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1.
Arribére MA Ribeiro Guevara S Bubach DF Arcagni M Vigliano PH 《Biological trace element research》2008,122(1):42-63
A survey of mercury (Hg) and selenium (Se) contents was performed in fish collected from lakes located in two National Parks
of the northern patagonian Andean range. Two native species, catfish (Diplomystes viedmensis) and creole perch (Percichthys trucha), and three introduced species, brown trout (Salmo trutta), rainbow trout (Oncorhynchus mykiss), and brook trout (Salvelinus fontinalis), were caught from lakes Nahuel Huapi, Moreno, Traful, Espejo Chico, and Guillelmo belonging to Nahuel Huapi National Park
and from lakes Futalaufquen and Rivadavia, Los Alerces National Park. In lake Moreno, fish diet items were analyzed and rainbow
trout grown in a farm. Hg and Se were measured in muscle and liver tissues by instrumental neutron activation analysis. The
average concentrations in muscle of Hg for all species, ages, and lakes are between 0.4 to 1.0 μg g−1 dry weight (DW) with a few fish, mainly native, exceeding the United States Environmental Protection Agency health advisory
for freshwater fish limited consumption, and from 0.8 to 1.5 μg g−1 DW for Se. Average concentrations in liver of Hg in all species range from 0.4 to 0.9 μg g−1 DW. Brown trout, the top predator in these lakes, showed the lowest average Hg burden in both tissues. Se concentrations
in the liver of brown and rainbow trout, up to 279 μg g−1 DW, are higher than those expected for nearly pristine lakes, exceeding 20 μg g−1 DW, the threshold concentration associated with Se toxicity. These species show lower Hg contents in muscle, suggesting a
possible detoxification of Hg by a Se-rich diet. Creole perch and velvet catfish livers have lower Se concentrations, with
a narrower span of values (2.3 to 8.5 μg g−1 and 3.3 to 5.5 μg g−1 DW respectively). 相似文献
2.
Fort DJ Propst TL Stover EL Strong PL Murray FJ 《Biological trace element research》1998,66(1-3):237-259
Frog embryo teratogenesis assay—Xenopus (FETAX) was utilized as a model system to evaluate the effects on embryo-larval development at various low boron (B) exposure
levels in the culture media. Concentrations tested ranged from <1 to 5000 μg B/L. A statistically significant (P < 0.05) increase
in malformations was observed at ≤ 3 μg B/L, but not at the greater concentrations. Abnormal development of the gut, craniofacial
region and eye, visceral edema, and kinking of the tail musculature (abnormal myotome development) and notochord were observed.
In subsequent studies, adult frogs were maintained for 28 d on two diets: (1) low B (LB, 62 μg B/kg) or (2) boric acid supplemented
(BA, 1851 μg B/kg); the frogs were subsequently mated, and their offspring were cultured in media containing various levels
of B. Results of the 28-d depletion studies indicated that frogs maintained under LB conditions produced a greater proportion
of (1) necrotic eggs and (2) fertilized embryos, which abnormally gastrulated at a greater rate and were substantially less
viable than embryos from frogs fed the BA diet. Malformations similar to those seen in the initial study were observed in
embryos from the B-depleted adults maintained in an LB environment; 28 d on the LB diet enhanced the incidence of malformations
associated with the LB culture media. These abnormalities were not observed in embryos cultured in ≥4 μg B/L from adults cultured
on the BA diet. These studies showed that insufficient B reproducibly interfered with normalXenopus laevis development during organogenesis, substantially impaired normal reproductive function in adult frogs, and thus represent
the first studies demonstrating the nutritional essentiality of B in an amphibian species. 相似文献
3.
