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1.
Fungi that spoil foods or infect crops can have major socioeconomic impacts, posing threats to food security. The strategies needed to manage these fungi are evolving, given the growing incidence of fungicide resistance, tightening regulations of chemicals use and market trends imposing new food-preservation challenges. For example, alternative methods for crop protection such as RNA-based fungicides, biocontrol, or stimulation of natural plant defences may lessen concerns like environmental toxicity of chemical fungicides. There is renewed focus on natural product preservatives and fungicides, which can bypass regulations for ‘clean label’ food products. These require investment to find effective, safe activities within complex mixtures such as plant extracts. Alternatively, physical measures may be one key for fungal control, such as polymer materials which passively resist attachment and colonization by fungi. Reducing or replacing traditional chlorine treatments (e.g. of post-harvest produce) is desirable to limit formation of disinfection by-products. In addition, the current growth in lower sugar food products can alter metabolic routing of carbon utilization in spoilage yeasts, with implications for efficacy of food preservatives acting via metabolism. The use of preservative or fungicide combinations, while involving more than one chemical, can reduce total chemicals usage where these act synergistically. Such approaches might also help target different subpopulations within heteroresistant fungal populations. These approaches are discussed in the context of current challenges for food preservation, focussing on pre-harvest fungal control, fresh produce and stored food preservation. Several strategies show growing potential for mitigating or reversing the risks posed by fungi in the food supply chain. 相似文献
2.
The importance of copper–ethanolamine-based wood preservatives is increasing. These preservatives usually consist of copper as a fungicide, ethanolamine as a fixative, and secondary fungicides (boron, triazoles) and other additives (water repellents, fixatives, wax emulsions, etc.). Questions arise as to how each of these ingredients interacts with wood-decay fungi, and whether there are any synergistic effects between the components. In order to elucidate these questions, Norway spruce wood specimens were impregnated with five different aqueous solutions consisting of one single component only and of complete formulation of five different concentrations. These specimens were exposed to two brown-rot fungi, Antrodia vaillantii and Gloeophyllum trabeum, as well as to the white-rot fungus Trametes versicolor for 8 weeks according to mini block procedure. In parallel, petri dishes with nutrient medium containing different quantities of ingredients and of complete wood preservative were inoculated with the same fungal species, and their growth was compared with growth on media without chemicals. The results showed that both experimental methods give similar results. In general, there was no synergistic effect determined. Ethanolamine did not decrease fungicidal properties of the system, while on the other hand octanoic acid has a positive effect on the growth of brown-rot fungi. The minimal effective concentration of tested copper–ethanolamine preservative was determined by the minimum effective concentration of the most fungi-toxic ingredient. 相似文献
3.
B. D. L. FITT A. GOULDS T. W. HOLLINS D. R. JONES† 《The Annals of applied biology》1990,117(2):473-486
This review considers strategies for control of eyespot (Pseudocercosporella herpotrichoides) in winter wheat and winter barley in the UK. Varietal resistance to eyespot may take the form of direct resistance to the growth of the pathogen in the stem base or of indirect tolerance to eyespot through resistance to lodging. The French variety Cappelle-Desprez has been a source of resistance for most UK wheat varieties and a new source of resistance in wild goat grass has been discovered recently. Use of fungicides for control of eyespot increased rapidly after the introduction of the highly effective MBC fungicides in the 1970s, but has decreased recently because UK populations of P. herpotrichoides are now predominantly resistant to MBC fungicides and alternative fungicides are more expensive. Historically, cultural methods, especially crop rotation, have been important in the control of eyespot. The importance of cultural control of eyespot is now increasing because more non-cereal break crops are being grown and fungicide use is declining. 相似文献
4.
