Sperm morphology of cattle and domestic pigs

Sperm morphology was evaluated (using Blom classification) in 44 ejaculates of 11 bulls and 44 ejaculates of 11 boars. Significant differences in sperm morphology were found between bulls and boars. In addition, the correlations between frequency of morphological changes and morphometrical traits of boar spermatozoa were demonstrated. More morphological anomalies of spermatozoa were recorded in ejaculates containing longer spermatozoa.     

Photoperiod and luteinizing hormone secretion in domestic and wild pigs

During seasonal anoestrus (long-days), oestradiol can effectively inhibit the pulsatile secretion of luteinizing hormone (LH) in sheep. The aim of our trial was to determine whether the same regulatory mechanism exists in the pig. Altogether, 20 ovariectomized and oestradiol-implanted gilts (16 domestic pigs, 4 European wild boars) were randomly allocated to two treatment groups. The first group was kept under a short-day light-dark cycle of 8L:16D, and the second group under a long-day light regime of 16L:8D. After a 6-week treatment period, blood samples were taken at 20-min intervals for 12h. After sampling, the light regimens were switched. Sampling was then repeated following another 6 weeks of treatment. In both treatment groups, 2.3 LH pulses occurred every 12h. The basal LH level was 0.7+/-0.4 ng/ml for the short-day group and 1.0+/-0.5 ng/ml for the long-day group. The mean LH level was 0.9+/-0.4 and 1.3+/-0.6 ng/ml and the LH pulse amplitude 0.5+/-0.4 and 0.6+/-0.5 ng/ml, respectively. The basal and mean LH levels were therefore lower in short-day gilts (P<0.05), while LH pulse amplitude and frequency remained unaffected by treatment. In conclusion, the 6-week period under two different light regimes resulted in higher basal LH concentration in long-day gilts but was not able to produce changes in LH frequency in prepubertal gilts.     

Genealogy, morphology and fitness of spontaneous hybrids between wild and cultivated chicory (Cichorium intybus)

Crop species are known to hybridize spontaneously with wild relatives, but few studies have characterized the performance of hybrids at various genealogies, life stages and environments. A group of cultivar-like individuals and potential hybrids were observed in a roadside population of wild chicory plants in Denmark. Seeds were collected from all reproductive plants and grown in a common garden experiment, and their morphological and genetic compositions were analysed. Intermediate plants were identified as hybrids and comprised various backcross and F(n) combinations. A genotypic hybrid index (HI), spanning from wild-like to cultivar-like, was highly correlated to a morphological index. Plant survival, growth and reproduction were evaluated and compared to the genotypic HI. Overall, cultivar-like and intermediate plants grew larger than wild-like plants, flowered longer, and produced more flowers and seeds. The common garden included a nutrient gradient. At higher nutrient levels, intermediate and cultivar-like plants produced more flowers and seeds than wild-like plants, whereas this effect was less pronounced at lower nutrient levels. During winter, small rodents consumed roots of cultivar-like and intermediate plants preferentially. Thus, cultivated and wild chicory are able to hybridize spontaneously, producing hybrid offspring of several generations that may reproduce more effectively than their wild parent, but herbivory and poor environmental conditions may negatively affect their fitness.     

Structure traits of Longissimus lumborum muscle in wild boar/domestic pig hybrids

The structure of musculus longissimus lumborum (m.l.l.) was evaluated with respect to proportion of three muscle fiber types and their diameters, in gilts and barrows of wild boar/domestic pig hybrids. The experiment was carried out on 29 hybrids (21 barrows and 8 gilts) which originated from crossing Duroc sows with wild boar (Sus scrofa ferus). The diameter of the "red" fibres in barrows was 48.42 microm, significantly greater than in gilts--43.71 microm. The diameters of the remaining two types of fibres in barrows and gilts were similar and amounted to: "intermediate" fibres--39.08 and 40.79 microm, "white" fibres--51.42 and 51.03, respectively. In respect to sex no statistical differences in proportion of any types of fibers were found, which in barrows came to: "red"--12.93%, "intermediate"--30.17% and "white"--57.32%, and in gilts to: 12.50, 27.04 and 60.45%, respectively. In conclusion, in m.l.l. of wild boar/domestic pig hybrids, smaller diameters of all muscle fibers types were identified as well as a higher proportion of intermediate fibers in comparison to m.l.l. of various pig breeds. An increased proportion of the intermediate fibers probably results from the interaction between factors such as age (8-months), physical activity (open-air for running) and the influence of wild boar genes.     

