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1.
The frequency of Salmonella spp., Shigella spp., Campylobacter spp., Yersinia enterocolitica, Aeromonas hydrophilia and Helminths in samples from various hospitals of the Eastern Cape, South Africa, were analysed for trends involving age distribution and population group. Significant differences in the distribution of these pathogens could be attributed to hygiene and environmental difference between the age and population groups which suggests that rapid urbanization and poor water supply remain major problems in the drought prone Eastern Cape. 相似文献
2.
A M?ynarczyk G M?ynarczyk M ?uczak A Grzesik M Lewandowska J Jeljaszewicz 《Medycyna do?wiadczalna i mikrobiologia》2001,53(3):217-225
S. aureus strains isolated in the same period from different specimens obtained from patients of two different hospitals were compared. The significant differences were observed in the frequency of resistance determinants between strains of these hospitals. The most important was the difference in the prevalence of MRSA. In the first hospital the percentage of MRSA was 40% whereas in the second one only 20%. The resistance to the other antibiotics was also compared, and independently from the compared group: MRSA, MSSA or all, the prevalence of resistance determinants was higher in the first hospital than in the second. Although the frequencies of MRSA in both investigated hospitals were relatively high comparing to the other European countries and in the first hospital even alarming, isolated MRSA strains are less resistant to other antibiotics than MRSA in other countries. 相似文献
3.
《Ostrich》2013,84(1-2):81-86
We compared a data set from the Western Cape, South Africa (GDU, n = 170 Olive Thrushes (Turdus olivaceus), n = 475 Cape Robins (Cossypha caffra)) with our captures in the Eastern Cape, South Africa (n = 197 Olive Thrushes, n = 203 Cape Robins). In both regions Olive Thrushes began moult in December-January, while wing-moult lasted for 89 days in the Western Cape compared to 53 days in the Eastern Cape. Cape Robins began moult in early November in the Western Cape, early January in the Eastern Cape and again the duration of wing-moult was longer in the Western Cape (64 days) than in the Eastern Cape (50 days). For both species the start of moult coincided with the end of the breeding season. Cape Robins were heavier and longer-winged in the Western Cape than in the Eastern Cape. There was no significant difference in mean mass or mean wing length of the Olive Thrush between the two provinces. Both ringing and atlas data suggest that Cape Robins are relatively more common than Olive Thrushes in the Western Cape, but not in the Eastern Cape. In the Eastern Cape we observed colour-ringed robins (n = 2) and thrushes (n = 2) on their breeding territory in all months of the year, suggesting that some individuals of both species are strongly resident. 相似文献
4.
There is general opinion that Staphylococcus aureus strains isolated in hospitals are more frequently resistant to antibiotics than community strains, however, the increasing resemblance between hospital and community strains has been recently reported. The aim of the study was to compare the antibiotic resistance and phage-type pattern of S. aureus strains isolated from patients treated either in hospitals or in general practice in northern part of Poland. The study was conducted on 771 S. aureus strains isolated from different specimens. Phage typing was performed according to the method of Blair and Williams. The drug susceptibility was determined by the disc-diffusion method. There were no significant differences in antibiotic resistance or phage-type pattern when hospital and community methicillin-sensitive S. aureus (MSSA) strains were compared. The most MSSA were resistant to penicillin (84.6% and 82.1% respectively) and doxycycline (49.3% and 50.4% respectively) whereas they were rarely resistant to other antibiotics. The predominance of phage group II was found in both hospitals (28.0%) and general practice (29.9%). Phage group III, usually associated with hospitals, occurred in small percentage (12.9% and 9.4% respectively) while to this group predominantly (76.6%) multiresistant methicillin resistant S. aureus (MRSA) isolated in hospitals belonged. These results suggest, that there is only slight difference in antibiotic resistance between hospital and community S. aureus strains. Antibiotic resistance pattern mainly results from frequency of appearance of MRSA, mostly occurring in hospitals. 相似文献
5.
