共查询到20条相似文献,搜索用时 31 毫秒
1.
Silvian Shama Chi-Yung Lai Jill M. Antoniazzi James C. Jiang S.Michal Jazwinski 《Experimental cell research》1998,245(2):379
The yeastSaccharomyces cerevisiaehas a limited life span that can be measured by the number of times individual cells divide. Several genetic manipulations have been shown to prolong the yeast life span. However, environmental effects that extend longevity have been largely ignored. We have found that mild, nonlethal heat stress extended yeast life span when it was administered transiently early in life. The increased longevity was due to a reduction in the mortality rate that persisted over many cell divisions (generations) but was not permanent. The genesRAS1andRAS2were necessary to observe this effect of heat stress. TheRAS2gene is consistently required for maintenance of life span when heat stress is chronic or in its extension when heat stress is transient or absent altogether.RAS1,on the other hand, appears to have a role in signaling life extension induced by transient, mild heat stress, which is distinct from its life-span-curtailing effect in the absence of stress and its lack of involvement in the response to chronic heat stress. This distinction between theRASgenes may be partially related to their different effects on growth-promoting genes and stress-responsive genes. Theras2mutation clearly hindered resumption of growth and recovery from stress, while theras1mutation did not. TheHSP104gene, which is largely responsible for induced thermotolerance in yeast, was necessary for life extension induced by transient heat stress. An interaction between mitochondrial petite mutations and heat stress was found, suggesting that mitochondria may be necessary for life extension by transient heat stress. The results raise the possibility that theRASgenes and mitochondria may play a role in the epigenetic inheritance of reduced mortality rate afforded by transient, mild heat stress. 相似文献
2.
Silvian Shama Paul A. Kirchman James C. Jiang S.Michal Jazwinski 《Experimental cell research》1998,245(2):368
The replicative life span ofSaccharomyces cerevisiaewas previously shown to be modulated by the homologous signal transducers Ras1p and Ras2p in a reciprocal manner. We have used thermal stress as a life span modulator in order to uncover functional differences between theRASgenes that may contribute to their divergent effects on life span. Chronic exposure of cells throughout life to recurring heat shocks at sublethal temperatures decreased their replicative life span.ras2mutants, however, suffered the largest decrease compared to wild-type andras1mutant cells. The decrease was correlated with a substantial delay in resumption of budding upon recovery from these heat shocks, indicating an impaired renewal of cell cycling. Detailed analysis of gene expression showed that, during recovery,ras2mutants were selectively impaired in down-regulation of stress-responsive genes and up-regulation of growth-promoting genes. Our results suggest that one of the functions ofRAS2in maintaining life span, for whichRAS1does not substitute, is to ensure renewal of growth and cell division after bouts of stress that cells encounter during their life. This activity ofRAS2is effected by the cyclic AMP pathway. Overexpression ofRAS2,but notRAS2ser42which is deficient in the activation of adenylate cyclase, completely reversed the effect of chronic stress on life span. Thus,RAS2is limiting for longevity in the face of chronic stress. SinceRAS2is known to down-regulate stress responses, this demonstrates that for longevity the ability to recover from stress is at least as important as the ability to mount a stress response. 相似文献
3.
Calorie restriction (CR) in microorganisms such as budding and fission yeasts has a robust and well-documented impact on longevity.
In order to efficiently utilize the limited energy during CR, these organisms shift from primarily fermentative metabolism
to mitochondrial respiration. Respiration activates certain conserved longevity factors such as sirtuins and is associated
with widespread physiological changes that contribute to increased survival. However, the importance of respiration during
CR-mediated longevity has remained controversial. The emergence of several novel metabolically distinct microbial models for
longevity has enabled CR to be studied from new perspectives. The majority of CR and life span studies have been conducted
in the primarily fermentative Crabtree-positive yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, but studies in primarily respiratory Crabtree-negative yeast and obligate aerobes can offer complementary insight into the
more complex mammalian response to CR. Not only are microorganisms helping characterize a conserved cellular mechanism for
CR-mediated longevity, but they can also directly impact mammalian metabolism as part of the natural gut flora. Here, we discuss
the contributions of microorganisms to our knowledge of CR and longevity at the level of both the cell and the organism. 相似文献
4.
