共查询到20条相似文献,搜索用时 31 毫秒
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Ramil N Nurtdinov Alexey D Neverov Alexander V Favorov Andrey A Mironov Mikhail S Gelfand 《BMC evolutionary biology》2007,7(1):249
Background
Alternative splicing has been shown to be one of the major evolutionary mechanisms for protein diversification and proteome expansion, since a considerable fraction of alternative splicing events appears to be species- or lineage-specific. However, most studies were restricted to the analysis of cassette exons in pairs of genomes and did not analyze functionality of the alternative variants. 相似文献4.
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Ganesh S Shankarling Penelope W Coates Brinda Dass Clinton C MacDonald 《BMC molecular biology》2009,10(1):22
Background
Alternative splicing and polyadenylation are important mechanisms for creating the proteomic diversity necessary for the nervous system to fulfill its specialized functions. The contribution of alternative splicing to proteomic diversity in the nervous system has been well documented, whereas the role of alternative polyadenylation in this process is less well understood. Since the CstF-64 polyadenylation protein is known to be an important regulator of tissue-specific polyadenylation, we examined its expression in brain and other organs. 相似文献6.
Jurriaan J Hölzenspies Willem Stoorvogel Ben Colenbrander Bernard AJ Roelen Dagmar R Gutknecht Theo van Haeften 《BMC developmental biology》2009,9(1):1-16
Background
Fibronectin 1 (FN1), a glycoprotein component of the extracellular matrix, exerts different functions during reproductive processes such as fertilisation, gastrulation and implantation. FN1 expression has been described to increase significantly from the morula towards the early blastocyst stage, suggesting that FN1 may also be involved in early blastocyst formation. By alternative splicing at 3 defined regions, different FN1 isoforms are generated, each with a unique biological function. The analysis of the alternative FN1 splicing on the one hand and the search for candidate FN1 receptors on the other hand during early bovine embryo development may reveal more about its function during bovine preimplantation embryo development. 相似文献7.
Background
Alternative splicing of mutually exclusive exons is an important mechanism for increasing protein diversity in eukaryotes. The insect Mhc (myosin heavy chain) gene produces all different muscle myosins as a result of alternative splicing in contrast to most other organisms of the Metazoa lineage, that have a family of muscle genes with each gene coding for a protein specialized for a functional niche. 相似文献8.
Background
Alternative splicing is an efficient mechanism for increasing the variety of functions fulfilled by proteins in a living cell. It has been previously demonstrated that alternatively spliced regions often comprise functionally important and conserved sequence motifs. The objective of this work was to test the hypothesis that alternative splicing is correlated with contact regions of protein-protein interactions. 相似文献9.
Danny A Bitton Michał J Okoniewski Yvonne Connolly Crispin J Miller 《BMC bioinformatics》2008,9(1):118
Background
Previous studies comparing quantitative proteomics and microarray data have generally found poor correspondence between the two. We hypothesised that this might in part be because the different assays were targeting different parts of the expressed genome and might therefore be subjected to confounding effects from processes such as alternative splicing. 相似文献10.
Alternative splicing and protein function 总被引:1,自引:0,他引:1
Background
Alternative splicing is a major mechanism of generating protein diversity in higher eukaryotes. Although at least half, and probably more, of mammalian genes are alternatively spliced, it was not clear, whether the frequency of alternative splicing is the same in different functional categories. The problem is obscured by uneven coverage of genes by ESTs and a large number of artifacts in the EST data. 相似文献11.
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Fan Mo Xu Hong Feng Gao Lin Du Jun Wang Gilbert S Omenn Biaoyang Lin 《BMC bioinformatics》2008,9(1):537
Background
Alternative splicing is an important gene regulation mechanism. It is estimated that about 74% of multi-exon human genes have alternative splicing. High throughput tandem (MS/MS) mass spectrometry provides valuable information for rapidly identifying potentially novel alternatively-spliced protein products from experimental datasets. However, the ability to identify alternative splicing events through tandem mass spectrometry depends on the database against which the spectra are searched. 相似文献13.
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Ramesh Palaniswamy Stephan Teglund Matthias Lauth Peter G Zaphiropoulos Takashi Shimokawa 《BMC molecular biology》2010,11(1):32
Background
Alternative splicing is one of the key mechanisms that generate biological diversity. Even though alternative splicing also occurs in the 5' and 3' untranslated regions (UTRs) of mRNAs, the understanding of the significance and the regulation of these variations is rather limited. 相似文献16.
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Ting-Yu Chang Yin-Yi Li Chih-Hung Jen Tsun-Po Yang Chi-Hung Lin Ming-Ta Hsu Hsei-Wei Wang 《BMC bioinformatics》2008,9(1):432
Background
Alternative RNA splicing greatly increases proteome diversity and thereby contribute to species- or tissue-specific functions. The possibility to study alternative splicing (AS) events on a genomic scale using splicing-sensitive microarrays, including the Affymetrix GeneChip Exon 1.0 ST microarray (exon array), has appeared very recently. However, the application of this new technology is hindered by the lack of free and user-friendly software devoted to these novel platforms. 相似文献18.
Sadayuki Matuda Takuro Arimura Akinori Kimura Hiroaki Takekura Shigeo Ohta Kyoko Nakano 《Biochimica et Biophysica Acta (BBA)/General Subjects》2010
Background
It is not known if the dihydrolipoamide succinyltransferase (DLST) gene, a mitochondrial protein, undergoes alternative splicing. We identified an uncharacterized protein reacting with an anti-DLST antibody in the I bands of myofibrils in rat skeletal muscle.Methods
Immunocytochemical staining with an anti-DLST antibody, the purification and amino acid sequence analysis of the protein, and the isolation and sequencing of the protein's cDNA were carried out to clarify the properties of the protein and its relationship to the DLST gene.Results
A pyrophosphate concentration >10 mM was necessary to extract the protein from myofibrils in the presence of salt with a higher concentration than 0.6 M, at an alkaline pH of 7.5–8.0. The protein corresponded to the amino acid sequence of the C-terminal side of DLST. The cDNAs for this protein were splicing variants of the DLST gene, with deletions of both exons 2 and 3, or only exon 2 or 3. These variants possessed an open reading frame from an initiation codon in exon 8 of the DLST gene to a termination codon in exon 15, generating a protein with a molecular weight of 30 kDa.Conclusions
The DLST gene undergoes alternative splicing, generating the protein isolated from the I bands of myofibrils.General significance
The DLST gene produces two different proteins with quite different functions via alternative splicing. 相似文献19.
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