植物挥发性物质在蚜虫寄主定位中的作用

本文综述了植物挥发性物质的化学性质、研究方法及其在蚜虫寄主定位中的作用     

The evolution of dispersal polymorphisms in insects: The influence of habitats,host plants and mates

Wing-dimorphic, delphacid planthoppers were used to test hypotheses concerning the effects of habitat persistence and architectural complexity on the occurrence of dispersal. For reasons concerning both the durational stability of the habitat and the reduced availability of mates, selection has favored high levels of dispersal in species occupying temporary habitats. Flightlessness predominates in species occupying persistent habitats, and is promoted by a phenotypic trade-off between reproductive success and flight capability. Wings are retained in tree-inhabiting species, probably for reasons concerning the more effective negotiation of three-dimensional habitats. In contrast, flightlessness is characteristic of those species inhabiting low profile host plants. For several delphacid genera, migratory species are larger than their sedentary congeners. Because body size and fecundity are positively related in planthoppers, the large body size observed in migratory taxa may result from selection for increased fecundity in colonizing species.     

小翅稻蝗(Oxya yezoensis Shiraki)翅多型现象浅释

对小翅稻蝗翅型构成的季节变化,长翅型、短翅型产卵前期间的差异及光周期对翅型分化的影响进行了调查,结果表明,小翅稻蝗存在着明显的翅多型现象,相对翅长(前翅长/后足腿节长)最长可达1.49,最短只有0.50,翅长呈连续性双模态分布。早期羽化的个体大多为长翅型,随着季节的推移,羽化成虫短翅率逐渐增加。长翅型雌成虫的产卵前期间为42.7±13.1日,短翅型为15.3±6.4日,短翅型雌成虫的产卵前期间显著短于长翅型。不同的光周期短翅成虫出现的比例不同,光照短于LD1212时,几乎全为短翅型,LD1410短翅率为66.8%,LD168则长翅率达62.1%,光照长于LD186时,几乎全为长翅型。     

Male production by apterous viviparae of the aphid Myzus persicae temporarily exposed to different scotoperiods

Male production by apterous viviparae of a holocyclic biotype of the green peach aphid, Myzus persicae, was induced by pre-natal and/or post-natal exposures to a long-night regime of 15 h dark per diem. When the apterae were exposed to three or more long nights immediately after birth and they subsequently developed under a short-night regime of 8 h dark per diem, they produced females (mostly alate viviparae) during the first 8–10 days of reproduction and a high proportion of males thereafter. When the apterae were exposed to two long nights immediately before their birth and to short nights thereafter, they produced relatively few females (mostly alate viviparae), and males were deposited already after 4–6 days of reproduction, i.e. 16–18 days after the first exposure to the long nights. The proportion of males among the progeny of these apterae was highest when the two prenatal exposures comprised scotophases of 11–15 h; under such long-night regimes many aphids switched to producing males exclusively. To achieve this effect, the two long scotophases had to be separated by a photophase of more than 1–2 h. Fewer males were produced and most of the apterae reverted to the production of females (apterous viviparae) when the duration of the two prenatal scotophases was 9 h 45 min-10 h 30 min, or 18 h and longer.One long night of 15–39 h could also induce temporary male production if the aphids were exposed to it late in the 4th larval instar or as teneral adults.     

环境因素对长颚斗蟋翅型分化的影响

长颚斗蟋具有明显的翅二型现象。比较长颚斗蟋不同翅型成虫的形态差异,结果表明其短翅型成虫除翅已明显退化外,与长翅型成虫并无其他形态差异。为探究环境因素与长颚斗蟋翅型分化的关系,就光周期、温度及密度对其翅型分化的影响进行了研究。结果表明,短光周期会抑制其长翅型的形成,而LD16∶8h的长光周期则促进长翅个体的分化,但非自然条件的长光周期及全明或全暗条件与上述结论并不一致。变化光周期亦会影响其翅型分化,而随着改变光周期的时间及方向的不同,影响作用亦不相同。在孵化后第20天及40天经历光周期的趋长变化会促进其长翅化;而在孵化后第20天经历光周期的趋短变化则会抑制其长翅化,第30天转移却有促进作用。此外,低温(20℃)条件诱导长颚斗蟋短翅化,高温(25℃,30℃)则促进长翅型的分化。单独饲养时,LD12∶12h与LD16∶8h条件下的若虫均羽化为短翅型成虫,当密度增加至2或5头/容器时,成虫的长翅率均明显增加,说明高密度亦是长翅型个体出现的重要诱因。     