The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV on nine archaeal strains belonging to each of the
two described genera of Thermococcales, Pyrococcus and Thermococcus, were investigated. To test the efficiency of the EMS and UV mutagenesis under a variety of experimental conditions, we chose
Pyrococcus abyssi strain GE5 as a model strain. We observed a strong induced mutagenicity in both cases, since the spontaneous mutation frequency
(expressed as the frequency of resistance to 5-fluoroorotic acid) increased up to 150-fold with EMS and 400-fold with UV,
after mutagen exposure. Although a heterogeneous response to the induced effects caused after EMS and UV exposures was detected
for all the other sulfothermophilic archaea tested, an efficient mutagenicity of Pyrococcus-like isolates GE27, GE23, and GE9 was observed. Optimal procedures described for UV mutagenesis yielded a number of useful
uracil auxotrophic mutant strains of Pyrococcus abyssi.
Received: 2 May 1996 / Accepted: 3 July 1996 相似文献
5.
S M Jazwinski 《Trends in microbiology》1999,7(6):247-252
The genetic analysis of yeast longevity has illuminated the underlying molecular mechanisms of aging that invoke the importance of metabolic regulation, genetic stability and stress resistance in determination of life span. The RAS genes have emerged as important modulators of life-maintenance processes and of life span itself. 相似文献
6.
《Autophagy》2013,9(10):1702-1711
In response to starvation, cells undergo increased levels of autophagy and cell cycle arrest but the role of autophagy in starvation-induced cell cycle arrest is not fully understood. Here we show that autophagy genes regulate cell cycle arrest in the budding yeast Saccharomyces cerevisiae during nitrogen starvation. While exponentially growing wild-type yeasts preferentially arrest in G1/G0 in response to starvation, yeasts carrying null mutations in autophagy genes show a significantly higher percentage of cells in G2/M. In these autophagy-deficient yeast strains, starvation elicits physiological properties associated with quiescence, such as Snf1 activation, glycogen and trehalose accumulation as well as heat-shock resistance. However, while nutrient-starved wild-type yeasts finish the G2/M transition and arrest in G1/G0, autophagy-deficient yeasts arrest in telophase. Our results suggest that autophagy is crucial for mitotic exit during starvation and appropriate entry into a G1/G0 quiescent state. 相似文献
7.
Prolongation of the yeast life span by the v-Ha-RAS oncogene 总被引:1,自引:0,他引:1
The budding yeast Saccharomyces cerevisiae has a finite life span that is defined by the number of times the cell divides. The patterns of expression of certain genes change in a specific manner during the life span, implying that at least some of the manifestations of the ageing process are subject to gene regulation. It has now been determined that the controlled expression of the RAS oncogene in yeast increases the longevity of this organism, indicating that, conversely, a defined alteration in the activity of a single gene can extend this organism's life span. The results suggest that there is a balance between life-span extension and growth arrest when RAS is expressed. Inasmuch as the homologues of RAS in yeast function to integrate cell metabolism with the cell cycle, these studies raise the possibility that this integrative function may also apply to the co-ordination of successive cell cycles during the life span. 相似文献
8.
Life span extension by targeting a link between metabolism and histone acetylation in Drosophila
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Shahaf Peleg Christian Feller Ignasi Forne Evelyn Schiller Daniel C Sévin Tamas Schauer Catherine Regnard Tobias Straub Matthias Prestel Caroline Klima Melanie Schmitt Nogueira Lore Becker Thomas Klopstock Uwe Sauer Peter B Becker Andreas G Ladurner 《EMBO reports》2016,17(3):455-469
9.
