Canaves JM von Delft F Brinen LS Dai X Deacon AM Elsliger MA Eshaghi S Floyd R Godzik A Grittini C Grzechnik SK Guda C Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McMullan D McPhillips TM Miller MA Miller MD Morse A Moy K Ouyang J Page R Robb A Rodrigues K Schwarzenbacher R Selby TL Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Hodgson KO Wooley J Wilson IA 《Proteins》2004,56(2):387-391
13.
Mathews II Krishna SS Schwarzenbacher R McMullan D Abdubek P Ambing E Canaves JM Chiu HJ Deacon AM DiDonato M Elsliger MA Godzik A Grittini C Grzechnik SK Hale J Hampton E Han GW Haugen J Jaroszewski L Klock HE Koesema E Kreusch A Kuhn P Lesley SA Levin I Miller MD Moy K Nigoghossian E Paulsen J Quijano K Reyes R Spraggon G Stevens RC van den Bedem H Velasquez J White A Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2006,63(4):1106-1111
14.
Jaroszewski L Schwarzenbacher R von Delft F McMullan D Brinen LS Canaves JM Dai X Deacon AM DiDonato M Elsliger MA Eshagi S Floyd R Godzik A Grittini C Grzechnik SK Hampton E Levin I Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Quijano K Reyes R Rezezadeh F Robb A Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2004,56(3):611-614
15.
Xu Q Krishna SS McMullan D Schwarzenbacher R Miller MD Abdubek P Agarwalla S Ambing E Astakhova T Axelrod HL Canaves JM Carlton D Chiu HJ Clayton T DiDonato M Duan L Elsliger MA Feuerhelm J Grzechnik SK Hale J Hampton E Han GW Haugen J Jaroszewski L Jin KK Klock HE Knuth MW Koesema E Kreusch A Kuhn P Morse AT Nigoghossian E Okach L Oommachen S Paulsen J Quijano K Reyes R Rife CL Spraggon G Stevens RC van den Bedem H White A Wolf G Hodgson KO Wooley J Deacon AM Godzik A Lesley SA Wilson IA 《Proteins》2006,65(3):777-782
16.
DiDonato M Krishna SS Schwarzenbacher R McMullan D Jaroszewski L Miller MD Abdubek P Agarwalla S Ambing E Axelrod H Biorac T Chiu HJ Deacon AM Elsliger MA Feuerhelm J Godzik A Grittini C Grzechnik SK Hale J Hampton E Haugen J Hornsby M Klock HE Knuth MW Koesema E Kreusch A Kuhn P Lesley SA Moy K Nigoghossian E Okach L Paulsen J Quijano K Reyes R Rife C Spraggon G Stevens RC van den Bedem H Velasquez J White A Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2006,63(1):256-260
17.
Schwarzenbacher R von Delft F Jaroszewski L Abdubek P Ambing E Biorac T Brinen LS Canaves JM Cambell J Chiu HJ Dai X Deacon AM DiDonato M Elsliger MA Eshagi S Floyd R Godzik A Grittini C Grzechnik SK Hampton E Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA Levin I McMullan D McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Quijano K Robb A Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2004,56(2):392-395
18.
Brinen LS Canaves JM Dai X Deacon AM Elsliger MA Eshaghi S Floyd R Godzik A Grittini C Grzechnik SK Guda C Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McMullan D McPhillips TM Miller MA Miller MD Morse A Moy K Ouyang J Robb A Rodrigues K Selby TL Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J Wang X West B Wolf G Taylor SS Hodgson KO Wooley J Wilson IA 《Proteins》2003,50(2):371-374
19.
Santelli E Schwarzenbacher R McMullan D Biorac T Brinen LS Canaves JM Cambell J Dai X Deacon AM Elsliger MA Eshagi S Floyd R Godzik A Grittini C Grzechnik SK Jaroszewski L Karlak C Klock HE Koesema E Kovarik JS Kreusch A Kuhn P Lesley SA McPhillips TM Miller MD Morse A Moy K Ouyang J Page R Quijano K Rezezadeh F Robb A Sims E Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J von Delft F Wang X West B Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2004,56(1):167-170
20.
Jaroszewski L Schwarzenbacher R McMullan D Abdubek P Agarwalla S Ambing E Axelrod H Biorac T Canaves JM Chiu HJ Deacon AM DiDonato M Elsliger MA Godzik A Grittini C Grzechnik SK Hale J Hampton E Han GW Haugen J Hornsby M Klock HE Koesema E Kreusch A Kuhn P Lesley SA Miller MD Moy K Nigoghossian E Paulsen J Quijano K Reyes R Rife C Spraggon G Stevens RC van den Bedem H Velasquez J Vincent J White A Wolf G Xu Q Hodgson KO Wooley J Wilson IA 《Proteins》2005,61(3):669-673