Borna Müller Violet N. Chihota Manormoney Pillay Marisa Klopper Elizabeth M. Streicher Gerrit Coetzee Andre Trollip Cindy Hayes Marlein E. Bosman Nicolaas C. Gey van Pittius Thomas C. Victor Sebastien Gagneux Paul D. van Helden Robin M. Warren 《PloS one》2013,8(8)
Background
South Africa shows one of the highest global burdens of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB). Since 2002, MDR-TB in South Africa has been treated by a standardized combination therapy, which until 2010 included ofloxacin, kanamycin, ethionamide, ethambutol and pyrazinamide. Since 2010, ethambutol has been replaced by cycloserine or terizidone. The effect of standardized treatment on the acquisition of XDR-TB is not currently known.Methods
We genetically characterized a random sample of 4,667 patient isolates of drug-sensitive, MDR and XDR-TB cases collected from three South African provinces, namely, the Western Cape, Eastern Cape and KwaZulu-Natal. Drug resistance patterns of a subset of isolates were analyzed for the presence of commonly observed resistance mutations.Results
Our analyses revealed a strong association between distinct strain genotypes and the emergence of XDR-TB in three neighbouring provinces of South Africa. Strains predominant in XDR-TB increased in proportion by more than 20-fold from drug-sensitive to XDR-TB and accounted for up to 95% of the XDR-TB cases. A high degree of clustering for drug resistance mutation patterns was detected. For example, the largest cluster of XDR-TB associated strains in the Eastern Cape, affecting more than 40% of all MDR patients in this province, harboured identical mutations concurrently conferring resistance to isoniazid, rifampicin, pyrazinamide, ethambutol, streptomycin, ethionamide, kanamycin, amikacin and capreomycin.Conclusions
XDR-TB associated genotypes in South Africa probably were programmatically selected as a result of the standard treatment regimen being ineffective in preventing their transmission. Our findings call for an immediate adaptation of standard treatment regimens for M/XDR-TB in South Africa. 相似文献6.
During November 1980 to April 1981, 1561 urinary tract pathogens were collected from Turku City Hospital, Turku University Central Hospital, and Kuopio University Central Hospital. Resistance of the strains was tested by agar-plate dilution against trimethoprim, sulphamethoxazole-trimethoprim, sulphamethoxazole, ampicillin, and nitrofurantoin. Resistance to trimethoprim (greater than 8 mg/l) occurred in 8.6-12.2% of strains from the university hospitals (Pseudomonas excluded) and 38.3% of strains from Turku City Hospital. Resistance of Escherichia coli occurred in 4.1-6.2% of strains from the university hospitals and 21% of strains from Turku City Hospital. Proteus mirabilis was the most resistant of the clinically important bacterial species with resistance to trimethoprim in 29-78%. Attention is called for in defining the type of hospital used for a particular study: bacterial resistance in different hospitals cannot be compared direct and one hospital is not necessarily representative for a whole country. After seven years'' use of plain trimethoprim the prevalence of resistance in the two university hospitals in Finland was similar to that in a London hospital just before plain trimethoprim was registered for use in Britain. 相似文献
7.
Levels of aloe mortality with and without elephants in the Thicket Biome of South Africa 总被引:1,自引:1,他引:0
Studies concerning the influence of African elephants ( Loxodonta africana ) on vegetation have produced contradictory results; some show minimal or no effect while others report significant elephant-induced effects. Elephants are generalist megaherbivores but will selectively feed from preferred plant species. We investigated the mortality of aloe plants (highly preferred food items for elephants) at five sites with elephants (treatment) and five paired sites without elephants (control) in the Eastern Cape Province of South Africa. A significantly higher proportion of aloes were dead at treatment sites and significantly more aloes that had lost their crown (headless) were found at treatment sites compared with controls. We conclude that although the proportions of dead aloes at treatment sites were significantly higher, it remains unclear whether there is a need to be concerned with the potential small-scale extinction of aloes from parts of the Eastern Cape Province. The observed mortality may merely be an artefact of the loss of large herbivores through disease (e.g. rinderpest) and hunting in the past. 相似文献
8.
The serological typing of 96% of P. aeruginosa strains isolated in hospitals has been carried out by means of agglutinating sera, and the tendency towards the prevalence of the strains of group V (25.3%) and serovar O11 (18.1%) has been revealed. The determination of the antibiotic sensitivity of different variants has shown that the strains of groups I, V and serovar O11 possess pronounced resistance to a wide spectrum of antibiotics, their resistance to gentamycin and polymixin being higher than in the strains of other serotypes. The determination of antibiotic resistance can serve only as an additional test in the serological typing of P. aeruginosa. 相似文献
9.