Meyer JS Boelter AM Woodward DF Goldstein JN Farag AM Hubert WA 《Biological trace element research》1998,66(1-3):167-184
The Firehole River (FHR) in Yellowstone National Park (YNP) is a world-renowned recreational fishery that predominantly includes
rainbow trout (RBT,Oncorhynchus mykiss) and brown trout (BNT,Salmo trutta). The trout populations apparently are closed to immigration and have been self-sustaining since 1955. Inputs from hot springs
and geysers increase the temperature and mineral content of the water, including elevating the boron (B) concentrations to
a maximum of 1 mg B/L. Both RBT and BNT reside in warm-water reaches, except when the water is extremely warm (≥25°C) during
midsummer. They spawn in late fall and early winter, with documented spawning of BNT in the cold-water reach upstream from
the Upper Geyser Basin and of RBT in the Lower Geyser Basin reach, where water temperatures presumably are the warmest; however,
successful recruitment of RBT in waters containing 1 mg B/L has not been demonstrated conclusively. Thus, we began investigating
the relationships among temperature, B concentrations, other waterquality parameters, and the distribution and reproduction
of trout in the FHR in spring 1997. However, atypical high water flows and concomitant lower than historical temperatures
and B concentrations during summer 1997 preclude conclusions about avoidance of high B concentrations. 相似文献
4.
Trion A Schutte-Bart C Bax WH Jukema JW van der Laarse A 《Molecular and cellular biochemistry》2008,308(1-2):25-33
Background Vascular calcification is an organized process in which vascular smooth muscle cells (VSMCs) are implicated primarily. The
purpose of the present study was to assess the effects of calcium antagonists and statins on VSMC calcification in vitro.
Methods VSMC calcification was stimulated by incubation in growth medium supplemented with 10 mmol/l β-glycerophosphate, 8 mmol/l
CaCl2, 10 mmol/l sodium pyruvate, 1 μmol/l insulin, 50 μg/ml ascorbic acid, and 100 nmol/l dexamethasone (calcification medium).
Calcification, proliferation, and apoptosis of VSMCs were quantified. Results Calcium deposition was stimulated dose-dependently by β-glycerophosphate, CaCl2, and ascorbic acid (all P < 0.01). Addition of amlodipine (0.01–1 μmol/l) to the calcification medium did not affect VSMC calcification. However, atorvastatin
(2–50 μmol/l) stimulated calcium deposition dose-dependently. Combining treatments stimulated calcification to a degree similar
to that observed with atorvastatin alone. Both atorvastatin and amlodipine inhibited VSMC proliferation at the highest concentration
used. Only atorvastatin (50 μmol/l) induced considerable apoptosis of VSMCs. Conclusion In vitro calcification of VSMCs is not affected by amlodipine, but is stimulated by atorvastatin at concentrations ≥10 μmol/l,
which could contribute to the plaque-stabilizing effect reported for statins.
J. W. Jukema is an Established Clinical Investigator of The Netherlands Heart Foundation 2001D032. 相似文献
5.
Jerome Honnorat Michele Accominotti Christiane Broussolle Andree-Carole Fleuret Jean-Jacques Vallon Jacques Orgiazzi 《Biological trace element research》1992,32(1-3):311-316
Zinc status was assessed in 53 diabetic patients: 18 insulin-dependent diabetic patients (IDDM), 22 noninsulin-dependent diabetic
patients (NIDDM) treated with oral antidiabetic agents, and 13 insulin-treated, noninsulin-dependent diabetic patients (IRDM).
Plasma zinc concentrations were in the usual range for healthy subjects in these three groups (15.3±0.9 μmol/L). Urinary zinc
excretions were elevated in the IDDM group (18.3±4.1 μmol/24 h;p<0.01 vs normal) and in the NIDDM group (17.5±3.5 μmol/24 h;p<0.01 vs normal), but normal in the IRDM group (11.3±2.4 μmol/24 h). In 14 NIDDM patients treated with transient continuous
sc insulin injections, urinary zinc decreased from 16.5±2.2 μmol/24 h before insulin treatment to 11.5±0.3 μmol/24 h after
insulin treatment without any modification in plasma zinc concentrations. 相似文献
6.