Paul G. Becher Arne Hagman Vasiliki Verschut Amrita Chakraborty Elżbieta Rozpędowska Sébastien Lebreton Marie Bengtsson Gerhard Flick Peter Witzgall Jure Piškur 《Ecology and evolution》2018,8(5):2962-2974
Yeast volatiles attract insects, which apparently is of mutual benefit, for both yeasts and insects. However, it is unknown whether biosynthesis of metabolites that attract insects is a basic and general trait, or if it is specific for yeasts that live in close association with insects. Our goal was to study chemical insect attractants produced by yeasts that span more than 250 million years of evolutionary history and vastly differ in their metabolism and lifestyle. We bioassayed attraction of the vinegar fly Drosophila melanogaster to odors of phylogenetically and ecologically distinct yeasts grown under controlled conditions. Baker's yeast Saccharomyces cerevisiae, the insect‐associated species Candida californica, Pichia kluyveri and Metschnikowia andauensis, wine yeast Dekkera bruxellensis, milk yeast Kluyveromyces lactis, the vertebrate pathogens Candida albicans and Candida glabrata, and oleophilic Yarrowia lipolytica were screened for fly attraction in a wind tunnel. Yeast headspace was chemically analyzed, and co‐occurrence of insect attractants in yeasts and flowering plants was investigated through a database search. In yeasts with known genomes, we investigated the occurrence of genes involved in the synthesis of key aroma compounds. Flies were attracted to all nine yeasts studied. The behavioral response to baker's yeast was independent of its growth stage. In addition to Drosophila, we tested the basal hexapod Folsomia candida (Collembola) in a Y‐tube assay to the most ancient yeast, Y. lipolytica, which proved that early yeast signals also function on clades older than neopteran insects. Behavioral and chemical data and a search for selected genes of volatile metabolites underline that biosynthesis of chemical signals is found throughout the yeast clade and has been conserved during the evolution of yeast lifestyles. Literature and database reviews corroborate that yeast signals mediate mutualistic interactions between insects and yeasts. Moreover, volatiles emitted by yeasts are commonly found also in flowers and attract many insect species. The collective evidence suggests that the release of volatile signals by yeasts is a widespread and phylogenetically ancient trait, and that insect–yeast communication evolved prior to the emergence of flowering plants. Co‐occurrence of the same attractant signals in yeast and flowers suggests that yeast‐insect communication may have contributed to the evolution of insect‐mediated pollination in flowers. 相似文献
10.
Oliveira GA Tahara EB Gombert AK Barros MH Kowaltowski AJ 《Journal of bioenergetics and biomembranes》2008,40(4):381-388
Calorie restriction is a dietary regimen capable of extending life span in a variety of multicellular organisms. A yeast model
of calorie restriction has been developed in which limiting the concentration of glucose in the growth media of Saccharomyces cerevisiae leads to enhanced replicative and chronological longevity. Since S. cerevisiae are Crabtree-positive cells that present repression of aerobic catabolism when grown in high glucose concentrations, we investigated
if this phenomenon participates in life span regulation in yeast. S. cerevisiae only exhibited an increase in chronological life span when incubated in limited concentrations of glucose. Limitation of
galactose, raffinose or glycerol plus ethanol as substrates did not enhance life span. Furthermore, in Kluyveromyces lactis, a Crabtree-negative yeast, glucose limitation did not promote an enhancement of respiratory capacity nor a decrease in reactive
oxygen species formation, as is characteristic of conditions of caloric restriction in S. cerevisiae. In addition, K. lactis did not present an increase in longevity when incubated in lower glucose concentrations. Altogether, our results indicate
that release from repression of aerobic catabolism is essential for the beneficial effects of glucose limitation in the yeast
calorie restriction model. Potential parallels between these changes in yeast and hormonal regulation of respiratory rates
in animals are discussed.
G. A. Oliveira and E. B. Tahara contributed equally to this work. 相似文献
11.