Anthony D. Vaudo James D. Ellis Garth A. Cambray Martin Hill 《Journal of Insect Conservation》2012,16(4):601-611
Livestock farming in the Eastern Cape, South Africa is a common land use practice that has affected the biodiversity of plants and animals in the region negatively. Indigenous populations of wild honey bee (Apis mellifera) colonies also may suffer. Recently, farmers in the Eastern Cape have been converting their farms to game reserves as ecotourism attractions and nature conservation lands. Consequently, the goal of this research was to determine if land use habits (livestock farming and conversion to game reserves) in the Eastern Cape affect honey bee colony population density and colony strength parameters. A series of indices were developed to compare the relative population densities of colonies in two or more areas by counting the number of foraging bees and number of bee lines established at feeding stations. Wild colonies on farms and reserves were located and sampled to determine land use effects on colony strength parameters including total area of comb in the colony, the area of comb containing stored honey, pollen, and brood, adult bee population, weight per bee, and the colony nest cavity volume ratio. When viewed collectively, the data indicated that land use practices have affected honey bee nesting dynamics in the Eastern Cape. Trends in the data suggested that colonies nesting on the reserves may occur in greater densities than those nesting on livestock farms, though they do not appear to be healthier. Hopefully, this work will be continued since honey bee conservation in areas where they are native is crucial to the health of agriculture and whole ecosystems globally. 相似文献
10.
Specific Distribution of R Factors in Serratia marcescens Strains Isolated from Hospital Infections 总被引:2,自引:0,他引:2
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Serratia marcescens strains from three hospitals in the city of New York were tested for antibiotic susceptibility patterns and the presence of transmissible antibiotic resistance factors. There appears to be a pattern characteristic for each hospital with regard to the sensitivity to nalidixic acid, tetracycline, chloramphenicol, and sulfonamides, whereas the resistance to ampicillin, cephalothin, and streptomycin is similar in the strains isolated from all three hospitals. In one hospital, a single type of R factor was found which transfers resistance to streptomycin, kanamycin, ampicillin, and sulfonamides, whereas strains isolated from a second hospital transfer only ampicillin resistance. No R factors could be detected in multiply resistant Serratia strains isolated in a third hospital. The presence of a single type of R factor probably reflects the relative ecological isolation of S. marcescens and could be useful for epidemiological studies of hospital infections with Serratia. 相似文献
11.
探讨取食不同寄主植物的2种甜菜夜蛾Spodoptera exigua(Hübner)品系对几种常用药剂的敏感性变化情况。试验结果表明,寄主植物对甜菜夜蛾的生长发育和杀虫剂的敏感性有显著影响,取食不同寄主植物以后,甜菜夜蛾2个品系对溴氰菊酯、毒死蜱、溴虫腈、虫酰肼的敏感性均有不同程度的变化,取食不同寄主植物的甜菜夜蛾蛹黑突变品系较甜菜夜蛾正常品系的抗性均要高,2个品系对溴氰菊酯的敏感性变化均较大,最大LD50之比分别达到13.36、12.30倍,2个品系都对虫酰肼的敏感性变化不明显,最大LD50之比分别达到2.54、2.23倍。 相似文献
12.
Genetic characterization of high-level gentamicin-resistant strains of Enterococcus faecalis in Iran
Feizabadi MM Asadi S Zohari M Gharavi S Etemadi G 《Canadian journal of microbiology》2004,50(10):869-872
The prevalence of resistance to high levels of gentamicin among 182 isolates of Enterococcus faecalis from 2 Iranian hospitals was 42%. Gentamicin resistance was associated with conjugative plasmids (>70 kb) in most strains. Fingerprinting using EcoRI and HindIII showed genetic variation among these plasmids and gave evidence of nosocomial outbreaks and persistence of infection in different wards of the study hospitals, as well as transfer of plasmids between genetically diverse isolates. Using EcoRI, hospital-based specific plasmid fingerprints were detected for the isolates that had previously proved to be unrelated by multilocus enzyme electrophoresis, suggesting the persistence of related plasmids at each hospital, though minor changes in these related plasmids could be detected with HindIII. 相似文献
13.
《African Journal of Aquatic Science》2013,38(2):149-156
The diet and growth of sharptooth catfish, Clarias gariepinus, in an oligotrophic system (Kat River Reservoir, Eastern Cape, South Africa) were compared to those in a eutrophic system (Laing Reservoir, Eastern Cape) to determine if the trophic status of a waterbody had an effect on the growth rate of the species. In order of importance, the diet of catfish in Kat River Reservoir consisted of fish, insects, zooplankton, plant material and other items, while the diet of catfish in Laing Reservoir consisted of fish, plant material, zooplankton, other vertebrates and insects. The diets of catfish in the two reservoirs had a similarity index of 68.1% and there was no significant difference in their nutritional value. Fish prey was the most important dietary component in both reservoirs. Temperature regime and zooplankton and zoobenthos density were similar in both systems. However, fish prey density was significantly higher in the eutrophic Laing Reservoir and catfish grew significantly faster in that system. The slower growth rate in Kat River Reservoir was attributed to the higher energy costs associated with the capture of fish prey, which was less abundant than in Laing Reservoir. Trophic status therefore had an indirect effect on catfish growth by influencing the availability of fish prey. 相似文献
14.