J. Grones 《Folia microbiologica》1991,36(3):229-233
At a concentration of 0.5 to 3 mmol/L, ATP stimulates the activity of mitochondrial DNA polymerase ofNeurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate
(1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmaleimide (2 mmol/L) has no effect. Ethidium
bromide (up to 10 μmol/L) and heparin (up to 0.4 μg/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase
reaction. The bestin vitro template is the activated calf-thymus DNA.
2nd report of the series Mitochondrial DNA polymerase from the poky mutant ofNeurospora crassa; 1st report:Biológia (Bratislava)
45, 601–606 (1990).
Translated by Č. Novotny 相似文献
7.
Bollo E Guglielmino R Sant S Pregel P Riondato F Miniscalco B Cornaglia E Nebbia C Dacasto M 《Cell biology and toxicology》2006,22(4):275-284
Triphenyltin acetate (TPTA), a triorganotin compound used in agriculture as a biocide, is immunotoxic in vivo and in vitro. The present study was undertaken to ascertain whether apoptosis might play a role in the TPTA toxicity in vitro. Mouse thymocyte primary cultures were exposed to 0, 4 and 8 μmol/L TPTA; methyl prednisolone (1 μmol/L) was used as a positive
control. Cell aliquots were harvested after 0, 1, 2, 4, and 8 h and the presence of early or late apoptotic phenomena was
checked by (a) morphological investigations; (b) spectrophotometric quantification of fragmented DNA and agarose gel electrophoresis;
(c) cell flow cytofluorometry, using an annexin V-FITC kit; and (d) detection of in situ apoptosis by a colorimetric detection kit (Titer-Tacs). TPTA cytotoxicity was also evaluated using the trypan blue dye exclusion
test. Morphological investigation indicated apoptosis and/or necrosis. After 8 h of incubation, cells exposed to 4 μmol/L
TPTA showed an increase in DNA fragmentation (on electrophoresis), which was confirmed by spectrophotometry (p < 0.05). Flow cytofluorometry pointed out an early (p < 0.05) increase of annexin V-positive (apoptotic) cells in TPTA-exposed flasks, whereas at least partly contradictory, results
were obtained with the Titer-Tacs kit. Overall, these results provide evidence that TPTA, at low concentrations (4 μmol/L)
induces early and late apoptotic phenomena, whereas cells exposed to the highest concentrations (8 μmol/L) are likely to undergo
necrosis rather than apoptosis. 相似文献
8.
Zhi-Hua Li Vladimir Zlabek Roman Grabic Ping Li Tomas Randak 《Central European Journal of Biology》2011,6(1):84-90
The aim of this study is to investigate the toxic effect of PCZ, a triazole fungicide commonly present in surface and ground
water, on the ROS defense system and Na+-K+-ATPase in gills of rainbow trout exposed to sublethal concentrations (0.2, 50 and 500 μg L−1) for 7, 20 and 30 days. After prolonged exposure of PCZ at higher test concentrations (50 and 500 μg L−1), oxidative stress was apparent as reflected by the significant higher ROS levels in fish gill, as well as the significant
inhibition of SOD and CAT activities. In addition, Na+-K+-ATPase activities were significantly lower than those of the control with increasing PCZ concentration and prolonged exposure
period. The results of this study indicate that chronic exposure to PCZ has altered multiple physiological indices in fish
gill; however, before these parameters are used as unique biomarkers for monitoring residual pharmaceuticals in aquatic environments,
more detailed laboratory experiments need to be performed. 相似文献
9.
Abdelmouttaleb I Danchin N Aimone-Gastin I Namour F Angioi M Gelot MA Bennani N Lambert D Jeandel C Guéant JL 《Amino acids》2000,18(2):139-146
Summary. Homocysteine and vitamins B were correlated with coronary artery disease in patients undergoing diagnostic coronary angiography.
160 patients having ≧1 stenosis (G1), 55 patients having normal coronary arteries (G2) and 171 healthy volunteers (G3) were
prospectively recruited.