Carlos Alberto Tuão Gava Ana Paula Carvalho de Castro Carliana Araújo Pereira Josélia Santana Gonçalves Ludmilla Ferreira Cajuhi Araújo Cristiane Domingos da Paz 《Biocontrol Science and Technology》2018,28(9):811-822
Post-harvest mango decay is caused by multiple pathogens in tropical conditions but concerns regarding the risk of food contamination by fungicides established biocontrol as a promising alternative. However, occurrence of quiescent infections requires pre-harvest applications of biocontrol agents (BCA), exposing them to harmful UV radiation effects. The objective of this work was to evaluate UV sensitivity of yeast BCA strains previously selected against multiple pathogens that cause mango decay and evaluate suitable UV protectants. In a first bioassay conducted exposing yeast suspensions sprayed on glass plates, it was verified that Saccharomyces sp. ESA47 and Pichia kudriavzevii CMIAT171 were highly sensitive to UV, while Saccharomyces cerevisiae ESA45 had a slightly lower mortality. A bioassay using fragments of mango peels evaluated UV protection from increasing concentrations of starch, dextrin, casein, benzophenone, and cinnamic acid derivative compounds. Results showed that starch and isoamyl p-methoxycinnamate (NHE-1000) resulted in higher survival for yeast strains in doses of 10.0 and 1.0?g?kg?1, respectively. Application of the yeast BCA in a semi-commercial mango orchard resulted in a significant reduction of post-harvest disease incidence and severity. Field application of the yeasts in technical grade preparations containing both UV protectants enhanced the control efficiency by 52.5, 31.9, and 37.7% for ESA45, ESA47, and CMIAT171, respectively. 相似文献
12.
Summary Treatment of diploid yeast cultures with the amino acid analogue, para-fluorophenylalanine (PFPA), at concentrations which caused inhibition of growth, resulted in up to 5 fold increases in the frequency of mitotic gene conversion at two different heteroallelic loci. With haploid yeast cultures, growth in PFPA increased the rate of forward mutation to canavanine resistance by at least 2 fold.Growth of diploids in PFPA prior to exposure to the deaminating agent nitrous acid, the cross-linking agent mitomycin C, the alkylating chemical ethylmethanesulphonate (EMS) and UV light resulted in significant changes in the potency of these diverse mutagens to induce intragenic recombination. For all four mutagens, increased frequencies of gene convertants/viable cell were observed in those cultures which had been exposed to the amino acid analogue prior to mutagen treatment. In haploid WT yeast cells, amino acid analogue incorporation resulted in an enhanced frequency of UV induced forward mutation to canavanine resistance whilst in a DNA repair deficient rad 6 mutant this interaction between UV and PFPA was abolished.The results have been interpreted on the basis of incorporation of the analogue into enzymes involved with DNA replication with a consequent loss of fidelity of such enzymes and increased errors in base incorporation. 相似文献
13.
Aziz A. Khazaeli James W. Curtsinger 《Evolution; international journal of organic evolution》2010,64(12):3409-3416
We measured age‐specific fecundity and survival in recombinant inbred lines of Drosophila melanogaster that were derived from an artificial selection experiment for delayed reproduction. Age at peak oviposition is highly heritable (h2B= 0.55). We find three qualitative categories of peak oviposition: early‐, midlife‐, and bimodal. Genetic correlations between life span and early fecundity are not significantly different from zero, but correlations with midlife fecundity are positive and statistically significant. Long‐lived genotypes exhibit a midlife fecundity peak. There is no evidence for a shift of reproductive effort from early to later stages. The existence of qualitatively recombinant phenotypes, including “superflies” that exhibit both enhanced survival and high levels of early fecundity, argues against the widespread idea that life history evolution in Drosophila is dominated by negative pleiotropy. There is clear evidence of age‐specific adaptation in the timing of oviposition. 相似文献
14.