目的:了解张家口地区木糖氧化无色杆菌耐药性的相关基因的基因型别,为临床合理使用抗生素提供实验依据。方法:应用改良三维试验法筛选耐β-内酰胺类药物的菌株,再结合PCR技术和序列测定检测耐药菌ESBLs和AmpC酶的基因型别,最后进行统计学分析。结果:2010年12月~2011年12月本地区临床分离的48株木糖无色杆菌菌株中有32株对β-内酰胺类药物耐药,检出率高达66.7%,其中14株单产ESBLs,5株单产AmpC酶,9株同时产ESBLs和AmpC酶,4株为未知型菌株;筛选出的耐药菌中有24株PCR结果阳性,分属于不同耐药基因型并发现存在多重耐药基因。ESBLs以TEM型检出率最高,均为TEM-1亚型;AmpC酶检出率也较高,均为DHA-1型;多重耐药基因TEM+CTX-M-1+AmpC检出率最高。结论:张家口地区木糖氧化无色杆菌的耐药现象严重,临床上应严格掌握头抱菌素类药物的使用以取得更好的治疗效果。 相似文献
15.
Dmitrenko OA Shaginian IA Gintsburg AL 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2005,(3):11-17
Polymorphism of the chromosome staphylococcus cassette mec (SCCmec), a mobile and heterological genetic element providing resistance to beta-lactam antibiotics was studied in methycillin-resistant strains of Staphylococcus aureus (MRSA) isolated at permanent stations situated in different regions of Russia. Type SCCmec was identified using the PCR method by determining allotypes of 3 different structural genetic complexes incorporated in the cassettes mec. It was found that the isolates studied in this work contained 3 different types of SCCmec: I, III, and IVb. Both isolates containing 2 different copies of SCCmec and isolates containing defective copies of SCCmec were identified. It was demonstrated that determination of the SCC-mec type provided an opportunity to differentiate the isolates studied in this work from one another. The isolates attributed to the same genotype variant (identified by polymorphism of coagulase gene) but isolated at different hospitals located in different regions of Russia were found to contain the same type of the chromosome staphylococcus cassette mec, whereas the isolates of different coagulase groups (i.e., different genotype variants) contained different types of SCCmec. It was found that at least 2 epidemic strains circulated in the permanent hospitals of Russia. The strains differ from one another by the polymorphism of the coagulase gene and the mec DNA polymorphism. According to results of studies of several molecular markers (including mec DNA), these strains proved to be identical to the international strains EMRSA-1 and EMRSA-2. Possible mechanisms of MRSA formation and circulation in Russia and CIS countries are discussed. 相似文献
16.
C. A. Whittington-Jones 《Ostrich》2013,84(2-4):97-103
Whittington-Jones, C.A. 1997. Apparent range expansion of the Redbilled Quelea Quelea quelea in the Eastern Cape Province of South Africa. Ostrich 68 (2–4): 97–103. Distribution records obtained from the South African Bird Atlas Project (SABAP) and from published literature show that the known distribution of Redbilled Quelea in the Eastern Cape has changed dramatically over the last century. Increased search effort following the initiation of SABAP may explain the range expansion, but changes in agricultural practice have probably been a contributing factor. 相似文献
17.
Forty amikacin-resistant strains of Serratia marcescens isolated from four different hospitals (A, B, C, and D) were examined for modifying enzymes and plasmids. Twenty-one of the isolates produced acetyltransferase that modified amikacin. Eighteen of the 21 acetyltransferase-bearing isolates were from different inpatients in hospital A and the other three were from hospital C. Amikacin resistance was mediated by conjugative plasmid of 24 megadaltons in 15 of the 18 acetyltransferase-bearing isolates of hospital A and by nonconjugative plasmids, derivatives of the 24-megadalton plasmids, in the remaining three isolates of the same hospital. The 24-megadalton plasmid determined aminoglycoside acetyltransferase (6') IV. This plasmid-borne enzyme conferred amikacin resistance on S. marcescens but not on Escherichia coli K12. The frequency of transfer of the 24-megadalton plasmid from the S. marcescens isolate to E. coli K12 by conjugation was approximately 10(-7) (transconjugants/donors) and was 0.1% of that between E. coli strains. In acetyltransferase-bearing isolates from hospital C, the enzyme was mediated by a nonconjugative plasmid in one case and could not be associated with a plasmid in the remaining two cases. Neither enzymes nor plasmids could be associated with amikacin resistance of the isolates of the other two hospitals. 相似文献
18.