Frost sensitivity of hybrids between wild and cultivated carrots

Frost could potentially limit the survival ofhybrids between cultivated and wild carrots innatural habitats, and thereby the likelihoodthat (trans)genes spread from the crop to wildplants. To test this, cultivated, wild carrotsand their hybrids were exposed to differentfrost treatments. Hybrids survivedsignificantly less than the wild carrots, butonly slightly better than the cultivars,indicating that frost will indeed limit theirsurvival.     

A transferrin variant Tf I in crosses of the wild and domestic pigs

In crosses of the wild pig (Sus scrofa attila Thomas) with the domestic pig a transferrin variant, Tf I, was detected, electrophoretic mobility of which was slightly faster than the mobility of the variant Tf A. From the results of starch gel electrophoresis, isolation, neuraminidase treatment, autoradiography, and genetic analysis of several families, it can be concluded that the Tf I variant is genetically controlled by the allele Tf¹. Thus the number of alleles in the transferrin system of the pig has increased to six ( Tf^I, Tf^ATf^B, Tf^C, TP^D and Tf^E ).     

Variation in mitochondrial DNA of Vietnamese pigs: relationships with Asian domestic pigs and Ryukyu wild boars

Mitochondrial DNA (mtDNA) sequences (574 bp) of 30 Vietnamese pigs (large and small) were examined and compared with those of 61 haplotypes from wild boars and domestic pigs from various locations in Asia. The large Vietnamese pigs had genetic links to Ryukyu wild boars in southern Japan. The small Vietnamese pigs were closely related to other East Asian domestic pigs. These results indicate that Vietnamese pigs are genetically diverse and may be descendents of wild and domestic pigs from other regions of Asia.     

Cytogenetics of F1 hybrids between Cajanus and Atylosia species and its phylogenetic implications

Summary Cytogenetic relationships between two cultivars of Cajanus cajan and six species of Atylosia were investigated. Of the 12 cross combinations obtained, only seven could be studied. Meiotic chromosome pairing, pollen and ovule fertility in parental species and four F1 hybrids were near normal. Some meiotic abnormalities were observed in the Fls: A. lineata x A. scarabaeoides, A. scarabaeoides x A. sericea and C. cajan (UPAS 120) x A. trinervia, indicating varying degrees of chromosomal and genic differences between these species. These observations suggested that A. cajanifolia is the closest wild relative of C. cajan, followed by A. scarabaeoides, A. albicans and A. trinervia. Among the Atylosia species, A. sericea was closer to A. scarabaeoides than to A. lineata.Botanist, Genetic Resources Unit, ICRISAT, Patancheru-502 324, IndiaRegional Plant Production and Protection Officer, FAO, RAPA, Bangkok-2, Thailand     

Cytogenetics of Triticum x Dasypyrum hybrids and derived lines

Genomic in situ hybridization was used to study Triticum x Dasypyrum wide hybrids and derived lines. A cytogenetic investigation was carried out in progenies of (i) amphiploids derived from T. turgidum var. durum (T. durum; 2n = 14; genomes AABB) x D. villosum (2n = 14; genome VV), (ii) three-parental hybrids (T. durum x D. villosum) x T. aestivum (2n = 42, genomes A'A'B'B'D'D'), and (iii) T. aestivum aneuploid lines carrying D. villosum chromosomes or chromatin. The amphiploids derived from T. durum x D. villosum showed a stable chromosomal constitution, made up of 14 V chromosomes, 14 chromosomes carrying the wheat A genome and 14 chromosomes carrying the B genome. High karyological instability was observed in the progenies of three-parental hybrids ([T. durum x D. villosum] x T. aestivum). Plants having the expected 14 A chromosomes, 14 B chromosomes, 7 D chromosomes, and 7 V chromosomes were rather rare (4.5%). Many progeny plants (45.5%) had the hexaploid wheat genome with 42 chromosomes and lacked any detectable D. villosum chromatin. Other plants (50%) had 14 A chromosomes and 14 B chromosomes, plus variable numbers of D and V chromosomes, the former being better retained than the latter in most cases. Some T. aestivum lines carrying D. villosum chromosomes or chromatin, as the result of addition, substitution, or recombination events or even a combination of these karyological events, were found to be stable. Other lines were unstable, and these lines carried 1V, 3V, or 5V chromosomes or their portions. Substitution or recombination events where 1V chromosomes were involved could concern the homeologous counterparts in both the A and B and D genomes of wheat. No line could be recovered where the shorter arm of 3V chromosomes was present. Changes in the morphology and banding pattern of V chromosomes were observed in hybrids that did not carry the entire D. villosum complement. By comparing the results of our cytogenetic analyses with certain phenotypic characteristics of the lines studied, genes for discrete traits could be assigned to specific V chromosomes or V chromosome arms. From the frequency of V chromosomes that were involved in chromatin exchanges with or substituted for one of their homeologous counterparts in the A, B, and D wheat genomes, it was inferred that D. villosum belongs to the same phyletic lineage as T. urartu (donor of the A genome of wheat) and Aegilops speltoides (B genome), and that Ae. squarrosa (D genome) diverged earlier from D. villosum.     

