首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到18条相似文献,搜索用时 562 毫秒
1.
中国林蛙乳酸脱氢酶多基因系统及基因间连锁关系的研究   总被引:4,自引:0,他引:4  
张辉  吴清江 《遗传学报》1996,23(1):11-17
(1)用聚丙烯酰胺凝胶电泳对山西产中国林蛙4个地理群的333只林蛙进行了分析,结果表明:中国林蛙的LDH由LDH-A,LDH-B和LDH-C3个基因决定。LDH-A是单态座位,LDH-B和LDH-C均为多态座位,每个多态座位均有两个等位基因。LDH-B与LDH-C呈紧密连锁关系。认为LDH-C是LDH-B的重复产物。(2)热稳定性、尿素处理稳定性及组织特异性研究表明:LDH对温度和尿素处理稳定性顺序为A4>B’4>B4>C4。A4在骨骼肌和肝脏等组织中活力最大,B4在心肌和卵巢中活力最强,LDH-C主要在眼球和卵巢中表达。(3)Ldh-b和Ldh-b'在不同地理群间呈差异分布,随着纬度的增高,Ldh-b在种群中的频率增大。  相似文献   

2.
金晓玲  何新霞 《兽类学报》1998,18(2):144-149
用电泳比较分析了鼠科动物6个种的组织LDH同工酶的A、B、C亚基和SOD同工酶位点的种属差异。结果表明,LDH的A和B亚基在进化上的可变性程度不同。在被分析的2个属共6种鼠中,首次发现B亚基的结构具有属的特征,同属的鼠种B亚基泳动度相同,在垂直凝胶电泳板上处于同一泳动线上。不同属的鼠其B亚基泳动度不同,家鼠属的鼠B亚基比姬鼠属的泳动快;A亚基的结构既有属的特性,更具有种的特征,同属不同种的鼠A亚基泳动速度不同,表现为LDH同工酶带的间距不同。LDH的C亚基结构因鼠种不同而异,由C亚基组成的LDH-X带黄毛鼠位于自身的LDH-3和LDH-4之间,社鼠和褐家鼠此带位于自身的LDH-4和LDH-5之间,白腹巨鼠此带位于自身的LDH-5以外的阴极端,与黑线姬鼠此带的位置特征相同。SOD同工酶带只表现种的差异,其3条带泳动速度的改变具有协同而变的共同特点。被分析的鼠种经配对法比较生化特征的相同程度,初步表明黄毛鼠亲缘上与褐家鼠比较近,白腹巨鼠亲缘上又比其余3种鼠更近于姬鼠属。  相似文献   

3.
作者采用三种垂直板聚丙烯酰胺凝胶电泳对雌雄黄鳝6种组织的乳酸脱氢酶(LDH)、酯酶(EST)进行了研究。结果表明LDH同工酶谱为细带型,可分为3区8条带,其迁移率为A>B>C,其中C_4仅出现在心、脑和肌肉中。LDH在肝中表达甚微。EST同工酶显示3区7条带。黄鳝的同工酶有明显的组织特异性,在两性中的表达也存在一定的差异,这种差异EST比LDH明显;肝、肾和性腺比其它组织较明显。这两种同工酶在雌性中的表达均比在雄性中强。以上反映了差别的基因活性。本文还讨论了黄鳝同工酶的遗传基础,亚基的组成以及LDH迁移率和黄鳝演化地位的关系。  相似文献   

4.
徐晋麟  马飞 《遗传学报》1994,21(2):104-111
作者采用三种垂直板聚丙烯酰胺凝胶电泳对雌雄黄鳝6种组织的乳酸脱氢酶(LDH)、酯酶(EST)进行了研究。结果表明LDH同工酶谱为细带型,可分为3区8条带,其迁移率率为A>B>C,其中C4仅出现在心、脑和肌肉中。LDH在肝中表达甚微。EST同工酶显示3区7条带。黄鳝的同工酶有明显的组织较明显。在两性中的表达也存在一定的差异,这种差异EST比LDH明显;肝、肾和性腺比其它组织较明显。这两种同工酶在雌性  相似文献   

