生物医学光子学中俄双边学术交流会

时间:2006年9月2日~3日地点:中国·武汉会议主席:Valery V.Tuchin(国立萨拉托夫大学[俄])骆清铭(华中科技大学)已确认的部分邀请报告人名单(报告题目详见会议网站:http://www.hust.edu.cn/pibm2006)俄方报告人:Victor N.Bagratashvili Anna A.Gavrilova Valentin M.GelikonovMikhail Yu.Kirillin Alexey Myakov Alexander B.PravdinAlexander V.Priezzhev Alexander P.Savitsky Ekaterina A.SergeevaValery V.Tuchin中方报告人(按姓氏笔划为序):马辉(清华大学)孔祥贵(中国科学院长春光机所)龙勉(中国科学院力学研究所)邢达(华…     

新书推荐:<生物医学光子学进展>

生物医学光子学是近年来受到国际生物医学界和光子学界关注的一个热点。随着最近几年的发展 ,该学科已包括了光子学、电子学、计算机、生命科学、数学、物理等学科的交叉与融合。生物医学光子学的研究必将对人类生命科学的发展产生重大影响。本书是一部重要的学术研究参考书 ,书中收集了最近几年在美国、加拿大、澳大利亚、日本等国家华人学者的学术论文。例如 :数字射线照相术、组织光学成像技术与图像重建、大脑活动与功能的近红外光学成像、双光子成像技术在神经科学中的应用、基于绿色荧光蛋白的显微活体成像等都是目前该领域的最新…     

生物发光成像无损伤研究植物生物钟的方法

植物生物钟系统是植物为了适应地球自转进化出的以约24小时为周期的分子系统,通过感知并整合外界周期性变化的环境信号进而协调细胞内相应基因的表达和能量状态,赋予植物对生存环境的适应性并参与调控多个植物生长发育过程。目前,越来越多的研究聚焦于解析植物生物钟的分子机制,基于此也衍生出很多研究生物钟表型的方法。该文在总结已有生物钟检测方法的基础上,重点介绍生物钟表型实验中最常用且比较稳定可靠的实验方法,以期为生物钟的表型研究尤其是生物钟机制研究提供技术支持与借鉴。     

生物发光成像无损伤研究植物生物钟的方法

植物生物钟系统是植物为了适应地球自转进化出的以约24小时为周期的分子系统, 通过感知并整合外界周期性变化的环境信号进而协调细胞内相应基因的表达和能量状态, 赋予植物对生存环境的适应性并参与调控多个植物生长发育过程。目前, 越来越多的研究聚焦于解析植物生物钟的分子机制, 基于此也衍生出很多研究生物钟表型的方法。该文在总结已有生物钟检测方法的基础上, 重点介绍生物钟表型实验中最常用且比较稳定可靠的实验方法, 以期为生物钟的表型研究尤其是生物钟机制研究提供技术支持与借鉴。     

第五届生物医学光子学与成像技术国际学术研讨会第二轮通知

举办时间:会议2006年9月1日~3日,展览2006年9月2日~3日主办单位:武汉光电国家实验室(筹),生物医学光子学教育部重点实验室,国际光学工程学会俄罗斯分会会展地点:中国·武汉会议简介第五届生物医学光子学与成像技术国际学术研讨会将于2006年9月1日~3日在武汉华中科技大学举行。本次会议的内容涵盖从基础研究到仪器研发、从生物学研究到临床应用等方面,将吸引来自世界各地生物医学光学和成像技术领域的杰出学者参加。与会议同期举办的展览会将为学术界、产业界和政府机构的与会者提供展示与交流的平台,寻求技术上的创新与突破。本次会议包括一…     