Cytogenetics of wild and captive bred non-domestic animals

Cytogenetics of wild and captive bred non-domestic animals provides us with valuable information that can be implemented in wildlife management and species conservation strategies. In this review, we summarized the data published to date describing a range of chromosome abnormalities observed in non-domestic animals and their effect on phenotype. Two important factors that can potentially have drastic effects on captive breeding programs are discussed: presence of classic chromosome abnormalities, spontaneously-occurring and inherited, and intraspecific variations in chromosome number. Short-term consequences, primarily reduced reproductive efficiency, and long-term consequences, such as changes in population dynamics, are examined.     

Multi-locus analysis reveals a different pattern of genetic diversity for mitochondrial and nuclear DNA between wild and domestic pigs in East Asia

Background

A major reduction of genetic diversity in mtDNA occurred during the domestication of East Asian pigs. However, the extent to which genetic diversity has been lost in the nuclear genome is uncertain. To reveal levels and patterns of nucleotide diversity and to elucidate the genetic relationships and demographic history of domestic pigs and their ancestors, wild boars, we investigated 14 nuclear markers (including 8 functional genes, 2 pseudogenes and 4 intergenic regions) from 11 different chromosomes in East Asia-wide samples and pooled them with previously obtained mtDNA data for a combined analysis.

Principal Findings

The results indicated that domestic pigs and wild boars possess comparable levels of nucleotide diversity across the nuclear genome, which is inconsistent with patterns that have been found in mitochondrial genome.

Conclusions

This incongruence between the mtDNA and nuclear genomes is suggestive of a large-scale backcross between male wild boars and female domestic pigs in East Asia. Our data reveal the impacts of founder effects and backcross on the pig genome and help us better understand the complex demographic histories of East Asian pigs, which will be useful for future work on artificial selection.     

Dynamics of parasite communities and interactions between wild and domestic ruminants

Dynamics of parasite communities and host-parasite-environment interactions can be influenced by different factors. The present note, by discussing some field experiences in wild and domestic ruminant populations, approaches eco-epidemiology of abomasal nematodes in relation with host health and dynamics. Factors possibly playing a role in the host-parasite relationship are discussed, as well as possible use of macroparasites as ecological and sanitary indicators. The above topics are approached in a management perspective, in particular regarding interactions between domestic and wild ruminant populations.     

Limb morphology of domestic and wild canids: the influence of development on morphologic change

Biomechanical hypotheses are often invoked to explain the characteristic scaling of limb proportions. Patterns of static allometry and morphologic diversity, however, may also reflect the developmental mechanisms underlying morphologic change. In this study I document the importance of such developmental influences on the evolution of limb morphology in the extremely polymorphic domestic dog and in wild canid species. I use bivariate and discriminant function analyses to compare the limb morphology of adult dogs and wild canid species. I then compare ontogenetic allometry of four dog breeds with static allometry of domestic and wild canids. Results reveal, first, that there is considerable similarity between dogs and wild canid species; many wolf-like canids cannot be distinguished from domestic dogs of equivalent size. However, all dogs are consistently separated from fox-sized, wild canids by subtle but evolutionarily significant differences in olecranon, metapodial, and scapula morphology. Second, in domestic dogs the pattern of static allometry is nearly identical to that of ontogenetic allometry. This finding can be attributed to simple heterochronic alterations of postnatal growth rates. Apparently the diversity of limb proportions among adult domestic dogs and the observed difference between dogs and wild canids are somewhat predetermined, as they directly reflect the diversity of limb proportions evident during development of the domestic dog.     