5.
幼年和成年小白鼠LDH与SOD动态变化的电泳分析   总被引:2,自引:0,他引:2  
采用聚丙烯酰胺凝胶圆盘电泳分析方法,研究了幼年鼠和成年鼠睾丸乳酸脱氢酶(LDH)同工酶与超氧化物歧化酶(SOD)的酶带变化。观察结果表明,成年鼠睾丸的凝胶电泳胶条,多数显现六种LDH同功酶带,其中LDH5和LDH4占优势,LDH1、LDH2、LDH3显带较弱,在正极端LDH的下面是第6条酶带,相当于C亚单位(LDHc)的C4。幼年鼠睾丸的凝胶电泳胶条,只显现LDH1和LDH2两条同功酶带,以LDH  相似文献   

6.
用FOX(Ferrousionoxidationinpresenceofferricionindicatorxylenolorangeformeasurementofhydroperoxides)法直接测定脂氢过氧化物(LOOH),观察了低密度脂蛋白(LDL)氧化修饰过程中LOOH变化的动力学,研究了抗氧化剂probucol、BHT、VE、VC和β-胡萝卜素对LDL氧化修饰的抑制作用,并比较了它们抑制能力的大小。结果显示:LDL氧化修饰过程中LOOH的变化呈现三个时相:第一为延迟期,LOOH几乎没有变化;第二为扩增期,LOOH急剧增加;第三为降解期,LOOH达到最大值并开始出现下降趋势。抗氧化剂可以使延迟期的时间延长,延长的时间长短与抗氧化剂的浓度成正比。以VE或VC为标准,求出不同浓度各种抗氧化剂延长延迟期的时间及其相应的VE或VC浓度,计算出其浓度与相对的VE或VC间的倍数,通过比较倍数的大小,得出其抑制LDL氧化修饰能力的大小顺序为:BHT>Probucol>VE>VC>β-胡萝卜素。  相似文献   

7.
HDL受体和肝性脂酶在肝选择性摄取HDL_2-CE中的协同作用   总被引:2,自引:0,他引:2  
体外重组3H-CE-无ApoE-HDL2(rHDL2)保持天然HDL2生物活性。大鼠肝窦状隙细胞与rHDL237℃培养3h(正常组);细胞内吞cpm为995±147(x±S.D.,n=2)。细胞进一步97℃培养2h.释放三氯醋酸(TCA)沉淀和TCA上清液中cpm为78±32和12±9。N-乙酰咪唑修饰组为339±62、19±11和9±5。肝素处理组为542±78、34±14和9±8。实验结果提示:(1)肝窦状隙细胞通过HDL受体内吞HDL2摄取HDL2-CE,并通过逆向胞饮将HDL3排出体外.(2)肝性脂酶(HL)直接介导细胞选择性摄取HDL2-CE,导致HDL3生成.(3)HL和HDL受体在介导细胞选择性摄取HDL2-CE中具有协同作用。  相似文献   

8.
本文采用酶细胞化学方法,在光镜下用积分分级计数法,全自动图像分析仪检测精子LDH-C4活性,两法测定LDH-C4活性显著正相关(r=0.801和0.742,P<0.01);LDH-C4酶细胞化学电镜技术观察精子LDH-C4活性,通过计数酶点可测定LDH-C4活性。本文检查38例不育男性和20例已生育男性精子LDH-C4活性,LDH-C4主要分布于精子STM及胞质,其次是顶体及质膜表面。可通过胞膜外逸到精浆,LDH-C4在各部活性比值为5∶2∶1∶1。不育组精子LDH-C4可能通过膜破损处外漏到精浆使精子内LDH-C4显著减少,LDH-C4在精子顶体亦显著减少,使不育组精子LDH-C4活性显著低于生育组精子,提示LDH-C4活性的定位和大小直接与生育能力相关,检测LDH-C4活性可作为检查不育症精子质量的可靠指标。LDH-C4与精子密度显著相关(r=0.737和-0.493,P<0.05);与活动性无相关性。  相似文献   