《生物医学光子学前沿及“十五”发展战略研讨会》在福州召开

由国家自然科学基金委主办、福建师范大学激光研究所承办的《生物医学光子学前沿及“十五”发展战略研讨会》于 2 0 0 2年 5月 1 3日至 1 6日在福建省福州市福建师范大学召开。国家自然科学基金委信息科学部原主任王玉堂、信息科学部四处处长何杰以及我国生物医学光子学领域的部分专家代表 32人参加了本次会议。福建省科技厅厅长、福建师范大学校长等出席了开幕式 ,并盛情设宴款待了会议代表。本次会议旨在通过学术交流和座谈等多种形式 ,调研了国内外生物医学光子学前沿发展动态 ,归纳总结了生物医学光子学领域带有战略性的关键问题 ,提出…     

分子生物光子学的进展

随着分子生物学和标记技术的进步,光学成像和分析有了很快的发展,无论是从理论上还是应用上都有所突破,展现了良好的前景。本文综述了近年来在分子生物探测中光子学的情况,简介了几种突破光学衍射极限的探测方法,着重阐明了与生化技术密切相关的荧光共振能量转移和荧光相关光谱显微术及其在分子生物学中的应用。     

生物光子学应用

生物光子学是光学技术与生命科学的交叉学科,可以在分子水平上研究细胞的功能和结构,包括生物系统的光子辐射以及这些光子携带的信息,用光子及其技术对生物系统进行检测、加工和改造等。激光扫描共焦显微术、双光子荧光显微术、近场光学扫描显微术和光镊等显微技术在生命科学研究中的应用非常重要。     

中医经络的光子学研究

在中医经络现代研究的基础上,探讨光子学在经脉循行路线的客观检测,循经感传现象的发生及其实质意义,经络腧穴诊断的客观化以及腧穴双向良性调节作用等研究领域的具体应用.指出中医经络的光子学研究有望借助光子学的学科优势,从科学的角度对我国经络学说进行诠释和量化,为经脉腧穴的光学特性提供重要的实验依据,并有望找到一种非侵人性、无...     

生物医学光学中NADH荧光检测技术的发展

NADH(还原型烟酰胺腺嘌呤二核苷酸)自1962年被发现至今已有五十多年了,在此期间,运用NADH及其相关技术进行医疗诊断与医学检测的方式不断发展,领域也不断扩大。从当前的研究成果来看,NADH因为其独有的性质在现代医学诊断与检测领域发挥了极大的作用,如实时、无损地监测脑部的活动;清晰、有效地识别不同种类的脑组织;抗细胞辐射损伤;抗辐射诱导的细胞凋亡;进行人体内的诸多平衡调节及人体生物钟调节等。本文将重点介绍在重要领域中的NADH研究和应用的突破。     

多光子发光的稀土上转换纳米颗粒在生物光子学中的研究进展

生物医学光子学的发展,总是伴随并促进着光子学新技术的发展。光学生物成像技术在癌症肿瘤诊断上有着巨大应用,尤其是具有优良发光特性的稀土离子掺杂的上转换发光纳米颗粒与光学生物成像技术的结合进一步发展了生物光子学在这一领域的应用。鉴于近几年很多人对上转换发光纳米粒子的大量研究,本文对其进行了系统的阐述,综述了稀土上转换发光纳米粒子的光学特异性、发光原理及其在光学成像中不可替代的优势;描述了上转换纳米粒子的化学组成,介绍了几种基本的合成方法,重点说明了水热合成法和热分解法,并从材料和光学两方面分析了生物应用的效率优化;总结了目前上转换材料在生物光子学中的几大应用,着重介绍了生物传感、细胞成像、动物成像、漫射光层析成像、光动力治疗、多模式成像六个方面的应用。本文在最后也对今后的研究进行了展望。     

