从贯叶连翘叶提取金丝桃素的工艺探讨

对从贯叶连翘中提取金丝桃素的工艺条件进行了优化研究,结果表明,金丝桃素提取较佳工艺为：45℃的水浸泡2h后,加入0.5%NaOH,用75％甲醇在0.05Mpa下78℃提取5次,每次1.5h,溶媒量为5倍,浓缩温度与干燥温度为78℃。     

贯叶连翘生长发育规律的观察研究

目的:观察贯叶连翘的生长发育规律,为人工栽培提供参考。方法:人工育苗与野生植株移栽相结合,观察其生长发育规律。测定相应的生长指数(包括株高,叶片长、宽),分析其在人工栽培中的应用。结果与结论:贯叶连翘的种子发芽需要光照,覆土不宜过深;当年育苗生长缓慢,注意少施勤施肥;多年生植株具有明显的两个开花盛期,注意水肥供应,可实现两次收获。     

贯叶连翘的研究和应用现状

近年来,国内外对天然生物活性物质的研究和应用日趋活跃,含天然活性成分的植物提取物的贸易额也随之大幅度上升。继银杏叶提取物之后,国外厂商又通过各种渠道来我国寻找贯叶连翘及其提取物货源,由此引发了国内贯叶连翘资源的开发热潮。作者根据国内外文献及自己的研究...     

贯叶连翘抗抑郁研究新进展

贯叶连翘,又名圣约翰草,是传统中药材之一,圣约翰草在德国用于抗抑郁症已有几百年的历史。贯叶连翘提取物对轻度和中度抑郁症患者和动物模型都有很好疗效,最新药理学研究表明贯叶连翘提取物中抗抑郁的主要成分是贯叶金丝桃素。贯叶金丝桃素是神经递质5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)的非竞争性重吸收抑制剂,贯叶金丝桃素还可以抑制突触体对γ氨基丁酸(GABA)和L-谷氨酸(L-glu)的重吸收,其作用机理至今还不甚明了,研究表明它的作用很可能是通过提高突触体细胞内钠离子浓度或通过降低突触体内突触小泡的跨膜pH梯度实现的。     

贯叶连翘的试管快繁研究

以贯叶连翘的茎节等为外植体 ,进行植株再生和快速繁殖研究。结果表明在BA、NAA、IBA和 2 ,4 -D不同组合的MS或 1/ 2MS培养基上 ,以茎段为外植体的愈伤组织和试管苗易于诱导 ;在 1/ 2MS BA 1～ 1.5 (mg/l) NAA 0 .1～ 0 .5 (mg/l)的培养基中增殖速度快 ,繁殖系数高 ;在 1/ 2MS NAA(IBA) 0 .5～ 1(mg/l)培养基中均能诱导生根。     

贯叶连翘醇提条件的多指标优化

本研究采用超声波提取法,对贯叶连翘中总黄酮、金丝桃素类和贯叶金丝桃素等主要有效成分在醇提过程中的提取条件(溶媒种类、浓度、提取时间等)进行了考察。结果表明,以总黄酮和金丝桃素类化合物为指标,用65％～80％的乙醇水溶液超声提取30min,提取效率较高;以贯叶金丝桃素为指标,则以用75％～90％的甲醇提取30min效果较好。     

贯叶连翘挥发性化学成分研究

采用水蒸气蒸馏法提取中草药贯叶连翘(全草)挥发油。通过气相色谱/质谱联用技术测定了挥发油的化学成分,从中鉴定出75种化合物,其中百里香酚、桉油精、丁香油酚等酚类物质为首次检出。结果表明,贯叶连翘因产地不同、部位不同,其挥发油的成分有较大的差别。     

激素对贯叶连翘器官分化的影响

贯叶连翘 (ＨｙｐｅｒｉｃｕｍｐｅｒｆｏｒａｔｕｍＬ .)为多年生草本 ,中国民间主要用于止血、抗炎、妇科病等[1] ,欧洲民间用于治疗创伤也有相当长的历史。近年来 ,欧、美等国家和地区将其应用于抑郁症的治疗 ,取得了很好的疗效。 80年代后期 ,由于发现该植物体内含有显著抗     

贯叶连翘的水培及其代谢产物检测

水培可诱导贯叶连翘组培苗生根能力强,根活力也增加;生根苗在1/6MS培养液中培养6周后的金丝桃素(HP)、假金丝桃素(PHP)和贯叶金丝桃素(HF)含量分别比基质[腐质土 蛭石(1:1)]中培养的提高10.13%、16.00%和61.36%。     

从贯叶连翘中提取金丝桃素的工艺探讨

对从贯叶连翘中提取金丝桃素的工艺条件进行了优化研究 ,结果表明 ,金丝桃素提取较佳工艺为 :45℃的水浸泡 2 h后 ,加入 0 .5 % Na OH,用 75 %甲醇在 0 .0 5 Mpa下 78℃提取5次 ,每次 1 .5 h,溶媒量为 5倍 ,浓缩温度与干燥温度为 78℃     