STUDIES ON THE PHYLOGENY AND HOST PLANT ASSOCIATION OF LACHNIDAE (HOMOPTERA: APHIDINEA)*

Abstract With reference to the material related to the evolution of host-plant associations, phylogenetic analyses of aphids in Lachnidae are carried out by using 20 taxa and 26 characters. The monophyly of Traminae and Cinarinae are recognized. However, Lachninae is proved to be a polyphyletic group. Three traditional subfamilies, Cinarinae, Lachninae and Traminae are still retained. Three tribes within Cinarinae which include Cinarini, Eulachnini and Schizolachnini are suggested. It is concluded that the ancestral host plants of Lachnidae were deciduous plants rather than coniferous trees or other plants.     

大蚜科系统发育及与寄主植物关系演变的研究(同翅目:蚜虫类)(英文)

本文根据大蚜科２０个代表属的２６个形态学及生态学特征对大蚜科系统发育及与寄主植物演变关系进行了研究。确认了传统的长足大蚜亚科及长跗蚜亚科为单系群,传统的大蚜亚科为多系群。建议保留传统的三个亚科,即长足大蚜亚科、长跗蚜亚科和大蚜亚科。在长足大蚜亚科中重新进行了族的划分,即长足大蚜亚科包括长足大蚜族、细长大蚜族和钝喙大蚜族。另外,推测大蚜科祖先共同的原始寄主植物为阔叶林,而非针叶林或其它类植物。     

Testing host‐associated differentiation in a quasi‐endophage and a parthenogen on native trees

Host‐associated differentiation (HAD) is the formation of genetically divergent host‐associated sub‐populations. Evidence of HAD has been reported for multiple insect herbivores to date, but published studies testing more than one herbivore for any given host‐plant species pair is limited to herbivores on goldenrods. This limits the number of pair‐wise comparisons that can be made about insect life‐history traits that might facilitate or inhibit host‐race development in general. Two traits previously proposed to facilitate HAD include endophagy and parthenogenesis. We tested for HAD in two herbivores, a quasi‐endophagous caterpillar and a parthenogenetic aphid, feeding on two closely related species of hickories. We found that the quasi‐endophage is panmictic, whereas the parthenogen exhibits HAD on their sympatric host plants, pecan and water hickory, at a geographic mesoscale. This is an important first step in the characterization of HAD in multiple insect herbivores using North American hickories, a host‐plant system with many shared parthenogens.     

OLFACTORY RESPONSES OF LYSIPHLEBIA JAPONICA TO VOLATILE CHEMICALS AND FRESH LEAVES OF THE HOST PLANTS OF COTTON APHIDS IN OLFACTOMETER

Abstract The behavioral responses of the female adult parasitoids Lysiphlebia japonica to plant volatile chemicals, host plants and cotton aphids were tested in a four arm air flow olfactometer. The results indicated that the female parasitoids were strongly attracted by the odors from cotton aphids and their host plants. Damaged cotton leaves were more attractive than intact ones. Among the four olfactory stimuli from the plant volatile chemicals dissolved in liquid paraffin oil at the concentration of 0.01 (volume fraction), the female parasitoids only positively responded to benzaldehyde, but did not show any preference to β-caryophyllene and β-pinene. Although heptanol elicited the highest EAC response of the parasitoids, the behavioral test showed that it repelled the parasitoids, and β-pinene shared the same feature with heptanol at the concentration of 1 (volume fraction). Our results also showed that the female parasitoids only preferred the cotton leaf from which the mummified aphids were collected, and did not express any interest to the three plants of melon family in the olfactometer, The possible mechanisms are discussed.     