9.
动脉平滑肌细胞(sm ooth m uscle cell,SMC)是动脉粥样硬化(atherosclerosis,AS)斑块中的主要细胞,它的增殖在AS形成过程中极其重要.利用体外培养的人主动脉SMC,观察了天然高密度脂蛋白(native high density lipoprotein,N-HDL)及氧化修饰HDL(oxidized HDL,OX-HDL)对培养人主动脉SMC cyclin D1(细胞周期蛋白D1)基因转录表达的影响.结果表明:(1)N-HDL对SMCcyclin D1基因表达无影响(P> 0.05);(2)OX-HDL使SMCcyclin D1基因表达显著增强(P<0.01),其表达量随时间(2、12、24 h)延长而增加.上述结果表明,OX-HDL的致AS作用可能与其刺激SMCcyclin D1基因表达增加有关.  相似文献   

10.
动脉平滑肌细胞(SMC)是动脉粥样硬化(AS)斑块中的主要细胞,它的增殖在AS形成过程中极其重要。脂蛋白和氧化修饰型脂蛋白对SMC增殖的影响以及SMC增殖与原癌基因异常表达的关系是当前AS发病机制研究的热点之一。我们在建立人主动脉SMC体外培养方法的基础上,观察了LDL,VLDL及HDL和相应的氧化修饰型脂蛋白对培养人SMCfos,myc,erb-B原癌基因转录表达的影响。结果表明:①HDL对SMCfos,myc基因表达无影响;②LDL和VLDL有使这些基因表达增加的趋势,但与对照比较差异不显著(P>0.05);③OX-VLDL,OX-VLDL和OX-HDL有使SMCfos,myc基因表达显著增强的作用(P<0.01),且其作用较相应的天然脂蛋白大(P<0.01).上述结果说明:LDL,VLDL,OX-LDL,OX-VLDL和0X-HDL的致AS作用可能与刺激SMCfos和myc癌基因表达增加有关。  相似文献   

11.
The ontogeny of the lactate dehydrogenase (LDH; EC 1.1.1.27) isozymes during medaka (Oryzias latipes) embryogenesis was determined after the genetic and molecular bases of this multilocus isozyme system were established. Three LDH loci are differentially expressed among the tissues of the adult medaka. The LDH-A locus was expressed almost exclusively in the white skeletal muscle, the LDH-B locus in all tissues examined, and the LDH-C locus in the eye and brain. The contribution of each of these LDH loci was quantitatively determined throughout early medaka embryogenesis by using a combination of electrophoretic, immunochemical, and spectrophotometric procedures. LDH-B4 is present throughout embryogenesis and is the predominant LDH isozyme during this period. LDH-C subunit activity was first detected 146 hr after fertilization (26°C), 142 hr prior to hatching. LDH-A subunit activity, however, was not detected until after hatching and, then, only as heterotetramers containing LDH-B subunits. The pattern of LDH gene expression during medaka embryogenesis was compared with the patterns of LDH gene expression during early development in five other teleost species. Some common patterns of differential LDH gene expression appear to exist among the teleosts. In all species examined, isozymes encoded in at least one LDH locus, A and/or B, were present throughout development. Those isozymes present continually during embryogenesis also tend to be active in a wide variety of differentiated tissues in the adult fish. Conversely, LDH isozymes which are active in a restricted number of adult tissues are detected only later in embryogenesis. The initiation of LDH-C gene expression, however, is closely coupled with morphological and functional differentiation of those cells in which this locus is predominantly expressed in the adult.  相似文献   

12.
采用垂直淀粉凝胶电泳及特异性组织化学染色技术,研究了草鱼成体脑、眼、心、肾、肌、肝等6种组织中的6种同工酶系统(LDH、MDH、GDH、ADH、LDH、EST)的分化表达谱式。结果表明,草鱼的同工酶系统具有明显的组织特异性。与绝大多数硬骨鱼类相比,草鱼的LDH、m-MDH和ADH同工酶具有特殊的表达谱式:m-MDH和ADH均由两个基因座位编码;肾脏在LDH-A_3B与LDH-A_2B_3之间多出1条LDH酶带(LDH-X)。本文还讨论了草鱼同工酶的遗传基础和亚基组成,以及本实验的某些结果与其他作者的结果不相符的原因。  相似文献   

13.
《FEBS letters》1993,330(3):307-311
Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, liver-type and kidney(non-hepatic)-type enzymes. The developmental expression of these two isozyme proteins has been investigated in rat liver using immunohistochemical techniques. The liver-type AdoMet synthetase is expressed only in adult liver, but not in fetal liver. On the other hand, the kidney-type AdoMet synthetase is predominantly expressed in fetal liver and faintly detected in adult liver. It was also found that both isozymes were localized to the hepatocytes of rat liver. These results clearly show that AdoMet synthetase isozymes are developmentally regulated within hepatocytes. In addition, in rat kidney we have shown that the kidney-type AdoMet synthetase is predominantly localized to the distal tubule.  相似文献   

