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1.
G. B. Khomutov 《Biophysics》2011,56(5):843-857
Biophysicochemical approaches to the solution of nanotechnology problems associated with the design of functional biomimetic
nanosystems, hybrid and composite nanobiomaterials and study of their structure-function relationships. The results of studies
concerned with physicochemical mechanisms of the formation of organized biomimetic nanostructures and bioinorganic nanomaterials
in systems involving a bulky liquid phase and the interface (gas-liquid, solid-liquid, liquid-liquid)during the synthesis
and structure formation with the participation of the components of colloid systems, inorganic nanoparticles of various composition
and clusters of metals, surfactants, polyelectrolytes and their complexes are discussed. In the development of the methods
for the formation of composite bioinorganic nanosystems containing inorganic nanocomponents, two major approaches were used:
adsorption and incorporation into the biomolecular matrix or colloid system of presynthesized inorganic nanoparticles, as
well as the synthesis of the inorganic nanophase immediately in the biomolecular system. The methods of obtaining biomaterials
and nanosystems are based on the principles of biomimetics, biomineralization, self-assembly and self-organization, combination
and integration of a number of synthetic and physicochemical methods (physical and chemical adsorption, Langmuir technique,
the formation of polycomplexes, chemical linking, competitive interactions, and substitution of ligands in supramolecular
and coordination complexes) and nanocomponents of different nature. In particular, a novel approach to the preparation of
highly organized nanofilm materials was developed, which is based on the effect of self-assembly and self-organization of
colloid nanoparticles during the formation of their complexes with polyfunctional biogenic ligands in the volume of the liquid
phase in the absence of any surfaces and interfaces. The physical and chemical factors responsible for the formation of structurally
ordered biomolecular and composite nanosystems including nano-sized components of different nature and the possibilities to
control the composition, structure, and properties of resulting nanomaterials and nanosystems are discussed. The experimental
methods and approaches developed may be useful in studies of structure-property relationships and basic mechanisms of structural
organization and transformation at the nanoscales level in biological, artificial, and hybrid nanosystems. The problems of
practical application of the synthetic methods and the corresponding nanomaterials are discussed. 相似文献
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Siddheshwar K. Chauthe Sandip B. Bharate Sudeep Sabde Debashis Mitra Kamlesh K. Bhutani Inder P. Singh 《Bioorganic & medicinal chemistry》2010,18(5):2029-2036
Plants are an important source of a variety of bioactive compounds with different modes of action. Anti-HIV agents from plant sources can be useful in developing novel therapies for inhibiting HIV infection. Based on the reported anti-HIV activity of plant derived phloroglucinols, several new dimeric phloroglucinols were synthesized in the present study by varying substitution on aromatic ring and at methylene bridge. Some of the synthesized compounds have shown good HIV inhibitory activity in a human CD4+ T cell line (CEM-GFP) infected with HIV-1 NL4.3 virus isolate. Structure–activity studies indicate that phenyl, 4-benzyloxy-1-phenyl and cyclohexyl substitution at methylene bridge gave compounds with better anti-HIV activity. Compounds 22 and 24 showed highest anti-HIV activity with an IC50 of 0.28 μM and 2.71 μM, respectively, former was more active than the positive standard AZT in cell based assay. 相似文献
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Zhang YZ Su B Venugopal J Ramakrishna S Lim CT 《International journal of nanomedicine》2007,2(4):623-638
Electrospinning is an enabling technology that can architecturally (in terms of geometry, morphology or topography) and biochemically fabricate engineered cellular scaffolds that mimic the native extracellular matrix (ECM). This is especially important and forms one of the essential paradigms in the area of tissue engineering. While biomimesis of the physical dimensions of native ECM's major constituents (eg, collagen) is no longer a fabrication-related challenge in tissue engineering research, conveying bioactivity to electrospun nanofibrous structures will determine the efficiency of utilizing electrospun nanofibers for regenerating biologically functional tissues. This can certainly be achieved through developing composite nanofibers. This article gives a brief overview on the current development and application status of employing electrospun composite nanofibers for constructing biomimetic and bioactive tissue scaffolds. Considering that composites consist of at least two material components and phases, this review details three different configurations of nanofibrous composite structures by using hybridizing basic binary material systems as example. These are components blended composite nanofiber, core-shell structured composite nanofiber, and nanofibrous mingled structure. 相似文献