《Biological Control》2004,29(1):109-114
The Colorado potato beetle is an important pest on potato, eggplant, and tomato. Because Colorado potato beetles develop resistance to insecticides quickly, new methods are needed for control. Bacillus thuringiensis is the only bacterium to successfully control Colorado potato beetle. Until recently, one of the drawbacks to testing bacteria against the Colorado potato beetle has been the lack of an artificial diet for screening. Previous artificial diets will only be consumed by Colorado potato beetle larvae when fresh. To improve storage, we developed a freeze-dried diet, based on a 96-well plate, suitable to feed larvae for the duration of a bioassay. Individual diet components were tested both for their effect on insect growth and on pathogen toxicity. When the preservatives, methylparaben and sorbic acid, were removed from the diet, the average weight of second instar larvae increased from 7.9 mg to greater than 9.8 mg. The preservatives inhibited the growth of two of the bacteria tested, Photorhabdus luminescens HM and Chromobacterium sp. PRAA. The removal of these preservatives also allowed for fungal growth and reduced survival from 94 to 38%. Removing diet preservatives, that inhibited the growth of Chromobacterium sp. PRAA, increased the total mortality of the larvae as well as reducing the time needed to kill 50% of the larvae. Compared to incorporation of bacteria into molten diet, the total mortality of Colorado potato beetle fed either P. luminescens HM or Chromobacterium sp. PRAA on freeze-dried diet doubled. Preparation of freeze-dried diet need not be synchronized with the insect or the pathogen. The freeze-dried diet gave consistent results as measured by low control mortality and pathogen toxicity over time. 相似文献
5.
Rende Qi Tao Wang Wei Zhao Ping Li Jiancheng Ding Zhimou Gao 《Journal of Phytopathology》2012,160(11-12):717-722
Pepper Phytophthora blight (PPB), caused by Phytophthora capsici, is an important disease of pepper in China. The extensive application of metalaxyl has resulted in widespread resistance to this fungicide in field. This study has evaluated the activities of several fungicides against the mycelial growth and sporangium germination of metalaxyl‐sensitive and metalaxyl‐resistant P. capsici isolates by determination of EC50 values. The results showed that the novel carboxylic acid amide (CAA) fungicide mandipropamid exhibited excellent inhibitory activity against PPB both in vitro and in vivo, with averagely EC50 values of 0.075 and 0.004 μg/ml in mycelial growth and sporangium germination, respectively, and over 88% efficacy in controlling PPB. The other three CAA fungicides also provided over 70% efficacy in controlling PPB. The mycelial growth was less sensitive to quinone outside inhibitor (QoI) fungicides azoxystrobin and trifloxystrobin than that of sporangium germination in P. capsici isolates. However, azoxystrobin and trifloxystrobin provided over 80% efficacy in controlling PPB. It was noted that propamocarb and cymoxanil did not exhibit activity against the mycelial growth or sporangium germination of P. capsici isolates in the in vitro tests, with over 70% efficacy in controlling PPB. The new fungicide mixture 62.5 g/l fluopicolide + 625 g/l propamocarb (trade name infinito, 687.5 g/l suspension concentrate (SC)) produced over 88% efficacy in controlling PPB caused by both metalaxyl‐sensitive and metalaxyl‐resistant isolates. The data of this study also proved that there was obviously no cross‐resistance between metalaxyl and the other tested fungicides. Therefore, these fungicides should be good alternatives to metalaxyl for the control of PPB and management of metalaxyl resistance. 相似文献
6.
Phillips CA 《Letters in applied microbiology》1999,29(6):424-428
The importance of Arcobacter spp. as a cause of human foodborne illness is unresolved. Organic acids and their sodium salts, and nisin are preservatives commonly used in the type of foods from which the organism is recovered. In this study their effect on the growth of A. butzleri in culture, alone and in combination, was investigated. At 0.5%, 1.0% and 2.0% lactic and citric acids inhibited A. butzleri growth; 2% sodium lactate was effective in inhibiting growth over 8 h incubation but not over longer periods. Sodium citrate was more effective than sodium lactate. Nisin alone inhibited A. butzleri growth at 500 IU ml-1 over 5 h. It did not enhance the effect of sodium citrate inhibition but it did augment the effect of sodium lactate alone over 8 h. 相似文献
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8.
Weak-acid preservatives are widely used to prevent microbial spoilage of acidic foods and beverages. Characteristically, weak-acid preservatives do not kill micro-organisms but inhibit growth, causing very extended lag phases. Preservatives are more effective at low pH values where solutions contain increased concentrations of undissociated acids. Inhibition by weak-acids involves rapid diffusion of undissociated molecules through the plasma membrane; dissociation of these molecules within cells liberates protons, thus acidifying the cytoplasm and preventing growth. By modelling preservative action in yeast, using a thermodynamic and kinetic approach, it was possible to demonstrate that: (i) inhibition depends more on the degree to which individual preservatives are concentrated within cells, rather than on undissociated acid concentration per se; (ii) it is entirely feasible for microbes to pump protons out of the cell during extended lag phase and raise internal pH (pHi), despite further influx of preservatives; (iii) the duration of the lag phase can be predicted from the model, using a Gaussian fit of proton-pumping H(+)-ATPase activity against pHi; (iv) theoretical ATP consumption for proton pumping can be directly correlated with the reduction in cell yield observed in glucose-limited cultures. 相似文献
9.