14.
鲇鱼不同组织同工酶的组织特异性的初步研究   总被引:3,自引:0,他引:3  
采用聚丙烯酰胺垂直凝胶电泳法(PAGE)对鲇鱼的9种组织的5种同工酶(LDH、MDH、ADH、SUDH和EST)进行了分析,结果表明:5种同工酶在各种组织均有不同的表达程度:在心、肝、卵巢和肌肉中的表达频率最高,在脑和眼中最低。LDH和MDH在各个组织中均为强表达,与以往研究结果比较,认为鲇鱼LDH同工酶不存在C位点。鲇鱼同工酶组织特异性较明显。  相似文献   

15.
大鳞副泥鳅LDH同工酶的发育遗传学研究   总被引:4,自引:0,他引:4  
采用聚丙烯酸胶凝胶电泳技术研究了大群副泥鳅早则发育过程(受精后0一227小时)和成体7种组织器官(脑、肾、骨骼肌、肠、心、肝和服)中的LDH同工两表达状况.结果表明,大群副泥鳅的LD同工酶具有明显的组织特异性和发育阶段特异性.  相似文献   

16.
黄颡鱼不同组织中同工酶的表达模式   总被引:14,自引:0,他引:14  
采用聚丙烯酰胺凝胶电泳(PAGE)及特异性组织化学染色技术,研究了黄颡鱼成体眼、心脏、肾脏、肝脏、肌肉及尾鳍等6种组织中的4种同工酶(LDH、MDH、SOD、EST)分化表达模式。结果表明,黄颡鱼的同工酶EST、SOD、MDH具有明显的组织特异性,LDH无明显的组织差异,并对同工酶的组织表达特异性的生理意义进行分析和讨论。  相似文献   

17.
Summary Aldolase and pyruvate kinase isozymes were investigated in cultured hepatocytes from fetal, regenerating, and 2-acetyl-aminofluorene-fed rat liver as well as in some epithelial liver cell lines. Our results show that: (a) cell proliferation and prolonged expression of specific isozymes were found only in cultured hepatocytes from 17-day old fetuses; (b) the fetal type of pyruvate kinase expressed in regenerating and carcinogen-treated liver was temporarily lost only in cultured hepatocytes from regenerating liver; (c) the adult type of aldolase and pyruvate kinase was absent in one epithelial cell line derived from a carcinogen-treated liver and in the hepatoma tissue cell (HTC) line but was found in the Faza clone of the Reuber H35 cell line during the 50 first passages in vitro; and (d) the isozyme pattern of pyruvate kinase was always more strongly shifted than that of aldolase. The observations suggest that: (a) hepatocytes from carcinogen-treated liver exhibit the same lack of ability to proliferate in primary culture as normal adult hepatocytes; (b) adult hepatocytes can produce fetal isozymes without prior cell division; (c) pyruvate kinase is a stronger marker of dedifferentiation (retrodifferentiation) than aldolase; and (d) regulatory processes of isozyme expression are different during ontogenesis, regeneration, and hepatocarcinogenesis. This work was supported by the “Institut National de la Santé et de la Recherche Médicale” and the “Fondation pour la Recherche Medicale Fran?aise”  相似文献   

18.
Interspecific genetic differences in malate dehydrogenase (MDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and esterase (EST) isozymes in carp (Cyprinus carpio) and goldfish (Carassius auratus) were used to examine the allelic expressions in the hybrid between these species. A unique liver SOD and muscle LDH phenotype unambiguously identifies all presumed hybrid individuals. There was no evidence of F2 or backcross phenotypes in hybrid individuals. Liver MDH and EST phenotypes in hybrids show a preferential expression of goldfish isozymes. Variation in the levels of carp liver MDH isozymes may result from the polymorphism of a regulatory mutation affecting isozyme expression, leading to gene silencing after duplication.This work was supported through NSERC (Canada) grants to James P. Bogart and John F. Leatherland.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号