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Roy S Fortier JM Nagarajan R Tripathy S Kumar J Samuelson LA Bruno FF 《Biomacromolecules》2002,3(5):937-941
A new biomimetic route for the synthesis of a conducting molecular complex of polyaniline (Pani) and a natural polyelectrolyte, lignosulfonate (LGS) is presented. A poly(ethylene glycol) modified hematin (PEG-hematin) was used to catalyze the polymerization of aniline in the presence of LGS to form a Pani/LGS complex. UV-vis, FTIR, conductivity and TGA studies for the LGS-polyaniline complex indicate the presence of a thermally stable and electrically conductive form of polyaniline. Also the presence of LGS in this complex, an inexpensive byproduct from pulp processing, provides a unique combination of properties such as electronic conductivity, processability and biodegradability. The use of this conductive complex for corrosion protection is also proposed. 相似文献
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A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by fungal proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72h which could be reduced to 1h by tailoring the reaction conditions. Under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. The resulting Ag NPs displayed controllable structural and optical properties depending on the experimental parameters such as pH and reaction temperatures. The average size, morphology, and structure of particles were determined by AFM, TEM, XRD and UV/Visible absorption spectrophotometry. Fourier transform infrared study disclosed that the amino groups were bound to the particles, which was accountable for the stability of NPs. It further confirmed the presence of protein as the stabilizing and capping agent surrounding the silver NPs. Experiments were conducted both with, media in which fungus was initially harvested and that of pristine fungal mycelium alone. Under normal conditions, in the case of media extracellular synthesis took place whereby other than the fungal proteins, glucose was also responsible for the reduction. In the case of fungal mycelium, the intracellular formation of Ag NPs, could be tailored to give both intracellular and extracellular Ag NPs under alkaline conditions whereby the surface S-H groups of the fungus played a major role. 相似文献
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Volgraf M Lumb JP Brastianos HC Carr G Chung MK Münzel M Mauk AG Andersen RJ Trauner D 《Nature chemical biology》2008,4(9):535-537
Biomimetic synthesis is an attempt to assemble natural products along biosynthetic lines without recourse to the full enzymatic machinery of nature. We exemplify this with a total synthesis of exiguamine A and the newly isolated natural product exiguamine B. The most noteworthy feature of this work is an oxidative endgame drawing from the complex chemistry of catecholamines, which allows for ready access to a new class of nanomolar indoleamine-2,3-dioxygenase inhibitors. 相似文献
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Pekkurnaz G Fera A Zimmerberg-Helms J Degiorgis JA Bezrukov L Blank PS Mazar J Reese TS Zimmerberg J 《The Biological bulletin》2011,220(2):89-96
Synaptic vesicles contain a variety of proteins and lipids that mediate fusion with the pre-synaptic membrane. Although the structures of many synaptic vesicle proteins are known, an overall picture of how they are organized at the vesicle surface is lacking. In this paper, we describe a better method for the isolation of squid synaptic vesicles and characterize the results. For highly pure and intact synaptic vesicles from squid optic lobe, glycerol density gradient centrifugation was the key step. Different electron microscopic methods show that vesicle membrane surfaces are largely covered with structures corresponding to surface proteins. Each vesicle contains several stalked globular structures that extend from the vesicle surface and are consistent with the V-ATPase. BLAST search of a library of squid expressed sequence tags identifies 10 V-ATPase subunits, which are expressed in the squid stellate ganglia. Negative-stain tomography demonstrates directly that vesicles flatten during the drying step of negative staining, and furthermore shows details of individual vesicles and other proteins at the vesicle surface. 相似文献