Homocysteine levels were significantly higher in patients, particulary in those with normal coronary angiograms, than in healthy
subjects (13.8 ± 6.3 μmol/L in G1 (p < 0.0001) and 15.2 ± 8.8 μmol/L in G2 (p < 0.0001) versus 10.1 ± 3.1 μmol/L in G3). Homocysteine levels were not related to the extent of coronary artery disease. In patients with normal angiogram,
vitamin B12 and folate levels were significantly higher compared with the other groups (p < 0.05 and p < 0.001, respectively)
showing that vitamin B deficiency was not involved in the hyperhomocysteinemia.
In conclusion, homocysteine and vitamins B levels do not contribute to discriminate for the presence of coronary artery disease
in patients undergoing diagnostic coronary angiography. Homocysteine levels, however, were higher in patients referred for
coronary angiography than in healthy controls.
Received November 7, 1998, Accepted February 20, 1999 相似文献
10.
To gain insight into the metabolic design of the amino acid carrier systems in fish, we injected a bolus of 15N amino acids into the dorsal aorta in mature rainbow trout (Oncorhynchus mykiss). The plasma kinetic parameters including concentration, pool size, rate of disappearance (R
d), half-life and turnover rate were determined for 15 amino acids. When corrected for metabolic rate, the R
d values obtained for trout for most amino acids were largely comparable to human values, with the exception of glutamine (which
was lower) and threonine (which was higher). R
d values ranged from 0.9 μmol 100 g−1 h−1 (lysine) to 22.1 μmol 100 g−1 h−1 (threonine) with most values falling between 2 and 6 μmol 100 g−1 h−1. There was a significant correlation between R
d and the molar proportion of amino acids in rainbow trout whole body protein hydrolysate. Other kinetic parameters did not
correlate significantly with whole body amino acid composition. This indicates that an important design feature of the plasma-free
amino acids system involves proportional delivery of amino acids to tissues for protein synthesis. 相似文献
11.
We studied the effects of trace elements, Mn, Mo, and Si, on vasoconstriction induced by norepinephrine (NE) or electrical
field stimulation in isolated porcine right coronary arteries. α1-Adrenoceptor (AR) antagonist prazosin dose-despondently suppressed vasoconstriction in response to NE or field stimulation
indicating an α1-AR mediated response. Mn, Mo, and Si at 0.3-3 μmol/L dosedespondently inhibited NE mediated contraction (allp < 0.05). In contrast, Mn, Mo, and Si at the same concentrations (0.3-3 μmol/L) enhanced the maximal contractile response to
field stimulation in a dose-dependent manner (allp < 0.05), but these elements at 10 μmol/L suppressed the vasoconstrictive response. The results indicate that in porcine right
coronary arteries, the α1-AR-mediated vasoconstriction by NE or electrical field stimulation was affected differently by micromolar concentrations
of Mn, Mo, and Si and that the elements might facilitate NE release presynaptically but inhibit the contractile response postsynaptically. 相似文献
12.
In a previous study, we characterized Cd–Hg interactions for uptake in human intestinal Caco-2 cells. We pursued our investigations
on metal uptake from metal mixtures, focusing on the effects of Hg on cellular homeostasis. A 4-fold higher equilibrium accumulation
value of 0.3 μmol/L 203Hg was measured in the presence of 100 μmol/L unlabeled Hg in the serum-free exposure medium without modification in the initial
uptake rate. This phenomenon was eliminated at 4∘C. Mercury induced an increase in tritiated water and [3H]mannitol uptakes for exposure times greater than 20 min. Incubations for 20 min and 30 min with 100 μmol/L Hg and 2 mmol/L
N-ethylmaleimide (NEM) resulted in a 34% and 50% reductions in cellular thiol staining, respectively, with additive effects.
Lactate dehydrogenase leakage and live/dead assays confirmed the maintenance of cell membrane integrity in Hg- or NEM-treated
cells. We conclude that Hg may alter membrane permeability and increase cell volume without any loss in cell viability. This
phenomenon is sensitive to temperature and could involve Hg interaction with membrane thiols, possibly related to solute transport.