Prevalence of Toxoplasma gondii antibodies in sera of domestic pigs and some wild game species from Zimbabwe

Serum samples of domestic pigs (Sus scrofa), elands (Taurotragus oryx), sable antelopes (Hippotragus niger), warthogs (Phacochoerus aethiopicus), bushpigs (Koiropotamus [Potamochoerus] koiropotamus), white rhinos (Ceratotherium simus), African buffalos (Syncerus caffer), wildebeest (Connochaetas taurinus), and African elephants (Loxodonta africana) from Zimbabwe were tested for Toxoplasma gondii IgG antibodies by the modified agglutination test (MAT) with whole formalized tachyzoites and mercaptoethanol. Sera were diluted at 1:25, 1:50, and 1:500 for MAT testing. Sera with antibodies in a 1:25 dilution were considered to have T. gondii infection. Toxoplasma gondii antibodies were found in 9.3% of 97 domestic pigs, 36.8% of 19 elands, 11.9% of 67 sables, 0 of 3 warthogs, 0 of 3 bushpigs, 50% of 2 white rhinos, 5.6% of 18 buffalos, 14.5% of 69 wildebeest, and 10.5% of 19 elephants examined.     

Reproductive behaviour in survival: a comparison between wild and domestic sheep

The recorded behaviour of wild species of Ovis at the beginning of the breeding season supports the proposal that mating is synchronized by the 'ram effect', a phenomenon already described in domestic sheep. Animals separate into exclusive male flocks, and flocks of females and young animals for most of the year. They reunite just before the rutting season. At lambing there appear to be behavioural mechanisms that ensure that ewes lamb in close proximity to one another. It is hypothesized that these behavioural characteristics of wild sheep help protect the newborn and that much of the reproductive and maternal behaviour of domestic sheep may be traced to comparable behaviour in wild species.     

Analysis of ribosomal RNA genes in somatic hybrids between wild and cultivated Solanum species

Ribosomal RNA genes were exploited as markers to identify somatic hybrids between Solanum tuberosum cv. Brodick and wild diploid Solanum species, S. megistacrolobum, S. sanctae-rosae and S. sparsipilum and DNA methylation as a possible regulatory factor in gene expression was investigated. Specific restriction enzyme/probe combinations revealed useful polymorphisms in the conserved coding and variable intergenic spacer regions of the ribosomal RNA genes. Some intermediate ribosomal RNA gene profiles indicate hybridity whereas others were characteristic of S. tuberosum cv. Brodick. This evidence is suggestive of somatic exchange/re-arrangement between the NOR region of S. sanctae-rosae and S. tuberosum cv. Brodick. Ribosomal RNA gene copy number analysis of the somatic hybrids did not reveal hexaploid values suggesting that these products are not symmetric hybrids derived from the parental diploid and tetraploid plants. The results indicate site-specific methylation of ribosomal RNA gene sequences for the parental plants; while some somatic hybrids display a reduction, others show an increase. The significance of the findings for somatic cell genetics and plant breeding studies is discussed.     

Genetic identification of wild and domestic cats (Felis silvestris) and their hybrids using Bayesian clustering methods

Crossbreeding with free-ranging domestic cats is supposed to threaten the genetic integrity of wildcat populations in Europe, although the diagnostic markers to identify "pure" or "admixed" wildcats have never been clearly defined. Here we use mitochondrial (mt) DNA sequences and allelic variation at 12 microsatellite loci to genotype 128 wild and domestic cats sampled in Italy which were preclassified into three separate groups: European wildcats (Felis silvestris silvestris), Sardinian wildcats (Felis silvestris libyca), and domestic cats (Felis silvestris catus), according to their coat color patterns, collection localities, and other phenotypical traits, independently of any genetic information. For comparison, we included some captive-reared hybrids of European wild and domestic cats. Genetic variability was significantly partitioned among the three groups (mtDNA estimate of F(ST) = 0.36; microsatellite estimate of R(ST) = 0.30; P < 0.001), suggesting that morphological diversity reflects the existence of distinct gene pools. Multivariate ordination of individual genotypes and clustering of interindividual genetic distances also showed evidence of distinct cat groups, partially congruent with the morphological classification. Cluster analysis, however, did not enable hybrid cats to be identified from genetic information alone, nor were all individuals assigned to their populations. In contrast, a Bayesian admixture analysis simultaneously assigned the European wildcats, the Sardinian wildcats, and the domestic cats to different clusters, independent of any prior information, and pointed out the admixed gene composition of the hybrids, which were assigned to more than one cluster. Only one putative Sardinian wildcat was assigned to the domestic cat cluster, and one presumed European wildcat showed mixed (hybrid) ancestry in the domestic cat gene pool. Mitochondrial DNA sequences indicated that three additional presumed European wildcats might have hybrid ancestry. These four cats were sampled from the same area in the northernmost edge of the European wildcat distribution in the Italian Apennines. Admixture analyses suggest that wild and domestic cats in Italy are distinct, reproductively isolated gene pools and that introgression of domestic alleles into the wild-living population is very limited and geographically localized.