香港地区蚜虫物种多样性

蚜虫是半翅目中一类体型较小的昆虫,广布于世界各地,其中绝大部分是农林害虫。目前,国内许多地区已开展蚜虫物种多样性和区系研究,但香港地区因蚜虫研究基础薄弱,至今尚未开展过系统研究。作者研究和整理了英国自然历史博物馆和中国科学院动物研究所动物标本馆已有的香港地区蚜虫标本,并参考大量相关文献,从分类阶元、特有物种、动物地理区划和寄主植物四个方面分析了香港地区蚜虫物种多样性。研究发现香港地区已知蚜虫7科44属70种,分别占中国已知科、属、种的53.85%、16.60%和6.83%,多样性较为丰富,其中有香港地区特有种6种。香港地区在动物地理区划中位于东洋界华南区闽广沿海亚区,蚜虫物种以分布于东洋界、古北界 东洋界、东洋界 澳洲界成分为主。现记载香港地区共有蚜虫的寄主植物30科58属,其中蚜科的寄主植物最为丰富,其次为扁蚜科和斑蚜科;寄主植物中寄生蚜虫类群最多的3个科分别为:禾本科、桑科和樟科。虽然香港地区面积仅占中国总面积的万分之一,但该地区复杂的地形地貌、适宜的气候环境以及丰富的植被共同构成了有利于蚜虫生存的小生境,因此蚜虫物种多样性较高,这也是我国南方地区蚜虫物种丰富的鲜明体现。     

Host plant mediation of diapause induction in the cabbage beetle, Colaphellus bowringi Baly （Coleoptera： Chrysomelidae）

The cabbage beetle, Colaphellus bowringiBaly （Coleoptera： Chrysomelidae）, is a serious pest of crucifers in China, undergoing an imaginal summer and winter diapause in the soil. The effects of host plants on diapause incidence were tested in the beetle. The ratio of adults entering diapause was significantly low when they fed on the mature leaves of Chinese cabbage Shanghaiqin （Brassica chinesis var communis） than those feeding on Chinese cabbage Suzhouqin （Brassica chinesis var communis）, radish （Raphanus sativus var longipinnatus） and stem mustard （Brassicajuncea vat tumida） at 25℃ combined with 13：11 （L： D） h. Fewer adults entered diapause on young leaves compared to physiologically aged and mature radish leaves at 25℃ combined with 13：11 （L： D） h. The effect of host plant species on diapause induction was also evident under continuously dark rearing conditions or at different photoperiods. These experimental results demonstrate that host plant mediation of diapause induction exists in the cabbage beetle. However, at temperatures ≤20℃ or photoperiods of 16：8 （L： D） h combined with 25℃, all individuals entered diapause regardless of the host plants, indicating that the effects of host plants on diapause induction could be expressed only within a limited range of temperatures and photoperiods.     

棉蚜茧蜂对某些挥发性次生化合物及棉蚜寄生植物的嗅觉反应(英)

本文利用四臂嗅觉计测定了雌性棉蚜茧蜂Lysiphlebia japonica对棉蚜四种寄主植物新鲜叶片、棉蚜和几种挥发性次生化合物的嗅觉反应,结果表明新鲜棉叶片和棉蚜的气味对棉蚜茧蜂有强烈的吸引作用,两者相混合使吸引作用明显增强;切碎的叶片比未切碎的叶片更具吸引作用。所测试的四种挥发性物质β-caryophyllene、heptanol、benzaldehyde和β-pinene,在浓度为0.01(体积分数)时,只有benzaldebyde对棉蚜茧蜂具有吸引作用,β-caryopbyllene和β-pinene对棉蚜茧蜂的吸引作用不明显,而heptanol对该蜂具有排斥作用。β-pinene在浓度为1(体积分数)时,也对该蜂具有排斥作用。对于棉蚜的四种寄主植物棉花、黄瓜、南瓜和丝瓜的完整叶片,棉蚜茧蜂只对棉花叶片产生定向反应,而对其它三种瓜类叶片没有反应。根据上述结果可以推知嗅觉在寄生蜂的寄主寻找过程中起着重要作用,绿叶气味物质和寄主气味可能是寄生蜂寻找寄主的远距离化学线索。棉蚜茧蜂对寄主定向是识别了特定化学指纹图谱,单一的某种化合物在寄主定向过程中所起的作用并不象预测的那样重要,而且组成图谱的物质并不是简单地混合在一起,而是相互作用,或加强或抑制。羽化前经历能够对寄生蜂的行为产生较大影响。     