Kunicka-Styczyńska A Rajkowska K 《World journal of microbiology & biotechnology》2012,28(5):1929-1940
The aim of this study was to examine the physiological and genetic stability of the industrial wine yeasts Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum under acidic stress during fermentation. The yeasts were sub-cultured in aerobic or fermentative conditions in media with
or without l-malic acid. Changes in the biochemical profiles, karyotypes, and mitochondrial DNA profiles were assessed after minimum 50
generations. All yeast segregates showed a tendency to increase the range of compounds used as sole carbon sources. The wild
strains and their segregates were aneuploidal or diploidal. One of the four strains of S. cerevisiae did not reveal any changes in the electrophoretic profiles of chromosomal and mitochondrial DNA, irrespective of culture
conditions. The extent of genomic changes in the other yeasts was strain-dependent. In the karyotypes of the segregates, the
loss of up to 2 and the appearance up to 3 bands was noted. The changes in their mtDNA patterns were much broader, reaching
5 missing and 10 additional bands. The only exception was S. bayanus var. uvarum Y.00779, characterized by significantly greater genome plasticity only under fermentative stress. Changes in karyotypes and
mtDNA profiles prove that fermentative stress is the main driving force of the adaptive evolution of the yeasts. l-malic acid does not influence the extent of genomic changes and the resistance of wine yeasts exhibiting increased demalication
activity to acidic stress is rather related to their ability to decompose this acid. The phenotypic changes in segregates,
which were found even in yeasts that did not reveal deviations in their DNA profiles, show that phenotypic characterization
may be misleading in wine yeast identification. Because of yeast gross genomic diversity, karyotyping even though it does
not seem to be a good discriminative tool, can be useful in determining the stability of wine yeasts. Restriction analysis
of mitochondrial DNA appears to be a more sensitive method allowing for an early detection of genotypic changes in yeasts.
Thus, if both of these methods are applied, it is possible to conduct the quick routine assessment of wine yeast stability
in pure culture collections depositing industrial strains. 相似文献
15.
Thermal environments can influence many fitness‐related traits including life span. Here, we assess whether longevity in Drosophila melanogaster can experimentally evolve as a correlated response to cold‐stress selection, and whether genotype‐by‐temperature and sex‐by‐temperature interactions are significant components of variation in life span. Three replicated S lines were cold‐stress selected and compared with their respective unselected controls (Clines) in the 16th generation of thermal selection. Cold‐stress resistance exhibited a substantial direct response to selection, and also showed a significant interaction between sex and type of line. Mean longevity exhibited a significant interaction between adult test temperature (14 and 25 °C) and line (with suggestive evidence for increased longevity of S lines when tested at 14 °C), but there was no evidence for increased longevity in S lines at normal temperatures (i.e. 25 °C). Another temperature‐dependent effect was sex‐specific, with males being the longer lived sex at 25 °C but the less long‐lived sex at 14 °C. Additionally, we tested in an exploratory way the relationship between longevity and cold‐stress resistance by also measuring resistance to a prefreezing temperature before and after one generation of longevity selection at 14 °C (selection intensity, i = 1.47 for S lines, and 1.42 for C lines). In this longevity selection, we found that cold‐stress resistance increased by about 6% in S lines and 18% in C lines. However, taken together, the results indicate no simple relationship between longevity and cold‐stress resistance, with genotype‐by‐sex interactions in both traits. Temperature dependent interaction in longevity is apparent between S and C lines, and sex‐specific variation in mean longevity also depends on temperature. 相似文献
16.
The biochemical properties of yeasts isolated from sugary substrates such as nectar, plam wine and sugar cane and identified as strains of Saccharomyces carlsbergensis and Saccharomyces cerevisiae were compared with those of imported industrial yeasts. The results presented here show that local yeasts better convert glucose, maltose and sucrose sugars at refrigeration temperature of 8°C than the imported ones. Significant differences existed in the amount of ethanol produced by both, the local and imported yeasts. Whereas the imported brewer's yeast exhibited copper sulphate resistance varying from 3.0mM to 7.0mM, the local isolates gave copper sulphate resistance values ranging from 2.0 to 15.0mM. The local yeast isolates also grew and flocculated faster than the industrial yeasts. The results are discussed in relation to the problems of the brewing industry in Nigeria, a third world's country. 相似文献
17.
