共查询到20条相似文献,搜索用时 15 毫秒
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Dennis Francis 《Phytochemistry》2002,59(2):233-234
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Toni Slabas 《Phytochemistry》2002,59(2):229
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Stephen C Fry 《Phytochemistry》2002,59(2):230-231
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Barry Halliwell 《Phytochemistry》2002,59(2):232-233
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Michael W. Fowler 《Biotechnology & genetic engineering reviews》2013,29(1):41-67
Abstract Reporter gene assay is an invaluable tool for both biomedical and pharmaceutical researches to monitor cellular events associated with gene expression, regulation and signal transduction. On the basis of the alternations in reporter gene activities mediated by attaching response elements to these reporter genes, one sensitive, reliable and convenient assay can be provided to efficiently report the activation of particular messenger cascades and their effects on gene expression and regulations inside cells or living subjects. In this review, we introduce the current status of several commonly used reporter genes such as chloramphenicol acetyltransferase (CAT), alkaline phos-phatase (AP), β-galactosidase (β-gal), luciferases, green fuorescent protein (GFP), and β-lactamase. Their applications in monitoring gene expression and regulations in vitro and in vivo will be summarized. With the development of advanced technology in gene expression and optical imaging modalities, reporter genes will become increasingly important in real-time detection of the gene expression at the single-cell level. This synergy will make it possible to understand the molecular basis of diseases, track the effectiveness of pharmaceuticals, monitor the response to therapies and evaluate the development process of new drugs. 相似文献
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