旧石器考古学的目标、制约和挑战

旧石器研究可以设法解决包括现代人起源和扩散在内的最重大的考古学问题,因此常常会引起公众的关注和想象。要重建旧石器时代人类生活状况显然也面临着很多问题,既有数据局限性导致的实践上的限制,也有试图理解发现本身时遇到的解释性的困难。过去的研究已经证明了实验考古学和民族考古学可以解决其中一些问题,但这也突显了考古学家所面临的限制。     

Perspectives on Global-Change Archaeology

In this article, I explore the characteristics of global-change archaeology as an emerging field of research. Global-change archaeology seeks to document and apply historical knowledge of past human–environmental interactions to the understanding of contemporary environmental problems and management and planning for future sustainability. It takes place within an interdisciplinary research structure and is situated within the explanatory contexts of historical science and humanistic history with close links to historical and political ecology. Both history and agency play important roles in the practice of global-change archaeology. Past human decision making in the context of cultural attitudes and perceptions also has a significant role in the archaeology of global change.     

Paleoethnobotany: Modern Research Connecting Ancient Plants and Ancient Peoples

Paleoethnobotany is a growing subdiscipline of archaeology that utilizes information from numerous other disciplines to show the relationships between ancient plants and ancient peoples. The two primary disciplines that underlie paleoethnobotanical research are archaeology and botany. As such, the results of ongoing botanical research on taphonomic processes, genetic identification of ancient plant types, pollen analysis, phytoliths analysis, and seed identification directly affect the strength of paleoethnobotanical models of past human behavior. Preserved seeds form a significant portion of the archaeobotanical record. They represent not only the environment that was present when they were deposited but also a connection to the activity and culture of ancient people. Using the type of archaeobotanical remains and the archeological context of the remains, paleoethnobotanists study a diverse range of topics. These topics include, but are not limited to, the use of plants in ancient cultures, the development and rise of agriculture, and the relationship between agriculture and settlement patterns.     

Biological Analogy, Diffusionism, and Archaeology

A "biologization" of anthropological theory has recently been proposed by scholars from various subfields of the discipline. One aspect of that phenomenon is Harold K. Schneider's proposal to rewrite world history as a diffusionary process based on a biological model. It is asserted that he fails for several reasons: a misunderstanding of biology, a misunderstanding of archaeology, and a rejection of empiricism, making his theory less testable than standard evolutionary theory. The role of diffusion in culture change is not explained by recourse to faulty analogy and highly selected archaeological data. [biological analogy, diffusionism, archaeological theory, cultural evolution]     

宾福德的周口店埋藏学研究与旧石器考古学理论建构

本文回顾了1985年美国考古学家路易斯·宾福德访华的背景和产生的影响,认为这次中美旧石器考古合作的失败原因是两国学者之间在认识论和范式上的巨大差异。宾福德对周口店的埋藏学思考是立足于过程考古学中程理论和他自己的埋藏学探索的基础之上,并体现在他的《纽纳缪特民族考古学》和《骨骼：古代人类与现代之谜》两本著作中。宾福德中程理论和参考框架建设为我们提供了一种启示和借鉴,有助于中国旧石器考古学努力构建和完善自己的考古学理论框架。这种框架需要重视材料的分析和归纳,提出研究的问题和解决的办法,然后根据埋藏学和动物考古学排除遗址形成过程中的自然改造因素,提炼人类行为的信息。最后从民族考古学类比的参考框架来达到重建文化历史、人类生存方式和文化变迁的三大目标。     

The role of the individual domains in the structure and function of the catalytic region of a modular serine protease, C1r.