We evaluated the incidence of Haemophilus influenzae resistance to selected antimicrobials used in Canada. From 1985 to 1987, 2503 H. influenzae isolates obtained in 14 hospitals across Canada were sent to the Centre hospitalier de l''université Laval (CHUL) for identification, serotyping, biotyping and testing for beta-lactamase production. Susceptibility tests were done with the use of 12 antibiotics. Of the strains 424 (16.9%) produced beta-lactamase; the proportion varied from 12.8%, in Newfoundland, to 19.6%, in Ontario. Of the strains 18.3% were type b; 19.4% of those produced beta-lactamase. Almost 82% of the strains were not type b. The proportion of beta-lactamase-producing strains varied according to the isolation site, from 15.3% in the respiratory tract to 25.6% in the blood. The overall level of resistance was 19.3% to ampicillin, 24.2% to erythromycin, 3.8% to trimethoprim-sulfamethoxazole, 1.7% to amoxicillin-potassium clavulanate, 1.4% to cefaclor, 1.3% to tetracycline, 1.0% to rifampin, 0.7% to cefuroxime and 0.1% to cefamandole. Disc diffusion susceptibility testing revealed 64 strains (2.6%) that did not produce beta-lactamase but were resistant to ampicillin and 9 (0.4%) that produced beta-lactamase but were susceptible to ampicillin. The results of beta-lactamase production tests were identical regardless of whether the tests were done by the CHUL or by the other hospitals, but there was a marked difference in the susceptibility test results between the CHUL and the other centres. Our results suggest that the level of resistance of H. influenzae to antibiotics is increasing in Canada and that the initial choice of drug therapy may have to be modified. 相似文献
19.
Lázaro Molina Zulema Udaondo Estrella Duque Matilde Fernández Carlos Molina-Santiago Amalia Roca Mario Porcel Jesús de la Torre Ana Segura Patrick Plesiat Katy Jeannot Juan-Luis Ramos 《PloS one》2014,9(1)
Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from in-patients in different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority of the antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics, macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. To shed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genome of this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are both chromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibiotic and biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens. The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts. 相似文献
20.
Alexey V. Altukhov Russel D. Andrews Donald G. Calkins Thomas S. Gelatt Eliezer D. Gurarie Thomas R. Loughlin Evgeny G. Mamaev Victor S. Nikulin Peter A. Permyakov Sergey D. Ryazanov Vladimir V. Vertyankin Vladimir N. Burkanov 《PloS one》2015,10(5)
After a dramatic population decline, Steller sea lions have begun to recover throughout most of their range. However, Steller sea lions in the Western Aleutians and Commander Islands are continuing to decline. Comparing survival rates between regions with different population trends may provide insights into the factors driving the dynamics, but published data on vital rates have been extremely scarce, especially in regions where the populations are still declining. Fortunately, an unprecedented dataset of marked Steller sea lions at rookeries in the Russian Far East is available, allowing us to determine age and sex specific survival in sea lions up to 22 years old. We focused on survival rates in three areas in the Russian range with differing population trends: the Commander Islands (Medny Island rookery), Eastern Kamchatka (Kozlov Cape rookery) and the Kuril Islands (four rookeries). Survival rates differed between these three regions, though not necessarily as predicted by population trends. Pup survival was higher where the populations were declining (Medny Island) or not recovering (Kozlov Cape) than in all Kuril Island rookeries. The lowest adult (> 3 years old) female survival was found on Medny Island and this may be responsible for the continued population decline there. However, the highest adult survival was found at Kozlov Cape, not in the Kuril Islands where the population is increasing, so we suggest that differences in birth rates might be an important driver of these divergent population trends. High pup survival on the Commander Islands and Kamchatka Coast may be a consequence of less frequent (e.g. biennial) reproduction there, which may permit females that skip birth years to invest more in their offspring, leading to higher pup survival, but this hypothesis awaits measurement of birth rates in these areas. 相似文献