Evaluation of different formulations of potential biocontrol yeast isolates efficacy on apple blue mold at storage condition 总被引:1,自引:0,他引:1
L. Mokhtarnejad H. R. Etebarian M. R. Fazeli H. Jamalifar 《Archives Of Phytopathology And Plant Protection》2013,46(10):970-980
Post-harvest pathogens cause major losses in apple production. Biological control by using epiphytic yeasts against Penicillium expansum has been considered as an alternative method for controlling the post-harvest decays. The yeast isolates Rhodotorula mucilaginosa, Pichia guilliermondii, which showed high biocontrol efficacy against P. expansum, were selected for formulation tests. Formulation is an important step in developing a biocontrol product. The successful delivery of biocontrol agents, shelf life, stability and effectiveness in commercial conditions depend on the formulation. In the formulation, the carrier is the primary material used to allow a bioproduct to be dispersed effectively. Yeast isolates were grown in a cane molasses-based medium. Harvested yeast cells were combined with inorganic (talk, kaolin) and organic (Rice bran, wheat bran) carriers. Viability of the yeast cells in formulations stored at 4°C and 24°C was determined each month during 6 months storage. After 6 months storage to evaluate efficacy of formulations, all formulations were tested on apple to control blue mold in storage condition. High stability of antagonistic yeasts was achieved by using organic and inorganic carriers. Rice bran and wheat bran stimulated the germination of the yeasts cells during storage period. Both of the yeasts had significantly highest viable yeast cell content over 6 months in formulation containing wheat bran as a carrier. P.guillermondii in all formulations had significantly higher shelf life and was effective than R. mucilaginosa. 相似文献
18.
Exposure to UV causes a response in yeast and mammalian cells, which is distinct from the response to DNA damage. We report that the mitogen-activated protein kinase Slt2p is involved in this response in Saccharomyces cerevisiae. Thus, budding yeast and mammalian cells respond to UV by using very similar signal transduction pathways. 相似文献
19.
Kyle B. Heine Matthew J. Powers Christine Kallenberg Victoria L. Tucker Wendy R. Hood 《Ecology and evolution》2019,9(17):9759-9767
An important component of life history theory is understanding how natural variation arises in populations. Both endogenous and exogenous factors contribute to organism survival and reproduction, and therefore, it is important to understand how such factors are both beneficial and detrimental to population dynamics. One ecologically relevant factor that influences the life history of aquatic organisms is ultraviolet (UV) radiation. While the majority of research has focused on the potentially detrimental effects that UV radiation has on aquatic organisms, few studies have evaluated hormetic responses stimulated by radiation under select conditions. The goal of this study was to evaluate the impact of UV‐A/B irradiation on life history characteristics in Tigriopus californicus copepods. After exposing copepods to UV‐A/B irradiation (control, 1‐, and 3‐hr UV treatments at 0.5 W/m2), we measured the impact of exposure on fecundity, reproductive effort, and longevity. We found that UV irradiation increased the size of the first clutch among all reproducing females in both the 1‐ and 3‐hr experimental groups and decreased longevity among all females that mated in the 1‐hr treatment. UV irradiation had no effect on the number of clutches females produced. These findings indicate a potential benefit of UV irradiation on reproductive performance early in life, although the same exposure came at a cost to longevity. 相似文献
20.
Summary A number of radiation sensitive mutants of yeast were examined for their sensitivity to the inactivating agents, ultraviolet light (UV), gamma irradiation, ethyl methane sulphonate (EMS) and heat treatment (52° and 37°).A mutant of the gene rad-3, isolated on the basis of its primary sensitivity to UV showed sensitivity only to UV. In contrast the five X-ray sensitive mutants were sensitive to all four inactivating treatments. Considerable variation was observed in the response of the mutants to liquid holding treatment in non-nutrient solution.The data concerning the heat sensitivity of the X-ray sensitive mutants confirms the correlation between heat and X-ray sensitivity observed in bacteria by Bridges (1969).The results indicate that at least two separable pathways of cellular repair exist in yeast, one effective in the repair of UV damage and the other effective in the repair of ionising radiation, alkylating agents, heat and a fraction of UV damage. 相似文献