Natural enemy ravine revisited: the importance of sample size for determining population growth

Abstract. 1. The population growth of three aphid species, Metopolophium dirhodum (Walker), Rhopalosiphum padi (L.), and Sitobion avenae (F.), on winter wheat, was analysed by regression. The calculations were based on censuses of aphids made in 268 plots at 3- or 7-day intervals for 10 years on leaves and 6 years on ears. The calculations were made separately for each plot each year, then repeated on the pooled data from all plots monitored in a year.
2. At the level of individual plots, no population growth was detected at very low densities. At high densities, the populations grew exponentially and the growth rates did not decrease with increasing aphid density.
3. Significant growth was always detected in the pooled data. These growth rates decreased significantly at the highest densities. Field estimates of the intrinsic rate of increase derived from these data ranged from 0.010 to 0.026 for M. dirhodum , 0.0071–0.011 for R. padi , and 0.00078–0.0061 and 0.0015–0.13 for S. avenae , on leaves and ears respectively .
4. The apparent lack of growth in the individual plots at low densities is attributable to small sample size. It is concluded that the natural enemy ravine in the population dynamics of cereal aphids, identified by Southwood and Comins (1976), is a consequence of low population densities at which population increase is undetectable unless very large samples are taken.     

植物病媒昆虫的翅型分化

翅多型现象是昆虫非遗传多型性的一种表现,包括不具飞行能力的短翅型或无翅型,以及可以进行长距离迁飞的长翅型或有翅型。翅多型现象常发生在可以携带病原并将其传播给植物宿主的媒介昆虫中,对植物病害的时空分布与暴发有重要影响。本文从翅型分化的遗传规律、诱导因素、分子机制和伴随翅型分化的其他生理表现4个方面,对植物病原主要传播媒介蚜虫和飞虱的翅型分化研究进行综述和梳理。昆虫翅型分化的诱导因素主要包括温度、湿度和光周期等非生物因素以及虫口密度、宿主营养、病毒等生物因素;而其内在的分子机制大多是通过胰岛素/胰岛素样生长因子信号(IIS)通路、c-Jun氨基末端激酶(c-Jun NH₂-terminal kinase, JNK)信号通路、Wingless和嗅觉受体SaveOrco等调控。翅型分化的同时伴随着生理状态的变化,表现为短翅型具有更强的繁殖能力和长翅型含有更丰富的飞行肌结构成分。目前,昆虫翅型分化的研究尚不够完善,有许多需要解答的问题,如找到胰岛素/胰岛素样生长因子信号通路中真正发挥功能的靶基因,JNK如何调控翅型分化以及虫媒病毒影响媒介昆虫翅型的分子机理。本综述可为控制虫媒病原的传播以及其他昆虫翅多型的研究提供参考。     

The effect of plant phenology on the induction of alatae and the development of populations of Metopolophium dirhodum (Walker), the rose-grain aphid, on winter wheat

In field experiments apterous Metopolophium dirhodum produced significantly more alatae when reared on mature plants (milky ripe stage) than on young plants. Nymphal and adult survival and relative growth rate were little affected by the developmental stage of the host plant. Relative growth rate and fecundity were strongly related to temperature. Laboratory experiments confirmed that the developmental stage of wheat had no significant effect on survival or relative growth rate of the aphids. However, laboratory grown plants did not produce the levels of alate production seen in field crops and in the field experiments. It is suggested that in investigations into the effects of the host plant on cereal aphids field grown plants should be used whenever possible. Alate production is proposed as the major plant induced factor affecting the development of M. dirhodum populations on wheat in the field. Alatae developed on mature plants even when the number of aphids was very low.