Two New Saponins from Lysimachia capillipes Hemsl.

To investigate the saponins from whole plants of Lysimachia capillipes Hemsl., two new saponins, named capilliposide E (1) and capilliposide F (2), were isolated. The structures of the new sa ponins were elucidated as 3 β, 22α-dihydroxy- 16α-acetat-28→ 13 -lactone-oleanane-3 -O- [β-D-glucopyranosyl(1→2)-α-L-arabinpyranoyl]-22-O-β-D-glucopyranoside (1) and 3 β, 22α-dihydroxy- 16α-acetat-28→ 13-1actone-oleanane-3-O- { [β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)]-α-L-arabinpyranoyl }-22-O-βD-glucopyranoside (2). The structures of these compounds were determined by 1D- and 2D-NMR, MS techniques, and chemical methods.     

Saponins from Lysimachia candida

Three saponins were isolated from Lysimachia candida Lindl. By spectral and chemical analysis, they were identified as primulagenin A-3-O-β-D-xylopyranosy( 1→2)-β-D-glucopyranosyl( 1→4)-[β-D-glu- copyranosyl(1→2) ] -a-L-arabinopyranoside ( 1 ), protoprimulagenin A-3-O-β-D-xylopyranosyl( 1→2)-β-D-glu- copyranosyl( 1→4)-[ β-D-glucopyranosyl ( 1 →2) ]-a-L-arabinopyranoside ( lysikoianoside, 2) and a-spinas- teml-glucopyranoside (3) respectively. 1 is a new compound named candidoside.     

巴东过路黄中三萜皂苷及其体外抗肿瘤活性研究

从巴东过路黄(Lysimachia patungensis)95%乙醇提取物的正丁醇萃取部位中,分离到2个齐墩果烷型三萜皂苷,经光谱鉴定,分别为ardicrenin(1)和ardisiacrispinA(2)。体外抗肿瘤实验显示ardicrenin(1)对人脑胶质瘤(SWO-38)、口腔上皮癌(KB)、人乳腺癌(MCF-7)和人宫颈癌(Hela)细胞的半数毒性浓度(TC50)分别为3.16、3.16、2.97、2.42μmol/L,ArdisiacrispinA(2)对上述细胞的TC50分别为3.96、3.01、1.98、2.73μmol/L。     

珍珠菜属植物三帖类化合物研究进展

对珍珠菜属(Lysimachia)植物中已报道的三萜类化合物化学结构和生物活性研究进行了综述。本属中的三萜皂苷均以齐墩果烷型五环三萜为苷元基本骨架,根据皂苷元结构,可将皂苷分为13β,28-环氧-齐墩果烷型(Ⅰ型)和12-烯-齐墩果烷型(Ⅱ型),同时论述了Ⅰ型和Ⅱ型皂苷元化学结构转换。     

五种珍珠菜的核型研究

本文对５种珍珠菜亚属植物进行了染色体数目及核型研究。其中瓣珍珠菜２ｎ＝２４＝１２ｍ＋８ｓｍ＋４ｓｔ（２ＳＡＴ）、黑腺珍珠菜２ｎ＝２２＝２ｍ＋４ｓｍ＋６ｓｔ＋１０ｔ、泽珍珠菜２ｎ＝２４＝１４ｍ＋６ｓｍ（２ＳＡＴ）＋４ｓｔ和小叶珍珠菜２ｎ＝４８＝３４ｍ＋１０ｓｍ＋４ｓｔ的染色体数目及核型为首次报道。中国九江产的红根草核型２ｎ＝２４＝２０ｍ＋４ｓｍ（２ＳＡＴ）与日本产的核型２ｎ＝２４＝１８ｍ（１ＳＡＴ）     

多荚草中的新三萜皂甙

从石竹科植物多荚草（Ｐｏｌｙｃａｒｐｏｎ ｐｒｏｓｔｒａｔｕｍ（Ｆｏｒｓｓｋ．）Ａｓｃｈｅｒｓ．ｅｔ Ｓｃｈｗｅｉｎ．ｅｘ Ａｓｃｈｅｒｓ）中分离得到３个新的柴胡皂甙类化合物：ｐｒｏｓｔｒａｔｏｓｉｄｅ Ａ￣Ｃ（１￣３）。它们的结构通过波谱方法分别鉴定为：３－Ｏ－｛β－Ｄ－ｘｙｌｏｐｙｒａｎｏｓｙｌ－（１→２）－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ－（１→４）－「β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙ     

Two New Triterpenoid Saponins from Akebia quinata （Thunb.） Decne.