The ABC transporter BcatrB affects the sensitivity of Botrytis cinerea to the phytoalexin resveratrol and the fungicide fenpiclonil 总被引:6,自引:0,他引:6
Schoonbeek H Del Sorbo G De Waard MA 《Molecular plant-microbe interactions : MPMI》2001,14(4):562-571
During pathogenesis, fungal pathogens are exposed to a variety of fungitoxic compounds. This may be particularly relevant to Botrytis cinerea, a plant pathogen that has a broad host range and, consequently, is subjected to exposure to many plant defense compounds. In practice, the pathogen is controlled with fungicides belonging to different chemical groups. ATP-binding cassette (ABC) transporters might provide protection against plant defense compounds and fungicides by ATP-driven efflux mechanisms. To test this hypothesis, we cloned BcatrB, an ABC transporter-encoding gene from B. cinerea. This gene encodes a 1,439 amino acid protein with nucleotide binding fold (NBF) and transmembrane (TM) domains in a [NBF-TM6]2 topology. The amino acid sequence has 31 to 67% identity with ABC transporters from various fungi. The expression of BcatrB is up regulated by treatment of B. cinerea germlings with the grapevine phytoalexin resveratrol and the fungicide fenpiclonil. BcatrB replacement mutants are not affected in saprophytic growth on different media but are more sensitive to resveratrol and fenpiclonil than the parental isolate. Furthermore, virulence of deltaBcatrB mutants on grapevine leaves was slightly reduced. These results indicate that BcatrB is a determinant in sensitivity of B. cinerea to plant defense compounds and fungicides. 相似文献
10.
Wojdyła AT 《Communications in agricultural and applied biological sciences》2007,72(4):961-966
The effectiveness of 5 strobilurin fungicides: Acanto 250 SC, Amistar 250 SC, Discus 500 WG, Signum 33 WG, Zato 50 WG and Score 250 EC (triazole-standard) in the control of Puccinia horiana was tested on chrysanthemum cv. Melba Blane. Number of pustules per leaf was observed before treatment, as well as 2 and 4 weeks after treatment. Additionally, after 4 weeks, their influence on plant growth, size of pustules and eventually phytotoxicity were assessed. Plants were sprayed preventively and curatively 4 times at weekly intervals. In preventive programme after 4 weeks of investigations, average of about 1.5 spot per leaf was recorded on control plants. Disease symptoms did not appear on chrysanthemums protected by fungicides Signum 33 WG at concentration 0.18% and Zato 50 WG at concentration 0.015%. In case of the other tested fungicides only sporadically pustules on single leaves were noticed (over 95% of them were browned, dried and crumbled). On control plants more than 46% of leaves was infected but in case of the other investigated fungicides it was only 3-7%. Diameter of spots on plants protected with: Score 250 EC, Acanto 250 SC and Discus 500 WG was similar as on the leaves of control plants. On plants protected with Amistar 250 SC the diameter of pustules was significantly lower than on control plants. In curative programme after 4 weeks of tests, leaves of control plants had about 4 spots per leaf. On leaves sprayed with Score 250 EC at concentration 0.05% there were 4 times less spots than on control plants. Chrysanthemums treated with tested strobilurin fungicides had about 2 times less pustules than on control plants. Diameter of spots on control plant leaves was 3.6 mm and was similar as on chrysanthemums protected with tested fungicides. On leaves of control plants all pustules were active without visible changes or damage but in case of the investigated fungicides almost 100% of them were browned, dried and crumbled. After 4 weeks of experiment, a measured height of control plants was about 125 mm and was similar to the height of plants protected with tested fungicides except Signum 33 WG, which stimulated plant growth. None of tested compounds showed phytotoxic effect. 相似文献
11.