During metal uptake from metal mixtures, Hg may thus promote the uptake of other toxic metals by increasing cell volume and
consequently cell capacity.
Deceased 25 March 2004 相似文献
13.
The concentrations of cadmium, lead, selenium, and zinc in blood and seminal plasma were determined in 76 Singapore males.
Except for zinc, the concentrations were generally higher in blood than in seminal plasma (cadmium, 1.31 μg/L vs 0.61 μg/L;
lead, 82.6 μg/L vs 12.4 μg/L, and selenium, 163.6 μg/L vs 71.5 μg/L). The mean concentration of zinc in seminal plasma was
more than 30 times higher than in blood (202 mg/L vs 6.2 mg/L). Significant positive correlations were found between the concentrations
in blood and seminal plasma for the two essential trace elements: selenium (r=0.45,p<0.001) and zinc (r=0.25,p<0.05). However, no relationships were found between the concentrations in blood and seminal plasma for two toxic metals (cadmium
and lead). Significant inverse correlations were observed between Cd and Zn (r=−0.40,p<0.01), and Pb and Se (r=−0.32,p<0.05) in blood, whereas significant positive correlations were noted between Cd and Se (r=0.45,p<0.01), Cd and Zn (r=0.35,p<0.05), and Se and Zn (r=0.57,p<0.001) in seminal plasma. The physiological significance of these relationships are also discussed in this paper. 相似文献
14.
Leiter E Emri T Gyémánt G Nagy I Pócsi I Winkelmann G Pócsi I 《Folia microbiologica》2001,46(2):127-132
Late-exponential-phasePenicillium chrysogenum mycelia grown in a complex medium possessed an intracellular iron concentration of 650 μmol/L (2.2±0.6 μmol per g mycelial
dry mass). This iron reserve was sufficient to ensure growth and antibiotic production after transferring mycelia into a defined
low-iron minimal medium. Although the addition of Fe3+ to the Fe-limited cultures increased significantly the intracellular iron levels the surplus iron did not influence the production
of penicillin V. Supplements of purified majorP. chrysogenum siderophores (coprogen and ferrichrome) into the fermentation media did not affect the β-lactam production and intracellular
iron level. Neither 150 nor 300 μmol/L extracellular Fe3+ concentrations disturbed the glutathione metabolism of the fungus, and increased the oxidative stress caused by 700 mmol/L
H2O2. Nevertheless, when iron was applied in the FeII oxidation state the oxidative cell injuries caused by the peroxide were significantly enhanced. 相似文献
15.
Eberhard Kauf Horst Dawczynski Gerhard Jahreis Evelyn Janitzky Klaus Winnefeld 《Biological trace element research》1994,40(3):247-253
The effectiveness of a peroral sodium selenite therapy (115 μg Se/m2 BSA/d) administered to cystic fibrosis patients (n=32) could after three months be identified in a significant serum selenium increase (0.69→0.96 μmol/L), a significant malondialdehyde
decrease (2.72→1.64 μmol/L), as well as in a significant serum vitamin E increase (4.31→5.72 μg/mL) Parallel to that, a serum
T3 increase as well as a highly significant decrease in the serum T4/T3-ratio were found, too, which point to improved peripheral T4→T3 conversion during selenium medication. Type-I-iodothyronine-5′-deiodinase has recently been identified as a specific selenoenzyme.
In the case of congenital hypothyroidism (n=37) application of sodium selenite in the above specified dosage yielded a mean serum selenium increase (0.87→1.12 μmol/L),
a not significant T3 increase (2.57→2.61 nmol/L) as well as a not significant TSH decrease (5.34→4.49 mIU/L) without an expected T4 decrease. With the serum lipids, however, a lowering of total cholesterol (4.85→4.53 mmol/L) simultaneous with a mean increase
in HDL-cholesterol (1.52→1.66 mmol/L) as well as a decrease in LDL-cholesterol (2.93→2.52) could be observed. We view the
reduction of the atherogenic serum lipid constellation in the course of selenium medication as an expression of increased
thyroid-hormone efficacy. 相似文献
16.