Two new triterpenoid saponins, hederagenin 3-O-α-L-arabinopyranosyl-（1→〉2）-α-L-arabinopyranoside named akeboside La （compound 1）, oleanolic acid 3-O-α-L-arabinopyranosyl-（1→〉2）-β-D-glucopyranoside named akeboside Lb （compound 2）, along with five known saponins, oleanolic acid 3-O-α-L-rhamnopyranosyl-（1→〉2）-α-L- arabinopyranoside （compound 3）, hederagenin 3-O-α-L-rhamnopyranosyl-（1→〉2）-α-L-arabinopyranoside （compound 4）, oleanolic acid 3-O-β-D-xylopyranosyl-（1→〉3）-α-L-rhamnopyranosyl-（1→〉2）-α-L-arabinopyranoside （compound 5）, 3-O-α-L-rhamnopyranosyl-（1→〉2）-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl-（1→〉4）-α-D- glucopyranosyl-（1→〉6）-β-D-glucopyranoside （compound 6）, 3-O-α-L-rhamnopyranosyl-（1→〉2）-α-L-arabinopyranosyl hederagenin 28-α-L-rhamnopyranosyl-（1→〉4）-β-D-glucopyranosyl-（1→〉6）-β-D-glucopyranoside （compound 7） were isolated from the n-butanol part of the 80% ethanol extracts of the dried stems of Akebia quinata （Thunb.） Decne. Compound 5 was isolated from plants of genus Akebia for the first time. The structures were elucidated on the basis of physicochemical properties and spectral data.     

New Cytotoxic Triterpenoid Saponins from the Roots of Albizia gummifera (J.F.Gmel.) C.A.Sm.

As part of our search for new bioactive saponins from Cameroonian medicinal plants, two new oleanane‐type saponins, named gummiferaosides D and E ( 1 and 2 ), along with one known saponin, julibroside J₈ ( 3 ), were isolated from the roots of Albizia gummifera. Their structures were established on the basis of extensive 1D‐ and 2D‐NMR (¹H‐ and ¹³C‐NMR, DEPT, COSY, TOCSY, NOESY, HSQC, HSQC‐TOCSY, and HMBC) and HR‐ESI‐MS studies, and by chemical evidence. The apoptotic effect of saponins 1  –  3 was evaluated on the A431 human epidermoid cancer cell. Flow cytometric analyses showed that saponins 1  –  3 induced apoptosis of human epidermoid cancer cell (A431) in a dose‐dependent manner.     

基于ITS序列探讨珍珠菜属过路黄组的系统关系

测定了紫脉过路黄、临时救和过路黄的nrDNA ITS序列,并分析了珍珠菜属过路黄组17个物种的遗传距离及亲缘关系。结果表明,过路黄组植物的ITS序列长度在620～628间,一致性高达90.59%,种间遗传距离为0.002～0.199。系统发育树表明:(1)紫脉过路黄、临时救和小茄亲缘关系较近;(2)大叶过路黄、落地梅和过路黄亲缘关系较近;(3)山萝过路黄、贯叶过路黄、管茎过路黄、叶头过路黄、峨眉过路黄及三角叶过路黄亲缘关系较近。ITS序列分析结果为组内植物的鉴定、分类及系统进化提供了新的参考。     

白花刺参中两个新三萜皂甙

从著名藏药白花刺参(Morina nepalensis var.alba Hand.-Mazz.)的水溶性部分分离到2个新三萜皂甙--刺参甙K(1)和刺参甙L(2),以及一个已知三萜皂甙mazusaponin I(3).应用波谱和化学方法,刺参甙K和刺参甙L的结构分别鉴定为3-O-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl siaresinolic acid(1)和3-O-β-D-glucopyranosyl-(1→3)-α-L-arabinopyranosyl siaresinolic acid 28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(2).     

无患子中两个新的大戟烷型三萜皂甙

从无患子科无患子（Sapindus mukorossi Gaertn.)根中分离到两个新的大戟烷型三萜皂甙SapimukosideA（1）和SapimukosideB（2）。运用波谱和化学方法鉴定它们的结构为3－O－α-L鼠李吡喃糖基－（1→2）-[α-L－阿拉伯吡喃糖基－（1→3）]－β－D－葡萄吡喃糖基－21,23R－epoxyl tirucall-7,24R-diene-3β,21-diol(1)和3－O－α-L－鼠李吡喃糖基－（1→6）－β-D-葡萄吡喃糖基21,23R-epoxyl tirucall-7,24R-diene-3β,21-diol(2)。     

海星中的皂甙和多羟基甾体化合物

海洋无脊椎动物海星的次级代谢产物以皂甙和多羟基甾醇为主,且多具有明显的生理活性。本文综述了近十年来海星中皂甙和多羟基甾体化合物的研究进展。     

Triterpenoid Saponins with Potential Cytotoxic Activities from the Root Bark of Ilex rotunda Thunb.