The first enzymatic event in the classical pathway of complement activation is autoactivation of the C1r subcomponent of the C1 complex. Activated C1r then cleaves and activates zymogen C1s. C1r is a multidomain serine protease consisting of N-terminal alpha region interacting with other subcomponents and C-terminal gammaB region mediating proteolytic activity. The gammaB region consists of two complement control protein modules (CCP1, CCP2) and a serine protease domain (SP). To clarify the role of the individual domains in the structural and functional properties of the gammaB region we produced the CCP1-CCP2-SP (gammaB), the CCP2-SP, and the SP fragments in recombinant form in Escherichia coli. We successfully renatured the inclusion body proteins. After renaturation all three fragments were obtained in activated form and showed esterolytic activity on synthetic substrates similar to each other. To study the self-activation process in detail zymogen mutant forms of the three fragments were constructed and expressed. Our major statement is that the ability of autoactivation and C1s cleavage is an inherent property of the SP domain. We observed that the CCP2 module significantly increases proteolytic activity of the SP domain on natural substrate, C1s. Therefore, we propose that CCP2 module provides accessory binding sites. Differential scanning calorimetric measurements demonstrated that CCP2 domain greatly stabilizes the structure of SP domain. Deletion of CCP1 domain from the CCP1-CCP2-SP fragment results in the loss of the dimeric structure. Our experiments also provided evidence that dimerization of C1r is not a prerequisite for autoactivation.     

旧石器时代考古与栖居及生计形态分析

由于打制石器本身所含的人类行为信息比较有限,我国旧石器时代考古研究应该引入文化生态学的理论方法,从宏观的生态位、中观的遗址域和微观的居住面分析来充分提炼古人类觅食方式和资源利用的信息。本文介绍了栖居(聚落)形态和遗址域概念的方法论及意义,并用墨西哥瓦哈卡河谷圭拉那魁兹的研究案例来说明这些概念在人地关系研究和农业起源研究中的重要性。文章呼吁旧石器时代考古研究要从低端的分类描述转向人类环境和行为的重建,以期令我们的研究水准跻身世界先进水平。     

Settlement and landscape history at the Federsee,south-west Germany,as reflected in pollen diagrams

The Federsee mire in the Alpine Foreland of south-western Germany contains a record of a remarkable archaeological landscape. Since the first excavations in the 1920's, botanists and mire geologists have studied the relationship between landscape development and settlement at this site. In a new study, funded by the Deutsche Forschungsgemeinschaft, various disciplines embracing both archaeology and the natural sciences have come together to address outstanding questions and problems. Pollen analysis can only be carried out within the Federsee mire since no other suitable mires are found in the vicinity. Because of the size of the Federsee basin (30 km² at the end of the last glaciation), the regional pollen component, consisting predominantly of arboreal pollen, prevails over the herbaceous component which mainly reflects activity associated with settlements. Nevertheless, phases of settlement are clearly reflected in the radiocarbon-dated pollen diagrams and can be correlated with Neolithic and Bronze Age settlements that are dated either by dendrochronology or radiocarbon. In addition, some settlement phases were identified for which no archaeological evidence is yet available. As a consequence of human impact during the Atlantic and Subboreal periods, a gradual opening-up and change in structure of the forests is recorded. There is evidence for an exceptionally high level of human impact associated with two Bronze Age settlements that were present in the central part of the Federsee mire. Each of the five transgressions of the Federsee so far identified occurred at the end of a settlement phase. These may have resulted from anthropogenic activity rather than climatic change. A contribution to the 8th IPC, Aix-en-Provence, Sept. 1992     

Assembly and enzymatic properties of the catalytic domain of human complement protease C1r.