Noninvasive bioluminescence imaging in small animals

There has been a rapid growth of bioluminescence imaging applications in small animal models in recent years, propelled by the availability of instruments, analysis software, reagents, and creative approaches to apply the technology in molecular imaging. Advantages include the sensitivity of the technique as well as its efficiency, relatively low cost, and versatility. Bioluminescence imaging is accomplished by sensitive detection of light emitted following chemical reaction of the luciferase enzyme with its substrate. Most imaging systems provide 2-dimensional (2D) information in rodents, showing the locations and intensity of light emitted from the animal in pseudo-color scaling. A 3-dimensional (3D) capability for bioluminescence imaging is now available, but is more expensive and less efficient; other disadvantages include the requirement for genetically encoded luciferase, the injection of the substrate to enable light emission, and the dependence of light signal on tissue depth. All of these problems make it unlikely that the method will be extended to human studies. However, in small animal models, bioluminescence imaging is now routinely applied to serially detect the location and burden of xenografted tumors, or identify and measure the number of immune or stem cells after an adoptive transfer. Bioluminescence imaging also makes it possible to track the relative amounts and locations of bacteria, viruses, and other pathogens over time. Specialized applications of bioluminescence also follow tissue-specific luciferase expression in transgenic mice, and monitor biological processes such as signaling or protein interactions in real time. In summary, bioluminescence imaging has become an important component of biomedical research that will continue in the future.     

Biomedical applications of fluorescence imaging in vivo

Optical imaging can advance knowledge of cellular biology and disease at the molecular level in vitro and, more recently, in vivo. In vivo optical imaging has enabled real-time study to track cell movement, cell growth, and even some cell functions. Thus, it can be used in intact animals for disease detection, screening, diagnosis, drug development, and treatment evaluation. This review includes a brief introduction to fluorescence imaging, fluorescent probes, imaging devices, and in vivo applications in animal models. It also describes a quantitative fluorescence detection method with a reconstruction algorithm for determining the location of fluorophores in tissue and addresses future applications of in vivo fluorescence imaging.     

近红外无创伤血糖测量的组织光学基础研究

人体血糖浓度无创伤测量是当今学术界和医学界普遍关注的课题。在分析了血糖浓度无创伤测量的意义、现有的测量方法及其进展后,从组织光学角度分析血糖浓度无创伤测量中包含的研究内容,并根据当前的研究现状,提出血糖浓度无创伤测量所存在的问题。通过组织光学角度对血糖浓度无创伤测量方法的剖析,更加明确血糖测量的研究任务,有望促使其更进一步的发展以及血液中其他成分的无创测量。     

Noninvasive imaging of cardiac electrophysiology

Noninvasive imaging of cardiac electrophysiology is still a major goal despite all recent technical innovations. This review gives an overview about the historical background, recent developments and possible future applications of noninvasive imaging of cardiac electrophysiology.     

Noninvasive imaging of quantum dots in mice

Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for detection. Circulating half-lives were found to be less than 12 min for amphiphilic poly(acrylic acid), short-chain (750 Da) methoxy-PEG or long-chain (3400 Da) carboxy-PEG quantum dots, but approximately 70 min for long-chain (5000 Da) methoxy-PEG quantum dots. Surface coatings also determined the in vivo localization of the quantum dots. Long-term experiments demonstrated that these quantum dots remain fluorescent after at least four months in vivo.     

Noninvasive fluorescent imaging reliably estimates biomass in vivo

Whole-body optical imaging of small animals has emerged as a powerful, user friendly, and high-throughput tool for assaying molecular and cellular processes as they occur in vivo. As with any imaging method, the utility of such technology relies on its ability to provide quantitative, biologically meaningful information about the physiologic or pathologic process of interest. Here we used an animal tumor model to evaluate the extent of correlation between noninvasively measured fluorescence and more traditional measurements of biomass (tumor volume and tumor weight). C57/BL6 mice were injected subcutaneously with murine colon adenocarcinoma cells that were engineered to express GFP. Serial measurements of fluorescence intensities were performed with a macroscopic in vivo fluorescence system. The progressive increases in intensity correlated strongly with growth in tumor volume, as determined by caliper measurements (R2 = 0.99). A more stringent correlation was found between fluorescence intensity and tumor weight (R2 = 0.97) than between volume and weight (R2 = 0.89). In a treatment experiment using tumor necrosis factor-alpha, fluorescence intensity (but not tumor volume) was able to differentiate between treated and control groups on day 1 post-treatment. These results validate the ability of noninvasive fluorescent imaging to quantify the number of viable, fluorescent cells in vivo.