A new 19‐oxo‐18,19‐seco‐ursane‐type triterpeonoid saponin, laevigin E ( 8 ), together with 17 known compounds ( 1 – 7 and 9 – 18 ) were isolated from the root bark of Ilex rotunda Thunb . Their structures were determined by various spectroscopic analysis. Among them, compounds 6 , 9 , 11 , and 18 were isolated from this species for the first time, while compounds 10 and 12 were firstly isolated from the family Aquifoliaceae. Biological activity assay showed that all triterpenoids exhibit moderate cytotoxic activities against MCF7, A549, HeLa and LN229 cell lines. The four triterpenoid saponins ( 3 , 4 , 6 , and 8 ) exhibit slightly better activities compared to the four triterpenoid sapogenins ( 1 , 2 , 5 , and 7 ). Compound 8 showed the best cytotoxicity against A549, HeLa and LN229 cell lines with IC₅₀ of 17.83, 22.58 and 30.98 μm , respectively.     

Triterpenoid Saponins from Pulsatilla cernua (Thunb.)Bercht. et Opiz.

To investigate saponins from the roots of Pulsatilla cernua (Thunb.) Bercht. et Opiz., two new compounds together with five known triterpenoid saponins were isolated. The structures of the two new triterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl(1→2)]-[α-L-rhamnopyranosyl(1→4)]-α-Larabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 1) and 3-O-[α-L-arabinopyranosyl(1→3)]-[α-L-rhamnopyranosyl (1→2)]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester (compound 2) by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods.     

Triterpenoid Saponins from Pulsatilla cernua （Thunb.） Bercht. et Opiz.

To investigate saponins from the roots of Pulsatilla cernua （Thunb.） Bercht. et Opiz., two new compounds together with five known trlterpenold saponins were isolated. The structures of the two new trlterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl（1-）2）]-[α-L-rhamnopyranosyl（1-）4）]-α-L- arablnopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 1） and 3-O-[α-L-arabinopyranosyl（1→）3）]- [α-L-rhamnopyranosyl （1→）2）]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 2） by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods.     

Five New Oleanane Triterpene Saponins from Camellia petelotii and Their Alpha-glucosidase Inhibitory Activity

Five new triterpenoid glycosides, named campetelosides A–E ( 1–5 ), together with three known compounds, chikusetsusaponin IVa ( 6 ), umbellatoside B ( 7 ), and silvioside E ( 8 ) were isolated from the leaves of Camellia petelotii (Merr.) Sealy. Their chemical structures were determined by interpretations of HR-ESI-MS and NMR spectra. In addition, compounds 1–8 were evaluated for their α-glucosidase inhibitory effects. Compounds 1–3 significantly showed α-glucosidase inhibitory activity with IC₅₀ values of 166.7±6.0, 45.9±2.6, and 395.3±10.5 μM, respectively, compared to that of the positive control, acarbose, with an IC₅₀ value of 200.4±10.5 μM.     

无患子中两个新的大戟烷型三萜皂甙

从无患子科无患子(Sapindus mukorossi Gaertn.)根中分离到两个新的大戟烷型三萜皂甙Sapimukoside A (1) 和Sapimukoside B (2).运用波谱和化学方法鉴定它们的结构为3-O-α-L-鼠李吡喃糖基-(1→2)-[α-L-阿拉伯吡喃糖基-(1→3)]-β-D-葡萄吡喃糖基-21, 23R-epoxyl tirucall-7, 24R-diene-3β, 21-diol (1) 和3-O-α-L-鼠李吡喃糖基-(1→6)-β-D-葡萄吡喃糖基21, 23R-epoxyl tirucall-7, 24R-diene-3β, 21-diol (2).     

白花刺参中两个新三萜皂甙

从著名藏药白花刺参（Morina nepalensis var.alba Hand.-Mazz)的水溶性部分分离到2个新三萜皂甙-刺参甙K（1）和刺参甙L（2）,以及一个已知三萜皂甙mazusaponinⅠ（3)。应用波谱和化学方法,刺参甙K和刺参甙L的结构分别鉴定为3-O-α-L-arabinopyranosyl-（1→）-β-D-xylopyranosyl siaresinolic acid(1)和3-O-β-D-glucopyranosyl-(1→3)-α-L-arabinopyranosyl siaresinolic acid28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(2)。