The catalytic properties of C1r, the protease that mediates activation of the C1 complex of complement, are mediated by its C-terminal region, comprising two complement control protein (CCP) modules followed by a serine protease (SP) domain. Baculovirus-mediated expression was used to produce fragments containing the SP domain and either 2 CCP modules (CCP1/2-SP) or only the second CCP module (CCP2-SP). In each case, the wild-type species and two mutants stabilized in the proenzyme form by mutations at the cleavage site (R446Q) or at the active site serine residue (S637A), were produced. Both wild-type fragments were recovered as two-chain, activated proteases, whereas all mutants retained a single-chain, proenzyme structure, providing the first experimental evidence that C1r activation is an autolytic process. As shown by sedimentation velocity analysis, all CCP1/2-SP fragments were dimers (5.5-5.6 S), and all CCP2-SP fragments were monomers (3.2-3.4 S). Thus, CCP1 is essential to the assembly of the dimer, but formation of a stable dimer is not a prerequisite for self-activation. Activation of the R446Q mutants could be achieved by extrinsic cleavage by thermolysin, which cleaved the CCP2-SP species more efficiently than the CCP1/2-SP species and yielded enzymes with C1s-cleaving activities similar to their active wild-type counterparts. C1r and its activated fragments all cleaved C1s, with relative efficiencies in the order C1r < CCP1/2-SP < CCP2-SP, indicating that CCP1 is not involved in C1s recognition.     

The Magdalenian Colonization of Southern Germany

Although the topics of migration and colonization have received renewed archaeological attention in recent years, their relevance to the deep past of hunter-gatherer archaeology has been debated. The Magdalenian colonization of southern Germany after the last glacial maximum, ca. 15,000-13,000 B.P., presents a case study in which many of the debated issues can be explored. Environmental change and relative demographic pressure played a causal role in population movements, leading to a gradual, discontinuous expansion from the Franco-cantabrian refugium. Active social strategies to overcome the risks facing frontier groups helped maintain remarkable uniformity in material culture across hundreds of kilometers, despite shifts in subsistence and settlement patterns required in the newly occupied areas, [archaeology. Palaeolithic, Europe, migration]     

Dancing The Past Into Life: The Rasa,Nrtta and Rāga of Immigrant Existence.

This paper attempts to explore the significant place that Indian ‘classical’ dance has held, both in postcolonial Indian nationalism, and in the middle‐class Indian diaspora's efforts to transmit the cultural past. While arguing that this orientation towards culture as a set of representations signals a fundamental breakdown in a more primary relation to the past, the paper turns to Indian dance and music for a language with which to appreciate both the full magical force of representations and the persistence of a level of embodied experience which is coherent and meaningful without being representational. If the past were available to us only in the form of express recollections, we should be continually tempted to recall it in order to verify its existence, and thus resemble the patient mentioned by Scheler, who was constantly turning round in order to reassure himself that things were really there—whereas in fact we feel it behind us as an incontestable acquisition. (Merleau‐Ponty, 1986:418)     

Crystal structure of Leishmania major peroxidase and characterization of the compound i tryptophan radical

The parasitic protozoa Leishmania major produces a peroxidase (L. major peroxidase; LmP) that exhibits activities characteristic of both yeast cytochrome c peroxidase (CCP) and plant cytosolic ascorbate peroxidase (APX). One common feature is a key Trp residue, Trp(208) in LmP and Trp(191) in CCP, that is situated adjacent to the proximal His heme ligand in CCP, APX, and LmP. In CCP, Trp(191) forms a stable cationic radical after reaction with H(2)O(2) to form Compound I; in APX, the radical is located on the porphyrin ring. In order to clarify the role of Trp(208) in LmP and to further probe peroxidase structure-function relationships, we have determined the crystal structure of LmP and have studied the role of Trp(208) using electron paramagnetic resonance spectroscopy (EPR), mutagenesis, and enzyme kinetics. Both CCP and LmP have an extended section of β structure near Trp(191) and Trp(208), respectively, which is absent in APX. This region provides stability to the Trp(191) radical in CCP. EPR of LmP Compound I exhibits an intense and stable signal similar to CCP Compound I. In the LmP W208F mutant, this signal disappears, indicating that Trp(208) forms a stable cationic radical. In LmP conversion of the Cys(197) to Thr significantly weakens the Compound I EPR signal and dramatically lowers enzyme activity. These results further support the view that modulation of the local electrostatic environment controls the stability of the Trp radical in peroxidases. Our results also suggest that the biological role of LmP is to function as a cytochrome c peroxidase.     