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Donohoe BS Vinzant TB Elander RT Pallapolu VR Lee YY Garlock RJ Balan V Dale BE Kim Y Mosier NS Ladisch MR Falls M Holtzapple MT Sierra-Ramirez R Shi J Ebrik MA Redmond T Yang B Wyman CE Hames B Thomas S Warner RE 《Bioresource technology》2011,102(24):11097-11104
The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields. 相似文献
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BackgroundThe threat of cadmium (Cd), which is the cause of itai-itai disease in Japan, is still complicated and confusing, especially for digestive system, such as liver disease. One of the most keys of this problem is demonstrating that the hepatotoxicity is indeed induced by Cd. Therefore, we attempt detecting Cd at microscale during ultrastructural imaging of liver tissue.Methods12 rats were divided randomly into two experimental groups: control and Cd-treated. Treated rats were intraperitoneal injected with 1 mg/kg body weight cadmium chloride (CdCl2) for 4 weeks (5 P.M each day for 6 days/week). At the end of the exposure period, liver tissue samples were processed into ultrathin sections for analysis of advanced analytical transmission electron microscopy and X-ray energy dispersive spectroscopy (TEM/X-EDS) investigations. Ultrastructural images and X-ray energy dispersive spectrum were acquired at microscale.ResultsCd can cause changes in the structure of the organelle, including the collapse of the membrane structure in the cell, the destruction of the internal structure of the organelle, the mitochondrial swelling, the expansion of the endoplasmic reticulum, and the appearance of inclusions. Cadmium bioaccumulation is detected in the mitochondria at microscale by TEM/X-EDS, which is the visual evidence of morphological changes of mitochondria related to Cd.ConclusionThe combination of detailed ultrastructure and microscale X-ray energy dispersive spectroscopy (X-EDS) characterization of cadmium hepatotoxicity demonstrate that cadmium indeed leads to mitochondrial damage, which is helpful for further investigation of the pathological mechanism of cadmium hepatotoxicity. 相似文献
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The free circulating coelomocytes in the coelomic cavity of echinoderms are considered to be immune effectors by phagocytosis, encapsulation, cytotoxicity, and by the production of antimicrobial agents. Although echinoderms (especially sea urchin embryo) have been used as a model organisms in biology, no uniform criteria exist for classification of coelomocytes in echinoderms, and few studies have reported about the biological functions of their coelomocytes. Hence, we study the coelomocytes in the echinoid sea urchin, Paracentrotus lividus, and describe their morphological and ultrastructural features using light and transmission electron microscopes. We classify the coelomocytes of P. lividus into red spherule and colorless spherule cells, small cells, vibratile cells, and phagocytic cells; petaloid and filopodial cells. To our knowledge, this is the first report describing ultrastructural details of the coelomocytes of P. lividus. J. Morphol. 276:583–588, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
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The aim of the present study was to characterize the ultrastructure of zebu cow preantral follicles (PAFs). Ovarian cortex samples were processed for light and transmission electron microscopy. Primordial follicles consisted of an oocyte surrounded by one layer of flattened or flattened-cuboidal granulosa cells. The oocyte contained a large and usually eccentric nucleus. Most organelles were located at the perinuclear ooplasm. Round shaped mitochondria, which contained electron-dense granules, smooth and rough endoplasma reticulum and a Golgi apparatus were also observed. Vesicles and coated pits were often observed in the cortical ooplasm. In primary follicles, the oocyte was surrounded by one layer of cuboidal granulosa cells. Short microvilli were observed on the oolema. Secondary follicles consisted of an oocyte surrounded by a variable number of layers of cuboidal granulosa cells. Small secondary follicles had an ultrastructure very similar to that observed in primary follicles. At this follicular stage, the zona pellucida was beginning to form around the oocyte. In large secondary follicles, the zona pellucida was totally developed around the oocyte. Several granulosa cell projections could be detected that were encroaching into the zona pellucida and protruding towards the oocyte, where gap junctions were observed between oocyte and granulosa cell membranes. Organelles within the oocyte were located at the periphery of the ooplasm, and clusters of cortical granules were observed. Round mitochondria were abundant in all developmental stages. In conclusion, this study described the ultrastructure of zebu cow PAFs, and some unique characteristics could be observed as compared with what has been reported for follicles of Bos taurus cattle. 相似文献
