酵母杂交系统的发展及其应用

近年来,酵母单杂交,双杂交及三杂交系统技术的出现及发展已成为分析鉴定蛋白－蛋白,DNA－蛋白相互作用及调控的强有力工具,本文简述了酵母杂交系统的发展及潜在的应用价值。     

酵母正反向n-杂交系统研究进展

介绍酵母单杂交、双杂交、三杂交等一系列酵母正向n 杂交系统的原理、研究进展以及应用 ,同时对在此基础上发展出的酵母反向n 杂交系统的原理、研究进展、应用前景等也进行了综述。     

酵母正反向n-杂交系统研究进展

介绍酵母单杂交、双杂交、三杂交等一系列酵母正向n-杂交系统的原理、研究进展以及应用,同时对在此基础上发展出的酵母反向n-杂交系统的原理、研究进展、应用前景等也进行了综述。     

酵母三杂交系统的原理和应用

酵母双杂交系统自出现以来,广泛用于研究蛋白质之间的相互作用,它是一种具有高灵敏度的研究蛋白质之间关系的技术.在酵母双杂交系统基础上发展的酵母三杂交系统将应用范围扩展到蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子化合物等更广阔的研究领域.本文着重介绍酵母三杂交系统的原理、应用及局限性.     

酵母三杂交系统的应用研究进展

1996年,酵母三杂交技术被研究应用于筛选RNA结合蛋白.本介绍了酵母三杂交系统的原理、应用、前景和存在的不足及局限,并分析其原因,总结了在过去8年中利用此方法所取得的成就.在酵母双杂交基础上发展起来的酵母三杂交系统,其应用范围已扩大到蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子药物间的相互作用等更加广泛的研究领域.     

酵母三杂交系统的应用研究进展

介绍了酵母三杂交系统的原理、应用、前景和存在的不足.在酵母双杂交基础上发展起来的酵母三杂交系统,将应用范围扩大到研究蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子药物间的相互作用.     

酵母杂交系统的改进与研究进展

蛋白-蛋白相互作用在细胞生命活动中起着关键的作用。后基因组时代主要是研究蛋白的功能,通过研究蛋白的相互作用及其图谱的构建来了解蛋白的功能。研究蛋白-蛋白相互作用的方法很多,酵母杂交是最常用的检测体内蛋白-蛋白相互作用的敏感系统。随着人们对该系统的广泛应用。这一系统得到了不断的完善及改进,并广泛应用于蛋白-蛋白、蛋白质-RNA、蛋白质-DNA以及其他的小分子间相互作用的研究。拟就酵母杂交系统的原理、改进、发展及应用做一综述。     

酵母双杂交系统及其应用

酵母双杂交系统（yeast two-hybrid system)是直接于细胞内研究蛋白质间相互作用的一种灵敏度很高,且非常有效的遗传学方法,在不同研究领域中广泛应用,并不断完善及改进,发展出单杂交系统（one-hybid system)和三杂交系统（three-hybrid system)等一系列相关的技术,克服了原系统的局限,本文对双杂交系统的原理,发展概况,应用,存在问题和前景等进行了综述。     

基于转录重建的生物大分子相互作用的研究方法

生物大分子的相互作用对生命有极其重要的作用,本文主要介绍了基于转录重建的生物大分子在相互作用的研究方法,包括酵母双杂交系统,单杂交系统和三杂交系统等技术的共同背景,各方法的建立,原理及主要用途。     

酵母双杂交系统研究及其应用进展

综述了自酵母双杂交系统建立以来主要的研究及其应用进展状况,包括:基本原理、一般操作程序、杂交系统研究发展历程、主要应用研究进展、存在问题及其前景等。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.