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R E Kiernan C Vanhulle L Schiltz E Adam H Xiao F Maudoux C Calomme A Burny Y Nakatani K T Jeang M Benkirane C Van Lint 《The EMBO journal》1999,18(21):6106-6118
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Regulation of embryonic/fetal globin genes by nuclear hormone receptors: a novel perspective on hemoglobin switching. 总被引:9,自引:1,他引:9
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A Filipe Q Li S Deveaux I Godin P H Roméo G Stamatoyannopoulos V Mignotte 《The EMBO journal》1999,18(3):687-697
The CCAAT box is one of the conserved motifs found in globin promoters. It binds the CP1 protein. We noticed that the CCAAT-box region of embryonic/fetal, but not adult, globin promoters also contains one or two direct repeats of a short motif analogous to DR-1 binding sites for non-steroid nuclear hormone receptors. We show that a complex previously named NF-E3 binds to these repeats. In transgenic mice, destruction of the CCAAT motif within the human epsilon-globin promoter leads to substantial reduction in epsilon expression in embryonic erythroid cells, indicating that CP1 activates epsilon expression; in contrast, destruction of the DR-1 elements yields striking epsilon expression in definitive erythropoiesis, indicating that the NF-E3 complex acts as a developmental repressor of the epsilon gene. We also show that NF-E3 is immunologically related to COUP-TF orphan nuclear receptors. One of these, COUP-TF II, is expressed in embryonic/fetal erythroid cell lines, murine yolk sac, intra-embryonic splanchnopleura and fetal liver. In addition, the structure and abundance of NF-E3/COUP-TF complexes vary during fetal liver development. These results elucidate the structure as well as the role of NF-E3 in globin gene expression and provide evidence that nuclear hormone receptors are involved in the control of globin gene switching. 相似文献
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V Mignotte S Navarro J F Eleouet L I Zon P H Romeo 《The Journal of biological chemistry》1990,265(36):22090-22092
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