Succinate dehydrogenase inhibitor (SDHI) fungicides are important tools to control preharvest and postharvest brown rot of stone fruits. In this study we determined the mycelial growth rate of Monilinia fructicola isolates on various media and picked the three that allowed the fastest growth to assess the sensitivity of mycelium to SDHI fungicides boscalid, fluopyram, and penthiopyrad. Minimal medium (MM) supported mycelial growth the best and yielded lowest EC50 values for three SDHI fungicides. EC50 values corresponded with disease incidence data obtained from detached fruit assays. Penthiopyrad had significantly greater intrinsic activity in vitro compared to fluopyram at the α = 0.05 level and compared to boscalid at the α = 0.1 level. However, detached fruit assays revealed that this ‘advantage’ did not carry over in vivo. In conclusion, MM appears to be the best medium currently available to assess the sensitivity of M. fructicola mycelium in vitro. 相似文献
12.
Sasaki YF Kawaguchi S Kamaya A Ohshita M Kabasawa K Iwama K Taniguchi K Tsuda S 《Mutation research》2002,519(1-2):103-119
We determined the genotoxicity of 39 chemicals currently in use as food additives. They fell into six categories-dyes, color fixatives and preservatives, preservatives, antioxidants, fungicides, and sweeteners. We tested groups of four male ddY mice once orally with each additive at up to 0.5xLD(50) or the limit dose (2000mg/kg) and performed the comet assay on the glandular stomach, colon, liver, kidney, urinary bladder, lung, brain, and bone marrow 3 and 24h after treatment. Of all the additives, dyes were the most genotoxic. Amaranth, Allura Red, New Coccine, Tartrazine, Erythrosine, Phloxine, and Rose Bengal induced dose-related DNA damage in the glandular stomach, colon, and/or urinary bladder. All seven dyes induced DNA damage in the gastrointestinal organs at a low dose (10 or 100mg/kg). Among them, Amaranth, Allura Red, New Coccine, and Tartrazine induced DNA damage in the colon at close to the acceptable daily intakes (ADIs). Two antioxidants (butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT)), three fungicides (biphenyl, sodium o-phenylphenol, and thiabendazole), and four sweeteners (sodium cyclamate, saccharin, sodium saccharin, and sucralose) also induced DNA damage in gastrointestinal organs. Based on these results, we believe that more extensive assessment of food additives in current use is warranted. 相似文献
13.
Understanding the effect of heavy metals and wood preservatives on the growth of wood-rot fungi native to a certain region
may improve reliability in determining the effectiveness of antifungal products, particularly when dealing with new formulations.
In this investigation, strains of copper-tolerant wood-rot fungi native to south-central Chile were evaluated against two
preservatives: commercial chromated copper arsenate type C (CCA-C) and a new formulation with boron and silicon (BS). Thirteen
native strains, mainly white-rot fungi, were selected for their high growth rates in solid medium containing 3 mM of copper.
A short-term test was then carried out, consisting of adding cellulose disks impregnated with different concentrations of
preservatives to solid culture media inoculated with selected copper tolerant strains. There was a great variability in interspecific
and intraspecific responses to the presence of copper and preservatives in culture media. Among the native and commercial
strains evaluated, the white-rot fungi Trametes versicolor 38 and mainly Ganoderma australe 100 were notable for their tolerance to all the CCA-C and BS concentrations. The brown-rot fungus Wolfiporia cocos, used as reference strain, showed a high tolerance to CCA-C, but not to BS preservative. T. versicolor 38 and G. australe 100 were selected for subsequent studies on preserved wood degradation. 相似文献
14.
At a time when consumers are demanding the partial or complete removal of chemically synthesized preservatives from foods, there is also an increased demand for convenience foods with long shelf-lives. These consumer-led trends have fuelled a renewed interest in the development of ‘more natural’ preservatives for extending the shelf-life and maintaining the safety of foods. Although the antimicrobial properties of many compounds from plant, animal and microbial sources have been reported, their potential for use as natural food preservatives has not been fully exploited. In this paper, the possible uses of natural antimicrobial compounds as food preservatives, used either singly or in combination, are explored. Specific examples are given from a current transnational research project on Natural Antimicrobial Systems sponsored jointly by the European Commission and a consortium of eight food companies. The results of trials with a range of potential natural preservatives including lytic enzymes, bacteriocins from lactic acid bacteria and plant antimicrobials in laboratory media and in a variety of foods and beverages including apple juice, milk, hard-cooked cheese (Emmental) and fresh fruit slices are discussed. 相似文献
15.