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Genetic analysis of a strain of Drosophila melanogaster revealed that a recessive mutation [l(1)ERts] causing temperature-sensitive embryonic lethality is located in the distal region of the X chromosome approximately at map position 18. At 22–25°C mutant embryos exhibit normal viability, and all eggs arrest prior to gastrulation if they are reared at 29°C. The mutant is biphasic, exhibiting a maternal effect which is expressed throughout the first 8 hr of development as well as a second temperature-sensitive period (TSP) during the first 3 days of larval life. Larvae exposed to the restrictive temperature (RT) during the second TSP must also spend the remainder of larval and pupal life and the time of normal eclosion at RT to die as fully developed pupae which fail to eclose. Light and electron microscopy of arrested embryos reveal disturbances in the distribution of nuclei, cytoplasm, and yolk and abnormal configurations of rough endoplasmic reticulum. The cause of pupal death during the second lethal period is unknown. 相似文献
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Isolation and ultrastructural characterization of the residual nuclear matrix in a plant cell system
We isolated the nuclear matrix of proliferative meristematic root cells of Allium cepa bulbs and characterized it ultrastructurally and by one-dimensional SDS-polyacrylamide gel electrophoresis. The general organization of the nuclear matrix in onion root cells is similar to that found in other eukaryotes extracted under the same conditions. There are three main morphological components: the residual pore-lamina complex, the internal matrix and the residual nucleolus. The matrix has a fibrillo-granular structure composed of 5–10 nm fibrils and 20–25 nm granules. The residual protein matrix has a complex pattern, with several polypeptide bands of 12000 to 70000 Mr. The major bands have Mrs of 62000, 56000, 35000 and 27000.by M. Trendelenburg 相似文献
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Immunocytochemical and ultrastructural characterization of endocrine cells in chicken proventriculus
Summary The endocrine cells of the chicken proventriculus were investigated immunocytochemically, using the peroxidase-antiperoxidase technique on paraffin and semithin sections for light microscopy, and immunogold staining in osmium-fixed material for electron microscopy. The fixation procedure also allowed a detailed ultrastructural investigation. Twenty-three antisera were tested and 7 immunoreactive cell-types were identified: D-cells containing somatostatin-like peptide; EG-cells immunoreactive to anti-glucagon, anti-GLP1 and antineurotensin; NT-cells labelled only with anti-neurotensin; BN-cells containing bombesin-like material; ENK-cells showing met-enkephalin immunoreactivity; EC-cells reactive to anti-serotonin; and APP-cells positive to anti-avian pancreatic polypeptide. In addition, enterochromaffin-like (ECL) cells, were also detected by electron microscopy. The presence of ENK-cells and the ultrastructure of these and NT-cells are described for the first time in chicken proventriculus, and glucagon, GLP1 and neurotensin are shown to be colocalized in the EG-cells. 相似文献
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Jacobsen NE Wijeratne EM Corsino J Furlan M Bolzani Vda S Gunatilaka AA 《Bioorganic & medicinal chemistry》2008,16(4):1884-1889
Reaction of pristimerin with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) resulted in a biomimetic-type coupling leading to xuxuarines Ealpha and Ebeta and not the previously reported Rzedowskia bistriterpenoids I and II suggesting that the structures proposed for these natural products need revision. A product obtained in this reaction by an unusual Diels-Alder addition followed by retro-Diels-Alder-type elimination was characterized as pristimerin dicyanophenalenedione. Complete 1H, and 13C NMR spectral assignments of xuxuarines Ealpha and Ebeta have been made by the application of 1D and 2D NMR techniques. 相似文献