贯叶连翘中金丝桃素的合成与积累研究进展

金丝桃素是贯叶连翘的主要药理活性成分。本文概述了金丝桃素的化学与生物合成途径,介绍了金丝桃素在贯叶连翘个体发育过程中的积累以及利用贯叶连翘的细胞和组织培养技术生产与积累金丝桃素的研究进展。最后,指出分子生物学和电子显微镜技术的发展为深入研究金丝桃素的产生和积累提供了有利工具。     

用贯叶金丝桃残渣栽培香菇的研究

用提取金丝桃素后的贯叶金丝桃残渣和锯木屑作培养基分别栽培香菇 ,比较了由两种培养基栽培香菇的生物学效率、香菇的可溶性蛋白含量和超氧化物歧化酶 (SOD)活力。结果表明 :用贯叶金丝桃残渣栽培的香菇有较高的生物学效率、其香菇可溶性蛋白含量和SOD活力亦高 ,说明贯叶金丝桃残渣适合栽培香菇 ,为栽培香菇开发了一种新的生物资源。     

Anti-Influenza A Virus Effect of Hypericum perforatum L. Extract

To study the antiviral effect of Hypericum perforatum L. extract (HPE) on influenza A virus (IAV) (H1N1) in vitro and in vivo. Cytopathic effect (CPE) and neutral red (NR) dye uptake were used to examine the antiviral effect of HPE on Madin Darby Canine Kidney (MDCK) cells which were infected with IAV in vitro. HPE was effective against influenza A virus (IAV) in vitro, with a 50% effective concentration (EC50) of 40 μg/mL. The mean 50% cytotoxic concentration (CC50) in the MDCK used in these experiments was 1.5 mg/mL. Ribavirin was run in parallel with EC50 values of 5.0 μg/mL; the mean CC50 for ribavirin was 520 μg/mL. Oral gavage administrations of HPE or ribavirin to mice infected with the IAV were highly effective in preventing death, slowing the decline of arterial oxygen saturation, inhibiting lung consolidation and reducing lung virus titers. The minimum effective dose of HPE in these studies was 31.25 mg/kg/day, which was administered twice daily for 5 d beginning 4 h prior to virus exposure. Below a dosage of 2000 mg/kg/day, almost all treated mice survived, which suggests that HPE is of low toxicity. Ribavirin's minimum effective dose was 40 mg/kg/day with the LD50 determined to be 200 mg/kg/day. Delay of the initiation of either HPE or ribavirin therapy, using approximately 1/3 LD50 dose each time, could still be protective as late as 48 h after exposure to the IAV. While both agents appeared to have similar efficacy against IAV infections, HPE was considered to be less toxic and may warrant further evaluation as a possible therapy for influenza.     

贯叶连翘总提取物对谷氨酸棒状杆菌和粪产碱杆菌的抗菌作用

报道了经光照和未经光照处理后贯叶连翘总提取物对谷氨酸棒状杆菌和粪产碱杆菌的作用,结果表明,总提取物对谷氨酸状杆菌有强烈的抑菌和杀菌作用,对粪产碱杆菌有抑菌作用,其抑菌或杀菌作用与其浓度有关,且不需光照。     

贯叶连翘总提取物对黄色短杆菌的抗菌作用

报道了贯叶连翘总提取物作用于黄色短杆菌并经光照和未光照处理后对该菌的抗菌作用,目的是探讨贯叶连翘总提取物在光照和非光照处理时抗菌作用的异同。采用光密度(OD)值、活菌数(CFU)、最低杀菌浓度(MBC)值检测方法,测定了在12h的培养过程中,提取物作用时黄色短杆菌的OD640nm值、CFU、MBC值。结果表明提取物对该菌有很强的抑菌和杀菌作用,且这两种作用与其浓度有关,不需光照。     

Toxic Effects of Pb on Anatomy and Hypericin Content in Hypericum perforatum L.

Lead (Pb) is one of the most common heavy metal contaminants in the environment. The present study was therefore undertaken to determine the effects of Pb on structural characteristics and hypericin production in Hypericum perforatum. Mature plants were treated with contaminated soil in seven treatments (75, 150, 300, 600, 800, 1000, and 1500 mg/kg Pb in soil) with three repeats per treatment every 14 days. Maximum observed Pb content in shoot parts was observed in the treatments with 600 and 1500 mg/kg Pb. The Pb concentration in roots was higher than in shoot parts, enhanced with increasing Pb concentration in the soil. In this study, Pb treatment significantly influenced the morphology, anatomy, and hypericin content in the plant. Anatomical characteristics of leaf, stem, and root affected by Pb contamination, as well as scanning electron microscopy (SEM) studies, revealed structural changes in stomata and epicuticular waxes. Under Pb toxicity, anatomical symptoms occurred in leaves, including increase in sizes of epidermal cells, mesophyll tissue, and diameter of stems and roots, as well as amplified vascular bundles and pith area. This, therefore, indicated that metal contamination can change the chemical composition of this plant. Maximum hypericin content was observed in the treatment containing 600 mg/kg Pb in soil, which then decreased.