Archaeology, anthropology and subsistence

Wherever and whenever one may wish to place the roots of the disciplines of archaeology and anthropology, the subsistence-based categories of savage hunters and civilized farmers still lie at the heart of the division of much contemporary intellectual labour. The sources of these categories can be traced back into the seventeenth century, although they were first systematically related to (pre)history and cultural difference in the mid-eighteenth century. The subsequent relations between these categories and the changing disciplines of ethnology, ethnography, and archaeology have not remained constant over time or space. However, the underlying assumption that subsistence practices are meaningful and useful societal categories has persisted for the past 250 years. The relationship between such concepts, the closely associated idea of social evolution, and anthropology and archaeology, in particular from the mid-nineteenth century to the present, is examined. It is suggested that finding ways of writing across such categories is a necessary step for the future of both disciplines.     

Arable weed seeds as indicators of regional cereal provenance: a case study from Iron Age and Roman central-southern Britain

The ability to provenance crop remains from archaeological sites remains an outstanding research question in archaeology. Archaeobotanists have previously identified the movement of cereals on the basis of regional variations in the presence of cereal grain, chaff and weed seeds (the consumer–producer debate), and weed seeds indicative of certain soil types, principally at Danebury hillfort. Whilst the former approach has been heavily criticised over the last decade, the qualitative methods of the latter have not been evaluated. The first interregional trade in cereals in Britain is currently dated to the Iron Age hillfort societies of the mid 1st millennium bc. Several centuries later, the development of urban settlements in the Late Iron Age and Roman period resulted in populations reliant on food which was produced elsewhere. Using the case study of central-southern Britain, centred on the oppidum (large fortified settlement) and civitas capital of Silchester, this paper presents the first regional quantitative analysis of arable weed seeds in order to identify the origin of the cereals consumed there. Analysis of the weed seeds which were present with the fine sieve by-products of the glume wheat Triticum spelta (spelt) shows that the weed floras of samples from diverse geological areas can be separated on the basis of the soil requirements of individual taxa. A preliminary finding is that, rather than being supplied with cereals from the wider landscape of the chalk region of the Hampshire Downs, the crops were grown close to Late Iron Age Silchester. The method presented here requires further high quality samples to evaluate this conclusion and other instances of cereal movement in the past.     

Archaeology, Anthropology, and the Culture Concept

The culture concept has been central to anthropology since the formational period of the discipline. Yet for much of the discipline's history it was used without explicit definition. Recent attempts to define it have yielded a range of varied formulations in the subdisciplines of archaeology and sociocultural anthropology. Does this mean that the center of anthropology—shared belief in a unified culture concept—has been destroyed? Quite the opposite, the author concludes—the debate has yielded benefits.     

Engineering ascorbate peroxidase activity into cytochrome c peroxidase

Cytochrome c peroxidase (CCP) and ascorbate peroxidase (APX) have very similar structures, and yet neither CCP nor APX exhibits each other's activities with respect to reducing substrates. APX has a unique substrate binding site near the heme propionates where ascorbate H-bonds with a surface Arg and one heme propionate (Sharp et al. (2003) Nat. Struct. Biol. 10, 303-307). The corresponding region in CCP has a much longer surface loop, and the critical Arg residue that is required for ascorbate binding in APX is Asn in CCP. In order to convert CCP into an APX, the ascorbate-binding loop and critical arginine were engineered into CCP to give the CCP2APX mutant. The mutant crystal structure shows that the engineered site is nearly identical to that found in APX. While wild-type CCP shows no APX activity, CCP2APX catalyzes the peroxidation of ascorbate at a rate of approximately 12 min (-1), indicating that the engineered ascorbate-binding loop can bind ascorbate.     