Hee Y. Paik Hyojee Joung Joo Y. Lee Hong K. Lee Janet C. King Carl L. Keen 《Biological trace element research》1999,69(1):45-57
The present study focused on whether serum extracellular superoxide dimutase (EC-SOD) activity can be used as a functional
indicator of marginal zinc deficiency in humans. Subjects in this study were 444 healthy adults over 30 yr of age living a
normal rural life in Kyunggi province, Korea. The mean dietary zinc intake of subjects obtained from one 24-h recall was 6.41
± 4.35 mg and the average serum zinc concentration of the subjects was 11.06 ± 2.44 (μmol/L. Subjects were divided into three
groups by serum zinc concentrations: adequate (serum zinc >10.7 (μmol/L), low (serum zinc 9.0–10.7 μmol/L), and very low (serum
zinc <9.0 μmol/L) groups. A total of 50 subjects were selected from the three groups for analysis of EC-SOD activities. The
EC-SOD activity of subjects increased with increasing serum zinc concentrations, and the activities of the three groups were
significantly different as indicated by the Kruskal-Wallis test (p = 0.0239). Also, serum EC-SOD activities were significantly
correlated with serum zinc concentrations (r = 0.289,p = 0.04). Serum EC-SOD activities, however, were not significantly correlated to the dietary zinc intakes. In conclusion,
these results show that EC-SOD activities are decreased in subjects with low serum zinc concentrations and suggest that EC-SOD
activity may be a functional indicator of zinc nutritional status in humans. 相似文献
17.
There is substantial information concerning the effects of continuous exposure to supratherapeutic or therapeutic concentrations
of doxorubicin on human molar pulpal cells; the effects of continuous exposure to subtherapeutic concentrations of this agent
are undetermined. To this end, we studied the proliferation of human fibroblasts and pulpal cells and their pattern of mineralized
nodule deposition in vitro. Cell proliferation was assessed at 1, 3, 5, and 7 days from populations with either no exposure (control) or exposure to
10−6–10−9 mol/L doxorubicin. Mineralized nodule deposition and calcium-45 incorporation were assessed at 7 and 21 days of culture.
Data were compared by factorial ANOVA and a post-hoc Tukey test. 10−6 and 10−7 mol/L doxorubicin significantly reduced the total number of viable pulpal cells in cultures from days 1 to 3 (p < 0.05); doxorubicin 10−6–10−9 mol/L significantly inhibited cell proliferation (p < 0.05) and DNA synthesis 5 days after plating (p < 0.001). After 21 days, doxorubicin 10−6–10−8 mol/L significantly decreased calcium-45 incorporation into pulpal cultures (p < 0.001); all dilutions significantly reduced the number of mineralized nodules within the 21-day pulpal cultures (p < 0.05). In addition, all dilutions of doxorubicin significantly inhibited fibroblast cell proliferation and incorporation
of [3H]thymidine. In contrast, the fibroblast cultures did not produce mineralized nodules, suggesting that the mineralized nodules
within the pulpal cell cultures did not result from dystrophic calcification. Thus, exposure to subtheraputic doxorubicin
concentrations has potential adverse effects on mineralized tissue formation within the pulp, which could affect the rates
of reparative dentin deposition within the tooth pulps of patients receiving this chemotherapeutic agent. 相似文献
18.
Eicosapentaenoic acid (EPA) is one of the major dietary polyunsaturated fatty acids and induces apoptosis in several cancer
cells. In this study, the EPA induced lipid peroxidation and response of antioxidative enzymes have been investigated in rat
pheochromocytoma PC12 cells to elucidate the mechanisms of apoptosis induced by the polyunsaturated fatty acid EPA. We have
analyzed superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and glutathione (GSH) contents
in PC12 cells after exposure to different concentrations of EPA. Lipid peroxidation was shown to increase in the presence
of EPA as an indication of the oxidative damage. Lipid peroxidation was enhanced by EPA in a dose-dependent manner, and the
loss of cell viability was partially reversed by vitamin E. In the case of antioxidant enzyme activities, SOD and GPX activities
and GSH contents increased significantly at 50 μmol/L EPA and were respectively 2.41-fold (p < 0.01), 3.49-fold (p < 0.05), and 1.43-fold (p < 0.05) higher than controls. The CAT activity at 10 μmol/L had the highest value and was increased by 25.83% (p < 0.05) compared to control. The results suggest that in PC12 cells the mechanism of apoptosis induced by EPA may be partly
due to lipid peroxidation. 相似文献
19.