Effect of oxathiapiprolin on asexual life stages of Phytophthora capsici and disease development on vegetables
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Phytophthora blight caused by Phytophthora capsici is a serious disease in the production of peppers and other vegetables worldwide. Application of fungicides is an important component in developing effective disease management programmes. However, resistance in P. capsici populations to some commonly used fungicides has been documented. Identification of effective new fungicides with different mode of actions is highly desirable. This study was conducted to determine baseline sensitivity of P. capsici isolates to oxathiapiprolin, the first member of a new class of isoxazoline fungicides, and efficacy of this compound for reduction of Phytophthora blight on bell pepper. A collection of 126 P. capsici isolates were evaluated and all the isolates were sensitive to oxathiapiprolin. EC50 values of oxathiapiprolin in inhibiting mycelial growth, sporangium formation and zoospore germination of 25 selected isolates averaged 0.001, 0.0003 and 0.54 µg mL?1, respectively. It appeared that asexual life stages of P. capsici were more sensitive to oxathiapiprolin than other compounds used for control of oomycete pathogens. In field studies, oxathiapiprolin applied at different rates through drip irrigation tubes, or by soil drench plus foliar sprays, reduced Phytophthora blight and increased pepper yield significantly. This is the first report of the efficacy of oxathiapiprolin in suppression of P. capsici, which indicates that oxathiapiprolin is effective in inhibiting the pathogen and has the promise to be a viable option for managing Phytophthora blight in bell pepper production. 相似文献
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17.
The effects of pesticides on 64 ectomycorrhizal fungi of boreal forest trees were studied in vitro. The pesticides (fungicides: benomyl, chlorothalonil, copper oxychloride, maneb and propiconazole; herbicides: chlorthiamid, glyphosate, hexazinone, linuron and terbuthylazine; insecticide: cypermethrin) were selected as those commonly used in Nordic forest nurseries and afforestation sites. In general, the fungicides proved to be more toxic to ectomycorrhizal fungi than the herbicides and cypermethrin. The fungicides, chlorothalonil and propiconazole, had the clearest inhibitory effect on growth of mycorrhizal fungi. Conversely, maneb, glyphosate and terbuthylazine stimulated the growth of some mycorrhizal fungi. Leccinum versipelle and L. scabrum, Paxillus involutus and Cenococcum geophilum were the most sensitive ectomycorrhizal fungi to the various pesticides. 相似文献
18.
Three fungicides, chlorothalonil, dichloran and mancozeb were studied to determine the effects on growth and production of roridin E by Myrothecium roridum in vitro. With increasing concentrations of fungicides, both growth and production of roridin E were inhibited. Of the three fungicides, chlorothalonil was much more effective in the inhibition of growth and production of roridin E, than dichloran and mancozeb. 相似文献
19.
Creemers P Van Laer S Van Mechelen A Vorstermans B Hauke K 《Communications in agricultural and applied biological sciences》2007,72(4):917-923
As new fungicides are mainly unisite action fungicides, the problem of fungicide resistance development is becoming more important every year. Combining chemical fungicides, which is the best anti-resistance strategy, is not always possible or recommended in the case when the number of available chemical fungicides are limited or a reduction in fungicide use is asked for. Therefore the use of salts as an anti-resistance strategy was looked upon. The salts evaluated were K(HCO3), KH2PO3, KHPO4 and K2SiO3. When using these salts as an anti-resistance strategy the efficacy obtained when spraying the compounds alone was often to low to be used in rotation with chemical fungicides. Only with K(HCO3)2 a good efficacy can be observed in some years. The variation in efficacy with K(HCO3)2 observed is higher for powdery mildew. Chitosan was also included in the trials against powdery mildew, however chitosan had no effect on the infestation. 相似文献
20.
The effect of two herbicides (paraquat and simazine on the antifungal activity of two fungicides (captan and mounsrin) against Rhizoctonia solani was studied. when the herbicides paraquat and simazine were applied to soil they altered the effectiveness of both fungicides in controlling R. solani , thus causing damping-off of cotton. Both herbicides increased the toxicity of both fungicides against mycelial growth of the fungus. In pot tests, seeds or soil treated with captan or mounsrin, gave better control of R. solani damping-off disease when the soil was treated with paraquat or simazine compared to untreated soil. Captan was, however, found to be more effective in controlling the disease than mounsrin. 相似文献