The crystal structure of the zymogen catalytic domain of complement protease C1r reveals that a disruptive mechanical stress is required to trigger activation of the C1 complex

C1r is the modular serine protease (SP) that mediates autolytic activation of C1, the macromolecular complex that triggers the classical pathway of complement. The crystal structure of a mutated, proenzyme form of the catalytic domain of human C1r, comprising the first and second complement control protein modules (CCP1, CCP2) and the SP domain has been solved and refined to 2.9 A resolution. The domain associates as a homodimer with an elongated head-to-tail structure featuring a central opening and involving interactions between the CCP1 module of one monomer and the SP domain of its counterpart. Consequently, the catalytic site of one monomer and the cleavage site of the other are located at opposite ends of the dimer. The structure reveals unusual features in the SP domain and provides strong support for the hypothesis that C1r activation in C1 is triggered by a mechanical stress caused by target recognition that disrupts the CCP1-SP interfaces and allows formation of transient states involving important conformational changes.     

Art and the Re-Presentation of the Past

This article considers conceptual links between producing installation art works in the present and interpreting prehistoric lifeworlds. We consider connections between the work of contemporary 'landscape', 'environmental' or 'ecological' artists and an on-going landscape archaeology project centred on Leskernick Hill, Bodmin Moor in the south-west of Britain. We argue that the production of art works in the present can be a powerful means of interpreting the past in the present. Both the practices of interpreting the past and producing art result in the production of something new that transforms our understanding of place and space resulting in the creation of new meaning. Art and archaeology can act together dialectically to produce a novel conceptualization of the past and produce a means of relating to the past that is considerably more than the sum of its parts.     

Culture change and epidemiological patterns among the Hagahai,Papua New Guinea

The role of introduced epidemic disease in highland New Guinea is considered in light of recent debate concerning pre-contact adaptations. Seroepidemiological studies of the Hagahai, a small isolated group of hunterhorticulturalists in the fringe highlands of Papua New Guinea, document the recent introduction of mumps, hepatitis B, specific types of influenza, and rotavirus. Results are related to ethnographic findings, detailing past levels of intergroup contact and recent changes in settlement patterns, travel, feasting, health care, and other cultural factors. Data suggest that intergroup disease transmission is greatly increased decades before officially recorded time of contact and that mortality levels documented soon thereafter are not indicative of the pre-contact adaptation.     

Three-dimensional structure of a complement control protein module in solution.

The complement control protein (CCP) modules (also known as short consensus repeats) are defined by a consensus sequence within a stretch of about 60 amino acid residues. These modules have been identified more than 140 times in over 20 proteins, including 12 proteins of the complement system. The solution structure of the 16th CCP module from human complement factor H has been determined by a combination of 2-dimensional nuclear magnetic resonance spectroscopy and restrained simulated annealing. In all, 548 structurally important nuclear Overhauser enhancement cross-peaks were quantified as distance restraints and, together with 41 experimentally measured angle restraints, were incorporated into a simulated annealing protocol to determine a family of closely related structures that satisfied the experimental observations. The CCP structure is shown to be based on a beta-sandwich arrangement; one face made up of three beta-strands hydrogen-bonded to form a triple-stranded region at its centre and the other face formed from two separate beta-strands. Both faces of the molecule contribute highly conserved hydrophobic side-chains to a compact core. The regions between the beta-strands are composed of both well-defined turns and less well-defined loops. Analysis of CCP sequence alignments, in light of the determined structure, reveals a high degree of conservation amongst residues of obvious structural importance, while almost all insertions, deletions or replacements observed in the known sequences are found in the less well-defined loop regions. On the basis of these observations it is postulated that models of other CCP modules that are based on the structure presented here will be accurate. Certain families of CCP modules differ from the consensus in that they contain extra cysteine residues. As a test of structural consensus, the extra disulphide bridges are shown to be easily accommodated within the determined CCP model.