Abdellatif Chakar Ridha Mokni Philip A. Walravens Philippe Chappuis Fanny Bleiberg-Daniel Jean-Louis Mahu Daniel Lemonnier 《Biological trace element research》1993,38(1):97-106
Plasma zinc, copper, and parameters of growth were measured in a group of 116 French preschool children, 2–5 yr-old from low-income
households. Participants were selected on the basis of Z-scores of weight for height (WHZ) and height for age (HAZ). Zinc
and copper concentrations of children with growth impairment (GI), defined by a WHZ and/or HAZ< −1 Z-score, were compared
to those of age, sex, and ethnic origin matched controls (WHZ and HAZ >−1 Z-score). Mean (±SD) plasma zinc concentration was
12.58±1.84 μmol/L in the GI group, and 13.27±1.98 μmol/L in the controls. The difference of the means of paired samples was
0.69±2.34, and by pairedt-test the significance reachedp=0.028. This effect was primarily a result of the weight retarded group (WHZ <−1 Z-score,p<0.009) and to the girls (p<0.05). There were no significant differences in plasma copper concentrations between groups. These results suggest the presence
of marginal zinc deficiency in French preschool children with low weight for height Z-scores. 相似文献
20.
Kedzierska K Bober J Ciechanowski K Gołembiewska E Kwiatkowska E Noceń I Dołegowska B Dutkiewicz G Chlubek D 《Biological trace element research》2005,107(1):21-32
The aim of the study was to verify the hypothesis if copper could influence the activity of sodium-transporting systems in
erythrocyte membrane that could be related to essential hypertension. The examined group of patients consisted of 15 men with
hypertension. The control group was 11 healthy male volunteers. The Na+/H+ exchanger (NHE) activity in erythrocytes was determined according to Orlov et al. The activity of transporting systems (ATP-Na+/K+; co-Na+/K+/Cl−; ex-Na+/Li+; free Na+ and K+ outflow [Na+, K+-outflow]) was determined according to Garay's method. The concentration of copper in plasma was assessed using atomic absorption
spectrometry. The activity of ATP-Na+/K+ (μmol/L red blood cells [RBCs]/h) in hypertensive patients was 2231.5±657.6 vs 1750.5±291 in the control (p<0.05), the activity of co-Na+/K+/Cl− (μmol/L RBCs/h) in hypertensives was 171.3±77.9 vs 150.7±53.9 in the control (NS). Na+-outflow (μmol/L RBCs/h) in hypertensives was 118.3±51.6 vs 113.3±24.4 in the control (NS). The K+-outflow (μmol/L RBCs/h) in hypertensives was 1361.7±545.4 vs 1035.6±188.3 in the control (NS). The activity of ex-Na+/Li+ (μmol/L RBCs/h) in hypertensive patients was 266.1±76.1 vs 204.1±71.6 in the control (p<0.05). NHE activity (mmol/L RBCs/h) in hypertensives was 9.7±2.96 vs 7.7±1.33 in the control (p<0.05). In hypertensive patients, negative correlation was found between the activity of Na+/K+/Cl− co-transport and plasma copper concentration (R
s=−0.579, p <0.05) and between the activity of ex-Na+/Li+ and plasma copper concentration (R
s=−0.508, p<0.05). Plasma copper concentration significantly influences the activity of sodium transporting systems in erythrocyte membrane.
Copper supplementation could be expected to provide therapeutic benefits for hypertensive patients. 相似文献